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PLoS One. 2010; 5(9): e12570.
Published online Sep 3, 2010. doi:  10.1371/journal.pone.0012570
PMCID: PMC2933237

Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans

Michael Otto, Editor


For Deinococcus radiodurans and other bacteria which are extremely resistant to ionizing radiation, ultraviolet radiation, and desiccation, a mechanistic link exists between resistance, manganese accumulation, and protein protection. We show that ultrafiltered, protein-free preparations of D. radiodurans cell extracts prevent protein oxidation at massive doses of ionizing radiation. In contrast, ultrafiltrates from ionizing radiation-sensitive bacteria were not protective. The D. radiodurans ultrafiltrate was enriched in Mn, phosphate, nucleosides and bases, and peptides. When reconstituted in vitro at concentrations approximating those in the D. radiodurans cytosol, peptides interacted synergistically with Mn2+ and orthophosphate, and preserved the activity of large, multimeric enzymes exposed to 50,000 Gy, conditions which obliterated DNA. When applied ex vivo, the D. radiodurans ultrafiltrate protected Escherichia coli cells and human Jurkat T cells from extreme cellular insults caused by ionizing radiation. By establishing that Mn2+-metabolite complexes of D. radiodurans specifically protect proteins against indirect damage caused by gamma-rays delivered in vast doses, our findings provide the basis for a new approach to radioprotection and insight into how surplus Mn budgets in cells combat reactive oxygen species.


Unrepaired DNA double-strand breaks (DSBs) are generally the cause of ionizing radiation-induced cell-killing, as shown, for example, by the greatly increased radiosensitivity of specific repair-deficient mutants. However, the great variation in radiosensitivity among bacterial species correlates not with initial damage to DNA but rather with the susceptibility of their proteins to radiation-induced oxidation [1], [2]. For example, 90% of Shewanella oneidensis cells, which are hypersensitive to γ-ray-induced protein oxidation, are killed by doses of γ-rays (70 Gy) which cause less than one DSB per haploid genome [3], [4]. In contrast, proteins in extremely radiation resistant bacteria are highly protected from oxidation and the cells can survive hundreds of DSBs caused by ionizing radiation [1], [2], [5].

Remarkably, for bacteria spanning the limits of ionizing radiation resistance [3], [6], for human cells [7], for archaea, yeast, animals and viruses [5], [8], [9], the lesion-yields for DSBs, the most severe form of DNA damage in irradiated cells, are very similar and fall within a narrow range (0.002–0.006 DSB/Gy/Mbp per haploid genome). In contrast, the amount of protein damage in irradiated cells is strongly influenced by their antioxidant status, where yields of radiation-induced protein oxidation can be 100 times greater in sensitive bacteria than in resistant bacteria [5]. We have hypothesized that naturally sensitive bacteria are killed by ionizing radiation mainly owing to the susceptibility of their repair proteins to oxidative inactivation, which could render even minor DNA damage lethal. In contrast, manganese complexes in extremely resistant bacteria may prevent oxidative protein damage, which could protect the activity of enzymes, and thereby greatly increase the efficiency of DNA repair [5]. This exploratory study is the first to examine the nature of radioprotective Mn complexes in D. radiodurans.

In the 1940s, Walter M. Dale demonstrated that enzymes in aqueous solution could be inactivated by small doses of X-rays (10 Gy), mediated by the indirect effects of reactive molecular species derived from the ionization of water [10][12]. The possibility that resistance of cells to ionizing radiation could be increased, specifically by protecting proteins, was supported by studies which showed that the radiosensitivity of an enzyme is not a fixed entity but a variable, where inactivation could be prevented by adding an enzyme's substrate or other small organic compounds [10], [11]. In the 1960s, a low-molecular-weight (<15 kDa), protein-free extract capable of protecting sensitive bacteria against the lethal effects of ionizing radiation was prepared from Deinococcus radiodurans [13], a vegetative bacterium which represents life's utmost limit for ionizing radiation resistance, capable of surviving 12,000 Gy [14][16]. Yet, the active components of the extract and the cellular molecules they protected were not identified [13]. The concordance of this history of results with recent work demonstrating that proteins, but not DNA, in Deinococcus bacteria are extraordinarily resistant to ionizing radiation and desiccation damage [1], [3], [5], [17] led to this study.

Our strategy for elucidating the chemical protective mechanisms utilized by D. radiodurans was to identify inorganic and organic constituents in protein-free cell extracts of D. radiodurans which were over-represented compared to protein-free cell extracts of ionizing radiation-sensitive bacteria. Of the small molecules which were enriched in D. radiodurans protein-free cell extracts, peptides were by far the most abundant. At physiologically relevant concentrations, reconstituted mixtures of peptides, Mn2+ and orthophosphate bestowed extraordinary levels of radiation resistance on purified enzymes but did not significantly protect DNA. Collectively, our findings resolve how, after exposure to huge doses of γ-rays, or to months of desiccation in a desert, D. radiodurans cells retain sufficient protein activity to repair their DNA.


Bacterial ultrafiltrates

The four model bacteria investigated here have been the subjects of extensive bioinformatic and experimental comparative analyses [1], [3], [14], [18]. They are the protagonists of our ‘Death by Protein Damage’ model of ionizing radiation toxicity [5]. For D. radiodurans (DR) and the ionizing radiation-sensitive bacteria Pseudomonas putida (PP), Escherichia coli (EC) and Thermus thermophilus (TT), aqueous-phase extracts of cell homogenates were first subjected to ultracentrifugation, and then to ultrafiltration. Ultracentrifugation removed proteins and other macromolecules, and peptides which were greater than 1 kDa; as a precaution, the ultracentrifuged supernatants were ultrafiltrated to remove any contaminating molecules (>3 kDa) released from the pellets during collection of the supernatants. When PP-, EC- or TT-ultrafiltrates were mixed with proteins purified from E. coli and exposed to γ-radiation, high levels of protein oxidation were detected by Western blot carbonyl analysis (Figure 1A); carbonyl groups are the most widely used marker of severe protein oxidation [19]. In contrast, the DR-ultrafiltrate was extremely protective against ionizing radiation-induced protein carbonylation (Figure 1A). We note here that the effects of bacterial ultrafiltrates on protein oxidation by ionizing radiation in vitro parallel the levels of protein oxidation observed for the corresponding bacterial species irradiated in vivo [1]. We also tested the ability of the four ultrafiltrates to protect the activity of the restriction endonuclease BamHI, which is readily inactivated in aerobic aqueous solutions by reactive oxygen species (ROS) generated by 150 Gy [1]. The DR-ultrafiltrate preserved the activity of BamHI exposed aerobically to 4 kGy, but PP-, EC- or TT-ultrafiltrates did not (Figure 1B). When desiccated from DR-ultrafiltrate, BamHI survived at least 66 days, but when desiccated from PP-, EC- or TT-ultrafiltrates, BamHI activity was lost after six days (Figure 1C). We note here that desiccation dose-response relationships for bacterial protein oxidation and survival observed in vivo coincide with these enzyme activity results [17]. Thus, the DR-ultrafiltrate rendered the ROS-sensitive BamHI highly resistant to ionizing radiation and desiccation in the same range as D. radiodurans survival [3]. Extreme resistance to desiccation, ultraviolet (UV) radiation and ionizing radiation are mechanistically coupled in diverse organisms [5], [8], [15], [17], [20]. However, a chemical basis of resistance to these oxidizing conditions in Mn-accumulating bacteria has not yet been investigated.

Figure 1
In vitro and ex vivo protection by DR-ultrafiltrate.

Consistent with the report of a ‘radioresistant factor’ in protein-free extracts of D. radiodurans [13], the DR-ultrafiltrate was radioprotective of E. coli (Figure 1D). Radioprotection by DR-ultrafiltrate was not limited to bacteria. Protection extended to human lymphoblastoid Jurkat T cells cultured in vitro, where exposure to 16 Gy typically kills greater than 50% of cells within 2 days [21] (Figure 1E and S1). In a concentration-dependent manner, treatment of Jurkat T cells with DR-ultrafiltrate fully preserved their viability up to 16 Gy (Figure 1E and S1), a dose which causes approximately 280 DNA DSBs per haploid genome in human cells [7]. We infer that components of the DR-ultrafiltrate were taken up by E. coli and Jurkat T cells, but this has not been investigated.

