• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jun 1980; 142(3): 869–878.
PMCID: PMC294112

Changes in cell diameter during the division cycle of Escherichia coli.

Abstract

Extensive measurements of steady-state populations of several Escherichia coli strains have consistently indicated that cell diameter decreases with increasing cell length. This was observed both after electron microscopy of air-dried cells and after phase-contrast microscopy of living cells. The analysis was made by considering separately the unconstricted cells and three classes (slight, medium, and deep) of constricted cells in the population. During slow growth, cells with the average newborn length were up to 8% thicker than unconstricted cells twice as long. This decrease in diameter is less at higher growth rates. Despite the small changes and the large variation of the diameter in any particular length class, significant negative correlations between diameter and length were obtained. Cell diameter increases again at the end of the cell cycle as indicated by an increase of average diameter in the three consecutive classes of constriction.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Donachie WD, Begg KJ, Vicente M. Cell length, cell growth and cell division. Nature. 1976 Nov 25;264(5584):328–333. [PubMed]
  • Grover NB, Woldringh CL, Zaritsky A, Rosenberger RF. Elongation of rod-shaped bacteria. J Theor Biol. 1977 Jul 21;67(2):181–193. [PubMed]
  • Harvey RJ, Marr AG, Painter PR. Kinetics of growth of individual cells of Escherichia coli and Azotobacter agilis. J Bacteriol. 1967 Feb;93(2):605–617. [PMC free article] [PubMed]
  • Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. [PubMed]
  • Helmstetter CE, Pierucci O. DNA synthesis during the division cycle of three substrains of Escherichia coli B/r. J Mol Biol. 1976 Apr 15;102(3):477–486. [PubMed]
  • Koppes LJ, Overbeeke N, Nanninga N. DNA replication pattern and cell wall growth in Escherichia coli PAT 84. J Bacteriol. 1978 Mar;133(3):1053–1061. [PMC free article] [PubMed]
  • Koppes LH, Woldringh CL, Nanninga N. Size variations and correlation of different cell cycle events in slow-growing Escherichia coli. J Bacteriol. 1978 May;134(2):423–433. [PMC free article] [PubMed]
  • Kubitschek HE. Constancy of the ratio of DNA to cell volume in steady-state cultures of Escherichia coli B-r. Biophys J. 1974 Feb;14(2):119–123. [PMC free article] [PubMed]
  • Marr AG, Harvey RJ, Trentini WC. Growth and division of Escherichia coli. J Bacteriol. 1966 Jun;91(6):2388–2389. [PMC free article] [PubMed]
  • Meacock PA, Pritchard RH, Roberts EM. Effect of thymine concentration on cell shape in Thy- Escherichia coli B/r. J Bacteriol. 1978 Jan;133(1):320–328. [PMC free article] [PubMed]
  • Nanninga N, Koppes LJ, de Vries-Tijssen FC. The cell cycle of Bacillus subtilis as studied by electron microscopy. Arch Microbiol. 1979 Nov;123(2):173–181. [PubMed]
  • Pierucci O. Dimensions of Escherichia coli at various growth rates: model for envelope growth. J Bacteriol. 1978 Aug;135(2):559–574. [PMC free article] [PubMed]
  • Poole RK. Fluctuations in buoyant density during the cell cycle of Escherichia coli K12: significance for the preparation of synchronous cultures by age selection. J Gen Microbiol. 1977 Jan;98(1):177–186. [PubMed]
  • Previc EP. Biochemical determination of bacterial morphology and the geometry of cell division. J Theor Biol. 1970 Jun;27(3):471–497. [PubMed]
  • Pritchard RH. Review lecture on the growth and form of a bacterial cell. Philos Trans R Soc Lond B Biol Sci. 1974 Feb 21;267(886):303–336. [PubMed]
  • Rosenberger RF, Grover NB, Zaritsky A, Woldringh CL. Surface growth in rod-shaped bacteria. J Theor Biol. 1978 Aug 21;73(4):711–721. [PubMed]
  • SCHAECHTER M, MAALOE O, KJELDGAARD NO. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. [PubMed]
  • Sedgwick EG, Paulton RJ. Dimension control in bacteria. Can J Microbiol. 1974 Feb;20(2):231–236. [PubMed]
  • Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. [PMC free article] [PubMed]
  • Verwer RW, Nanninga N, Keck W, Schwarz U. Arrangement of glycan chains in the sacculus of Escherichia coli. J Bacteriol. 1978 Nov;136(2):723–729. [PMC free article] [PubMed]
  • Woldringh CL. Morphological analysis of nuclear separation and cell division during the life cycle of Escherichia coli. J Bacteriol. 1976 Jan;125(1):248–257. [PMC free article] [PubMed]
  • Woldringh CL, de Jong MA, van den Berg W, Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. [PMC free article] [PubMed]
  • Zaritsky A. On dimensional determination of rod-shaped bacteria. J Theor Biol. 1975 Oct;54(2):243–248. [PubMed]
  • Zaritsky A, Pritchard RH. Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. J Bacteriol. 1973 May;114(2):824–837. [PMC free article] [PubMed]
  • Zaritsky A, Woldringh CL. Chromosome replication rate and cell shape in Escherichia coli: lack of coupling. J Bacteriol. 1978 Aug;135(2):581–587. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...