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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Neurosci. Author manuscript; available in PMC Jun 30, 2010.
Published in final edited form as:
Published online Feb 10, 2008. doi:  10.1038/nn2048
PMCID: PMC2894808
NIHMSID: NIHMS212330

Control of visually guided behavior by distinct populations of spinal projection neurons

Abstract

A basic question in the field of motor control is how different actions are represented by activity in spinal projection neurons. We used a new behavioral assay to identify visual stimuli that specifically drive basic motor patterns in zebrafish. These stimuli evoked consistent patterns of neural activity in the neurons projecting to the spinal cord, which we could map throughout the entire population using in vivo two-photon calcium imaging. We found that stimuli that drive distinct behaviors activated distinct subsets of projection neurons, consisting, in some cases, of just a few cells. This stands in contrast to the distributed activation seen for more complex behaviors. Furthermore, targeted cell by cell ablations of the neurons associated with evoked turns abolished the corresponding behavioral response. This description of the functional organization of the zebrafish motor system provides a framework for identifying the complete circuit underlying a vertebrate behavior.

In a behaving animal, the brain communicates its intentions to muscles via the pattern of activity in descending projection neurons1. In vertebrates, these cells respond to the detection and processing of sensory stimuli and transmit their motor command to the local networks of the spinal cord, which in turn initiate and coordinate muscle contraction2. A fundamental question in neuroscience is how the commands that initiate behaviors are encoded in the activation of these projection neurons35.

The spinal projection system of fish provides an excellent model for studying this code6. A diverse range of swimming behaviors can be seen in 6-d-old zebrafish79 that are mediated by a descending projection to the spinal cord consisting of fewer than 300 neurons10,11. These neurons are easily labeled with fluorescent indicators12, optically accessible with modern imaging techniques and arranged in a stereotyped pattern such that the same cells or groups of cells can easily be identified from one fish to the next13. These neurons are morphologically diverse, with distinct dendritic fields and axonal projection patterns13,14, suggesting that they serve different behavioral functions. Nevertheless, determining how differing patterns of activity in these spinal projection neurons produce different motor outputs has proved to be difficult.

An important step toward achieving this goal is to find ways to reliably and specifically activate distinct types of movement. One approach has been to use tactile stimuli to the head and tail of the zebrafish, which evoke different escape swims and differentially activate the segmental homologs of the Mauthner cell12. Activity in the Mauthner cell is sufficient to initiate escape sequences in goldfish15. Ablation of these neurons in zebrafish can eliminate short-latency, high-performance turns in response to tactile and acoustic stimuli, but the fish still show slower, long-latency responses16,17, and tactile stimuli have been found to activate virtually all reticulospinal neurons18. Similar widespread activation of motor-control neurons has been observed in both invertebrate19,20 and vertebrate model systems21,22. This suggests that, although small numbers of neurons may be sufficient to activate different behaviors23,24, the actual command is encoded in distributed activity throughout the population of control neurons2.

The swimming behaviors of zebrafish, including the complex sequence of turns and swims that make up the escape response, can be broken down into basic kinematic elements8. It is possible that the motor commands for these basic behaviors originate from distinct populations of neurons, which when combined would appear as a `distributed command'. We found that whole-field visual motion, with the direction dynamically locked to the fish's body axis, is able to selectively evoke some of these basic swim patterns. Calcium imaging of stimulus-evoked responses in the complete population of neurons projecting to the spinal cord revealed that these control neurons are indeed organized into distinct functional groups. We established a causal link between this observed organization and control of the fishes' behavior by targeted single-cell ablations.

RESULTS

Behavioral responses to stabilized whole-field motion

Larval zebrafish show a reliable optomotor response, turning and swimming to follow large moving gratings presented from below25. This requires that the fish respond to different stimulus directions with different swimming trajectories. To analyze the components of this complex behavior, we needed to eliminate the effect of the fishes' swimming on the stimulus orientation and motion. We achieved this by tracking the fishes' position and heading using a high-speed camera and translating and rotating the stimulus to cancel the fishes' own motion (Fig. 1a). Thus, if a fish viewing a right-to-left grating turned to the left, the stimulus rotated with him so that he continued to see leftward motion.