Small-molecule profiling of the bacterial ultrafiltrates

The chemical constituents of the DR-ultrafiltrate which were identified and whose concentrations were elevated compared to the PP-, EC- and TT-ultrafiltrates include Mn (Figure 2A), phosphate (Figure 2A), uridine, adenosine and uracil (Figure 2B and Table S1), and amino acids and peptides (Figure 2C and S2). The accumulation of Mn is a hallmark of all Deinococcus bacteria [5], [17], [20], and it is established that D. radiodurans exposed to ionizing radiation or UV radiation produces an intracellular pool of nucleotides which are subsequently converted to nucleosides and then exported [22]. Whereas iron concentrations in D. radiodurans are comparable to ionizing radiation-sensitive bacteria, D. radiodurans exhibits Mn concentrations which are 15–150 times greater than radiosensitive bacteria [3]. Under our growth conditions, the total cellular Mn concentration in D. radiodurans was approximately 200 µM (Table 1), although localized Mn concentrations in D. radiodurans can be significantly higher [1]. Most of the cytosolic Fe (83%) in D. radiodurans was bound to proteins, which were precipitated by trichloroacetic acid (TCA), but most of the cytosolic Mn (72%) was resistant to TCA-precipitation (Table 1). In the aqueous-phase extract of D. radiodurans homogenate, which was used to prepare DR-ultrafiltrate, Mn was bound to small molecules (<3 kDa), which included peptides (Figure 2D). Others have established that Mn2+ ions can form antioxidant complexes with various inorganic and organic compounds. Mn2+ and orthophosphate, which do not significantly scavenge hydroxyl radicals (HO) [1] (Figure S3A), form complexes which catalytically remove superoxide (O2•−) via a disproportionation mechanism [23], [24]; and amino acids and peptides, which scavenge HO very efficiently, form complexes with Mn2+ which catalytically decompose hydrogen peroxide (H2O2) [25].

Figure 2
Composition of the DR-ultrafiltrate.
Table 1
Manganese and iron concentrations in cells and their extractsA.

We previously demonstrated that BamHI is more sensitive to O2•− than HO generated by ionizing radiation; under aerobic conditions, BamHI was inactivated by 150 Gy, but survived 800 Gy under anaerobic conditions [1]. The DR-ultrafiltrate was enriched in adenosine and other nucleosides and bases (Figure 2B and Table S1), which scavenge HO efficiently but do not react with O2•− [26]. Figure 3A (gel 1) shows that BamHI did not survive 2.5 kGy when irradiated aerobically in 3 mM adenosine, but survived 5 kGy when irradiated anaerobically in 3 mM adenosine (Figure 3A, gel 2). Thus, the radioprotective effects of limiting O2•−- and HO-mediated damage on the enzyme's activity were synergistic.

Figure 3
Radioprotection by mixtures of Mn2+, orthophosphate, nucleosides and bases.

Multifactorial mode of enzyme protection under ionizing radiation

Since the DR-ultrafiltrate was enriched in Mn2+, inorganic phosphate, nucleosides and bases (Figure 2A and 2B), we tested the radioprotective properties of their mixtures on BamHI. As most of the Mn in D. radiodurans homogenate was lost during ultracentrifugation (bound to molecules >1 kDa; Table 1), we tested a range of Mn2+ concentrations (0.2–1 mM) which matched total intracellular Mn concentrations reported for D. radiodurans (see below). The orthophosphate concentration we used in vitro was 25 mM, which is physiologically relevant to many cell-types, including eukaryotes [27]. Individually, adenosine, Mn2+, and phosphate buffer did not protect BamHI from 2.5 kGy under aerobic conditions (Figure 3A, gels 1, 3 and 6). Together, Mn2+ and phosphate buffer protected BamHI to 10 kGy (Figure 3A, gel 7); and an equivalent mixture which also contained adenosine or uridine protected BamHI to 17.5 kGy (Figure 3A, gels 8 and 9). The substrate of BamHI is DNA, and to determine whether or not radioprotection extended to other classes of enzymes, we tested purified glutamine synthetase. This dodecameric enzyme plays a central role in intermediary metabolism, and is very sensitive to oxidative inactivation by multiple species of ROS [28]. When glutamine synthetase was irradiated in phosphate buffer alone, or phosphate buffer and uridine, 50% activity survived 0.1 kGy (Figure 4E). When irradiated in Mn2+ and phosphate buffer, 50% glutamine synthetase activity survived 1 kGy (Figure 4E). However, when glutamine synthetase was irradiated in an equivalent mixture of phosphate buffer and uridine which contained Mn2+, 50% activity survived 10 kGy (Figure 4E). The presence of Mn2+ was paramount since Mg2+, Ca2+, Fe2+, Ni2+, Cu2+ and Zn2+ had no protective effect when combined with nucleosides and phosphate buffer (Figure 3A, gel 10, and Figure S3B). In vitro, removal of O2•− by Mn2+ is dependent on a threshold concentration of Mn2+ [1], [23], [24]. When the concentration of Mn2+ in phosphate buffer was lowered from 1 mM to 250 µM, radioprotection of BamHI was lost, but protection was restored by the addition of uridine (Figure 3A, gels 11–15) or analogues containing two carbonyl oxygen groups (C = O) separated by one (N3)H group (Figure S3C), a configuration which forms stable Mn2+ complexes when deprotonated [29]. We note that radioprotection of BamHI by uridine was enhanced by the addition of adenosine when Mn2+ and phosphate buffer were present (Figure 3A, gels 11–15), raising the possibility of a role for adenosine-uridine interactions.

Figure 4
Role of amino acids and peptides in ionizing radiation resistance.

When the most abundant free nucleosides and bases detected in the DR-ultrafiltrate (Table S1) were combined with Mn2+ and phosphate buffer, the mixtures were not as radioprotective of BamHI as the DR-ultrafiltrate (Figure 3A, gels 16–19). Moreover, the capacity of the DR-ultrafiltrate to prevent in vitro ionizing radiation-induced protein damage exceeded mixtures of nucleosides and Mn-phosphate (Figure 3B, Coomassie and Carbonyl). Thus, the DR-ultrafiltrate contains radioprotectants beyond Mn2+, phosphate, nucleosides and bases.

Role of amino acids and peptides in resistance

Stadtman and colleagues discovered and characterized an unexpected property of complexes consisting of Mn2+ and amino acids or peptides, namely their ability to catalytically scavenge ROS [25]. We noted above that DR-ultrafiltrate is highly enriched in amino acids and peptides in comparison to the PP-, EC- or TT-ultrafiltrates (Figure 2C), and chromatographic analysis indicated that Mn was bound to peptides in aqueous-phase extracts of the D. radiodurans homogenates used to prepare DR-ultrafiltrate (Figure 2D). We determined peptide concentrations by comparing the amino acid content with and without acid hydrolysis. The latter detects only free amino acids while the hydrolyzed sample detects the sum of free amino acids and those in peptide linkage. D. radiodurans displays very high intracellular protease activities (Figure 4A), and we considered the possibility that peptides detected in the DR-ultrafiltrate were artifacts generated during processing; indeed, proteins in D. radiodurans homogenates were being degraded when homogenates were prepared in phosphate buffer at 0°C. To prevent ex situ protein degradation, we examined aqueous-phase extracts of D. radiodurans homogenates prepared from cells disrupted in the presence of 20% TCA, which completely inactivates enzymes and precipitates all proteins released upon cell lysis, but does not remove small molecules or their complexes. Based on cells disrupted in the presence of 20% TCA, the total cytosolic concentration of amino acids (free and peptide-derived) in non-irradiated D. radiodurans was 9 mM, of which 22% were in peptide linkage (2 mM) (Figure 4B). For D. radiodurans exposed to 7 kGy, the total cytosolic concentration of amino acids was 24 mM, of which 79% were in peptide linkage (19 mM) (Figure 4B). Exposure of D. radiodurans to 7 kGy increased the cytosolic concentration of amino acids in peptide linkage by approximately 10 times, which we attribute to ionizing radiation-induced proteolysis (Figure 4A). In contrast, the total amino acid concentration of the DR-ultrafiltrate was 53 mM (Figure 2C), of which 97% were in peptide linkage (51 mM). Thus, ex situ protein degradation in homogenates prepared in phosphate buffer was substantial, but the presence of 20% TCA during disruption of non-irradiated D. radiodurans cells reduced the level of peptides by 96%. As the free amino acid and peptide-derived amino acid contents of irradiated or non-irradiated D. radiodurans did not increase when the cells were held in phosphate buffer at 32°C for 30 minutes (Figure 4B); and as the concentrations of peptide-derived amino acids in the TCA-homogenates of D. radiodurans did not change when incubated for one hour at room temperature (data not shown), we are confident that the TCA inactivated any proteases in the extract.

