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Proc Natl Acad Sci U S A. Jun 1989; 86(12): 4397–4401.
PMCID: PMC287276

tRNA-tRNA interactions within cellular ribosomes.

Abstract

We describe an assay that converts the effects of tRNA-tRNA contacts at two particular codons into a quantitative effect on beta-galactosidase level. The assay measures the separate and combined efficiency of suppression at adjacent nonsense codons in vivo using a set of specially created homologous messages. In a survey of distal anticodon arm substitutions, we find that particular mutant tRNAs occupying the P-site reduce the apparent efficiency of the suppressor tRNA reading the A-site codon by factors of 2-170. By using measured tRNA-tRNA distances and the crystallographic tRNA structure, we propose a model of the tRNA-tRNA-mRNA complex. In the model, the anticodon loops of the P-site and A-site tRNAs contact one another in a way that is consistent with our combined tRNA efficiency data. These results suggest that tRNA-tRNA interactions that modulate tRNA action are an inevitable feature of translation.

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Selected References

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  • Fairclough RH, Cantor CR. The distance between the anticodon loops of two tRNAs bound to the 70 S Escherichia coli ribosome. J Mol Biol. 1979 Aug 25;132(4):575–586. [PubMed]
  • Johnson AE, Adkins HJ, Matthews EA, Cantor CR. Distance moved by transfer RNA during translocation from the A site to the P site on the ribosome. J Mol Biol. 1982 Mar 25;156(1):113–140. [PubMed]
  • Paulsen H, Robertson JM, Wintermeyer W. Topological arrangement of two transfer RNAs on the ribosome. Fluorescence energy transfer measurements between A and P site-bound tRNAphe. J Mol Biol. 1983 Jun 25;167(2):411–426. [PubMed]
  • Bossi L. Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message. J Mol Biol. 1983 Feb 15;164(1):73–87. [PubMed]
  • Miller JH, Albertini AM. Effects of surrounding sequence on the suppression of nonsense codons. J Mol Biol. 1983 Feb 15;164(1):59–71. [PubMed]
  • Murgola EJ, Pagel FT, Hijazi KA. Codon context effects in missense suppression. J Mol Biol. 1984 May 5;175(1):19–27. [PubMed]
  • Yarus M, Folley LS. Sense codons are found in specific contexts. J Mol Biol. 1985 Apr 20;182(4):529–540. [PubMed]
  • Smith D, Yarus M. Transfer RNA structure and coding specificity. I. Evidence that a D-arm mutation reduces tRNA dissociation from the ribosome. J Mol Biol. 1989 Apr 5;206(3):489–501. [PubMed]
  • Yarus M, Cline SW, Wier P, Breeden L, Thompson RC. Actions of the anticodon arm in translation on the phenotypes of RNA mutants. J Mol Biol. 1986 Nov 20;192(2):235–255. [PubMed]
  • Yarus M. Translational efficiency of transfer RNA's: uses of an extended anticodon. Science. 1982 Nov 12;218(4573):646–652. [PubMed]
  • Holbrook SR, Sussman JL, Warrant RW, Kim SH. Crystal structure of yeast phenylalanine transfer RNA. II. Structural features and functional implications. J Mol Biol. 1978 Aug 25;123(4):631–660. [PubMed]
  • Vacher J, Springer M, Buckingham RH. Functional mutants of phenylalanine transfer RNA from Escherichia coli. EMBO J. 1985 Feb;4(2):509–513. [PMC free article] [PubMed]
  • Woese C. Molecular mechanics of translation: a reciprocating ratchet mechanism. Nature. 1970 May 30;226(5248):817–820. [PubMed]
  • McDonald JJ, Rein R. A stereochemical model of the transpeptidation complex. J Biomol Struct Dyn. 1987 Apr;4(5):729–744. [PubMed]
  • Dock-Bregeon AC, Chevrier B, Podjarny A, Moras D, deBear JS, Gough GR, Gilham PT, Johnson JE. High resolution structure of the RNA duplex [U(U-A)6A]2. Nature. 1988 Sep 22;335(6188):375–378. [PubMed]
  • Lacatena RM, Cesareni G. Interaction between RNA1 and the primer precursor in the regulation of Co1E1 replication. J Mol Biol. 1983 Nov 5;170(3):635–650. [PubMed]
  • Normanly J, Masson JM, Kleina LG, Abelson J, Miller JH. Construction of two Escherichia coli amber suppressor genes: tRNAPheCUA and tRNACysCUA. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6548–6552. [PMC free article] [PubMed]

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