Figure 1
Behavioral responses to drifting gratings. (a) Schematic of the behavioral set up. (b) Average trajectories of six fish responding to gratings drifting at 24 equally spaced directions relative to their body axis. Trajectories are color coded according ...

We found that the fishes' swimming behavior was strongly tuned for stimulus direction (Fig. 1b). Stabilized gratings moving in 24 different directions relative to the fishes' body axis triggered 24 different swimming trajectories. Gratings moving from tail to head caused vigorous forward swimming; gratings that drift toward the fishes' left or right caused turning in the corresponding direction. The basic swim parameters, forward velocity and angular velocity, both showed strong tuning for stimulus direction (Fig. 1c,d).

Motor patterns underlying the optomotor response

Larval zebrafish swimming behavior occurs in discrete bouts alternating with brief periods of inactivity. These swimming bouts can be grouped into a small number of distinct categories, which have been characterized in detail8,9,26. We analyzed the visually evoked responses at the level of single swim bouts. We compared the high-speed kinematics of three typical swims evoked by different grating directions, a left turn, a right turn and a forward swim (Fig. 1e). The left and right turns consist of a sharp tail flick in one direction in which the tip of the tail bends around up to 180 degrees. This most closely resembles the previously described spontaneous routine turn8. The forward swim consists of ~30-Hz tail oscillations and corresponds to the `slow swim'8.

We compared the distribution of swimming bouts, characterized by two parameters, distance swum forward and angle turned, in response to moving gratings in eight different directions (Fig. 1f). In the center we have superimposed, for comparison, the distributions for forward (tail to head), back left and back right motion. We also plotted the same data using an additional parameter, left-to-right movement (Supplementary Fig. 1 online). Two features of the distributions were clear. First, the responses to each direction were highly clustered, indicating that they are stereotyped across trials and across fish. We observed that 84% of all swim bouts recorded during forward motion propelled the fish forward 0.5–4.0 mm with less than 9° of change in body angle. In contrast, 80% of bouts during back left motion were left turns larger than 45°. Second, there was less than 3% overlap between the distribution of swimming bouts evoked by forward motion and the turning responses shown superimposed in the center panel. From this, we conclude that whole-field drifting gratings can be used to isolate distinct components of zebrafish swimming behavior. Clustering of turns and forward swims was clearly seen when responses to all eight directions were combined (Supplementary Fig. 2 online). The stimulus tuning for each distinct behavior (Fig. 1g) was then compared with the stimulus tuning of neural responses. A complete mapping of neural activity required that the responses persist over multiple presentations of the stimuli. This was the case for the whole-field motion responses, which showed little or no reduction over time (Fig. 1h).

Calcium imaging of responses in spinal projection neurons

All motor commands from the fish's brain to its spinal cord must pass through the array of descending projection neurons. We used calcium imaging to monitor the activation of all such neurons during presentation of the behaviorally relevant stimuli described above. Two-photon excitation was essential, in part because the infrared excitation light was invisible to the fish, but also because it provides access to the deepest cells of the reticulospinal system. Responses were typically stable for several hours, allowing imaging at many focal planes and subsequent reconstruction of the response properties for the whole population.

We illustrate the analysis of such an experiment for a group of cells in the right rhombomere 1 (Fig. 2a). Two example cells are indicated and their calcium responses, averaged over their three-dimensional volume, are plotted (Fig. 2b). The stimulus was a sinusoidal grating that drifted at 1 Hz for 10 s in one of eight directions (shaded regions) and was stationary for 17 s in between. The responses of these two cells were clearly selective for different directions. Notably, all the projection neurons with stimulus-evoked responses (>0.1 Δf/f to at least one direction) had strong direction selectivity (Supplementary Fig. 3 online). We plotted the cells' responses, in polar form, as the average percentage increase in fluorescence for each stimulus direction and also as a `directionality vector'27 (Fig. 2c). We summarized the results of this analysis in a color-coded image (Fig. 2d).