We tested the radioprotective properties of the amino acids Leu, Val and Ala, and a synthetic decapeptide (H-Asp-Glu-His-Gly-Thr-Ala-Val-Met-Leu-Lys-OH) whose composition matched that of some of the most abundant amino acids in the hydrolyzed DR-ultrafiltrate (Figure 2C). In the enzyme radioprotection assays (Figure 4C, D and E), the amino acid and peptide concentrations were in the same range as those in D. radiodurans homogenates prepared in 20% TCA. At 10 mM, Leu or Val were highly radioprotective of BamHI when combined with 1 mM Mn2+ in 25 mM phosphate buffer (Figure 4C). As most peptides in D. radiodurans homogenate were 7 to 22 amino acids in length (Figure 2D), the cytosolic concentration of peptides in irradiated D. radiodurans was approximately 1–3 mM (19 mM/22 to 19 mM/7) (Figure 4B). In comparison, the peptide concentration in DR-ultrafiltrate was between 2–8 mM (Figure 2C). At 3 mM, the decapeptide protected the activity of irradiated BamHI to 10 kGy when combined with 25 mM phosphate buffer (Figure 4D, gel 1); when 1 mM Mn2+ was added to the decapeptide-phosphate buffer mixture, BamHI survived greater than 22.5 kGy (Figure 4D, gels 3–4), which far exceeded radioprotection by Mn2+-phosphate buffer alone (Figure 3A, gel 7). In parallel studies which tested the absolute limits of enzyme radioprotection, a combination of the 3 mM decapeptide in phosphate buffer or bicarbonate buffer (Figure 4E) with 0.25–1 mM Mn2+ preserved 20–50% activity of glutamine synthetase exposed to 50 kGy. In stark contrast, DNA incubated in equivalent mixtures of Mn2+, decapeptide and phosphate buffer was obliterated by 12 kGy (Figure 5); and enzymes irradiated in phosphate buffer alone were inactivated by less than 0.5 kGy (Figure 4E).

Figure 5
The HO-scavenging properties of Mn2+, orthophosphate, leucine and the decapeptide (H-Asp-Glu-His-Gly-Thr-Ala-Val-Met-Leu-Lys-OH).

Radioprotection of cells

We tested an ex vivo radioprotection strategy based on our approach to preserving the activity of irradiated enzymes, by treating E. coli with reagents which protected proteins in vitro. Added individually or in combination, the radioprotective properties of Mn2+, phosphate buffer, uridine and dimethyl sulfoxide (DMSO; a specific HO-scavenger which is not metabolized by E. coli; Figure S4A) [30] were determined using E. coli grown in TGY, an undefined rich medium which contains 200 nM Mn [3] (Figure 6A and S4B). When TGY was supplemented individually with Mn2+, uridine, DMSO or phosphate buffer, the resistance of E. coli exposed to 3 kGy was increased by 0, 10, 50 and 800 times, respectively (Figure 6A and S4B). When these agents were combined at concentrations applied individually, the survival of E. coli exposed to 3 kGy was increased by 10,000 times (Figure 6A). Thus, the radioprotective synergistic effects manifested between HO-scavenging agents and catalytic O2•−-scavenging agents on the survival of acutely irradiated E. coli (Figure 6A) mirrored those observed in vitro for irradiated enzymes (Figure 3A, 4C, 4D, and 4E).

Figure 6
Radioprotection of E. coli.

Wild-type E. coli does not grow and is killed under high-level chronic γ-radiation (Figure 6B) [3]. We show that the same combination of HO- and O2•−-scavenging agents which rendered E. coli resistant to high doses of acute ionizing radiation (Figure 6A), endowed E. coli with the ability to grow under high-level chronic irradiation. Under 42 Gy per hour, E. coli did not grow up on TGY; nor when TGY was supplemented with a mixture of uridine/Mn2+/phosphate buffer (UMnP); nor when TGY was supplemented with DMSO alone (Figure 6B). However, when equivalent mixtures of UMnP and DMSO were combined in TGY plates, wild-type E. coli displayed luxuriant growth under 42 Gy/hour (Figure 6B, 6C and S4C), a dose rate which kills all but the most ionizing radiation-resistant bacteria [3]. As for the radioprotective ex vivo effects of DR-ultrafiltrate (Figure 1D and 1E), we infer that components of the uridine/Mn2+/orthophosphate/DMSO mixtures were taken up by the cells, but this has not been investigated.


In the absence of radiation, eukaryotes and bacteria can survive large numbers of DSBs provided the cells functionally express a complement of DNA repair functions. Yeast can survive hundreds of endogenous DSBs generated during meiosis [31], bacteria can endure dozens of DSBs produced by the transient expression of cloned restriction endonucleases [32], and the rate of DSB production in normal human cells is estimated to be 50 per cell per cell cycle [33]. Yet, doses of ionizing radiation which cause equivalent numbers of DSBs are lethal to most cells. Recently, fresh insight into the reparability of DSBs was gained by comparisons of DNA and protein damage in irradiated bacteria which have very different antioxidant statuses and resistances. For a given dose of ionizing radiation, DSB lesion-yields were very similar, but protein oxidation lesion-yields were quantitatively related to survival [1], [2], [5].

Classical models of radiation toxicity assert that X-rays and γ-rays indiscriminately damage cellular macromolecules, principally by indirect effects mediated by HO [5], [34]. Water is the most abundant chemical found in living cells and the primary ROS which arise during the radiolysis of H2O are HO (H2O→HO+H+ [proton]+eaq [hydrated electron]); H2O2 (2 HO→H2O2); and O2•− anions (O2+eaq→O2•−) [5]. Whereas HO are extremely reactive and short-lived, O2•− and H2O2 are relatively inert and long-lived [5], [35]. This, however, does not imply that HO will display greater toxicity [36]. The most consequential damage by O2•− and H2O2 in cells is site-specific, to proteins which contain exposed iron-sulfur or haem groups [35], to proteins which contain cysteine residues, and to proteins containing cation-binding sites where an iron-catalyzed site-specific oxidation occurs [37].

Compared to most organisms, proteins in D. radiodurans are highly protected from ROS, but lose their resistance when purified from the cells [1]. In contrast, DNA in D. radiodurans is damaged with essentially the same dose dependence as in all prokaryotic and eukaryotic cells examined [3], [5], [8]. Notably, the multifactorial and synergistic modes of protection of irradiated enzymes by the reconstituted components of DR-ultrafiltrate (Figure 3A, 4C, 4D and 4E) were not manifested for purified DNA (Figure 5 and S3A). When orthophosphate (13 mM) (Figure 2A), Mn2+ (200 µM) (Table 1), and peptides (3 mM) (calculated from Figure 4B, see above) were combined in vitro at concentrations approximating those in D. radiodurans, the mixture preserved the activity of BamHI and glutamine synthetase exposed to 17.5 kGy (Figure 4D, gels 3–5; and 4E), but did not significantly protect DNA (Figure 5). 17.5 kGy represents the outer limits of D. radiodurans survival and breaks its 4–8 haploid genomes per cell into 1,000–2,000 DSB fragments [3], [38]. Thus, protein protection mediated by small Mn complexes provides an explanation for the large shoulders in ionizing radiation dose-response curves of D. radiodurans survival which distinguish them from radiosensitive organisms [5], [8].

The key difference between naturally radiosensitive and radioresistant bacteria is that the latter appear to have developed chemical mechanisms for protecting their proteins. Based on whole-genome comparisons, there is a remarkable abundance in D. radiodurans of genes encoding catabolic enzymes including phosphatases, nucleases and proteases, which would be expected to give rise to the sorts of small molecules accumulated in the DR-ultrafiltrate. At this juncture, we believe that the peptide concentrations reported in DR-homogenate prepared in 20% TCA (Figure 4B) are physiologically relevant. However, this may not be the case for nucleosides and bases, which were only quantified in DR-ultrafiltrate (Figure 2B and Table S1). As noted above, it is known that D. radiodurans exposed to ionizing radiation produces an intracellular pool of nucleotides which are subsequently converted to nucleosides [22]. There is also a distinct possibility that other small molecules are accumulated in D. radiodurans which were not identified in this study. What we can say with confidence is that D. radiodurans has presented us with a highly protective chemical strategy against ionizing radiation and other oxidizing agents.

