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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Neurosci. Author manuscript; available in PMC Oct 7, 2010.
Published in final edited form as:
PMCID: PMC2856701
NIHMSID: NIHMS193322

In vivo Deletion of Immunoglobulin Domains 5-6 in Neurofascin (Nfasc) Reveals Domain Specific Requirements in Myelinated Axons

Abstract

The formation of paranodal axo-glial junctions is critical for the rapid and efficient propagation of nerve impulses. Genetic ablation of genes encoding the critical paranodal proteins Caspr, contactin (Cont), and the myelinating glia-specific isoform of Neurofascin (NfascNF155) results in the disruption of the paranodal axo-glial junctions, loss of ion channel segregation, and impaired nerve conduction, but the mechanisms regulating their interactions remains elusive. Here, we report that loss of immunoglobulin (Ig) domains 5 and 6 in NfascNF155 in mice phenocopies complete ablation of NfascNF155. The mutant mice lack paranodal septate junctions, resulting in the diffusion of Caspr and Cont from the paranodes, and redistribution of the juxtaparanodal potassium channels towards the nodes. While critical for NfascNF155 function, we find that Ig5-6 are dispensable for nodal NfascNF186 function. Moreover, in vitro binding assays using Ig5-6 deletion constructs reveal their importance for the association of NfascNF155 with Cont. These findings provide the first molecular evidence demonstrating domain specific requirements controlling the association of the paranodal tripartite complex in vivo. Our studies further emphasize that in vivo structure/function analysis is necessary to define the unique protein/protein interactions that differentially regulate the functions of Neurofascins during axonal domain organization.

Keywords: Myelination, neuron-glia, septate junctions, Caspr, Contactin, Neurofascin

Introduction

The organization of ion channels within specific subcellular domains along myelinated axons is essential for proper nerve conduction. Specialized axo-glial septate-like junctions, formed between the glial paranodal loops and the underlying axolemma, mediate the segregation of ion channels within myelinated axons (Salzer, 2003; Thaxton and Bhat, 2009). While numerous proteins reside within the paranodes, to date, only three proteins have been identified as integral components of the paranodal septate junctions: Cont and Caspr in the axon, and the 155kDa isoform of Neurofascin (NfascNF155) expressed specifically in myelinating glia (Menegoz et al., 1997; Bhat et al., 2001; Boyle et al., 2001; Sherman et al., 2005; Zonta et al., 2008; Pillai et al., 2009). Genetic ablation of the genes encoding these proteins in mice results in loss of axo-glial junctions, ion channel disorganization, impaired nerve conduction, and ultimately axonal degeneration (Bhat et al., 2001; Boyle et al., 2001; Pillai et al., 2009). Although characterized for their requirement in paranodal organization, the mechanisms regulating the potential interactions between Cont, Caspr, and NfascNF155 remain unclear.

Myelinating glia-specific ablation of NfascNF155 in a spatio-temporal manner resulted in axonal domain disorganization, including the diffusion of Caspr and Cont from paranodes, and instability of the paranodal region (Pillai et al., 2009). Similarly, ablation of Cont and Caspr in mice resulted in the respective disruption of Caspr and NfascNF155, and Cont and NfascNF155, from paranodal axo-glial junctions, suggesting that these proteins may interact to coordinate and organize paranodes (Bhat et al., 2001; Boyle et al., 2001). Indeed, several studies have revealed that the localization and stabilization of both Caspr and Cont to the cell membrane is interdependent (Charles et al., 2002; Gollan et al., 2003; Bonnon et al., 2007). Specific in vitro studies have demonstrated a direct association between NfascNF155 and Cont (Gollan et al., 2003; Bonnon et al., 2007), but the true associative relationship between NfascNF155, Cont and Caspr, and how they coordinate axo-glial interactions remains unclear.

Here we report that in vivo deletion of Ig domains 5-6 in NfascNF155 mimics its complete ablation, and results in the loss of axo-glial junctions and the failure to segregate ion channels within myelinated axons. Interestingly, deletion of Ig5-6 from the neuron-specific NfascNF186 had no effect on its localization, or the overall organization of the nodes, revealing isoform specific requirements for Ig5-6 within Neurofascins. Moreover, in vitro binding assays indicate that Ig5-6 are essential for NfascNF155 interactions with Cont, but are dispensable for NfascNF186 function. Together, our results suggest that Nfasc isoforms utilize distinct protein-protein interaction modules to organize and stabilize specific axonal domains in myelinated axons.

