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Proc Natl Acad Sci U S A. Sep 1969; 64(1): 155–160.
PMCID: PMC286140

DIABETES MELLITUS AND AGING: DIMINISHED PLATING EFFICIENCY OF CULTURED HUMAN FIBROBLASTS*

Abstract

This work concerns the effect of age and the diabetic gene(s) on the growth capacity of skin fibroblasts in culture. Cells from normal subjects and the progeny of conjugal diabetics have similar lifespans after multiple passages in mass culture. The combined lifespans in vitro are inversely proportional to the age of the donor. When individual cells are plated, more of those from normal subjects are able to form colonies. The difference in plating efficiency is apparent when first tested after 20 generations of growth, persists at 30 and 40 generations, but disappears after 50 generations. This cell culture system should be useful in studying the inheritance of the diabetic gene(s), the pathogenesis of the diabetic state, and the relationship between aging and diabetes, both of which decrease plating efficiency.

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Selected References

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  • Rimoin DL. Genetics of diabetes mellitus. Diabetes. 1967 May;16(5):346–351. [PubMed]
  • SIMPSON NE. MULTIFACTORIAL INHERITANCE: A POSSIBLE HYPOTHESIS FOR DIABETES. Diabetes. 1964 Sep-Oct;13:462–471. [PubMed]
  • HAYNER NS, KJELSBERG MO, EPSTEIN FH, FRANCIS T., Jr CARBOHYDRATE TOLERANCE AND DIABETES IN A TOTAL COMMUNITY, TECUMSEH, MICHIGAN. 1. EFFECTS OF AGE, SEX, AND TEST CONDITIONS ON ONE-HOUR GLUCOSE TOLERANCE IN ADULTS. Diabetes. 1965 Jul;14:413–423. [PubMed]
  • Gottlieb MS, Root HF. Diabetes mellitus in twins. Diabetes. 1968 Nov;17(11):693–704. [PubMed]
  • SWIM HE, PARKER RF. Culture characteristics of human fibroblasts propagated serially. Am J Hyg. 1957 Sep;66(2):235–243. [PubMed]
  • HAYFLICK L. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. Exp Cell Res. 1965 Mar;37:614–636. [PubMed]
  • Goldstein S. Lifespan of cultured cells in progeria. Lancet. 1969 Feb 22;1(7591):424–424. [PubMed]
  • Goldstein S, Littlefield JW. Effect of insulin on the conversion of glucose-C-14 to C-14-O2 by normal and diabetic fibroblasts in culture. Diabetes. 1969 Aug;18(8):545–549. [PubMed]
  • Fratantoni JC, Hall CW, Neufeld EF. Hurler and Hunter syndromes: mutual correction of the defect in cultured fibroblasts. Science. 1968 Nov 1;162(3853):570–572. [PubMed]
  • Sheft I, Spittler TM, Martin FH. Xenon Hexafluoride: Preparation of Pure Form and Melting Point. Science. 1964 Aug 14;145(3633):701–702. [PubMed]
  • Waymouth C. Somatic cells in vitro: their relationship to progenitive cells and to artificial milieux. Natl Cancer Inst Monogr. 1967 Sep;26:1–21. [PubMed]
  • Papayannopoulou TG, Martin GM. Alkaline phosphatase "constitutive" clones: evidence for de-novo heterogeneity of established human skin fibroblast strains. Exp Cell Res. 1967 Jan;45(1):72–84. [PubMed]
  • PIOUS DA, HAMBURGER RN, MILLS SE. CLONAL GROWTH OF PRIMARY HUMAN CELL CULTURES. Exp Cell Res. 1964 Feb;33:495–507. [PubMed]
  • Macieira-Coelho A, Pontén J, Philipson L. The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro. Exp Cell Res. 1966 Jun;42(3):673–684. [PubMed]

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