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Curr Opin Cell Biol. Author manuscript; available in PMC Jun 1, 2011.
Published in final edited form as:
PMCID: PMC2900470
NIHMSID: NIHMS186874

Multiple faces of the SAGA complex

Summary of Recent Advances

The SAGA complex provides a paradigm for multi-subunit histone modifying complexes. Although first characterized as a histone acetyltransferase, due to the Gcn5 subunit, SAGA is now known to contain a second activity, a histone deubiqutinase, as well as subunits important for interactions with transcriptional activators and the general transcription machinery. The functions of SAGA in transcriptional activation are well-established in S. cerevisiae. Recent studies in S. pombe, Drosophila, and mammalian systems reveal that SAGA also has important roles in transcript elongation, the regulation of protein stability, and telomere maintenance. These functions are essential for normal embryo development in flies and mice, and mutations or altered expression of SAGA subunits correlate with neurological disease and aggressive cancers in humans.

Introduction

Histone modifications, such as acetylation and ubiquitination, play a key role in facilitating a number of cellular events, including gene regulation. Acetylation of histones is largely associated with relaxing chromatin structure to support the entry of transcriptional machinery to genomic loci for activation, while histone ubiquitination has been linked to both gene activation and repression [1, 2]. The histone modifying enzymes that catalyze these post-translational modifications are often integrated into large multi-subunit complexes to facilitate their enzymatic activity and substrate specificity. SAGA (Spt-Ada-Gcn5 acetyltransferase) is a 2MDa multi-protein chromatin modifying complex that is conserved between yeast and humans and harbors two known enzymatic modules that mediate the acetylation and deubiquitination of histones as well as non-histone substrates [3-5]. However, like many multi-subunit complexes, the functions of several components in the SAGA complex are still unknown. In addition, it is unclear exactly how the members of SAGA interact, coordinate and regulate the acetyltransferase and deubiquitinase activites of the complex, and whether the acetyltransferase and deubiquitinase (Dub) modules function in a cooperative or antagonistic manner to regulate cellular processes, such as gene regulation. Here we will discuss the most recent advances made toward deciphering how the catalytic activities of the SAGA complex function in development and disease.

The SAGA complex

The Tetrahymena thermophila protein, p55, was the first identified transcription related histone acetyltransferase (HAT) enzyme and is the ortholog of the yeast (y) transcriptional co-activator protein, Gcn5 (general control nonderepressible 5) [1]. Recombinant yGcn5 acetylates non-nucleosomal histone 3 (H3) lysine residue 14 (K14) [6, 7]. However, the incorporation of yGcn5 into native multi-subunit complexes, such as ySAGA, expands its specificity to additional lysines in nucleosomal histones in vitro, including H3 K9, 14, 18, 23 and to a lesser degree histone H2B [7, 8].

The ySAGA complex is modular in structure and has distinct functional units (Table 1) including a recruitment module (Tra1) [9, 10]; acetylation module (Gcn5, Ada2, Ada3) [11-14]; TBP interaction unit (Spt3, Spt8) [13, 15, 16]; Dub module (Ubp8, Sus1, Sgf11 and Sgf73) [17-21]; and architecture unit (Spt7, Spt20, Ada1, TAF5, -6, -9 and -12) [8, 13, 22, 23]. ySAGA is recruited to gene loci by the interaction of Tra1 with specific transcriptional activators [10], and the bromodomain of yGcn5 binds acetylated H3 and H4 N-terminal tails which potentiates cooperative nucleosome acetylation of histone H3 [24, 25] (Figure 1A). The HAT module composed of yGcn5, yAda2 and yAda3 catalyzes this acetylation, opening up the chromatin landscape for binding of additional transcription factors and the pre-initiation complex (PIC) [14]. The ySpt3 subunit of ySAGA also recruits TBP to facilitate PIC formation and transcriptional activation (Figure 1B) [16]. In addition to promoting gene activation, a modified ySAGA complex, lacking ySpt8, accompanies Pol II during elongation and functions to acetylate and subsequently evict nucleosomes from gene coding regions (Figure 1 C, D) [26]. The Dub module of ySAGA, including Ubp8, Sus1 and Sgf11, also facilitates elongation through deubiquitination of H2B K123ub1 (Figure 1C), which allows for the recruitment of the Ctk1 kinase and subsequent Ser2 phosophorylation of the Pol II C-terminal domain (Figure 1D) [27]. ySAGA contributes to other aspects of gene expression as well. For example, SAGA-mediated histone acetylation functions in co-transcriptional spliceosome assembly and the recruitment of U2 snRNP to intron branchpoints [28], and SAGA-associated Sgf73 and Sus1 proteins link histone deubiquitination to the mRNA export machinery [29].