Materials and Methods

Animals

All animal procedures were carried out under UNC-IACUC approved guidelines. The Cnp-Cre mice were provided by Dr. Klaus Nave (Max Planck Institute, Germany). β-Actin-Cre mice were obtained from the Jackson Labs (Maine, USA).

Conditional Targeting of Nfasc Gene

The strategy to delete the genomic region encoding immunoglobulin (Ig) domains 5 and 6 in the Nfasc gene was similar to that described previously (Pillai et al. 2009). In order to conditionally ablate Ig 5/6 in Nfasc locus, a targeting construct was generated in which loxP sites were targeted to flank exons 11 and 15 of Nfasc. A BAC clone (bMQ228D3) from 129 AB2.2 BAC library was obtained from the Sanger Center, England, which contained the targeted Nfasc exons. BAC recombineering technology was applied, and a polymerase II promoter directed Diphtheria toxin (DT) vector containing short PCR-generated arms homologous to that of Nfasc DNA sequence was transformed into E. coli DY380 recombinant bacterial strain (heat induced RED and GAM recombinase complex) containing the Nfasc BAC. Using this approach, a ~9.4 kb fragment of Nfasc BAC was retrieved into the DT vector by means of gap repair. The first loxP site was inserted into intron 10 and another loxP-neo-loxP cassette in intron 15. Confirmation of the functionality of the loxP sites and the proper insertion of these sites was analyzed by restriction enzyme digestion prior to embryonic stem (ES) cell targeting. The construct was then linearized and electroporated into ES cells, followed by screening of the targeted cells by PCR amplification and standard molecular biological methods for positive clones as previously described (Bhat et al. 2001).

Plasmids

The ECD-gliomedin-Fc construct was from Dr. E. Peles (Weizmann Institute), transmembrane-gliomedin construct (pcDNA3/gliomedin-TM) was from Dr. J. Devaux (CRN2M, France), HA-tagged NF186 (pEGFP-C1/HA-NF186) was from Dr. V. Bennett (Duke University, United States). NF155-Fc and NF186 deleted for Ig domain 5-6 (NF155ΔIg5-6-Fc and NF186ΔIg5-6) were generated by QuickChange mutagenesis (Stratagene, La Jolla, CA). The plasmids encoding NF155-Fc fusion (pIgplus-NF155), F3/Contactin (pRc-CMV-F3) and Caspr (pBK-CMV-pnd) have been described previously (Bonnon et al. 2007; Charles et al. 2002; Durbec et al. 1994). The primers used for creating Ig5-6 deletions were: 5′-ATGCCTTCGTCAGCGTATTACGGCCAGACC -3′, forward primer, and 5′-TAATACGCTGACGAAGGCATTGGCCAGCAGGTAGCCATG-3′, reverse primer. All mutant constructs were verified by sequencing (Cogenics, France).

Antibodies and Reagents

The following antisera were previously described: guinea pig and rabbit anti-Caspr (Bhat et al. 2001), guinea pig anti-NF186 and rat anti-pan Neurofascin (NFct) (Pillai et al. 2009). The rabbit anti-Caspr2 and rat anti-Ankyrin G (AnkG) antibodies were generated for this study. Other primary antibodies used include: mouse anti potassium channel (Kv1.1; Sigma), mouse anti-pan sodium channel (Nav pan; Sigma), rabbit anti-ezrin binding protein 50 (EBP50; Abcam), rabbit anti- neuronal cell adhesion molecule (NrCAM; Abcam), rabbit anti-Gliomedin (Glmdn; Abcam), rabbit anti-Calbindin (CalB; Sigma), rat anti-HA (Roche), rabbit anti-human Fc (Sigma), mouse anti-Human Fc (Sigma). Rabbit anti-contactin used for immunoblotting was generously provided by Dr. James Salzer (NYU, New York, USA). Additionally, for cell culture experiments, polyclonal antisera specific for F3/Contactin (pAbF3) was used as described elsewhere (Rigato et al. 2002). The rabbit anti-Gliomedin (Ab7202) was generously provided by Dr. E. Peles. Secondary antibodies used for immunofluorescence include: Alexa Fluor conjugated -488, -568, and -647 (Molecular Probes, Invitrogen), and goat anti-Human IgG (Fc specific)-TRITC (Sigma). The horse radish peroxidase (HRP)-conjugated secondary antibodies used were purchased from Jackson Immunologicals.