Manganese in D. radiodurans

The first report of Mn accumulation in D. radiodurans was by Leibowitz and colleagues [39], who demonstrated that D. radiodurans contained approximately 100 times more Mn than E. coli when grown in a defined minimal medium (DMM). Using neutron activation analysis (NAA), they showed that D. radiodurans accumulated a total of ~0.29×10−18 mol Mn/cell (~1.8×105 Mn atoms/cell; or ~4 mM Mn, given a cell volume of 6.5×10−2µm3) when grown in DMM containing trace levels of Mn; when DMM was supplemented with 10 µM Mn2+, D. radiodurans accumulated a total of ~1.6×10−18 mol Mn/cell (~1×106 Mn atoms/cell; or ~22 mM Mn) [39]. In 2004, the NAA results were corroborated using inductively coupled plasma mass spectrometry [3]; and other studies showed that when D. radiodurans was incubated in minimal medium containing the radioisotope 54Mn, the cells accumulated approximately 3 mM Mn [3]. Recently, X-ray fluorescence (XRF) microspectroscopy revealed that Mn is distributed throughout D. radiodurans cells grown in TGY, but with regional intracellular Mn concentrations ranging from 0.4 to 3 mM [1]; the D. radiodurans cells studied by XRF, however, were desiccated, yielding higher Mn concentrations than would be present in hydrated cells. In contrast, much of the Fe in D. radiodurans was sequestered outside of the cytosol, in the septa of dividing cells [1]. Based on electron paramagnetic resonance (EPR) spectroscopy [3], [39] and X-ray-absorption near-edge structure (XANES) analyses [1], the dominant form of manganese in D. radiodurans cells is Mn2+, with no significant levels of Mn3+ detected. Using atomic absorption spectroscopy, we showed that D. radiodurans (grown in TGY) contains approximately 200 µM Mn, 72% of which was not bound to proteins in the corresponding homogenate (Table 1). Unfortunately, it was not possible to determine Mn speciation within cell extracts because low-molecular-weight Mn2+ complexes exchange their ligands very rapidly in solution, and standard analytical procedures that disrupt cells likely alter speciation [40].

Role of metabolite accumulation in radiation resistance

The existence of chemical antioxidants in D. radiodurans which are based on common metabolites raises the possibility that a major route to radiation resistance and desiccation resistance is via metabolite regulation [41], where equivalent synergistic processes promoted by the accumulation of Mn2+ and small molecules may be acting similarly in other organisms [5]. D. radiodurans encodes an expanded family of proteases [14] which appear to be activated by irradiation (Figure 4B). At least 10 complete open reading frames with considerable sequence similarity to proteases of Baccilus subtilis have been identified in D. radiodurans [41], which are implicated herein as contributing to the pool of peptides needed to form antioxidant Mn2+ complexes. Moreover, wild-type Deinococcus species display unusual metabolic defects which could facilitate metabolite accumulation [41]. For example, D. radiodurans cannot utilize uridine or adenosine as carbon and energy sources (Figure S4A), which could explain the enrichment of these nucleosides in the DR-ultrafiltrate (Figure 2B). Other examples of metabolic deficiencies in wild-type D. radiodurans which could promote the accumulation of metabolites include: (i) D. radiodurans has a functional tricarboxylic acid (TCA) cycle but is unable to grow on a variety of non-fermentable carbon sources (e.g., α-ketoglutarate, succinate, fumarate or malate) [41]. While such a metabolic configuration limits growth, it could promote the accumulation of TCA cycle products and their derivatives (e.g., amino acids); and (ii) D. radiodurans has lost four genes in the biosynthetic pathway of nicotinamide adenine dinucleotide (NAD), a coenzyme which D. radiodurans requires for growth [41], [42]. The absence of enzymes in D. radiodurans which synthesize NAD could explain the accumulation of NAD precursors (nucleotides and their derivatives) in DR-ultrafiltrate. The metabolic configuration of D. radiodurans and its implications for ionizing radiation resistance have been described previously [41].

We demonstrate that wild-type E. coli can develop high levels of radioresistance based on supplementation alone (Figure 6). Resistance to high-level irradiation in naturally sensitive bacteria previously has only been achieved by serial selection of resistant mutants [43], [44]. Notably, as the mutants became more ionizing radiation-resistant, they progressively lost metabolic functions and displayed heterotrophic nutritional modes which resembled those of Deinococcus species [41]. The benefits of Mn accumulation in cells which are unable to amass small molecules may be limited. By replacing Fe2+ and other divalent cations (e.g., Mg2+ and Cu2+) with Mn2+ as mononuclear cofactors in enzymes, active sites would be protected from oxidative damage [45] but might remain vulnerable to primary ROS at other sites in the absence of proximal ligands which activate Mn2+ (e.g., orthophosphate, bicarbonate, amino acids, peptides and nucleosides) (Figure 3A, 4C, 4D and 4E). Thus, any of a number of mutations which cause the loss of metabolic functions could lead to metabolite accumulation and radiation resistance, provided the cells express Mn transport systems.

As the survival of irradiated enzymes and their hosts rests squarely on preventing both site-specific (O2•− and H2O2) and non-specific (HO) forms of ROS damage, the accumulation of Mn2+ together with certain organic metabolites may represent a widespread strategy for combating oxidative stress. For example, dormant spores of Bacillus species accumulate high levels of both Mn and dipicolinic acid, as well as a large depot of small proteins (~70 amino acid residues in length) [46]; cyanobacteria accumulate Mn2+ and nonreducing disaccharides [47]; and numerous other environmentally robust Mn-rich microorganisms accumulate mycosporin-like amino acids [48]; and in mitochondria and chloroplasts, where organellar Mn budgets appear to exceed the demands of their enzymes [49], [50], Mn might form antioxidant complexes as in radiation resistant bacteria.

Application prospects

Since the 1960s, the goal of exporting the radioprotective processes of D. radiodurans outside of the host cell for practical purposes has eluded researchers [5], [13][16]. E. coli and other bacteria which were previously considered far too sensitive to grow under high-level chronic ionizing radiation now emerge as prospective candidates for bioremediation at highly radioactive sites [51] (Figure 6B and 6C). The finding that the treatment of human Jurkat T cells with DR-ultrafiltrate rescued them from γ-ray exposures (16 Gy) which cause ~560 DSBs per diploid cell [7] (Figure 1E and S1) demonstrates that metabolic interventions at the cellular level may be a powerful approach to fight oxidative stress in animal cells during irradiation or aging [52]. Another tangible application of our approach may be the preparation of ionizing radiation-sterilized whole-bacterial cell, whole-virus, or protein vaccines with only nominal loss in immunogenicity. Our finding that mixtures of Mn2+ and the decapeptide in bicarbonate buffer protected approximately 50% activity of the dodecameric glutamine synthetase (622 kDa) at 50 kGy (Figure 4E), supports that complex quaternary protein structures in aqueous solution can be preserved at doses of ionizing radiation which destroy nucleic acids (Figure 5 and S3A) [3], [15], [16], [39]. Others have shown that bacteria exposed to 8 kGy are able to trigger long-lasting immunity [53]. However, the anticipated levels of ionizing radiation required to inactivate bacteria without any risk of infection would render vaccines with no immunogenicity due to oxidation of their antigenic determinants [53]. Similar drawbacks apply to viruses, which require even greater ionizing radiation doses than bacteria for sterilization. Our approach to protecting proteins could be applied to preparing irradiated vaccines, where the epitopes of cells or viruses treated with Mn-peptide complexes in orthophosphate or bicarbonate buffer would be expected to survive doses of ionizing radiation which irreversibly inactivate their genomes.