Figure 1
The enzymatic activities of SAGA facilitate transcription. Different modules of SAGA are required for various stages of gene activation. A. The recruitment module localizes the SAGA complex to target genes, via an activator protein, allowing the HAT module ...
Table 1
Components of the SAGA multi-subunit complexes in yeast, Drosophila and human. The protein structures of the SAGA orthologs are shown to scale. The legend denotes the domain names. The protein domains were determined with SMART (http://smart.embl-heidelberg.de/ ...

The components, modules and functions of the ySAGA are highly conserved across many species and act as a model for understanding the Drosophila (d) and human (h) SAGA complexes. The remainder of our discussion will focus on the enzymatic modules of these metazoan SAGA complexes and how their regulation is intimately linked to both development and disease.

The SAGA histone acetyltransferase module

Although the general compositions of dSAGA and hSAGA complexes are very similar to the ySAGA complex, some differences have been observed. The GCN5 transcript is alternatively spliced in mouse and human cells, generating a short (GCN5S) isoform similar to yGcn5, and more predominantly expressed long (GCN5L) protein isoform that is incorporated into hSAGA and is highly analogous to the GCN5-related family member, PCAF [30, 31]. This long GCN5 isoform is also conserved in Drosophila Gcn5 (dGcn5), suggesting an evolutionary gain of function in ancestral metazoans [32]. GCN5L (hereafter referred to as GCN5) contains both a HAT domain and a bromodomain similar to yGcn5, but also has an extended N-terminal domain which confers the ability to acetylate mononucleosomal H3 as well as free H3 in vitro [30, 33]. Metazoans also have two proteins similar to yAda2, ADA2A and ADA2B, which are found in distinct GCN5-containing complexes [33-36]. In particular, ADA2B is present in hSAGA and serves to bridge the interactions between GCN5 and ADA3 to facilitate acetylation of chromatin substrates [33]. Interestingly, both ADA2B and ADA3 may regulate distinct aspects of chromatin acetylation, as ADA2B-GCN5 dimeric complexes enhance the ability of GCN5 to acetylate mononucleosomes, whereas acetylation of polynucleosomes is strictly mediated by ADA2B-GCN5-ADA3 heterotrimeric complexes in vitro [33]. dAda2b and dAda3 also likely regulate some aspect of chromatin acetylation in vivo, as deletion of either dAda2b or dAda3 leads to decreased H3 K9, 14 acetylation levels on polytene chromosomes [37, 38]. Furthermore, loss of Wda (a yTAF5 homolog) in Drosophila also reduces H3 K9 acetylation levels without compromising the integrity of the dSAGA complex, suggesting a role for Wda in chromatin acetylation [39]. Post-translational modifications identified on these and other subunits [40], as well as the acetylation of hADA3 by GCN5 [33], imply that PTMs may also play an important but unexplored role in regulating the functions of SAGA, including HAT activity.

As in yeast, hSAGA is recruited to specific target genes by activators, such as c-Myc, p53 and E2F, to stimulate the acetylation of histones and the activation of transcription [41-45].

While gene expression profiling for GCN5 has yet to be reported in mammalian cells, experiments in both budding (S. cerevisiae) and fission (S. pombe) yeast support the idea that GCN5 is a gene-specific co-activator, as yGcn5 only appears to regulate 4% and 1.1% of genes, respectively [46, 47]. However, numerous genome–wide mapping studies of histone acetylation patterns demonstrate that yGcn5 globally acetylates H3 K9, 14 and that Gcn5 binds within the promoter and 5’ coding regions of most highly transcribed genes in S. cerevisiae [48-52]. Additionally, genome-wide analysis of yGcn5 in S. pombe indicates that Gcn5 may have a general role in elongation, given that yGcn5 is found within the coding region of actively transcribed genes, and correlates with high H3 K14 acetylation levels [53]. Interestingly, hSAGA contains at least three subunits that have the ability to bind histone tails, including SGF29 which harbors a chromodomain, hSTAF65γ that displays a BTP domain (bromodomain transcription factors and PHD containing), and hADA2B that has a SANT domain (DNA binding domain in the SWI-SNF and ADA complexes, the transcriptional co-repressor N-CoR and TFIIIB). A role for these subunits in SAGA recruitment has not been reported; however, global Gcn5 mediated acetylation may be modulated by such domains. Collectively, these studies indicate that hSAGA functions both in gene specific and in global transcription events and that it can act at multiple steps in the transcription cycle.