Tissue Preparation, Immunostaining and Immunoblotting

All tissues preparation was carried out as previously described (Bhat et al. 2001; Pillai et al. 2009). Briefly, sciatic nerves were harvested from anesthetized mice, followed by fixation in 4% paraformaldehyde (in PBS) for 15-30 min at 4°C with rocking. The nerves were washed with PBS several times and either stored at 4°C or directly teased onto slides. The slides were dried overnight at room temperature (RT) followed by immunostaining, or stored at -80°C until processed. For the preparation of spinal cord and cerebellar tissues, mice were anesthetized and transcardially perfused with ice cold 4% paraformaldehyde (in PBS). The tissues of interest were dissected out and post-fixed in 4% paraformaldehyde overnight at 4°C. The tissues were then rinsed several times in PBS, and sliced to a thickness of 30μm using a Vibratome (Leica). The sliced sections were then immediately immunostained as described previously (Pillai et al. 2009). All images from the processed tissues were acquired under identical settings using a Bio-Rad Radiance 2000 confocal microscope. The images were processed in Adobe Photoshop using identical settings between control and mutant images. Immunoblotting of protein lysates was carried out as previously described (Pillai et al., 2009).

Immunoblotting

Spinal cords from age matched wild type and mutant mice were harvested, and either directly processed or frozen at -80°C until further use. Spinal cords were suspended in ice cold homogenization buffer (50mM Tris-HCl, pH 7.5, 150mM NaCl, 320mM sucrose, 10mM EDTA, pH 8.0, and a protease inhibitor cocktail tablet). The spinal cords were homogenized on ice by 25-50 stokes using a glass mortar and pestle. The resulting homogenate was centrifuged at 1000× g for 10 min at 4°C. The supernatant was removed and subjected to further centrifugation at 13,000 rpm for 20 min at 4°C. The resulting pellet was re-suspended in extraction buffer (50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM EDTA, pH 8.0, 1% Triton X-100, and a protease inhibitor cocktail tablet) and incubated for 10-30 min at 4°C with gentle rocking. The extraction was then centrifuged at 13,000 rpm for 20 min at 4°C, and the supernatant collected. The lysate was subjected to a Lowry Assay (BC assay, BIORAD) to determine protein concentration and equal amounts were resolved on SDS-PAGE. The proteins were transferred onto nitrocellulose membranes, followed by blocking with 5% milk [dissolved in PBS with 0.1% Tween-20 (PBS-T)]. The membranes were incubated with primary antibodies for 1 hr at RT or overnight at 4°C, and were subsequently washed in PBS-T and incubated with horseradish peroxidase (HRP)- conjugated secondary antibodies for 40 mins at RT. Signals were determined by the application of chemiluminescent substrate and exposure of membranes to autoradiographic film.

Cell Culture and Transfection

The N-glycosylation mutant CHO cell line (CHO-Lec1) (Stanley and Ioffe 1995) was kindly provided by Dr. E. Fenouillet (Institut Jean Roche, Marseille, France). CHO-Lec1 and HEK-293 cells were grown in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal calf serum (FCS; Invitrogen) were transiently transfected using jetPEI (Ozyme). NF155-Fc, NF155ΔIg5-6-Fc and Gliomedin-Fc were produced in the supernatant of transfected HEK-293 cells and used for binding experiments as previously described (Charles et al. 2002; Lonigro and Devaux 2009).

Binding of Fc-Chimera and Immunofluorescence

Cells transfected with Cont, Cont + Caspr, Gliomedin or NF186 were incubated with NF155-Fc, NF155ΔIg5/6-Fc or Gliomedin-Fc (5 μg/ml) for 30 min at 37°C, washed, and then incubated with TRITC-conjugated anti-Human Fc prior to fixation with 4% paraformaldehyde. For surface immunostaining of HA-tagged constructs, fixed cells were incubated with rat anti-HA antibody followed by fluorescently-labeled secondary antibodies diluted in PBS containing 3% bovine serum albumin (BSA). After washing in PBS, the cells were mounted in Mowiol (Calbochiem, La Jolla, CA) and examined using an apotome AxioObserver Z1 inverted microscope under the control of Axiovision software (Carl Zeiss, Jena, Germany).

Protein Pull-down Assay

CHO-Lec1 cells transiently transfected with Contactin were homogenized on ice with lysis buffer (containing 60mM octyl glucoside and 1% TritonX-100) as previously described (Charles et al. 2002). Solubilized proteins (1.5mg per condition) were incubated with Fc fusion proteins (100μg) immobilized onto protein A-Sepharose CL-4B beads (200μl, GE Healthcare, Orsay, France). Affinity retained proteins were analyzed by immunoblotting as described previously (Bonnon et al. 2007).

