Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jul 1980; 143(1): 313–320.
PMCID: PMC294235

Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae.


Two antibiotic-resistant isolates of Streptococcus pneumoniae were investigated for conjugative transfer of their drug resistance markers into streptococcal (groups B and D) and pneumococcal (encapsulated and non-encapsulataed) recipients. Of these, 7 wild-type donor pneumococci transferred all their resistance markers (except Pc [penicillin], Su [sulfonamide], and Tp [trimethoprim]) into group D Streptococcus and non-encapsulated S. pneumoniae recipients at a low frequency (10(-5) to 10(-6)). The resistance markers transferred were Tc (tetracycline); Tc and Cm (chloramphenicol); Tc and MLS (macrolides, lincosamides, and streptogramin B); Tc, MLS, Km (kanamycin), and Cm. The transconjugants obtained retransferred their resistance markers into appropriate streptococcal or pneumococcal recipients or both. The resistance markers of streptococcal transconjugants could not be cured by chemical agents. All attempts to detect extra-chromosomal deoxyribonucleic acid from pneumococcal or streptococcal transconjugants were unsuccessful. The molecular weight of a streptococcal conjugative R plasmid (pIP501) was investigated after transfer into the non-encapsulated S. pneumoniae recipient and was found to be similar to that of the wild-type group B Streptococcus host (20 x 10(6)).

