• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Oct 1980; 144(1): 114–123.
PMCID: PMC294601

Are growth rates of Escherichia coli in batch cultures limited by respiration?

Abstract

Batch cultures of Escherichia coli were grown in minimal media supplemented with various carbon sources which supported growth at specific growth rates from 0.2 to 1.3/h. The respiration rates of the cultures were measured continuously. With few exceptions, the specific rate of oxygen consumption was about 20 mmol of O2/h per g (dry weight), suggesting that the respiratory capacity was limited at this value. The adenosine triphosphate (ATP) required for the production of cell material from the different carbon sources was calculated on the basis of known ATP requirements in the biochemical pathways and routes of macromolecular synthesis. The calculated ATP requirements, together with the measured growth rates and growth yields on the different carbon sources, were used to calculate the rate of ATP synthesis by oxidative phosphorylation. This rate was closely related to the respiration rate. We suggest that aerobic growth of E. coli in batch cultures is limited by the rate of respiration and the concomitant rate of ATP generation through oxidative phosphorylation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Andersen KB, von Meyenburg K. Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. J Biol Chem. 1977 Jun 25;252(12):4151–4156. [PubMed]
  • Beck C, von Meyenburg HK. Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation. J Bacteriol. 1968 Aug;96(2):479–486. [PMC free article] [PubMed]
  • de Vries W, Kapteijn WM, van der Beek EG, Stouthamer AH. Molar growth yields and fermentation balances of Lactobacillus casei L3 in batch cultures and in continuous cultures. J Gen Microbiol. 1970 Nov;63(3):333–345. [PubMed]
  • Du Preez JC, Lategan PM. Gas chromatographic determination of C2-C5 fatty acids in aqueous media with a Porapak N column. J Chromatogr. 1976 Sep 1;124(1):63–65. [PubMed]
  • Farmer IS, Jones CW. The energetics of Escherichia coli during aerobic growth in continuous culture. Eur J Biochem. 1976 Aug 1;67(1):115–122. [PubMed]
  • Forrest WW, Walker DJ. The generation and utilization of energy during growth. Adv Microb Physiol. 1971;5:213–274. [PubMed]
  • Hamilton ID, Holms WH. Measurement of respiration of micro-organisms during batch culture. Lab Pract. 1970 Aug;19(8):795–passim. [PubMed]
  • Hansen MT, Pato ML, Molin S, Fill NP, von Meyenburg K. Simple downshift and resulting lack of correlation between ppGpp pool size and ribonucleic acid accumulation. J Bacteriol. 1975 May;122(2):585–591. [PMC free article] [PubMed]
  • Harrison DE. The regulation of respiration rate in growing bacteria. Adv Microb Physiol. 1976;14(11):243–313. [PubMed]
  • Hempfling WP. Repression of oxidative phosphorylation in Escherichia coli B by growth in glucose and other carbohydrates. Biochem Biophys Res Commun. 1970 Oct 9;41(1):9–15. [PubMed]
  • Hempfling WP, Mainzer SE. Effects of varying the carbon source limiting growth on yield and maintenance characteristics of Escherichia coli in continuous culture. J Bacteriol. 1975 Sep;123(3):1076–1087. [PMC free article] [PubMed]
  • HERBERT D, ELSWORTH R, TELLING RC. The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol. 1956 Jul;14(3):601–622. [PubMed]
  • Hitchener BJ, Egan AF, Rogers PJ. Energetics of Microbacterium thermosphactum in glucose-limited continuous culture. Appl Environ Microbiol. 1979 Jun;37(6):1047–1052. [PMC free article] [PubMed]
  • Holms WH, Bennett PM. Regulation of isocitrate dehydrogenase activity in Escherichia coli on adaptation to acetate. J Gen Microbiol. 1971 Jan;65(1):57–68. [PubMed]
  • JULIAN GR. (14C)LYSINE PEPTIDES SYNTHESIZED IN AN IN VITRO ESCHERICHIA COLI SYSTEM IN THE PRESENCE OF CHLORAMPHENICOL. J Mol Biol. 1965 May;12:9–16. [PubMed]
  • KESSLER DP, RICKENBERG HV. The competitive inhibition of alpha-methylglucoside uptake in Escherichia coli. Biochem Biophys Res Commun. 1963 Mar 25;10:482–487. [PubMed]
  • Mainzer SE, Hempfling WP. Effects of growth temperature on yield and maintenance during glucose-limited continuous culture of Escherichia coli. J Bacteriol. 1976 Apr;126(1):251–256. [PMC free article] [PubMed]
  • Meijer EM, van Verseveld HW, van der Beek EG, Stouthamer AH. Energy conservation during aerobic growth in Paracoccus denitrificans. Arch Microbiol. 1977 Feb 4;112(1):25–34. [PubMed]
  • Neijssel OM, Tempest DW. Bioenergetic aspects of aerobic growth of Klebsiella aerogenes NCTC 418 in carbon-limited and carbon-sufficient chemostat culture. Arch Microbiol. 1976 Mar 19;107(2):215–221. [PubMed]
  • O'Brien TA, Schrock HL, Russell P, Blake R, 2nd, Gennis RB. Preparation of Escherichia coli pyruvate oxidase utilizing a thiamine pyrophosphate affinity column. Biochim Biophys Acta. 1976 Nov 8;452(1):13–29. [PubMed]
  • Rogers PJ, Stewart PR. Energetic efficiency and maintenance. Energy characteristics of Saccharomyces cerevisiae (wild type and petite) and Candida parapsilosis grown aerobically and micro-aerobically in continuous culture. Arch Microbiol. 1974;99(1):25–46. [PubMed]
  • Saier MH., Jr Bacterial phosphoenolpyruvate: sugar phosphotransferase systems: structural, functional, and evolutionary interrelationships. Bacteriol Rev. 1977 Dec;41(4):856–871. [PMC free article] [PubMed]
  • SCHAECHTER M, MAALOE O, KJELDGAARD NO. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. [PubMed]
  • Stouthamer AH. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie Van Leeuwenhoek. 1973;39(3):545–565. [PubMed]
  • Stouthamer AH, Bettenhaussen CW. Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes. Arch Microbiol. 1975 Mar 10;102(3):187–192. [PubMed]
  • van der Beek EG, Stouthamer AH. Oxidative phosphorylation in intact bacteria. Arch Mikrobiol. 1973;89(4):327–339. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...