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Amino acid starvation in the absence of DksA increases localization of RecA. Control and ΔdksA cells expressing the RecA-GFP fusion protein were treated with SHX as in Fig. 2A, C, and samples were taken for microscopy after 40 min. (A) The percentage of cells with at least one RecA-GFP focus. (B) The number of RecA-GFP foci per cell length (μm). (C to F) Micrographs of dksA+ (C, E) and ΔdksA (D, F) cells during normal growth (C, D) and after SHX-treatment (E, F). In (C), the nucleoid-associated RecA-GFP are indicated with white arrows. Scale bar: 5μm.

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