The SAGA Deubiquitination module

Although first discovered and characterized as a HAT complex, SAGA was later discovered to harbor a second catalytic module centered on the Ubp8 (yeast) or USP22 (metazoan) deubiquitinases. As with GCN5, the Dub subunit may work most efficiently in the context of a module within SAGA that contains additional components [29]. In yeast, Sus1, Sgf11, and Ubp8 form a structural entity within the ySAGA holoenzyme, and the association of this Dub module with the rest of the complex is mediated by Sgf73 [29]. Sgf11 does not have a substantial role in maintaining the overall composition or integrity of SAGA but appears to mediate either recruitment or stable association of Ubp8 in ySAGA [20, 54]. Sus1 is present in two functionally different protein complexes in both yeast and in flies, the SAGA complex where it regulates its deubiquitination activity through interactions with Ubp8 and Sgf11 and the TREX/AMEX complex, where it plays a role in mRNA export [55-57]. Such dual functions of E(y) 2 (the Drosophila ortholog of Sus1) have implicated dSAGA in the positioning of specific gene loci (like hsp70) to the nuclear periphery, achieving a tightly regulated link between transcription and mRNA export under stress conditions [56]. hUSP22 deubiquitinates H2Bub, like the yeast ortholog, but can also deubiquitinate H2A in vitro , implicating its function in the regulation of Polycomb-mediated H2A ubiquitination [58, 59].

Polyglutamine (polyQ) expansions in the N-terminal region of ATXN7 (the mammalian ortholog of Sgf73) are associated with spinocerebellar ataxia type 7 (SCA7), which is characterized both by motor coordination deficiencies (ataxia) and retinal defects. Mouse models of SCA7 indicate that both the wild type and mutant forms of ATXN7 integrate into the SAGA complex and do not affect SAGA composition. However the effects of polyQ ATXN7 on SAGA HAT activity or on SAGA-mediated gene activation are not clear. In one set of experiments, the acetyltransferase activity of the complex did not appear to be affected by transgenic polyQ ATXN7, even though chromatin immunoprecipitation experiments indicated aberrant SAGA recruitment and hyperacetylation of H3 K9, 14 on rod cell-specific gene promoters in the retinas of the transgenic mice. Surprisingly these hyperacetylation events correlated with decreased mRNA levels of the analyzed genes [60]. In contrast, two other studies which used either cultured cells or yeast expressing the polyQ forms of ATXN7/Sgf73 came to very different conclusions on the effects of the mutant protein on SAGA functions [61, 62]. Reduced levels of acetylated H3 were observed on promoter-enhancer regions of photoreceptor genes, likely caused by impaired acetyltransferase activity of the complex in the presence of the polyQ expanded form of ATXN7 [62]. Neither of these studies addressed the effects of the polyQ allele on the Dub module. Clearly, much more work is needed to understand how the polyQ expansion affects SAGA recruitment and enzymatic activities.

The role of SAGA in development

Given that SAGA is conserved across many species and plays an essential role in regulating gene expression, it is perhaps not surprising that particular subunits of the SAGA HAT module control developmental processes. In Drosophila, loss of dGcn5 is lethal due to a lack of metamorphosis[63]. Furthermore, dAda2b and dAda3, which regulate H3 K9, 14 acetyaltion are also essential for Drosophila viability. In a similar manner, Gcn5 is required for normal development in mice. Gcn5 null (Gcn5-/-) mice are embryonic lethal at 10.5 days post coitum (d.p.c.) and do not acquire normal mesoderm due to enhanced apoptosis in these tissues [64]. However, catalytically inactive Gcn5 mice survive slightly longer (16.5 d.p.c.) and display both neural tube closure defects and exencephaly, implying that Gcn5 HAT activity is required for proper development and that SAGA also has HAT-independent functions [65]. Interestingly, PCAF null mice develop normally, but double homozygous null Gcn5 and PCAF embryos die at 7.5 d.p.c. indicating a functional redundancy for these two HATs during embryonic development [64, 66]. In cell culture, Gcn5 -/- mouse embryonic stem cells remain pluripotent and are capable of differentiation into all three germ layers [67]. The role of GCN5 in ES cells has yet to be defined, but is of considerable interest. H3 K56ac is linked to pluripotency of human embryonic stem cells and GCN5 acetylates H3 K56 on free histone in vitro, suggesting that GCN5 may play a role in maintaining stemness [68, 69].

