• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Apr 1980; 34(1): 104–107.
PMCID: PMC288675

Roles of bacteriophage T4 gene 5 and gene s products in cell lysis.

Abstract

Previous studies indicated that (i) T4 gene s product (gps) protects infected cells from superinfection lysis from without, (ii) the absence of gps in infected cells also leads to lysis from within even when T4 e lysozyme is absent, (iii) T4 gene 5 product (gp5), a polypeptide of the virion baseplate, may be responsible for inducing lysis from without, and (iv) altered gp5 of the T4 mutant 5ts1 can replace e lysozyme to cause lysis from within. Results of this study showed that (i) wild-type gp5 in infected cells lacking e lysozyme was responsible for lysis from within in the absence of gps, and (ii) gps did not protect infected cells from superinfection lysis from without by 5ts1 phage. We prpose that gps normally prevents functional expression of wild-type gp5 activity from either side of the cell wall, whereas the 5ts1 form of gp5 is insensitive to the gps barrier and induces lysis from either side of the cell wall.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (610K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bayer ME. Adsorption of bacteriophages to adhesions between wall and membrane of Escherichia coli. J Virol. 1968 Apr;2(4):346–356. [PMC free article] [PubMed]
  • Bode W. Lysis inhibition in Escherichia coli infected with bacteriophage T4. J Virol. 1967 Oct;1(5):948–955. [PMC free article] [PubMed]
  • Doermann AH. Lysis and Lysis Inhibition with Escherichia coli Bacteriophage. J Bacteriol. 1948 Feb;55(2):257–276. [PMC free article] [PubMed]
  • EDGAR RS, FEYNMAN RP, KLEIN S, LIELAUSIS I, STEINBERG CM. Mapping experiments with r mutants of bacteriophage T4D. Genetics. 1962 Feb;47:179–186. [PMC free article] [PubMed]
  • Emrich J. Lysis of T4-infected bacteria in the absence of lysozyme. Virology. 1968 May;35(1):158–165. [PubMed]
  • Emrich J, Streisinger G. The role of phage lysozyme in the life cycle of phage T4. Virology. 1968 Nov;36(3):387–391. [PubMed]
  • Kao SH, McClain WH. Baseplate protein of bacteriophage T4 with both structural and lytic functions. J Virol. 1980 Apr;34(1):95–103. [PMC free article] [PubMed]
  • Kikuchi Y, King J. Genetic control of bacteriophage T4 baseplate morphogenesis. III. Formation of the central plug and overall assembly pathway. J Mol Biol. 1975 Dec 25;99(4):695–716. [PubMed]
  • Mukai F, Streisinger G, Miller B. The mechanism of lysis in phage T4-infected cells. Virology. 1967 Nov;33(3):398–404. [PubMed]
  • Simon LD, Anderson TF. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. I. Attachment and penetration. Virology. 1967 Jun;32(2):279–297. [PubMed]
  • VISCONTI N. Resistance to lysis from without in bacteria infected with T2 bacteriophage. J Bacteriol. 1953 Sep;66(3):247–253. [PMC free article] [PubMed]
  • Yamazaki Y. Enzymatic activities on cell walls in bacteriophage T4. Biochim Biophys Acta. 1969 May 27;178(3):542–550. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles