• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Apr 1989; 86(7): 2190–2194.
PMCID: PMC286877

Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation.


A three-dimensional crystal structure of the biotin-binding core of streptavidin has been determined at 3.1-A resolution. The structure was analyzed from diffraction data measured at three wavelengths from a single crystal of the selenobiotinyl complex with streptavidin. Streptavidin is a tetramer with subunits arrayed in D2 symmetry. Each protomer is an 8-stranded beta-barrel with simple up-down topology. Biotin molecules are bound at one end of each barrel. This study demonstrates the effectiveness of multiwavelength anomalous diffraction (MAD) procedures for macromolecular crystallography and provides a basis for detailed study of biotin-avidin interactions.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Green NM. Avidin. Adv Protein Chem. 1975;29:85–133. [PubMed]
  • Pähler A, Hendrickson WA, Kolks MA, Argaraña CE, Cantor CR. Characterization and crystallization of core streptavidin. J Biol Chem. 1987 Oct 15;262(29):13933–13937. [PubMed]
  • Gope ML, Keinänen RA, Kristo PA, Conneely OM, Beattie WG, Zarucki-Schulz T, O'Malley BW, Kulomaa MS. Molecular cloning of the chicken avidin cDNA. Nucleic Acids Res. 1987 Apr 24;15(8):3595–3606. [PMC free article] [PubMed]
  • Bayer EA, Wilchek M. The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem Anal. 1980;26:1–45. [PubMed]
  • Weber PC, Cox MJ, Salemme FR, Ohlendorf DH. Crystallographic data for Streptomyces avidinii streptavidin. J Biol Chem. 1987 Sep 15;262(26):12728–12729. [PubMed]
  • DeTitta GT, Parthasarathy R, Blessing RH, Stallings W. Carboxybiotin translocation mechanisms suggested by diffraction studies of biotin and its vitamers. Proc Natl Acad Sci U S A. 1980 Jan;77(1):333–337. [PMC free article] [PubMed]
  • Hofmann K, Titus G, Montibeller JA, Finn FM. Avidin binding of carboxyl-substituted biotin and analogues. Biochemistry. 1982 Mar 2;21(5):978–984. [PubMed]
  • Hendrickson WA, Smith JL, Phizackerley RP, Merritt EA. Crystallographic structure analysis of lamprey hemoglobin from anomalous dispersion of synchrotron radiation. Proteins. 1988;4(2):77–88. [PubMed]
  • Kahn R, Fourme R, Bosshard R, Chiadmi M, Risler JL, Dideberg O, Wery JP. Crystal structure study of Opsanus tau parvalbumin by multiwavelength anomalous diffraction. FEBS Lett. 1985 Jan 1;179(1):133–137. [PubMed]
  • Korszun ZR. The tertiary structure of azurin from Pseudomonas denitrificans as determined by Cu resonant diffraction using synchrotron radiation. J Mol Biol. 1987 Jul 20;196(2):413–419. [PubMed]
  • Guss JM, Merritt EA, Phizackerley RP, Hedman B, Murata M, Hodgson KO, Freeman HC. Phase determination by multiple-wavelength x-ray diffraction: crystal structure of a basic "blue" copper protein from cucumbers. Science. 1988 Aug 12;241(4867):806–811. [PubMed]
  • Murthy HM, Hendrickson WA, Orme-Johnson WH, Merritt EA, Phizackerley RP. Crystal structure of Clostridium acidi-urici ferredoxin at 5-A resolution based on measurements of anomalous X-ray scattering at multiple wavelengths. J Biol Chem. 1988 Dec 5;263(34):18430–18436. [PubMed]
  • Templeton LK, Templeton DH. Biaxial tensors for anomalous scattering of X-rays in selenolanthionine. Acta Crystallogr A. 1988 Nov 1;44(Pt 6):1045–1051. [PubMed]
  • Hendrickson WA, Smith JL, Sheriff S. Direct phase determination based on anomalous scattering. Methods Enzymol. 1985;115:41–55. [PubMed]
  • Newcomer ME, Jones TA, Aqvist J, Sundelin J, Eriksson U, Rask L, Peterson PA. The three-dimensional structure of retinol-binding protein. EMBO J. 1984 Jul;3(7):1451–1454. [PMC free article] [PubMed]
  • Jones TA, Bergfors T, Sedzik J, Unge T. The three-dimensional structure of P2 myelin protein. EMBO J. 1988 Jun;7(6):1597–1604. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...