In addition to the HAT module regulating larval and embryonic development, the SAGA Dub module has been identified to control different aspects of neural development. This is observed by mutations in the Drosophila nonstop (USP22 ortholog) and dSgf11 proteins leading to defects in axonal targeting photoreceptors [70, 71]. Furthermore, retinal defects are also observed in the SCA7 neurodegenerative disease as discussed above, but the roles of USP22 or other Dub module subunits in development have not yet been defined.

The role of SAGA in cancer

The role of hSAGA in cancer is a good paradigm of its multi-functionality. For example, TRRAP and GCN5 regulate the oncogenic activity of the c-Myc oncoprotein and the p53 tumor suppressor [41]. Additional studies underscore that the whole SAGA complex may function in tumorigenesis. STAF65γ (hSPT7) has a role in the transcription of several Myc-dependent genes [43]. Furthermore, SAGA recruitment may require SGF29, a poorly studied subunit of the complex, whose deregulated expression is implicated in malignant transformation [72]. In parallel, GCN5/PCAF acetylates non–histone proteins involved in transcriptional activation or repression, such as c-Myc [73].

The deubiquitination activity of the hSAGA complex has also been implicated in cancer, as USP22 has been grouped by transcriptional profiling into an 11-gene “signature” of poor prognosis in multiple types of cancer [59, 74]. This signature also includes the PRC1 polycomb repressor complex subunit, Bmi1, a known regulator of self renewal in normal and malignant stem cells, suggesting USP22 may be a marker for cancer stem cells. Specifically, USP22 may be required for cell-cycle progression and could function with GCN5 to control certain cell cycle genes. GCN5 has been implicated in the transcriptional regulation of cyclin A, cyclin D3, PCNA and cdc25b, as well as CDK1 and cyclin B1 after DNA damage [75]. Furthermore, GCN5 acetylates the non-histone protein CDC6 to promote S-phase progression of the cell cycle. GCN5 and USP22 also protect telomeres from DNA damage response through the stabilization of a shelterin component called TRF1, and interestingly, this regulation is not transcriptional but involves USP22-mediated deubiquitination of TRF1 [76]. In addition, the Gcn5 related protein, PCAF, appears to regulate ubiquitination of Hdm2 through an intrinsic ubiquitination activity [77] and chk2 turnover [78], adding another level of regulation to an already complicated network.

Conclusions

SAGA is comprised of distinct modules that intimately collaborate to maintain structural integrity, genomic recruitment and interactions with the basal transcription machinery to localize the HAT and Dub activities of the complex to gene loci. Elegantly, SAGA mediates histone acetylation of gene promoters to enhance transcriptional activation and facilitates elongation by deubiquitinating histones downstream of Pol II and acetylating histones within the coding region to promote histone eviction (Figure 1). Functional HAT and Dub modules of the SAGA complex are central to normal mouse and Drosophila development. In addition, SAGA functions in cancer, as USP22 is part of an 11 gene “signature” associated with poor cancer prognosis and SAGA contributes to the activation of c-Myc responsive genes. However, many questions remain unanswered regarding the intricate regulation of this co-activator complex. Do SAGA components outside of the HAT and Dub modules either stimulate or suppress these enzymatic activities (Figure 1A)? What role do posttranslational modifications of the SAGA components play in complex regulation (Figure 1B)? Is SAGA-mediated histone acetylation required for Dub activity downstream of Pol II (Figure 1C), and do members of the HAT and Dub modules cross regulate each other (Figure 1C, D)? In addition to these questions, much remains to be elucidated about the biological function of SAGA. Little is known about the role of USP22 in mammalian development or the function of GCN5 and/or USP22 in embryonic stem cells. Furthermore, it is unclear how tightly the functions of GCN5 and USP22 are linked or whether the HAT and/or Dub module functions in SCA7 disease. Addressing these questions will undoubtedly lead to a clearer understanding for the roles of SAGA in development and disease.