Transmission Electron Microscopy (TEM)

TEM of age-matched wild type, Cnp-Cre;NfascIgFlox, β-Act-Cre;NfascIgFlox mutant mice was carried out essentially as described previously (Pillai et al. 2009).

Additional experimental procedures are provided in the “Supplementary Materials and Methods” section.

Results

Targeted Deletion of Ig Domains 5 and 6 in Neurofascin

We recently demonstrated that the myelinating glia-specific isoform of Neurofascin (NfascNF155) is required for the formation and maintenance of paranodal axo-glial junctions (Pillai et al., 2009). To further dissect the role of NfascNF155 in paranodal organization, we generated mice specifically lacking Ig domains 5 and 6 (Ig5-6) from Nfasc (Fig.1A). These two domains represent the mid-point of the extracellular region in both the glial and neuronal isoforms of Nfasc, and are precisely encoded by exons 11-15 (http://www.ensembl.org/Mus_musculus). A targeting construct where exons 11 and 15 were flanked by loxP sites (Fig. 1B, red arrows) was used to generate NfascIgFlox mice. The genotypes of the wild-type (+/+), heterozygous (+/NfascIgFlox), and homozygous (NfascIgFlox) mice were identified by PCR using specific primers (Fig. 1C). Next, we determined whether loss of Ig5-6 from NfascNF155, using Cnp-Cre (Lappe-Siefke et al., 2003), resulted in an analogous phenotype as complete ablation of NfascNF155. Examination of the Cnp-Cre;NfascIgFlox mice revealed phenotypes consistent with that observed in NfascNF155 null mice including severe ataxia, motor coordination defects, and progressive weight loss (Fig.1D and Supplemental (S) Fig.1) (Pillai et al., 2009). To confirm isoform specific deletion of Ig5-6 in NfascNF155 and NfascNF186, immunoblot analysis was carried out on wild-type (+/+), NfascNF155ΔIg5-6 (Cnp-Cre;NfascIgFlox, Fig.1E) and NfascΔIg5-6 (β-Act-Cre;NfascIgFlox, Fig.1F) spinal cords using a pan-Neurofascin (NFct) antibody (Pillai et al., 2009). In wild-type (+/+) spinal cord lysate, nodal NfascNF186 and glial NfascNF155 were observed at their respective molecular weights. In both Cnp-Cre;NfascIgFlox and β-Act-Cre;NfascIgFlox lysates, a 135kDa band corresponding to loss of Ig5-6 from NfascNF155 was observed (pink asterisks), and indicates that excision of Ig5-6 did not affect translation of the mutant protein. Additionally, a 166kDa band corresponding to deletion of Ig5-6 from NfascNF186 was observed in heterozygous (+/β-Act-Cre;NfascIgFlox) and β-Act-Cre;NfascIgFlox lysates compared to wild-type (Fig.1F, pink asterisks). Caspr and Cont showed no changes between wild-type, Cnp-Cre;NfascIgFlox, and β-Act-Cre;NfascIgFlox lysates. Together, these results demonstrate the successful ablation of Ig5-6 in Nfasc and reveal that loss of Ig5-6 in NfascNF155 phenocopies its complete ablation; furthermore, they highlight an essential role for these domains in the organization of paranodes by NfascNF155.

Figure 1
Genetic Ablation of Ig5-6 from Nfasc

Ig5-6 of NfascNF155, but not NfascNF186, Are Essential for Axonal Domain Organization in Myelinated Axons