Recent studies implicate proteins as critical early targets in irradiated cells [1], [2], [54]. DNA repair-proficient bacteria which are unable to protect their proteins from ionizing radiation-induced ROS succumb to relatively minor DNA damage [1], [4], [5]. While impaired DNA DSB repair provides the best available correlation with radiation-induced cell-killing, protection of proteins in radiation resistant bacteria by Mn-dependent chemical antioxidants generally provides an explanation for extreme resistance without invoking the need for novel repair pathways or unusual forms of DNA packaging [5].

Although Mn2+ is most commonly associated with its role as a catalytic cofactor of proteins [27], the majority of cellular Mn in D. radiodurans appears to exist as small Mn2+ complexes (Figure 2C). Moreover, intracellular Mn2+ speciation within yeast has recently been probed through measurements of 1H and 31P electron-nuclear double resonance (ENDOR) signal intensities [27], which support an important in vivo role for the orthophosphate complex of Mn2+ (Mn2+-Pi) in cellular resistance to oxidative stress. Those studies support that Mn2+-Pi, but not Mn2+-polyphosphate or Mn2+-pyrophosphate, can compensate for the loss of superoxide dismutase enzymes and that Mn2+-Pi truly serves as a cellular antioxidant [27]. In D. radiodurans, the accumulation of Mn2+-Pi and peptides is strongly implicated in protecting the proteome from oxidation. We propose that the great efficiency of DNA repair in D. radiodurans is based on Mn2+-Pi-metabolite complexes, which specifically protect cytosolic proteins against ionizing radiation- and desiccation-induced oxidation and thereby preserve the function of enzymes needed to repair DNA and survive.

A recent mathematical model of radiogenic oxidative stress is consistent with our data and can potentially be generalized to other organisms and lower radiation doses [55]. Our studies indicate that supplementation of bacteria and human cells with mixtures of peptides, nucleosides, Mn2+ and orthophosphate, which are enriched in DR-ultrafiltrate, is a major route to radiation resistance mediated by protein protection. Thus, our findings could come to affect approaches to pre-exposure and post-exposure interventions in diverse settings of oxidative stress management.

Materials and Methods

Bacterial ultrafiltrates

The DR- (ATCC BAA-816), PP- (ATCC 47054), EC- (MG1655) and TT- (ATCC BAA-163) ultrafiltrates (Figure 1A, 1B, 1C, ,2,2, ,3,3, and S2) were prepared from bacteria grown as batch cultures (8×1.5 L each) in TGY medium (1% Bacto Tryptone, 0.5% Bacto Yeast Extract and 0.1% glucose) [1], [3] to an optical density of 0.9 (log-phase) determined at 600 nm. For the large-scale production of DR-ultrafiltrate used in radioprotection studies of E. coli and Jurkat T cells (Figure 1D, 1E and S1), D. radiodurans (ATCC BAA-816) was grown under optimized conditions using a 20-L fermentor [56]. Harvested cells were washed twice in distilled and de-ionized H2O (ddH2O), centrifuged at 2,000×g for 15 min at 4°C, and broken open by passage through a French Press (20,000 lb/in2) as described previously [1]. Upon lysis, crude cell extracts (homogenates) were centrifuged at 12,000×g (1 h, 4°C) yielding an aqueous-phase which contained soluble proteins and small molecules, and a pellet of insoluble cell debris. Using the Coomassie (Bradford) assay (BioRad, Hercules, California, USA), the supernatants were standardized for concentration on a protein-basis [1] (18.6 mg/ml) by dilution with ddH2O. The adjusted supernatants were ultracentrifuged at 190,000×g (69 h, 4°C), which removed molecules greater than 1,000 Da [57]. The ultracentrifuged supernatants were subjected to filtration through 3 kDa Centriplus Centrifugal Filter Devices (YM-3) (Millipore Corporation, Bedford, Massachusetts, USA) at room temperature for 3–4 hours. The ultrafiltrates were then boiled for 40 min, concentrated 5 times in a SpeedVac concentrator (Savant, GMI, Inc., Ramsey, Minnesota, USA) at room temperature, aliquoted, and stored at −80°C.

Assay for oxidized proteins

Proteins purified from an E. coli (MG1655) homogenate were used as the substrate for carbonyl analyses reported in (Figure 1A and and3B).3B). Proteins were prepared from E. coli grown in TGY to OD600 0.9. Cells were broken open by passage through a French Press (20,000 lb/in2), and the soluble protein fraction was recovered from the cell homogenate by centrifugation at 12,000×g (1 h, 4°C) and quantified for concentration using the Bradford assay [1]. The proteins were stored at −80°C as 35 mg/ml stocks in 150 µl aliquots, which were used once and then discarded. Before irradiation, a stock of E. coli proteins was diluted in ddH2O and 5×concentrated DR-, PP-, EC- or TT-ultrafiltrate preparations, yielding mixtures with 4.4 mg/ml of E. coli protein in 1×ultrafiltrate (100%) (Figure 1A). For a given E. coli protein-ultrafiltrate sample (100 µl), irradiations were on ice under aerobic conditions. At the indicated ionizing radiation doses (Figure 1A), 20 µl samples were removed and stored on ice until the time-course of irradiation was completed. The carbonyl groups in the E. coli protein samples were then derivatized to 2,4-dinitrophenylhydrazone by reaction with 2,4-dinitrophenylhydrazine for 15 min in 3% (w/v) sodium dodecyl sulfate [1]. Derivatized samples and size standards were processed for carbonyl analysis as described previously [1].


E. coli protein-ultrafiltrate samples (Figure 1A and and3B)3B) and E. coli protein-nucleoside-phosphate samples (Figure 3B) were irradiated in air on ice with 60Co at 4.2 kGy/h. Irradiations of BamHI (Figure 1B, ,3A,3A, 4C, 4D, S3B and S3C) and glutamine synthetase (Figure 4E) were in air on ice with 60Co at 4.2 kGy/h and 13.3 kGy/h, respectively. Acute irradiations of E. coli cells (Figure 1D, ,6A,6A, and S4B) were in air on ice with 60Co at 3.7 kGy/h. Acute irradiations of Jurkat T cells (Figure 1E and S1) were at room temperature with 60Co at 36 Gy/h. Growth of D. radiodurans and E. coli under high-level chronic irradiation (Figure 6B, 6C, and S4C) was at 42 Gy/h (137Cs).

Assays for enzyme activity and DNA damage

The chemical agents identified in the DR-ultrafiltrate (Figure 2A, 2B, 2C, ,4B,4B, and S2; and Table S1) were reconstituted in vitro using reagents from Sigma Chemical Company (St. Louis, Missouri, USA). The synthetic decapeptide (H-Asp-Glu-His-Gly-Thr-Ala-Val-Met-Leu-Lys-OH) was obtained from Elim Biopharmaceuticals, Inc. Hayward, California, USA, and was authenticated at NHLBI by high performance liquid chromatography-mass spectrometry (HPLC-MS). Dilutions of reagents were made with ddH2O. Post-irradiation activity of BamHI was determined as described previously [1]. Briefly, BamHI (3,000 U/µl) (without BSA) (New England Biolabs, Ipswich, Massachusetts, USA) was diluted in the specified bacterial ultrafiltrate to 0.54 U/µl (Figure 1B) or diluted in the various reagent-mixtures to 3.6 U/µl (Figure 3A, 4C, 4D, S3B, S3C); typically, 200 µl of the BamHI mixtures were irradiated aerobically. For the anaerobic BamHI irradiation (Figure 3A, gel 2), 0.5 ml samples were first purged with ultra-high purity argon (99.999%) and then irradiated in sealed tubes [1]. Following irradiation, 20 µl of each ionizing radiation-treated BamHI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2, and 1 mM dithiothreitol (New England Biolabs). BamHI/μ DNA mixtures were incubated for 1.25 h at 37°C, followed by agarose (0.8%) gel electrophoresis [1] (AGE). Post-desiccation activity of BamHI (Figure 1C) was determined as follows. BamHI (3,000 U/µl) (without BSA) (New England Biolabs) was diluted in the specified bacterial ultrafiltrates (Figure 1C), yielding pre-desiccation samples with 20 U/µl of BamHI in 1×ultrafiltrate (Figure 1C). Pre-desiccation samples (8 µl) were transferred to 0.5 ml Eppendorf tubes, which were placed open in an aerobic desiccation chamber over anhydrous calcium sulfate (W. A. Hammond Drierite Company, Ltd., Xenia, Ohio, USA). The desiccation chamber was hermetically sealed and stored at room temperature. At the indicated times, the dried samples were dissolved in 8 µl 1×DR-ultrafiltrate (Figure 1C) and assayed for residual endonuclease activity using μ-phage DNA and AGE as for irradiated BamHI samples (see above). Glutamine synthetase (GS) (40 µg/ml) purification from E. coli, and GS activity measurements post-irradiation were as described previously [28]; each trial presented in Figure 4E was repeated twice. For DNA damage assays, supercoiled pUC19 DNA (1 µg/µl) (New England Biolabs) was diluted (1/25) in the specified reagent mixtures (Figure 5 and S3A).