Figure 2
Measuring calcium responses in spinal projection neurons. (a) Z projection of data acquired during calcium imaging at different z planes. The image is shown with inverted luminance here and in subsequent figures for visual clarity; all images are presented ...

The end result of such an analysis for one fish with scattered labeling throughout the spinal projection system is shown in Figure 2e. Most cells either did not respond or preferred forward motion. A few cells preferred gratings moving in directions that elicited turning. The organization of forward motion–responsive cells seen in this fish was repeated in all of the individuals that we examined. We divided the cells into 20 morphological groups for analysis (a schematic of our classification system is shown in Fig. 2f).

Projection neuron responses to whole-field motion

Identifying the neural correlates of the zebrafish whole-field motion response required a complete mapping of the activity of projection neurons in response to drifting gratings. We analyzed responses from 1,465 neurons in 50 fish (Fig. 3). The cells fell into distinct functional groups. The stimulus tuning of each group could be compared with the stimulus tuning of the behavioral patterns (Fig. 1g), allowing us to hypothesize which of these neurons might participate in each behavior.

Figure 3
Summary of direction tuning of all spinal projection neurons. Top left, schematic summary of the directional preference of spinal projection neurons. Cells that preferred tail-to-head motion are colored green, cells that had a left or right preference ...

Cells involved in initiating slow swims should be selectively activated by forward motion and show bilaterally symmetric responses. Both of these properties were seen for cells in the nucleus of the medial longitudinal fasciculus (nucMLF), including the large MeL and MeM cells, RoL1, RoR1 and RoM1c. In every fish, forward-preferring neurons were scattered throughout the nucMLF (three examples of well-labeled nucMLF cells are shown in Fig. 4a). Cells of the MiR and MiM types also occasionally showed weak forward responses, but this was not consistent across fish.

Figure 4
Spatial distribution of visually responsive cells. Examples of the response distribution in different fish are shown here. (a) Bilaterally labeled nucMLF cells in three different fish with a z projection on the left and color coding by directionality ...

We expected neurons involved in turning to show two characteristics. They should be activated by stimuli that evoke turns (Fig. 1g), and their responses should be lateralized; a left-preferring neuron should have a contralateral partner with the opposite preference. In only two places were these conditions met: in RoM1r and in the ventromedial cells of rhombomeres 3–5 (red/green/blue box). Cells in the RoM1r group were small and not always well labeled. However, they were clearly identifiable by shape and location and showed notable lateralized response properties selective for `turn' stimuli. The spatial organization of this direction preference in rhombomere 1 was consistent from fish to fish (Fig. 4b).

The average tuning curve of the ventromedial cells included both bilateral forward-motion responses and lateralized responses to left and right stimuli. However, the response vectors of these cells spanned a wide range of directions. This implies that these cells, although grouped together on the basis of anatomical features, are, in fact, functionally heterogeneous. This was best demonstrated by looking at all ventromedial cells individually in a single fish (Fig. 4c). These clusters contained mixtures of neurons with different response properties. Many cells showed a strong forward-motion preference, but some showed a lateralized preference for turning stimuli. Therefore, this group could be involved in generating both slow swims and turns. We plotted the distribution of preferred directions for neurons in responsive groups (Supplementary Fig. 3), illustrating the bimodal distribution of preferred directions in the ventromedial group. The remaining groups of cells showed no consistent responses, although most of these have been shown to have calcium responses to tactile stimuli18 and are likely to participate in other behaviors.

Laser ablation of turning neurons

Calcium imaging revealed that projection neurons were organized into distinct functional groups related to distinct behaviors, which suggests that removing one of these groups should result in a loss of its associated behavioral response. To test for the expected causal relationship, we chose to remove neurons responsive to `turn' stimuli, RoM1r and ventromedial cells, as there are relatively few and the two laterally symmetrical populations provide an internal control. To remove the selected cells, we adapted a technique28 based on nonlinear absorption for the rapid ablation of single identified neurons in the zebrafish. A mode-locked laser is scanned in a spiral pattern over a selected cell until a highly localized plasma is formed29, destroying the target but leaving adjacent cells intact (Fig. 5a–c). To test the efficacy of this technique, we ablated the Mauthner cell and its segmental homologs, reproducing the side-specific increase in escape response latency that was found in previous studies16 (Supplementary Movie 1 online).