To examine whether loss of Ig5-6 in NfascNF155 effected paranodal formation and axonal domain organization, we immunostained sciatic nerve fibers with antibodies against key juxtaparanodal, paranodal, and nodal molecules (Fig.2A-H). Immunoreactivity of the pan-Neurofascin (NFct) antibody (green) revealed specific loss of NfascNF155 from the paranodes in Cnp-Cre;NfascIgFlox nerves compared to wild-type (+/+) nerves, whereas the localization of nodal NfascNF186 (NF186, red) was unchanged (Fig.2A-B). Further examination of the nerve fibers revealed the presence of the mutant NfascNF155 protein within Schmidt-Lantermann incisures in Cnp-Cre;NfascIgFlox fibers (Fig.2B′), as in wild-type. In addition, NF155 (green) was observed in a small number of paranodes (arrowheads) in Cnp-Cre;NfascIgFlox nerves (Fig.2D-D′) as in wild-type (Fig.2C-C′) suggesting that loss of Ig5-6 did not affect the transport or the ability of NfascNF155ΔIg5-6 to localize to the paranodes or within the glial plasma membrane. In contrast, enriched paranodal Caspr (blue) fluorescence was not observed in Cnp-Cre;NfascIgFlox fibers, but was rather diffused throughout the internode (Fig.2F-F[triple prime]) compared to wild-type nerves (Fig.2E-E[triple prime]). In addition, juxtaparanodal shaker-type potassium channels (Kv1.1, red) redistributed into the paranodal space in Cnp-Cre;NfascIgFlox fibers, and directly abutted the node (NF186, green). Accordingly, juxtaparanodal Caspr2 was also found mislocalized in the paranodes in Cnp-Cre;NfascIgFlox nerves compared to wild-type nerves (SFig.2). Interestingly, even in the absence of proper paranodal formation, as observed in Cnp-Cre;NfascIgFlox mutant nerves, both ankyrinG (AnkG, red) and NfascNF186 (NF186, blue) remained localized to nodes (Fig. 2H-H[triple prime]). Together, these findings further demonstrate that deletion of Ig5-6 in NfascNF155 phenocopies its complete ablation, and suggest that these domains are essential for NfascNF155 function in organizing the paranodes.

Figure 2
Loss of Ig5-6 in NfascNF155 Results in Axonal Domain Disorganization

Due to the significant homology between the Ig domains of NfascNF155 and NfascNF186 (Fig.1A) (Davis et al., 1996), and the importance of Ig5-6 to NfascNF155 function, we sought to determine whether these domains were equally indispensable for NfascNF186 function and localization at nodes. To ablate Ig5-6 from NfascNF186, the NfascIgFlox mice were crossed with the ubiquitously expressed Cre line, β-Actin-Cre (β-Act-Cre), which resulted in the excision of Ig5-6 from both isoforms of Nfasc (Fig.1F). To assess axonal domain organization, we immunostained teased sciatic nerve fibers from wild-type (+/+) and NfascΔIg5-6 (β-Act-Cre;NfascIgFlox) mice with the indicated antibodies (Fig. 2I-N). As expected, Caspr (green) localization to paranodes was disrupted in β-Act-Cre;NfascIgFlox fibers (Fig.2J-J[triple prime]) compared to wild-type (+/+) fibers (Fig.2I-I[triple prime]). In addition, we found that juxtaparanodal Kv1.1 were mislocalized in β-Act-Cre;NfascIgFlox mice, as in Cnp-Cre;NfascIgFlox, consistent with loss of Ig5-6 from NfascNF155 (SFig.2). Interestingly, voltage-gated sodium channels (Navpan, red) remained enriched at nodes, and colocalized with NF186 (blue) in β-Act-Cre;NfascIgFlox nerves as in wild-type. AnkG localization to nodes was also unaffected in β-Act-Cre;NfascIgFlox (SFig.2). These findings suggest that loss of Ig5-6 does not disrupt the ability of NfascNF186 to associate with, or organize other axonally-expressed nodal components.

In the PNS, the Schwann cell microvilli overlie the node and express gliomedin (Glmdn), ezrin binding phosphoprotein 50 (EBP50) and phosphorylated ezrin/radixin/moesin (ERM) proteins (Eshed et al., 2005; Melendez-Vasquez et al., 2001). Due to their importance in PNS nodal development, we sought to determine whether loss of Ig5-6 in NfascNF186 affected the expression and/or localization of EBP50 and Glmdn by immunostaining (Fig.2K-N). In both wild-type and β-Act-Cre;NfascIgFlox myelinated fibers, EBP50 (green) colocalized with NfascNF186 (NFct, blue) at nodes (Fig.2K-L). Accordingly, Glmdn (green) remained localized at nodes in β-Act-Cre;NfascIgFlox nerves, as in wild-type, where it also colocalized with NfascNF186 (blue, NFct; Fig.2N-N[triple prime]). These results suggest that Ig5-6 of NfascNF186 are not responsible for the association, organization, or stabilization of the microvilli or its related proteins to the node.

