• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. Dec 1991; 88(6): 1976–1981.
PMCID: PMC295781

Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome.

Abstract

Acute Respiratory Distress Syndrome (ARDS) is characterized by lung injury and damage to the alveolar type II cells. This study sought to determine if endogenous surfactant is altered in ARDS. Bronchoalveolar lavage was performed in patients at-risk to develop ARDS (AR, n = 20), with ARDS (A, n = 66) and in normal subjects (N, n = 29). The crude surfactant pellet was analyzed for total phospholipids (PL), individual phospholipids, SP-A, SP-B, and minimum surface tension (STmin). PL was decreased in both AR and A (3.48 +/- 0.61 and 2.47 +/- 0.40 mumol/ml, respectively) compared to N (7.99 +/- 0.60 mumol/ml). Phosphatidylcholine was decreased in A (62.64 +/- 2.20% PL) compared to N (76.27 +/- 2.05% PL). Phosphatidylglycerol was 11.58 +/- 1.21% PL in N and was decreased to 6.48 +/- 1.43% PL in A. SP-A was 123.64 +/- 20.66 micrograms/ml in N and was decreased to 49.28 +/- 21.68 micrograms/ml in AR and to 29.88 +/- 8.49 micrograms/ml in A. SP-B was 1.28 +/- 0.33 micrograms/ml in N and was decreased to 0.57 +/- 0.24 micrograms/ml in A. STmin was increased in AR (15.1 +/- 2.53 dyn/cm) and A (29.04 +/- 2.05 dyn/cm) compared to N (7.44 +/- 1.61 dyn/cm). These data demonstrate that the chemical composition and functional activity of surfactant is altered in ARDS. Several of these alterations also occur in AR, suggesting that these abnormalities occur early in the disease process.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ashbaugh DG, Bigelow DB, Petty TL, Levine BE. Acute respiratory distress in adults. Lancet. 1967 Aug 12;2(7511):319–323. [PubMed]
  • Petty TL, Reiss OK, Paul GW, Silvers GW, Elkins ND. Characteristics of pulmonary surfactant in adult respiratory distress syndrome associated with trauma and shock. Am Rev Respir Dis. 1977 Mar;115(3):531–536. [PubMed]
  • Petty TL, Silvers GW, Paul GW, Stanford RE. Abnormalities in lung elastic properties and surfactant function in adult respiratory distress syndrome. Chest. 1979 May;75(5):571–574. [PubMed]
  • Hallman M, Spragg R, Harrell JH, Moser KM, Gluck L. Evidence of lung surfactant abnormality in respiratory failure. Study of bronchoalveolar lavage phospholipids, surface activity, phospholipase activity, and plasma myoinositol. J Clin Invest. 1982 Sep;70(3):673–683. [PMC free article] [PubMed]
  • Bachofen M, Weibel ER. Alterations of the gas exchange apparatus in adult respiratory insufficiency associated with septicemia. Am Rev Respir Dis. 1977 Oct;116(4):589–615. [PubMed]
  • Fantone JC, Ward PA. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol. 1982 Jun;107(3):395–418. [PMC free article] [PubMed]
  • Rinaldo JE, Rogers RM. Adult respiratory-distress syndrome: changing concepts of lung injury and repair. N Engl J Med. 1982 Apr 15;306(15):900–909. [PubMed]
  • Tate RM, Repine JE. Neutrophils and the adult respiratory distress syndrome. Am Rev Respir Dis. 1983 Sep;128(3):552–559. [PubMed]
  • Fuchimukai T, Fujiwara T, Takahashi A, Enhorning G. Artificial pulmonary surfactant inhibited by proteins. J Appl Physiol (1985) 1987 Feb;62(2):429–437. [PubMed]
  • Ikegami M, Jobe A, Jacobs H, Lam R. A protein from airways of premature lambs that inhibits surfactant function. J Appl Physiol Respir Environ Exerc Physiol. 1984 Oct;57(4):1134–1142. [PubMed]
  • Holm BA, Notter RH. Effects of hemoglobin and cell membrane lipids on pulmonary surfactant activity. J Appl Physiol (1985) 1987 Oct;63(4):1434–1442. [PubMed]
  • SAID SI, AVERY ME, DAVIS RK, BANERJEE CM, EL-GOHARY M. PULMONARY SURFACE ACTIVITY IN INDUCED PULMONARY EDEMA. J Clin Invest. 1965 Mar;44:458–464. [PMC free article] [PubMed]
