• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Feb 1989; 86(4): 1254–1258.
PMCID: PMC286666

Fourier analysis of cell motility: correlation of motility with metastatic potential.

Abstract

We report the development of a computerized, mathematical system for quantitating the various types of cell motility. This Fourier analysis method simultaneously quantifies for individual cells (i) temporal changes in cell shape represented by cell ruffling, undulation, and pseudopodal extension, (ii) cell translation, and (iii) average cell size and shape. This spatial-temporal Fourier analysis was tested on a series of well-characterized animal tumor cell lines of rat prostatic cancer to study in a quantitative manner the correlation of cell motility with increasing in vivo metastatic potential. Fourier motility coefficients measuring pseudopodal extension correlated best with metastatic potential in the cell lines studied. This study demonstrated that Fourier analysis provides quantitative measurement of cell motility that may be applied to the study of biological processes. This analysis should aid in the study of the motility of individual cells in various areas of cellular and tumor biology.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Porter KR, Todaro GJ, Fonte V. A scanning electron microscope study of surface features of viral and spontaneous transformants of mouse Balb-3T3 cells. J Cell Biol. 1973 Dec;59(3):633–642. [PMC free article] [PubMed]
  • Ambros VR, Chen LB, Buchanan JM. Surface ruffles as markers for studies of cell transformation by Rous sarcoma virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3144–3148. [PMC free article] [PubMed]
  • Bar-Sagi D, Feramisco JR. Induction of membrane ruffling and fluid-phase pinocytosis in quiescent fibroblasts by ras proteins. Science. 1986 Sep 5;233(4768):1061–1068. [PubMed]
  • Myrdal SE, Auersperg N. p21ras. Heterogeneous localization in transformed cells. Exp Cell Res. 1985 Aug;159(2):441–450. [PubMed]
  • Goshima K, Masuda A, Owaribe K. Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport. J Cell Biol. 1984 Mar;98(3):801–809. [PMC free article] [PubMed]
  • Heine UI, Keski-Oja J, Wetzel B. Rapid membrane changes in mouse epithelial cells after exposure to epidermal growth factor. J Ultrastruct Res. 1981 Dec;77(3):335–343. [PubMed]
  • Myrdal SE, Twardzik DR, Auersperg N. Cell-mediated co-action of transforming growth factors: incubation of type beta with normal rat kidney cells produces a soluble activity that prolongs the ruffling response to type alpha. J Cell Biol. 1986 Apr;102(4):1230–1234. [PMC free article] [PubMed]
  • BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. [PMC free article] [PubMed]
  • Albrecht-Buehler G. The phagokinetic tracks of 3T3 cells. Cell. 1977 Jun;11(2):395–404. [PubMed]
  • Nelson RD, Quie PG, Simmons RL. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed]
  • Gail MH, Boone CW. The locomotion of mouse fibroblasts in tissue culture. Biophys J. 1970 Oct;10(10):980–993. [PMC free article] [PubMed]
  • Dunn GA, Brown AF. Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci. 1986 Jul;83:313–340. [PubMed]
  • Dunn GA, Brown AF. A unified approach to analysing cell motility. J Cell Sci Suppl. 1987;8:81–102. [PubMed]
  • Mohler JL, Partin AW, Isaacs WB, Coffey DS. Time lapse videomicroscopic identification of Dunning R-3327 adenocarcinoma and normal rat prostate cells. J Urol. 1987 Mar;137(3):544–547. [PubMed]
  • Isaacs JT, Isaacs WB, Feitz WF, Scheres J. Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate. 1986;9(3):261–281. [PubMed]
  • Mareel MM. Recent aspects of tumor invasiveness. Int Rev Exp Pathol. 1980;22:65–129. [PubMed]
  • Sträuli P, Haemmerli G. The role of cancer cell motility in invasion. Cancer Metastasis Rev. 1984;3(2):127–141. [PubMed]
  • GEY GO. Some aspects of the constitution and behavior of normal and malignant cells maintained in continuous culture. Harvey Lect. 1954;50:154–229. [PubMed]
  • Guirguis R, Margulies I, Taraboletti G, Schiffmann E, Liotta L. Cytokine-induced pseudopodial protrusion is coupled to tumour cell migration. Nature. 1987 Sep 17;329(6136):261–263. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • OMIM
    OMIM
    OMIM record citing PubMed
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...