Figure 5
Laser ablation of turning-selective neurons. (a) Z projection of spinal projection neurons labeled with Texas Red dextran. (b,c) A right RoM2l neuron before and after laser ablation. The cell and its processes were destroyed and cell debris is visible. ...

We measured the behavioral responses of fish, before and after ablation, to stimuli that reliably evoked turning, backward left and backward right motion. To test the behavior around the threshold for responding, we ramped up the contrast of the stimuli from 0 to 20% over 20 s. As an additional control, we unilaterally ablated all of the large RoM cells in rhombomeres 2–3, as they showed no grating responses. Fish were always able to turn in both directions after this control ablation (Fig. 5d–f). For the ventromedial cells, we unilaterally ablated 2–4 cells from each cluster, as we couldn't predict by morphology which would show the turning responses or guarantee that all cells were labeled. Nevertheless, in several cases, this resulted in a complete elimination of turning toward the ablated side (Fig. 5g–l). In cases where turning toward the ablated side was eliminated, forward swims and turns away from the ablated side were still performed normally (Supplementary Fig. 4 online). With this stochastic method, we saw, on average, a large, significant decrease in turning whenever we ablated cells in these clusters (P<0.001; Fig. 5m). We observed a >90% reduction in turning toward the ablated side in 7 out of 23 ablations. Three fish had a <10% reduction and the rest had intermediate, graded phenotypes. No significant change in turning was observed when only the RoM1r cells were ablated (P > 0.05). We therefore argue that this small subpopulation of functionally lateralized ventromedial neurons is a necessary component of the circuit underlying turning in the optomotor response.

DISCUSSION

This study identifies groups of spinal projection neurons that are selective for visual stimuli that evoke basic swim patterns in zebrafish, and reveals, with cellular detail, the spatial organization of a vertebrate motor-control system. These functional groups are distinct and composed of an unexpectedly small number of neurons. The discrete organization that we see, which differs from previously observed distributed activity4,18,30, is made apparent by the ability to evoke simple motor patterns with controlled visual stimulation. The fact that more complex behaviors are associated with distributed activation of many neurons is consistent with a model in which subsets of neurons initiate distinct components of the behavior31,32.

Additional evidence for a distributed motor command has come from a number of studies that found that ablations do not abolish specific behaviors, although they can alter their latency and kinematics16,17,3335. Here, an ablation guided by an observed functional organization was able to completely abolish a specific behavior; optomotor turns in one direction were specifically eliminated by ablation of the small number of cells responsive to turn-evoking stimuli. Why do we see such a strong phenotype? Two aspects of the approach contributed to this: dynamically stabilized visual stimuli were used to elicit very specific motor patterns and responses in all of the projection neurons were assessed, thereby identifying all possible participants in the behavior.

This characterization of a functionally organized motor-control system presents new opportunities to address the development and function of systems underlying vertebrate behavior. Zebrafish are a well-established model system for vertebrate development. Hundreds of thousands of neurons and their synaptic connections are formed in the first few days of the zebrafish's life. A previous study36 grouped reticulospinal neurons according to the timing of their axonal growth. Notably, there is a one to one correspondence between the classification of that study and ours; all neurons in the developmental categories `nucMLF' and `second wave' showed responses to forward motion, whereas all others did not. Therefore, the projection neurons active during forward slow swims constitute a distinct developmental, as well as functional, group. Interestingly, the spinal interneurons involved in slow swimming behavior also appear a few hours later than those involved in escapes37.