Survival assays for E. coli and human Jurkat T cells

Wild-type E. coli (K12, MM1925) [58] (Figure 1D, ,6,6, and S4) or its isogenic mntH (Mn-transport) mutant (MM2115) [58] (Figure 6A) were inoculated into TGY liquid medium supplemented or not with the indicated reagents; note, growth and recovery of MM2115 was in the presence of kanamycin (50 µg/ml). The bacteria were grown at 37°C until a culture reached OD600 0.9 (~5×108 colony forming units (CFUs)/ml). For liquid cultures exposed to acute doses of ionizing radiation (60Co), cell survival was determined by serial dilution and CFU analysis [3], with three biological replicates performed for each trial (Figure 1D, ,6A,6A, and S4B). The ability of bacteria to grow under high-level chronic irradiation was tested on unsupplemented and supplemented TGY medium solidified with 1.5% Bacto agar (Figure 6B, 6C, and S4C). For a given strain, the bacteria (1×107 cells) were seeded as lawns onto 4 cm2 sectors, and incubated for 2 days (Figure 6B). The recA mutant of E. coli (DH10B) and recA mutant of D. radiodurans (rec30) were as described previously [59]. In parallel studies, growth of wild-type E. coli (MM1925) under 42 Gy/h was quantified (Figure 6C), where each trial consisted of a single plate with a 4 cm2 sector inoculated with 1×107 cells. Cells from a sector were harvested and enumerated by CFU assay after 24 h, and a second batch of inoculated plates was enumerated after 48 h (Figure 6C). Note, inoculations of ‘TGY+IR’ sectors (4 cm2) with ~1×108 E. coli (MM1925) cells sporadically gave rise to 1–3 colonies per sector, derived from spontaneous ionizing radiation-resistant mutants within a lawn which did not grow. TGY sectors which gave rise to colonies under ionizing radiation were not enumerated for lawn-growth. Thus, sectors (Figure 6B and 6C) were inoculated with 1×107 cells. Low-frequency spontaneous ionizing radiation resistant E. coli mutants precluded quantification in liquid culture under 42 Gy/h. Human Jurkat T cells (ATCC TIB-152) were inoculated at 1×106 cells/ml and grown in 75 cm2 tissue culture flasks (Costar, Cambridge, Massachusetts, USA) containing RPMI 1640 medium supplemented with 0.03% glutamine, 4.5 g/l glucose, 25 mM HEPES, 10% fetal bovine serum, penicillin (50 µg/ml) and streptomycin (50 µg/ml) (Gibco/BRL, Gaithersburg, Maryland, USA). The cells were incubated at 37°C in a 5% CO2-incubator and fed every 3–4 days. Twenty-four hours before irradiation (60Co), the cells were treated with DR-ultrafiltrate (Figure 1E and S1). For each acute dose of ionizing radiation, the Jurkat T cell trials (2 ml with 1×106 cells/ml) were performed in triplicate in 6-well microtiter plates. After irradiation, the microtiter plates were returned to the 5% CO2-incubator for the specified time (1–3 days), and cell viability (Figure 1E and S1) was determined with trypan blue [60]. For each trial, 50 µl of Jurkat T cells were mixed with 50 µl of 4% trypan blue; 20 µl of the cell suspensions were enumerated for viable cells by hemocytometer counts (Figure 1E and S1).

Composition of bacterial extracts

The chemical composition of the DR-, PP-, EC- and TT-ultrafiltrates (Figure 2A, 2B, 2C, and S2; and Table S1) prepared from cells grown in batch culture (see above) was determined as follows: Mn and Fe on a Perkin Elmer model 4100ZL atomic absorption spectrometer; inorganic phosphate by the malachite green assay [61]; bases, nucleosides and nucleotides by HPLC [62]; and amino acids by pre-column derivatization [63] as implemented by Agilent Technologies [64]. HPLC analysis without acid hydrolysis gave the free amino acid content while analysis following acid hydrolysis gave the free and peptide bound content (Figure 2C and S2). The analyses of cell homogenates which were not ultracentrifuged (Figure 2D and and4B;4B; and Table 1) were on cells grown in a 20-L fermentor [56]. Cells were grown to the stationary phase, quick-frozen in liquid nitrogen, then stored at −80°C. At the time of analysis, the cells were thawed and washed three times with 50 mM phosphate buffer, pH 7.4 at 4°C. To determine the size-distribution of small molecules and Mn in D. radiodurans homogenates (Figure 2D), the cells were resuspended in 50 mM phosphate buffer, pH 7.4 containing a protease inhibitor cocktail (Roche “Complete Solution for Protease Inhibition”, 1 mini-tablet per 10 ml buffer; Roche, Basel, Switzerland). The cells were then broken open by a One Shot cell homogenizer (Constant Systems Limited, Daventry, England, UK) at 40,000 lb/in2. The homogenates were centrifuged at 12,000×g (1 h, 4°C), and the molecular weight distribution of Mn, peptides and proteins in the soluble extracts was determined by gel filtration on a 25×1.7 cm column packed with Bio-Gel P4 (Figure 2D). Fractioned aliquots of the soluble extract were subjected to acid hydrolysis and analyzed as described above; and the Mn content of fractions was determined by atomic absorption spectroscopy. In studies which determined the cytosolic amino acid and peptide concentrations in soluble extracts of D. radiodurans homogenates (Figure 4B), the cells were resuspended (1[ratio]1) in 20% trichloroacetic acid (TCA), disrupted at 40,000 lb/in2 (One Shot Cell Disrupter) and centrifuged at 12,000×g (1 h, 4°C). The supernatants were analyzed for amino acids with or without hydrolysis (Figure 4B; data sets are the averages of 2 independent trials), and also for Mn and Fe concentrations (Perkin Elmer model 4100ZL) (Table 1). Protease activities in cell homogenates of E. coli (MG1655) and D. radiodurans (ATCC BAA-816) (Figure 4A) were determined as follows. E. coli and D. radiodurans were each grown in a 20-L fermentor [56], washed in 50 mM phosphate buffer, pH 7.4, and broken open with a One Shot Cell Disrupter at 40,000 lb/in2 (Constant Systems, Ltd.) as described above, yielding soluble extracts. Quantification of protein in the soluble extracts was by the bicinchoninic acid assay (Thermo Scientific, Pierce Protein Research Products, Rockford Illinois, USA). Proteolytic activity was assayed for 20 min at 40°C using azocasein as substrate [65]. The reaction was stopped by precipitating the protein with an equal volume of 20% TCA, vortexing 5 s, incubating 5 min at room temperature, and then centrifuging 20,000×g (5 min, 4°C). The absorbances were determined at 366 nm, and the peptide release calculated; the assay was repeated 4 times on different aliquots.

Supporting Information

Figure S1

DR-ultrafiltrate preserves the viability of irradiated Jurkat T cells. Jurkat T cells in liquid medium were adjusted to 40% DR-ultrafiltrate 24 h before acute irradiation (16 Gy). The viability of irradiated cells was determined by trypan blue staining after 1, 2 and 3 days. Viability assays were in triplicate, with standard deviations shown.

(0.65 MB EPS)

Figure S2

Free amino acid composition of PP-, EC-, TT- and DR-ultrafiltrates. Strain abbreviations as in Figure 1A. The free amino acid concentration of the DR-ultrafiltrate is 1.4 mM. Note, the sum of free amino acids and those in peptide linkage in the PP-, EC-, TT- and DR-ultrafiltrates after acid hydrolysis is presented in Figure 2C. The total amino acid concentration of the DR-ultrafiltrate is 53 mM (Figure 2C), of which 97% are in peptides.