  • Albert RK, Lakshminarayan S, Hildebrandt J, Kirk W, Butler J. Increased surface tension favors pulmonary edema formation in anesthetized dogs' lungs. J Clin Invest. 1979 May;63(5):1015–1018. [PMC free article] [PubMed]
  • Bredenberg CE, Paskanik AM, Nieman GF. High surface tension pulmonary edema. J Surg Res. 1983 Jun;34(6):515–523. [PubMed]
  • Jefferies AL, Kawano T, Mori S, Burger R. Effect of increased surface tension and assisted ventilation on 99mTc-DTPA clearance. J Appl Physiol (1985) 1988 Feb;64(2):562–568. [PubMed]
  • BLIGH EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. [PubMed]
  • Peterson GL. Determination of total protein. Methods Enzymol. 1983;91:95–119. [PubMed]
  • Fine JB, Sprecher H. Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates. J Lipid Res. 1982 May;23(4):660–663. [PubMed]
  • Mason RJ, Nellenbogen J, Clements JA. Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res. 1976 May;17(3):281–284. [PubMed]
  • Tsai MY, Cain M, Josephson MW. Improved thin-layer chromatography of disaturated phosphatidylcholine in amniotic fluid. Clin Chem. 1981 Feb;27(2):239–242. [PubMed]
  • Ross GF, Notter RH, Meuth J, Whitsett JA. Phospholipid binding and biophysical activity of pulmonary surfactant-associated protein (SAP)-35 and its non-collagenous COOH-terminal domains. J Biol Chem. 1986 Oct 25;261(30):14283–14291. [PubMed]
  • Ross GF, Ohning BL, Tannenbaum D, Whitsett JA. Structural relationships of the major glycoproteins from human alveolar proteinosis surfactant. Biochim Biophys Acta. 1987 Feb 25;911(3):294–305. [PubMed]
  • McMahan MJ, Mimouni F, Miodovnik M, Hull WM, Whitsett JA. Surfactant associated protein (SAP-35) in amniotic fluid from diabetic and nondiabetic pregnancies. Obstet Gynecol. 1987 Jul;70(1):94–98. [PubMed]
  • Whitsett JA, Hull WM, Ohning B, Ross G, Weaver TE. Immunologic identification of a pulmonary surfactant-associated protein of molecular weight = 6000 daltons. Pediatr Res. 1986 Aug;20(8):744–749. [PubMed]
  • Heinrikson RL, Meredith SC. Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. Anal Biochem. 1984 Jan;136(1):65–74. [PubMed]
  • Bidlingmeyer BA, Cohen SA, Tarvin TL. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr. 1984 Dec 7;336(1):93–104. [PubMed]
  • Edman P, Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. [PubMed]
  • Hewick RM, Hunkapiller MW, Hood LE, Dreyer WJ. A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981 Aug 10;256(15):7990–7997. [PubMed]
  • Olafson RW, Rink U, Kielland S, Yu SH, Chung J, Harding PG, Possmayer F. Protein sequence analysis studies on the low molecular weight hydrophobic proteins associated with bovine pulmonary surfactant. Biochem Biophys Res Commun. 1987 Nov 13;148(3):1406–1411. [PubMed]
  • Weaver TE, Sarin VK, Sawtell N, Hull WM, Whitsett JA. Identification of surfactant proteolipid SP-B in human surfactant and fetal lung. J Appl Physiol (1985) 1988 Aug;65(2):982–987. [PubMed]
  • Enhorning G. Pulsating bubble technique for evaluating pulmonary surfactant. J Appl Physiol Respir Environ Exerc Physiol. 1977 Aug;43(2):198–203. [PubMed]
  • Tomashefski JF., Jr Pulmonary pathology of the adult respiratory distress syndrome. Clin Chest Med. 1990 Dec;11(4):593–619. [PubMed]
  • Pison U, Seeger W, Buchhorn R, Joka T, Brand M, Obertacke U, Neuhof H, Schmit-Neuerburg KP. Surfactant abnormalities in patients with respiratory failure after multiple trauma. Am Rev Respir Dis. 1989 Oct;140(4):1033–1039. [PubMed]
  • Pison U, Obertacke U, Brand M, Seeger W, Joka T, Bruch J, Schmit-Neuerburg KP. Altered pulmonary surfactant in uncomplicated and septicemia-complicated courses of acute respiratory failure. J Trauma. 1990 Jan;30(1):19–26. [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...