• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. Mar 1968; 47(3): 624–632.
PMCID: PMC297208

In vitro synthesis of immunoglobulin by rheumatoid synovial membrane

Abstract

A technique for the in vitro culture of rheumatoid synovial tissue with 14C-amino acids and isolation and quantitation of the newly synthesized immunoglobulins has been developed. This technique has been used to compare immunoglobulin synthesis of 12 rheumatoid synovia with that of synovia from nonarthritic patients and with that of normal human lymph nodes and spleen. In addition, the spleen of a patient with Felty's syndrome has also been examined. Immunoglobulin synthesis in rheumatoid synovia has been shown to be quantitatively and qualitatively similar to that of normal human spleen and lymph nodes although somewhat less active than the Felty's syndrome spleen examined. 79% of the immunoglobulin produced in rheumatoid synovia was of the IgG type, whereas IgM comprised 10% and IgA, 11% of the total. Less than 10% of the IgM synthesized was found to be rheumatoid factor. A fraction containing approximately 90% of its radioactivity in the form of IgG has been obtained for further studies.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • MELLORS RC, NOWOSLAWSKI A, KORNGOLD L, SENGSON BL. Rheumatoid factor and the pathogenesis of rheumatoid arthritis. J Exp Med. 1961 Feb 1;113:475–484. [PMC free article] [PubMed]
  • HOLLANDER JL, MCCARTY DJ, Jr, ASTORGA G, CASTRO-MURILLO E. STUDIES ON THE PATHOGENESIS OF RHEUMATOID JOINT INFLAMMATION. I. THE "R.A. CELL" AND A WORKING HYPOTHESIS. Ann Intern Med. 1965 Feb;62:271–280. [PubMed]
  • PEKIN TJ, Jr, ZVAIFLER NJ. HEMOLYTIC COMPLEMENT IN SYNOVIAL FLUID. J Clin Invest. 1964 Jul;43:1372–1382. [PMC free article] [PubMed]
  • FOSTIROPOULOS G, AUSTEN KF, BLOCH KJ. TOTAL HEMOLYTIC COMPLEMENT (CH50) AND SECOND COMPONENT OF COMPLEMENT (C2 HU) ACTIVITY IN SERUM AND SYNOVIAL FLUID. Arthritis Rheum. 1965 Apr;8:219–232. [PubMed]
  • AIDEM HP, BAKER LD. SYNOVECTOMY OF THE KNEE JOINT IN RHEUMATOID ARTHRITIS. JAMA. 1964 Jan 4;187:4–6. [PubMed]
  • ROPES MW, BENNETT GA, COBB S, JACOX R, JESSAR RA. 1958 Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis. 1958 Dec;9(4):175–176. [PubMed]
  • EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. [PubMed]
  • LOSPALLUTO J, CHEGORIANSKY J, LEWIS A, ZIFF M. Chromatographic properties of gamma globulin: behavior of serum gamma macroglobulins. J Clin Invest. 1960 Mar;39:473–480. [PMC free article] [PubMed]
  • HEREMANS JF, VAERMAN JP. Beta-2A-Globulin as a possible carrier of allergic reaginic activity. Nature. 1962 Mar 17;193:1091–1092. [PubMed]
  • SINGER JM, PLOTZ CM, GOLDBERG R. THE DETECTION OF ANTI-GLOBULIN FACTORS UTILIZING PRE-COATED LATEX ARTICLES. Arthritis Rheum. 1965 Apr;8:194–202. [PubMed]
  • LOSPALLUTO J, ZIFF M. Chromatographic studies of the rheumatoid factor. J Exp Med. 1959 Aug 1;110(2):169–186. [PMC free article] [PubMed]
  • BIANCHI FA, KEECH MK. Comparison of two slide tests in rheumatoid arthritis. JAMA. 1963 Jul 27;185:318–321. [PubMed]
  • HELLER G, JACOBSON AS, KOLODNY MH. A modification of the hemagglutination test for rheumatoid arthritis. Proc Soc Exp Biol Med. 1949 Nov;72(2):316–323. [PubMed]
  • CHRISTIAN CL. RHEUMATOID ARTHRITIS--ETIOLOGIC CONSIDERATIONS. Arthritis Rheum. 1964 Oct;7:455–466. [PubMed]
  • ZIFF M. Genetics, hypersensitivity and the connective tissue diseases. Am J Med. 1961 Jan;30:1–7. [PubMed]
  • Allen JC, Kunkel HG. Hidden rheumatoid factors with specificity for native gamma globulins. Arthritis Rheum. 1966 Dec;9(6):758–768. [PubMed]
  • EDELMAN GM, KUNKEL HG, FRANKLIN EC. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. [PMC free article] [PubMed]
  • SOLOMON A, WALDMANN TA, FAHEY JL. Clinical and experimental metabolism of normal 6.6s gamma-globulin in normal subjects and in patients with macroglobulinemia and multiple myeloma. J Lab Clin Med. 1963 Jul;62:1–17. [PubMed]
  • TOMASI TB, Jr, TAN EM, SOLOMON A, PRENDERGAST RA. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. [PMC free article] [PubMed]
  • CRABBE PA, CARBONARA AO, HEREMANS JF. THE NORMAL HUMAN INTESTINAL MUCOSA AS A MAJOR SOURCE OF PLASMA CELLS CONTAINING GAMMA-A-IMMUNOGLOBULIN. Lab Invest. 1965 Mar;14:235–248. [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...