(0.72 MB EPS)

Figure S3

Mixtures of Mn2+ and orthophosphate, and nucleosides and bases protect irradiated enzymes but not DNA. (A) The HO-scavenging properties of Mn2+, phosphate buffer (PiB, pH 7.4), nucleosides and bases (Ns/Nb) (see Table S1 for Ns/Nb added) were tested. pUC19 DNA damage assays and abbreviations as in Figure 5. (B) Radioprotective properties of divalent metals on BamHI. Assay conditions as in Figure 3A, gel 10. (C) Radioprotective properties of nucleosides, bases and nucleotides on BamHI when Mn2+ is limited. Assay conditions as in Figure 3A, gels 11–15. PiB, phosphate buffer, pH 7.4.

(3.33 MB EPS)

Figure S4

Radioprotection of E. coli. (A) E. coli can grow on nucleosides but D. radiodurans cannot. Strain abbreviations as in Figure 1A. Defined minimal medium (DMM) [3] was supplemented with 1% (128 mM) DMSO, 10 mM fructose, 10 mM uridine, or 10 mM adenosine. Cells were pre-grown in DMM with 22 mM fructose for 2 days and then diluted to OD600 0.1 in the indicated medium. Incubations (PP, 32°C; EC, 37°C; TT, 65°C; DR, 32°C) were in triplicate for 70 h, with standard deviations shown. (B) Survival of wild-type (wt) E. coli (MM1925) exposed to acute ionizing radiation (kGy). Cells were grown in, irradiated in, and recovered on the indicated medium: UMnP contained 3 mM uridine/1 µM Mn2+/13 mM phosphate buffer, pH 7.4; DMSO, dimethyl sulfoxide; PiB, phosphate buffer, pH 7.4. (C) Co-incubation of E. coli and D. radiodurans under high-level chronic ionizing radiation (42 Gy/h). E. coli (MM1925) and D. radiodurans were inoculated onto the plates as concentric rings. Within plate 4, the trefoil pattern (red stripes) corresponds to the location of 3 agar segments which were excised and exchanged with the corresponding agar segments from plate 1 immediately after inoculation. Plate 1 (control) was incubated for 3 days at 25°C without ionizing radiation (IR). Plates 2–4 were incubated for 3 days at 25°C under 42 Gy/h. Abbreviations as in panel B. Color key: green, E. coli (EC); orange, D. radiodurans (DR). Since growth of D. radiodurans on TGY under 42 Gy/h is inhibited by 3% DMSO (Figure 6B), this approach (panel C) allowed us to demonstrate growth of D. radiodurans (red-pigmented) and E. coli (non-pigmented) on the same plate under 42 Gy/h.

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Table S1

Concentration of nucleosides (Ns), bases (Nb) and nucleotides (Nt) in the bacterial ultrafiltrates (100%).

(0.05 MB DOC)


We thank Yi He (National Institutes of Health, Bethesda, MD) for providing large-scale preparations of D. radiodurans.


Competing Interests: The authors have declared that no competing interests exist.

Funding: The work of E.K.G., V.Y.M. and M.J.D. was supported by the Air Force Office of Scientific Research (Grant FA9550-07-1-0218). The work of B.S.B., G.A.V., N.B.W., D.-Y.L. and R.L.L. was supported by the Intramural Research Program of the National Heart, Lung, and Blood Institute, National Institutes of Health. The work of J.G.K. and R.F. was supported by the National Institute of Allergy and Infectious Diseases, and the Intramural Program of the AFRRI. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