The spinal projection neurons link sensory processing in the brain to motor output in the spinal cord and, therefore, provide an excellent starting point for studying the sensorimotor transformations underlying behavior. Having identified which neurons control particular motor patterns, we can now ask how their activity is decoded in the spinal cord to produce the associated behavior. Substantial progress has been made recently in understanding the organization and function of specific cell types and circuits in the zebrafish spinal cord3840, encouraging the hope that a connection between descending motor commands and the resulting motor patterns is within reach. More obscure, however, is the upstream circuitry that leads to the selective activation of these descending control neurons. Recently developed optical techniques, which are capable of revealing activity and connectivity in large numbers of neurons4143, can now be focused on determining the inputs to neurons with known response selectivities and identified roles in behavior. Together, this opens up the possibility of studying complete circuits underlying complex behaviors in a vertebrate with a small, transparent brain.

METHODS

Zebrafish

We used 6–7-d-old zebrafish from AB and WIK strains in all experiments. mitfa−/− (nacre) fish44 were used for imaging and ablation studies, as they lack pigment in the skin, but retain normal eye pigmentation. Their behavior is indistinguishable from wild-type siblings in our assay. All experiments were approved by Harvard University's Standing Committee on the Use of Animals in Research and Training.

Behavioral setup

Zebrafish larvae swam freely in a 5-cm diameter acrylic arena. Fish were illuminated by an array of infrared light-emitting diodes and their swimming behavior was recorded at 200 Hz using an infrared-sensitive, high-speed CMOS camera (Mikrotron). Stimuli were projected directly onto a 10-cm screen 5 mm below the fish using a DLP projector (Optoma). Custom image-processing software (Visual C++, Microsoft) extracted the position and orientation of the fish at the acquisition frame rate. This information was used to update a stimulus rendered in real-time using DirectX3D (Microsoft).

Calcium imaging

Spinal projection neurons were filled as previously described12. Briefly, a 50% (w/v) solution of dextran-conjugated calcium green (Invitrogen) was injected into the spinal cord of tricaine-anesthetized fish 24 h before imaging. Fish were embedded in 1.5% low melting-temperature agarose, paralyzed using an injection of α-bungarotoxin and imaged with a custom two-photon microscope45, which used a pulsed Ti-sapphire laser tuned to 920 nm (Spectra Physics). The stimulus was projected from below with a DLP projector (Optoma) and passed through a red long-pass filter, which allowed simultaneous visual stimulation and fluorescence detection. In each experiment, frames were acquired at 3.6 Hz. After one or more repetitions of each stimulus, the focus was moved to a different z plane. The resulting time series were combined to yield a four-dimensional picture of the scanned volume of the brain. This image was passed through image segmentation filters in Matlab (Mathworks) to extract the volume occupied by each cell. Cell identities were assigned by hand on the basis of morphological criteria13. Cells with an evoked response greater than 0.1 Δf/f to at least one stimulus direction were assigned directionality vectors and a directional preference index (DPI) calculated as in a previous study27.

Two-photon laser ablation

Targeted cells were identified and a central subregion was selected from a full frame scan. The power of a mode-locked laser (850 nm) was linearly increased while the beam was scanned in a spiral pattern throughout the targeted region. Scanning was immediately terminated on the detection of brief flashes of saturating intensity, which are presumed to result from the creation of a highly localized plasma via multi-photon absorption by water molecules28,29. This procedure always results in destruction of the cell despite immediately adjacent cells appearing unaffected, as verified using both a pan-neuronally expressing transgenic line (HuC:YC2.1)43 and retrogradely labeled spinal projection cells.

Supplementary Material

supp

ACKNOWLEDGMENTS

The authors would like to thank M. Meister, T. Bonhoeffer, J.R. Sanes, B.P. Olveczky, M.C. Smear and J.E. Dowling for valuable comments on the manuscript, D.M. O'Malley for advice on experimental techniques, A.F. Schier for generous help with fish rearing and O.C. Orger for assistance with data analysis. This work was supported by postdoctoral fellowships from the Helen Hay Whitney Foundation (M.B.O.) and Human Frontier Science Program (J.H.B.), and US National Institutes of Health grant R01 EY014429-01A2 and funding from the McKnight and Dana Foundations (F.E.).

Footnotes

Note: Supplementary information is available on the Nature Neuroscience website.

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