Acknowledgements

We thank Boyko Atanassov, Yi Chun Chen, Andria Schibler and Marenda Wilson-Pham for useful comments and discussions on the manuscript. Calley L. Hirsch is funded as an Odyssey Fellow supported by the Odyssey Program and the Houston Endowment Inc, Award for Scientific Achievement at The Univeristy of Texas M.D. Anderson Cancer Center. Parts of this work were supported by a grant from the NIH to SYRD, GM067718.

Glossary

Ada
adaptor
ATXN
ataxin
BrD
bromodomain
BTP
bromodomain transcription factors and PHD domain containing
CDK1
cyclin dependent kinase 1
Chk2
checkpoint kinase 2
ChrD
chromodomain
Ctk1
carboxy-terminal domain kinase 1
DEXDc
DEAD-like helicase
dE(y)2
Drosophila enhancer of yellow 2
Dub
deubiquitinase
EnY2
human ortholog of enhancer of yellow 2
FAT
FRAP/ATM/TRRAP domain
Gcn5
general control nonderepressible 5
HAT
histone acetyltransferase
Hdm2
human ortholog of mouse double minute 2 (mdm2)
LisH
Lis homology
nonstop
Drosophila analog of Ubp8
PCAF
p300/CBP-associated factor
PCNA
proliferating cell nuclear antigen
PI3Kc
phosphoinositide 3-kinase, catalytic domain
PIC
pre-initiation complex
Pol II
RNA polymerase II
PTM
post-translational modification
SAGA
Spt-Ada-Gcn5 acetyltranferase complex
SANT
Swi3/Ada2/N-CoR/TFIIIB domain
SCA7
spinocerebellar ataxia 7
Sgf
SAGA-associated factor
Spt
suppressor of Ty element
STAF
specificity protein transcription factor - associated factor
Sus1
S1 gene upstream of ySa1
SWIRM
Swi3/Rsc8/Moira domain
TAF
TATA binding protein-associated factor
TBP
TATA binding protein
TFIID
transcription factor II D
Tra1
yeast ortholog of human TRRAP
TREX/AMEX
transcription and export/mRNA and export
TRF1
telomeric-repeat binding factor 1
TRRAP
transformation/transcription domain-associated protein
Ubp8
ubiquitin specific processing protease
UBP-ZF
ubiquitin protease – zinc finger
UCH
ubiquitin carboxyl-terminal hydrolase
USP22
ubiquitin specific peptidase 22
WD40
40 amino acid protein domain often terminating in Trp-Asp
Wda
will decrease acetylation

Footnotes

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Contributor Information

Evangelia Koutelou, Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

Calley L. Hirsch, Program in Genes and Development, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