We further analyzed axonal domain organization in CNS myelinated fibers of wild-type (+/+) and Cnp-Cre;NfascIgFlox mice (Fig.2O-R). As in the PNS, juxtaparanodal Kv1.1 (red) channels were mislocalized, and Caspr (blue) was diffused in Cnp-Cre;NfascIgFlox white matter (Fig.2P-P[triple prime]), while NF186 (green) was concentrated at the nodes. In addition, AnkG (red) was properly localized at CNS nodes in Cnp-Cre;NfascIgFlox spinal cords, where it colocalized with NF186 (blue) regardless of paranodal Caspr (green) (Fig.2R). Similar results were observed in the CNS of β-Act-Cre;NfascIgFlox mice (SFig.2). Overall, these results suggest that Ig5-6 are dispensable for NfascNF186 function in localizing, stabilizing, and organizing the node, but are critical for NfascNF155 function in forming and maintaining the paranodes.

Paranodal Septate Junction Formation and Organization is Disrupted in NfascNF155Δ Ig5-6 Mutant Mice

Due to the analogous phenotypes observed between complete loss of NfascNF155 and NfascNF155ΔIg5-6 at the light microscopic level, we used electron microscopic analysis to characterize the anatomical organization of myelinated fibers in NfascNF155ΔIg5-6 mice (Fig.3). Specific attention was focused on the septate-like junctions formed between paranodal loops and the axon. These junctions appear as ladder-like electron dense transverse bands in the paranodal space. Low magnification micrographs of wild-type (+/+; Fig.3A) and Cnp-Cre;NfascIgFlox nerve fibers (Fig.3B) appeared similar, except for the presence of organelles within the node(s) of Cnp-Cre;NfascIgFlox mice. This accumulation is consistent with previous findings observed in mice with disrupted paranodal axo-glial junctions (Einheber et al., 2006; Garcia-Fresco et al., 2006; Pillai et al., 2007). Closer examination revealed loss of septae in the paranodal region of Cnp-Cre;NfascIgFlox nerves (Fig.3D) compared to the wild-type fibers where the septae are clearly visible (Fig.3C). Concomitant with the loss of septae, organelle accumulation was also observed in Cnp-Cre;NfascIgFlox nerves (Fig.3F, arrows) compared to wild-type (+/+, Fig.3E). A similar loss of paranodal septae was observed in myelinated CNS spinal cord fibers in Cnp-Cre;NfascIgFlox mice (Fig.3H) compared to wild-type (Fig.3G, arrowheads). The paranodal loops were often everted (EL) away from the axon and astrocytic processes (asterisks) infiltrated the paranodal space in the CNS of Cnp-Cre;NfascIgFlox mice (Fig.3I and J). Moreover, the parallel arrays of cytoskeletal neurofilaments were disrupted in Cnp-Cre;NfascIgFlox CNS fibers (Fig. 3L, arrowheads). Similarly, cross sections of cerebellar white matter from Cnp-Cre;NfascIgFlox mice (Fig. 3N) revealed the presence of degenerating vacuoles within axons (asterisks), compared to wild-type (Fig.3M, asterisks). We also observed the presence of swellings in Purkinje neuron axons of Cnp-Cre;NfascIgFlox mice similar to Cnp-Cre;NfascFlox mice (SFig.3) (Pillai et al., 2009). Together, these results reveal an important role for Ig5-6 in the ability of NfascNF155 to coordinate and/or form paranodal septate junctions.

Figure 3
NfascNF155ΔIg5-6 Mutant Mice Fail to Establish Paranodal Axo-Glial Junctions

Ig5-6 Are Essential for the Association of Neurofascins with Contactin, but not with Gliomedin

Several reports have suggested a possible interaction between NfascNF155 and Cont/Caspr within the paranodal domains of myelinated fibers, but the exact mechanisms governing these interactions remain unclear (Charles et al., 2002; Gollan et al., 2003; Bonnon et al., 2007). Previously, we reported that NfascNF155 preferentially bound a high-mannose, N-glycosylated form of Cont in vitro (Bonnon et al., 2007). Here, we assessed whether Ig5-6 are critical for the association of NfascNF155 with Cont and/or Caspr, and NfascNF186 with Glmdn (Fig.4). First, Fc-tagged constructs of wild-type NfascNF155 (NF155-Fc), NfascNF155 with deleted Ig5- 6 (NF155ΔIg5/6-Fc), and Glmdn (Glmdn-Fc), and HA-tagged constructs of wild-type NfascNF186 (NF186-HA) and NfascNF186 lacking Ig5-6 (NF186ΔIg5/6-HA) were produced and expressed in HEK-293 (Fig.4A) and CHO-Lec1 cells (Fig.4B), respectively. The deletion of Ig domains 5-6 encompassed amino acids 414-597 of Nfasc and was identical to the targeting construct used to develop the NfascIgFlox mice. Next, we performed pull-down assays using the Fc-tagged NfascNF155 proteins against lysates from CHO-Lec1 cells over-expressing Cont (Fig. 4C), or rat brain lysates (Fig. 4D) endogenously expressing Cont and Caspr. While wild-type NfascNF155 (WT-FC) precipitated Cont, and Cont+Caspr from CHO-Lec1 cell and rat brain lysates respectively, the ability of NfascNF155 lacking Ig5-6 (ΔIg-Fc) to precipitate Cont and/or Caspr from cell lysates was dramatically reduced. Total extracts from cell lysates showed that equal levels of Cont, Caspr and the Fc-tagged proteins were expressed, therefore, these findings indicate that Ig5-6 are essential for the interaction of NfascNF155 with Cont.

