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J Bacteriol. 1973 Nov; 116(2): 803–811.
PMCID: PMC285449

Effect of the Prophage and Penicillinase Plasmid of the Recipient Strain Upon the Transduction and the Stability of Methicillin Resistance in Staphylococcus aureus

Abstract

Transduction of a methicillin-resistance determinant (mec) in Staphylococcus aureus RN450 was dependent on its prior lysogenization with an appropriate temperate phage. In addition, an appropriate transduced penicillinase plasmid was usually required. Some phage 80-resistant variants of RN450 or of its parental lysogenic strain, NCTC 8325, were also effective recipients for transduction of mec. Elimination of prophage from RN450 abrogated its effectiveness as a transductional recipient of mec. Elimination of prophage from a methicillin-resistant transductant of RN450 reduced resistance to undetectable levels in six of seven phage-eliminated strains. In four of these a variable number of clones again became phenotypically resistant after lysogenization alone or lysogenization combined with reintroduction of a penicillinase plasmid. In two prophage-eliminated strains, no evidence of residual mec could be adduced. The establishment, expression, or stability of the transduced mec in strain RN450 appeared to depend on some function determined by a prophage or a prophage and a penicillinase plasmid.

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Selected References

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  • al-Salihy SM, James AM. Loss of methicillin-resistance from resistant strains of Staphylococcus aureus. Lancet. 1972 Aug 12;2(7772):331–332. [PubMed]
  • Annear DI, Grubb WB. Spontaneous loss of methicillin resistance in Staphylococcus aureus. Lancet. 1973 Jan 13;1(7794):110–110. [PubMed]
  • ARBER W. Transduction of chromosomal genes and episomes in Escherichia coli. Virology. 1960 May;11:273–288. [PubMed]
  • Bouanchaud DH, Scavizzi MR, Chabbert YA. Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. J Gen Microbiol. 1968 Dec;54(3):417–425. [PubMed]
  • Chabbert YA, Baudens JG, Acar JF, Gerbaud GR. La résistance naturelle des staphylocoques à la méthicillin et l'oxacilline. Rev Fr Etud Clin Biol. 1965 May;10(5):495–506. [PubMed]
  • Cohen S, Sweeney HM. Transduction of Methicillin Resistance in Staphylococcus aureus Dependent on an Unusual Specificity of the Recipient Strain. J Bacteriol. 1970 Dec;104(3):1158–1167. [PMC free article] [PubMed]
  • Dornbusch K, Hallander HO, Löfquist F. Extrachromosomal control of methicillin resistance and toxin production in Staphylococcus aureus. J Bacteriol. 1969 May;98(2):351–358. [PMC free article] [PubMed]
  • Gagliano VJ, Hinsdill RD. Characterization of a Staphylococcus aureus bacteriocin. J Bacteriol. 1970 Oct;104(1):117–125. [PMC free article] [PubMed]
  • GRAVENKEMPER CF, BRODIE JL, KIRBY WM. RESISTANCE OF COAGULASE-POSITIVE STAPHYLOCOCCI TO METHICILLIN AND OXACILLIN. J Bacteriol. 1965 Apr;89:1005–1010. [PMC free article] [PubMed]
  • Grubb WB, Annear DI. Spontaneous loss of methicillin resistance in Staphylococcus aureus at room-temperature. Lancet. 1972 Dec 9;2(7789):1257–1257. [PubMed]
  • JACOB F, WOLLMAN EL. Etude génétique d'un bactériophage tempéré d'Escherichia coli. III. Effet du rayonnement ultraviolet sur la recombinaison génétique. Ann Inst Pasteur (Paris) 1955 Jun;88(6):724–749. [PubMed]
  • Lacey RW. Genetic control in methicillin-resistant strains of Staphylococcus aureus. J Med Microbiol. 1972 Nov;5(4):497–508. [PubMed]
  • NOVICK RP. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. [PubMed]
  • Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. [PubMed]
  • Novick RP, Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. [PubMed]
  • Novick RP, Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. [PMC free article] [PubMed]
  • Peyru G, Wexler LF, Novick RP. Naturally occurring penicillinase plasmids in Staphylococcus aureus. J Bacteriol. 1969 Apr;98(1):215–221. [PMC free article] [PubMed]
  • Sjöström JE, Lindberg M, Philipson L. Competence for transfection in Staphylococcus aureus. J Bacteriol. 1973 Feb;113(2):576–585. [PMC free article] [PubMed]
  • Smith K, Novick RP. Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus. J Bacteriol. 1972 Nov;112(2):761–772. [PMC free article] [PubMed]
  • Tessman I. Selective stimulation of one of the mechanisms for genetic recombination of bacteriophage S13. Science. 1968 Aug 2;161(3840):481–482. [PubMed]
  • Yoshikawa M, Hirota Y. Impaired transduction of R213 and its recovery by a homologous resident R factor. J Bacteriol. 1971 May;106(2):523–528. [PMC free article] [PubMed]

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