Sharon Y.R. Dent, Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

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35. Wang YL, Faiola F, Xu M, Pan S, Martinez E. Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. J Biol Chem. 2008;283:33808–33815. [PMC free article] [PubMed]
36. Guelman S, Kozuka K, Mao Y, Pham V, Solloway MJ, Wang J, Wu J, Lill JR, Zha J. The double-histone-acetyltransferase complex ATAC is essential for mammalian development. Mol Cell Biol. 2009;29:1176–1188. [PMC free article] [PubMed]
37. Qi D, Larsson J, Mannervik M. Drosophila Ada2b is required for viability and normal histone H3 acetylation. Mol Cell Biol. 2004;24:8080–8089. [PMC free article] [PubMed]
38•. Grau B, Popescu C, Torroja L, Ortuno-Sahagun D, Boros I, Ferrus A. Transcriptional adaptor ADA3 of Drosophila melanogaster is required for histone modification, position effect variegation, and transcription. Mol Cell Biol. 2008;28:376–385. [PMC free article] [PubMed]
[These authors highlight the importance of dAda3 in HAT function and Drosophila development. dAda3 is required for late larval stages and loss of dAda3 leads to decreased H3 and H4 acetylation levels at specific lysine residues. In addition, Ada3 is regulates the expression of specific genes.]
39. Guelman S, Suganuma T, Florens L, Weake V, Swanson SK, Washburn MP, Abmayr SM, Workman JL. The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation. Mol Cell Biol. 2006;26:7178–7189. [PMC free article] [PubMed]
40••. Mischerikow N, Spedale G, Altelaar AF, Timmers HT, Pijnappel WW, Heck AJ. In-depth profiling of post-translational modifications on the related transcription factor complexes TFIID and SAGA. J Proteome Res. 2009;8:5020–5030. [PubMed]
[This paper provides the first report of post-translational modifications specific to components of ySAGA. In particular, unique sites of protein acetylation and phosphorylation in ySAGA and TFIID were compared. Within the ySAGA complex, ySpt7 and ySgf73 were identified to be significantly acetylated, while ySpt7, yAda3, yTaf10, yTaf12 and ySgf73 were found to be phosphorylated.]
41. McMahon SB, Wood MA, Cole MD. The essential cofactor TRRAP recruits the histone acetyltransferase hGCN5 to c-Myc. Mol Cell Biol. 2000;20:556–562. [PMC free article] [PubMed]
42. Gamper AM, Roeder RG. Multivalent binding of p53 to the STAGA complex mediates coactivator recruitment after UV damage. Mol Cell Biol. 2008;28:2517–2527. [PMC free article] [PubMed]
43••. Liu X, Vorontchikhina M, Wang YL, Faiola F, Martinez E. STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation. Mol Cell Biol. 2008;28:108–121. [PMC free article] [PubMed]
[The authors show that hSAGA participates in Mediator recruitment during transcriptional activation. Myc functions to recruit the hSAGA components TRRAP and GCN5 to the TERT target gene for histone acetylation, in a manner independent of STAF65γ. However, STAF65γ is required for Myc recruitment of SPT3 and TAF9 to the TERT promoter. As well, both STAF65γ and Myc are needed to recruit the Mediator components MED1, MED16 and MED17 to the TERT gene. This data highlights the modularity of the hSAGA complex.]
44. Liu X, Tesfai J, Evrard YA, Dent SY, Martinez E. c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation. J Biol Chem. 2003;278:20405–20412. [PMC free article] [PubMed]
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48. Rosaleny LE, Ruiz-Garcia AB, Garcia-Martinez J, Perez-Ortin JE, Tordera V. The Sas3p and Gcn5p histone acetyltransferases are recruited to similar genes. Genome Biol. 2007;8:R119. [PMC free article] [PubMed]
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53. Johnsson A, Durand-Dubief M, Xue-Franzen Y, Ronnerblad M, Ekwall K, Wright A. HAT-HDAC interplay modulates global histone H3K14 acetylation in gene-coding regions during stress. EMBO Rep. 2009;10:1009–1014. [PMC free article] [PubMed]
54. Ingvarsdottir K, Krogan NJ, Emre NC, Wyce A, Thompson NJ, Emili A, Hughes TR, Greenblatt JF, Berger SL. H2B ubiquitin protease Ubp8 and Sgf11 constitute a discrete functional module within the Saccharomyces cerevisiae SAGA complex. Mol Cell Biol. 2005;25:1162–1172. [PMC free article] [PubMed]
55. Pascual-Garcia P, Govind CK, Queralt E, Cuenca-Bono B, Llopis A, Chavez S, Hinnebusch AG, Rodriguez-Navarro S. Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2. Genes Dev. 2008;22:2811–2822. [PMC free article] [PubMed]
56. Kurshakova MM, Krasnov AN, Kopytova DV, Shidlovskii YV, Nikolenko JV, Nabirochkina EN, Spehner D, Schultz P, Tora L, Georgieva SG. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. Embo J. 2007;26:4956–4965. [PMC free article] [PubMed]
57. Kohler A, Pascual-Garcia P, Llopis A, Zapater M, Posas F, Hurt E, Rodriguez-Navarro S. The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11. Mol Biol Cell. 2006;17:4228–4236. [PMC free article] [PubMed]