Figure 4
Ig5-6 Are Essential for NfascNF155 Association with Cont

Next we performed in vitro binding assays where CHO-Lec1 cells transiently expressing Cont, Cont+Caspr, or Glmdn were exposed to the different Fc-Tagged NfascNF155 proteins. Similar to the pull down assays, NF155ΔIg5/6-Fc was not able to stably associate with CHO-Lec1 cells expressing Cont, or Cont+Caspr (Fig.4F), compared to wild-type NfascNF155 (NF155-Fc; Fig.4E). Interestingly, loss of Ig5-6 from NfascNF155 did not affect its ability to bind Glmdn. This result is of no major consequence, as to date, no functional relationship between these two proteins has been characterized. However, Glmdn can interact with NfascNF186 in vitro, and is proposed to act in facilitating nodal organization in the PNS (Eshed et al., 2005). Therefore, we sought to determine whether Ig5-6 of NfascNF186 are critical for its interaction with Glmdn in vitro. HA-tagged NfascNF186 (NF186 WT-HA) and NfascNF186 deleted for Ig5-6 (NF186ΔIg5/6-HA) both localized to the plasma membranes of transiently expressing CHO-Lec1 cells (Fig. 4G). When exposed to Fc-tagged Glmdn, both NF186-HA and NF186ΔIg5-6-HA transiently expressing CHO-Lec1 cells bound Glmdn (Fig.4G). This data is consistent with the observation that loss of Ig5-6 in NfascNF186 did not affect the recruitment or expression of Glmdn to PNS nodes (Fig. 2N″). Taken together, these results demonstrate that Ig5-6 are indispensable for the stable association of NfascNF155 with the paranodal component Cont, and is the first mechanistic evidence identifying critical domains within Nfasc that are required for its proper functioning in establishing the paranodal axo-glial junctions.

Discussion

Recent findings have advanced our knowledge of key molecules regulating myelination, and more specifically axonal domain organization, but little is known of how these domains become organized during nervous system development. Here, using mutational analysis, we find that Ig5-6 of NfascNF155, but not NfascNF186, are essential for axonal domain organization and integrity by facilitating the stable association of NfascNF155 with Cont, both in vivo and in vitro. These studies are the first to reveal domain specific requirements for paranodal axon-glial interactions, and therefore, may provide valuable clues to the mechanisms leading to disorganization of axonal domains during disease onset and progression.

Ig Domain Perturbation Resembles Complete Ablation of NfascNF155

Mice lacking NfascNF155 expression presented with severe motor coordination defects (Pillai et al., 2009) similar to Caspr and Cont mutant mice, and survived to postnatal day 17 (Bhat et al., 2001; Boyle et al., 2001). Here, we find that loss of Ig5-6 alone from NfascNF155 in mice, completely recapitulates the phenotypes of NfascNF155 null mice, including the onset of paralysis and ataxia, paranodal disorganization, and loss of paranodal septae. These findings suggest that Ig5-6 may act to coordinate Cont or Cont/Caspr localization to the paranodes during myelination. When closely examined, we found that Cnp-Cre;NfascIgFlox myelinated nerves expressed the mutant NfascNF155ΔIg5-6 protein within the Schmidt-Lantermann incisures (Fig. 2B) and the paranodes (Fig.2D) demonstrating that loss of Ig5-6 does not perturb the ability of NfascNF155 to localize to the paranodal plasma membrane. Therefore, it is likely that these domains are responsible for promoting the association of NfascNF155 with Cont and/or Caspr, thereby stabilizing the paranodal complex. Together, these results show that Ig5-6 are critical for the stabilization of the paranodal axo-glial junctions.