1. Daly MJ, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, et al. Protein oxidation implicated as the primary determinant of bacterial radioresistance. PLoS Biol. 2007;5:e92. [PMC free article] [PubMed]
2. Kriško A, Radman M. Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans. Proc Natl Acad Sci USA. 2010 ( www.pnas.org/cgi/doi/10.1073/pnas.1009312107) [PMC free article] [PubMed]
3. Daly MJ, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, et al. Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance. Science. 2004;306:1025–1028. [PubMed]
4. Qiu X, Daly MJ, Vasilenko A, Omelchenko MV, Gaidamakova EK, et al. Transcriptome analysis applied to survival of Shewanella oneidensis MR-1 exposed to ionizing radiation. J Bacteriol. 2006;188:1199–1204. [PMC free article] [PubMed]
5. Daly MJ. A new perspective on radiation resistance based on Deinococcus radiodurans. Nat Rev Microbiol. 2009;7:237–245. [PubMed]
6. Ulmer KM, Gomez RF, Sinskey JA. Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: sedimentation properties of irradiated nucleoids and chromosomal deoxyribonucleic acid. J Bacteriol. 1979;138:475–485. [PMC free article] [PubMed]
7. Rothkamm K, Löbrich M. Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses. Proc Natl Acad Sci USA. 2003;100:5057–5062. [PMC free article] [PubMed]
8. Gladyshev E, Meselson M. Extreme resistance of bdelloid rotifers to ionizing radiation. Proc Natl Acad Sci USA. 2008;105:5139–5144. [PMC free article] [PubMed]
9. Krisch RE, Flick MB, Trumbore CN. Radiation chemical mechanisms of single- and double-strand break formation in irradiated SV40 DNA. Radiat Res. 1991;126:251–259. [PubMed]
10. Dale WM. The effect of X-rays on enzymes. Biochem J. 1940;34:1367–1373. [PMC free article] [PubMed]
11. Dale WM. The effect of X-rays on the conjugated protein d-amino-acid oxidase. Biochem J. 1942;36:80–85. [PMC free article] [PubMed]
12. Dale WM. Effect of X-rays on aqueous solutions of biologically active compounds. Brit J Radiol. 1943;16:171–172.
13. Bruce AK. Extraction of the radioresistant factor of Micrococcus radiodurans. Radiat Res. 1964;22:155–164. [PubMed]
14. Makarova KS, Aravind L, Wolf YI, Tatusov RL, Minton KW, et al. Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol Rev. 2001;65:44–79. [PMC free article] [PubMed]
15. Cox MM, Battista JR. Deinococcus radiodurans - the consummate survivor. Nat Rev Microbiol. 2005;3:882–892. [PubMed]
16. Slade D, Lindner AB, Paul G, Radman M. Recombination and replication in DNA repair of heavily irradiated Deinococcus radiodurans. Cell. 2009;136:1044–1055. [PubMed]
17. Fredrickson JK, Li SM, Gaidamakova EK, Matrosova VY, Zhai M, et al. Protein oxidation: key to bacterial desiccation resistance? ISME J. 2008;2:393–403. [PubMed]
18. Omelchenko MV, Wolf YI, Gaidamakova EK, Matrosova VY, Vasilenko A, et al. Comparative genomics of Thermus thermophilus and Deinococcus radiodurans: divergent routes of adaptation to thermophily and radiation resistance. BMC Evol Biol. 2005;5:57. [PMC free article] [PubMed]
19. Requena JR, Levine RL, Stadtman ER. Recent advances in the analysis of oxidized proteins. Amino Acids. 2003;25:221–226. [PubMed]
20. Shashidhar R, Kumar SA, Misra HS, Bandekar JR. Evaluation of the role of enzymatic and nonenzymatic antioxidant systems in the radiation resistance of Deinococcus. Can J Microbiol. 2010;56:195–201. [PubMed]
21. Syljuasen RG, Hong JH, McBride WH. Apoptosis and delayed expression of c-jun and c-fos after gamma irradiation of Jurkat T cells. Radiat Res. 1996;146:276–282. [PubMed]
22. Battista JR. Against all odds: the survival strategies of Deinococcus radiodurans. Annu Rev Microbiol. 1997;51:203–224. [PubMed]
23. Barnese K, Gralla EB, Cabelli DE, Valentine JS. Manganous phosphate acts as a superoxide dismutase. J Am Chem Soc. 2008;130:4604–4606. [PubMed]
24. Archibald FS, Fridovich I. The scavenging of superoxide radical by manganous complexes: in vitro. Arch Biochem Biophys. 1982;214:452–463. [PubMed]
25. Berlett BS, Chock PB, Yim MB, Stadtman ER. Manganese(II) catalyzes the bicarbonate-dependent oxidation of amino acids by hydrogen peroxide and the amino acid-facilitated dismutation of hydrogen peroxide. Proc Natl Acad Sci USA. 1990;87:389–393. [PMC free article] [PubMed]
26. von Frijtag JK, Kunzel D, van der Zee J, Ijzerman AP. Radical scavenging properties of adenosine and derivatives in vitro. Drug Develop Res. 1996;37:48–54.
27. McNaughton RL, Reddi AR, Clement MHS, Sharma A, Barnese K, et al. Probing in vivo Mn2+ speciation and oxidative stress resistance in yeast cells with electron-nuclear double resonance spectroscopy. Proc Natl Acad Sci USA. 2010 ( www.pnas.org/cgi/doi/10.1073/pnas.1009648107) [PMC free article] [PubMed]
28. Stadtman ER. The story of glutamine synthetase regulation. J Biol Chem. 2001;276:44357–44364. [PubMed]
29. Knobloch B, Linert W, Sigel H. Metal ion-binding properties of (N3)-deprotonated uridine, thymidine, and related pyrimidine nucleosides in aqueous solution. Proc Natl Acad Sci USA. 2005;102:7459–7464. [PMC free article] [PubMed]
30. Repine JE, Pfenninger OW, Talmage DW, Berger EM, Pettijohn DE. Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical. Proc Natl Acad Sci USA. 1981;78:1001–1003. [PMC free article] [PubMed]
31. Burgoyne PS, Mahadevaiah SK, Turner JMA. The management of DNA double-strand breaks in mitotic G2, and in mammalian meiosis viewed from a mitotic G2 perspective. BioEssays. 2007;29:974–986. [PubMed]
32. Heitman J, Zinder ND, Model P. Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. Proc Natl Acad Sci USA. 1989;86:2281–2285. [PMC free article] [PubMed]
33. Vilenchik MM, Knudson AG. Radiation dose-rate effects, endogenous DNA damage, and signaling resonance. Proc Natl Acad Sci USA. 2006;103:17874–17879. [PMC free article] [PubMed]
34. Blok J, Loman H. The effects of μ-radiation in DNA. Curr Top Radiat Res Q. 1973;9:165–245. [PubMed]
35. Imlay JA. Cellular defenses against superoxide and hydrogen peroxide. Annu Rev Microbiol. 2008;77:755–776. [PMC free article] [PubMed]
36. Omar BA, Flores SC, McCord JM. Superoxide dismutase: pharmacological development and applications. In: August JT, Anders MW, Murad F, editors. Advances in Pharmacology. San Diego, USA: Academic Press, Inc.; 1992. pp. 109–161. [PubMed]
37. Stadtman ER, Levine RL. Chemical modifications of proteins by reactive oxygen species. In: Dalle-Donne I, Scaloni A, Butterfield DA, editors. Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases. New Jersey, USA: John Wiley & Sons, Inc; 2006. pp. 3–24.
38. Lin J, Qi R, Aston C, Jing J, Anantharaman TS, et al. Whole genome shotgun optical mapping of Deinococcus radiodurans using genomic DNA molecules. Science. 1999;285:1558–1561. [PubMed]
39. Leibowitz PJ, Schwartzberg LS, Bruce AK. The in vivo association of manganese with the chromosome of Micrococcus radiodurans. Photochem Photobiol. 1976;23:45–50. [PubMed]
40. Wang S, Westmoreland TD. Correlation of relaxivity with coordination number in six-, seven-, and eight-coordinate Mn(II) complexes of pendant-arm cyclen derivatives. Inorg Chem. 2009;48:719–727. [PMC free article] [PubMed]
41. Ghosal D, Omelchenko MV, Gaidamakova EK, Matrosova VY, Vasilenko A, et al. How radiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiol Rev. 2005;29:361–375. [PubMed]
42. Brim H, Venkateswaran A, Kostandarithes HM, Fredrickson JK, Daly MJ. Genetic development of Deinococcus geothermalis for bioremediation of high temperature radioactive waste environments. Appl Environ Microbiol. 2003;69:4575–4582. [PMC free article] [PubMed]
43. Davies R, Sinskey AJ. Radiation-resistant mutants of Salmonella typhimurium LT2: development and characterization. J Bacteriol. 1973;113:133–144. [PMC free article] [PubMed]
44. Parisi A, Antoine AD. Increased radiation resistance of vegetative Bacillus pumilus. App Microbiol. 1974;28:41–46. [PMC free article] [PubMed]
45. Anjem A, Varghese S, Imlay JA. Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli. Mol Microbiol. 2009;72:844–858. [PMC free article] [PubMed]
46. Setlow B, Atluri S, Kitchel R, Koziol-Dube K, Setlow P. Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins. J Bacteriol. 2006;188:3740–3740. [PMC free article] [PubMed]
47. Shirkey B, McMaster NJ, Smith SC, Wright DJ, Rodriguez H, et al. Genomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long-term (decades) desiccation but is protected from oxidative damage and degradation. Nucleic Acids Res. 2003;31:2995–3005. [PMC free article] [PubMed]
48. Oren A, Gunde-Cimerman N. Mycosporines and mycosporine-like amino acids: UV protectants or multipurpose secondary metabolites. FEMS Microbiol Lett. 2007;269:1–10. [PubMed]
49. Gunter TE, Gavin CE, Gunter KK. The case for manganese interaction with mitochondria. Neurotoxicology. 2009;30:727–729. [PMC free article] [PubMed]
50. Wolfe-Simon F, Starovoytov V, Reinfelder JR, Schofield O, Falkowski PG. Localization and role of manganese superoxide dismutase in a marine diatom. Plant Physiol. 2006;142:1701–1709. [PMC free article] [PubMed]
51. Brim H, McFarlan SC, Fredrickson JK, Minton KW, Zhai M, et al. Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments. Nat Biotechnol. 2000;18:85–90. [PubMed]
52. Stadtman ER. Protein oxidation and aging. Free Radic Res. 2006;40:1250–1258. [PubMed]
53. Datta SK, Okamoto S, Hayashi T, Shin SS, Mihajlov I, et al. Vaccination with irradiated Listeria induces protective T cell immunity. Immunity. 2006;25:143–152. [PubMed]
54. Bosshard F, Riedel K, Schneider T, Geiser C, Bucheli M, et al. Protein oxidation and aggregation in UVA-irradiated Escherichia coli cells as signs of accelerated cellular senescence. Environ Microbiol. 2010 doi: 10.1111/j.1462-2920.2010.02268.x. [PubMed]
55. Shuryak I, Brenner DJ. A model of interactions between radiation-induced oxidative stress, protein and DNA damage in Deinococcus radiodurans. J Theor Biol. 2009;261:305–317. [PMC free article] [PubMed]
56. He Y. High cell density production of Deinococcus radiodurans under optimized conditions. J Ind Microbiol Biotechnol. 2009;36:539–546. [PubMed]
57. Schachman HK. Ultracentrifugation in Biochemistry. New York: Academic Press; 1959.
58. Kehres DG, Zaharik ML, Brett BF, Maguire ME. The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen. Mol Microbiol. 2000;36:1085–1100. [PubMed]
59. Daly MJ, Minton KW. An alternative pathway of recombination of chromosomal fragments precedes recA-dependent recombination in the radioresistant bacterium Deinococcus radiodurans. J Bacteriol. 1996;178:4461–4471. [PMC free article] [PubMed]
60. Kiang JG, Smith JT, Agravante NG. Geldanamycin analog 17-DMAG inhibits iNOS and caspases in gamma-irradiated human T cells. Radiat Res. 2009;172:321–330. [PubMed]
61. Itaya K, Ui M. A new micromethod for the colorimetric determination of inorganic phosphate. Clin Chim Acta. 1966;14:361–366. [PubMed]
62. Lazzarino G, Di Pierro D, Tavazzi B, Cerroni L, Giardina B. Simultaneous separation of malondialdehyde, ascorbic acid, and adenine nucleotide derivatives from biological samples by ion-pairing high-performance liquid chromatography. Anal Biochem. 1991;197:191–196. [PubMed]
63. Jones BN, Gilligan JP. o-Phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids. J Chromatogr. 1983;266:471–482. [PubMed]
64. Woodward C, Henderson JW, Wielgos T. High-speed amino acid analysis (AAA) on 1.8 µm reversed-phase (RP) columns. Agilent Technologies Application Note. 2007;5989-6297EN
65. Bergmeyer HU. Methods of Enzymatic Analysis 2nd Ed. 1974. pp. 1072–1074. (Orlando, FL, Academic Press) Vol 2.

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