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Appelbaum PC, Bhamjee A, Scragg JN, Hallett AF, Bowen AJ, Cooper RC. Streptococcus pneumoniae resistant to penicillin and chloramphenicol. Lancet. 1977 Nov 12;2(8046):995–997. [PubMed]
  • Clewell DB, Franke AE. Characterization of a plasmid determining resistance to erythromycin, lincomycin, and vernamycin Balpha in a strain Streptococcus pyogenes. Antimicrob Agents Chemother. 1974 May;5(5):534–537. [PMC free article] [PubMed]
  • Courvalin PM, Carlier C, Chabbert YA. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus". Ann Inst Pasteur (Paris) 1972 Dec;123(6):755–759. [PubMed]
  • Courvalin PM, Shaw WV, Jacob AE. Plasmid-mediated mechanisms of resistance to aminoglycoside-aminocyclitol antibiotics and to chloramphenicol in group D streptococci. Antimicrob Agents Chemother. 1978 May;13(5):716–725. [PMC free article] [PubMed]
  • Cybulska J, Jeljaszewicz J, Lund E, Munksgaard A. Prevalence of types of Diplococcus pneumoniae and their susceptibility to 30 antibiotics. Chemotherapy. 1970;15(5):304–316. [PubMed]
  • Dang-Van A, Tiraby G, Acar JF, Shaw WV, Bouanchaud DH. Chloramphenicol resistance in Streptococcus pneumoniae: enzymatic acetylation and possible plasmid linkage. Antimicrob Agents Chemother. 1978 Apr;13(4):577–583. [PMC free article] [PubMed]
  • Dunny GM, Craig RA, Carron RL, Clewell DB. Plasmid transfer in Streptococcus faecalis: production of multiple sex pheromones by recipients. Plasmid. 1979 Jul;2(3):454–465. [PubMed]
  • Goldstein FW, Dang Van A, Bouanchaud DH, Acar JF. Evolution de la résistance aux antibiotiques des pneumocoques et répartition de leurs types capsulaires. Pathol Biol (Paris) 1978 Mar-Apr;26(3-4):173–180. [PubMed]
  • Hansman D, Glasgow H, Sturt J, Devitt L, Douglas R. Increased resistance to penicillin of pneumococci isolated from man. N Engl J Med. 1971 Jan 28;284(4):175–177. [PubMed]
  • Hershfield V. Plasmids mediating multiple drug resistance in group B streptococcus: transferability and molecular properties. Plasmid. 1979 Jan;2(1):137–149. [PubMed]
  • Horodniceanu T, Bouanchaud DH, Bieth G, Chabbert YA. R plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1976 Nov;10(5):795–801. [PMC free article] [PubMed]
  • Horodniceanu T, Bougueleret L, El-Solh N, Bieth G, Delbos F. High-level, plasmid-borne resistance to gentamicin in Streptococcus faecalis subsp. zymogenes. Antimicrob Agents Chemother. 1979 Nov;16(5):686–689. [PMC free article] [PubMed]
  • Horodniceanu T, Bougueleret L, El-Solh N, Bouanchaud DH, Chabbert YA. Conjugative R plasmids in Streptococcus agalactiae (group B). Plasmid. 1979 Apr;2(2):197–206. [PubMed]
  • Jacob AE, Hobbs SJ. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. [PMC free article] [PubMed]
  • Jacobs MR, Koornhof HJ, Robins-Browne RM, Stevenson CM, Vermaak ZA, Freiman I, Miller GB, Witcomb MA, Isaäcson M, Ward JI, et al. Emergence of multiply resistant pneumococci. N Engl J Med. 1978 Oct 5;299(14):735–740. [PubMed]
  • Jorgensen JH, Lee JC, Alexander GA. Rapid penicillinase paper strip test for detection of beta-lactamase-producing Haemophilus influenzae and Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1977 Jun;11(6):1087–1088. [PMC free article] [PubMed]
  • Krogstad DJ, Korfhagen TR, Moellering RC, Jr, Wennersten C, Swartz MN. Plasmid-mediated resistance to antibiotic synergism in enterococci. J Clin Invest. 1978 Jun;61(6):1645–1653. [PMC free article] [PubMed]
  • Krogstad DJ, Korfhagen TR, Moellering RC, Jr, Wennersten C, Swartz MN. Aminoglycoside-inactivating enzymes in clinical isolates of Streptococcus faecalis. An explanation for resistance to antibiotic synergism. J Clin Invest. 1978 Aug;62(2):480–486. [PMC free article] [PubMed]
  • Malke H. Genetics of resistance to macrolide antibiotics and lincomycin in natural isolates of Streptococcus pyogenes. Mol Gen Genet. 1974;135(4):349–367. [PubMed]
  • Miyamura S, Ochiai H, Nitahara Y, Nakagawa Y, Terao M. Resistance mechanism of chloramphenicol in Streptococcus haemolyticus, Streptococcus pneumoniae and Streptococcus faecalis. Microbiol Immunol. 1977;21(2):69–76. [PubMed]
  • O'Callaghan CH, Morris A, Kirby SM, Shingler AH. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. [PMC free article] [PubMed]
  • Porter RD, Shoemaker NB, Rampe G, Guild WR. Bacteriophage-associated gene transfer in pneumococcus: transduction or pseudotransduction? J Bacteriol. 1979 Jan;137(1):556–567. [PMC free article] [PubMed]
  • Robins-Brown RM, Gaspar MN, Ward JI, Wachsmuth IK, Koornhof HJ, Jacobs MR, Thornsberry C. Resistance mechanisms of multiply resistant pneumococci: antibiotic degradation studies. Antimicrob Agents Chemother. 1979 Mar;15(3):470–474. [PMC free article] [PubMed]
  • Shoemaker NB, Smith MD, Guild WR. Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus. J Bacteriol. 1979 Aug;139(2):432–441. [PMC free article] [PubMed]
  • Shoemaker NB, Smith MD, Guild WR. DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in Pneumococcus. Plasmid. 1980 Jan;3(1):80–87. [PubMed]
  • Smith MD, Guild WR. A plasmid in Streptococcus pneumoniae. J Bacteriol. 1979 Feb;137(2):735–739. [PMC free article] [PubMed]
  • Smith MD, Shoemaker NB, Burdett V, Guild WR. Transfer of plasmids by conjugation in Streptococcus pneumonias. Plasmid. 1980 Jan;3(1):70–79. [PubMed]
  • Young FE, Mayer L. Genetic determinants of microbial resistance to antibiotics. Rev Infect Dis. 1979 Jan-Feb;1(1):55–63. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...