Association of NfascNF155 with Cont/Caspr Complex is Mediated by Ig5-6

The analogous paranodal deformities observed between NfascNF155, NfascNF155ΔIg5-6, Caspr and Cont mutant mice supports the hypothesis that these proteins may interact in a complex to facilitate axonal domain organization in myelinated fibers as is observed in invertebrate septate junction formation (Faivre-Sarrailh et al., 2004; Banerjee and Bhat, 2008). Initial vertebrate studies performed in vitro demonstrated that the extracellular domain of NfascNF155 was capable of associating with the Cont/Caspr complex (Charles et al., 2002). Yet, another study suggested that Caspr perturbd the association of Cont and NfascNF155 in vitro, thus the true mechanism of these interactions remains to be established (Gollan et al., 2003). To address this issue, we previously showed, in vitro, that NfascNF155 associated with a glycoslated form of Cont in the presence or absence of Caspr (Bonnon et al., 2007). Here, we find that Ig5-6 are critical for the interaction of NfascNF155 with Cont. In vitro pull down assays revealed a dramatic reduction in the ability of NfascNF155 to associate with Cont, or Cont+Caspr, in the absence of Ig5-6 (Fig.4C-D). Accordingly, cell binding assays indicated that Fc-tagged NfascNF155 lacking Ig5-6 was not able to stably adhere to cells over-expressing Cont, or Cont+Caspr, and thus coincide with our in vivo data showing diffuse distribution of Caspr and Cont in NfascNF155ΔIg5-6 myelinated axons. Interestingly, NfascNF155 was able to associate with Glmdn regardless of the presence of Ig5-6, indicating that ablation of these domains does not significantly alter the protein structure or protein folding of NfascNF155. Overall, these results provide evidence supporting a role for NfascNF155 in coordinating paranodal axo-glial junction formation, organization, and stabilization by binding Cont, and facilitating complex formation with Caspr.

Domain Specific Requirements for the Function of Neurofascins During Nervous System Development

To date, up to fifty alternatively spliced variants of the Nfasc gene have been identified in chicken, but only two major murine isoforms have been characterized as critical regulators of nervous system development, glial-specific NfascNF155 and neuronal-specific NfascNF186 (Koticha et al., 2006; Hassel et al., 1997; Davis et al., 1996). Due to the high percentage of homology in the extracellular domains of NfascNF155 and NfascNF186, it is plausible that they function equivalently to organize and stabilize their paranodes and nodes, respectively. By using β-Actin-Cre, we ablated Ig5-6 from NfascNF155 and NfascNF186. Concomitant loss of Ig5-6 from both isoforms led to paranodal disruption identical to that observed in mice deficient of Ig5-6 in NfascNF155 alone. Interestingly, ablation of Ig5-6 from NfascNF186 did not result in nodal disorganization, as NfascNF186, along with several other major nodal components localized to nodes, even in the absence of intact paranodes (Fig.2). Furthermore, electrophysiological data revealed no change in conduction velocity between Cnp-Cre;NfascIgFlox mice and β-Act-Cre;NfascIgFlox mice (SFig.4). If, indeed, the Ig domains were essential for NfascNF186 function, one would have expected a more profound or cumulative decline in nerve conduction velocity in β-Act-Cre;NfascIgFlox mice compared to Cnp-Cre;NfascIgFlox mice. Additionally, the in vitro binding assays revealed that NfascNF186 maintained its ability to associate with Glmdn in the absence of Ig5-6. Taken together, these results demonstrate that individual isoforms of Nfasc have distinct domain requirements regulating their ability to interact with, and organize regional specific components within myelinated axons.

In summary, we have provided evidence of specific domains within NfascNF155 that are critical for paranodal formation, and overall axonal domain organization and integrity. Perturbation of these domains resulted in severe paranodal abnormalities, disorganized axonal domains and significant nerve conduction delays. Future in vivo structure/function studies, aimed at identifying critical regions of protein-protein interactions between key molecular players coordinating domain organization, will be invaluable in elucidating the mechanisms underlying neuron-glia interactions in myelinated axons.

Supplementary Material

Supp1

Acknowledgments

We are grateful to Dr. Klaus-Armin Nave (Max Planck Institute of Experimental Medicine, Germany) for generously sharing the Cnp-Cre mouse strain. We thank Dr. E. Perl for his generous support and advice with electrophysiology. We also thank Dr. A. Fanning and our fellow lab members for critical discussions and comments. This work was supported by NIH grants NS050356 and GM063074 (MAB), and National Multiple Sclerosis Society grants (MAB and CFS).

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