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[The authors found that USP22 and its Drosophila homolog nonstop use deubiquitinating activity on ubiquitinated H2A and H2B and this event facilitates the transactivation by the androgen receptor in human cells. They also identify the subunits of the mammalian Dub module and they observe that this deubiquitination activity enhances the positional effect variegation in Drosophila.]
59•. Zhang XY, Varthi M, Sykes SM, Phillips C, Warzecha C, Zhu W, Wyce A, Thorne AW, Berger SL, McMahon SB. The putative cancer stem cell marker USP22 is a subunit of the human SAGA complex required for activated transcription and cell-cycle progression. Molecular cell. 2008;29:102–111. [PMC free article] [PubMed]
[USP22 deubiquitinating activity is important for the transactivation of both Myc and p53 target genes, a function that explains USP22 identification as a member of the “11-gene-signature” of aggressive cancer types.]
60. Helmlinger D, Hardy S, Abou-Sleymane G, Eberlin A, Bowman AB, Gansmuller A, Picaud S, Zoghbi HY, Trottier Y, Tora L, et al. Glutamine-expanded ataxin-7 alters TFTC/STAGA recruitment and chromatin structure leading to photoreceptor dysfunction. PLoS Biol. 2006;4:e67. [PMC free article] [PubMed]
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66. Yamauchi T, Yamauchi J, Kuwata T, Tamura T, Yamashita T, Bae N, Westphal H, Ozato K, Nakatani Y. Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesis. Proc Natl Acad Sci U S A. 2000;97:11303–11306. [PMC free article] [PubMed]
67. Lin W, Srajer G, Evrard YA, Phan HM, Furuta Y, Dent SY. Developmental potential of Gcn5(-/-) embryonic stem cells in vivo and in vitro. Dev Dyn. 2007;236:1547–1557. [PubMed]
68. Tjeertes JV, Miller KM, Jackson SP. Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells. Embo J. 2009;28:1878–1889. [PMC free article] [PubMed]
69. Xie W, Song C, Young NL, Sperling AS, Xu F, Sridharan R, Conway AE, Garcia BA, Plath K, Clark AT, et al. Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells. Molecular cell. 2009;33:417–427. [PMC free article] [PubMed]
70•. Weake VM, Lee KK, Guelman S, Lin CH, Seidel C, Abmayr SM, Workman JL. SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system. Embo J. 2008;27:394–405. [PMC free article] [PubMed]
[In this study the Drosophila orthologs of the Dub module of SAGA nonstop and dSgf11 are analysed, providing strong evidence for the evolutionary conservation of SAGA complex among species and implicating the Drosophila SAGA complex in the regulation of accurate axon guidance in the optic lobe of the fly.]
71. Poeck B, Fischer S, Gunning D, Zipursky SL, Salecker I. Glial cells mediate target layer selection of retinal axons in the developing visual system of Drosophila. Neuron. 2001;29:99–113. [PubMed]
72. Kurabe N, Katagiri K, Komiya Y, Ito R, Sugiyama A, Kawasaki Y, Tashiro F. Deregulated expression of a novel component of TFTC/STAGA histone acetyltransferase complexes, rat SGF29, in hepatocellular carcinoma: possible implication for the oncogenic potential of c-Myc. Oncogene. 2007;26:5626–5634. [PubMed]
73. Patel JH, Du Y, Ard PG, Phillips C, Carella B, Chen CJ, Rakowski C, Chatterjee C, Lieberman PM, Lane WS, et al. The c-MYC oncoprotein is a substrate of the acetyltransferases hGCN5/PCAF and TIP60. Mol Cell Biol. 2004;24:10826–10834. [PMC free article] [PubMed]
74. Glinsky GV, Berezovska O, Glinskii AB. Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer. J Clin Invest. 2005;115:1503–1521. [PMC free article] [PubMed]
75. Shimada M, Niida H, Zineldeen DH, Tagami H, Tanaka M, Saito H, Nakanishi M. Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression. Cell. 2008;132:221–232. [PubMed]
76••. Atanassov BS, Evrard YA, Multani AS, Zhang Z, Tora L, Devys D, Chang S, Dent SY. Gcn5 and SAGA regulate shelterin protein turnover and telomere maintenance. Molecular cell. 2009;35:352–364. [PMC free article] [PubMed]
[The authors show that Gcn5 is implicated in telomeres maintenance by regulating components of the shelterin complex post-transcriptionally. They provide the first evidence for SAGA-dependent regulation of ubiquitination and protein turnover of TRF1, expanding the specificity and the targeting of SAGA complex Dub activity to non-histone substrates.]
77. Linares LK, Kiernan R, Triboulet R, Chable-Bessia C, Latreille D, Cuvier O, Lacroix M, Le Cam L, Coux O, Benkirane M. Intrinsic ubiquitination activity of PCAF controls the stability of the oncoprotein Hdm2. Nat Cell Biol. 2007;9:331–338. [PubMed]
78. Kass EM, Poyurovsky MV, Zhu Y, Prives C. Mdm2 and PCAF increase Chk2 ubiquitination and degradation independently of their intrinsic E3 ligase activities. Cell Cycle. 2009;8:430–437. [PubMed]
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