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Journal of Neurotrauma
J Neurotrauma. Aug 2009; 26(8): 1369–1378.
PMCID: PMC2850290
NIHMSID: NIHMS139580

Temporal and Spatial Dynamics of Peroxynitrite-Induced Oxidative Damage after Spinal Cord Contusion Injury

Abstract

The reactive nitrogen species peroxynitrite (PN) has been suggested to be an important mediator of the secondary oxidative damage that occurs following acute spinal cord injury (SCI). The PN decomposition products nitrogen dioxide (•NO2), hydroxyl radical (•OH), and carbonate radical (•CO3) are highly reactive with cellular lipids and proteins. In this immunohistochemical study, we examined the temporal (3, 24, and 72 h, and 1 and 2 weeks) and spatial relationships of PN-mediated oxidative damage in the contusion-injured rat thoracic spinal cord (IH device, 200 kdyn, T10) using 3-nitrotyrosine (3-NT), a marker for protein nitration by PN-derived •NO2 and 4-hydroxynonenal (4-HNE), an indicator of lipid peroxidation (LP) initiated by any of the PN radicals. Minimal 3-NT or 4-HNE immunostaining was seen in sham, non-injured spinal cords. In contrast, both markers showed a substantial increase at 3 h post-injury at the epicenter, that extended throughout the gray matter and into the surrounding white matter. At 24 and 72 h, the oxidative damage expanded circumferentially to involve all but a small rim of white matter tissue at the injury site, and longitudinally as much as 6–9 mm in the rostral and caudal directions. The staining was observed in neuronal soma, axons, and microvessels. At all time points except 3 h, there was no significant difference in the mean rostral or caudal extent of 3-NT and 4-HNE staining. By 1, and more so at 2 weeks, the longitudinal extent of the oxidative damage staining was greatly decreased. The spatial and temporal overlap of 3-NT and 4-HNE staining supports the concept that PN is involved in both damage produced by lipid peroxidation and protein nitration, and that antioxidant agents that target PN or PN-derived radicals should be effective neuroprotectants for acute SCI if administered during the first post-injury hours.

Key words: 4-hydroxynonenal, lipid peroxidation, peroxynitrite, protein nitration, spinal cord injury, 3-nitrotyrosine

Introduction

The role of reactive oxygen-induced oxidative damage to spinal cord lipids (i.e., lipid peroxidation, LP) and proteins following acute spinal cord injury (SCI) has been strongly supported in previous work (Aksenova et al., 2002; Baldwin et al., 1998; Braughler and Hall, 1989; Hall and Braughler, 1993; Juurlink and Paterson, 1998; Springer et al., 1997). Earlier investigations attributed much of the post-traumatic oxidative damage to the reactive oxygen species (ROS) hydrogen peroxide (H2O2) and its derived hydroxyl radical (•OH) formed rapidly by iron-dependent Fenton reactions (Braughler and Hall, 1989). However, Beckman and coworkers (Beckman et al., 1990; Beckman, 1991) subsequently introduced the theory that another ROS that could logically be involved in producing secondary oxidative tissue injury was peroxynitrite (PN). Peroxynitrite, often referred to as a reactive nitrogen species, is formed by the diffusion rate–limited reaction of nitric oxide synthase (NOS)-generated nitric oxide (•NO) with superoxide radicals (O2•−) at diffusion-limited rates to form PN anion (ONOO) (Radi, 2004). However, the potent oxidizing ability of PN is actually due to its free radical decomposition products. These products are formed in two pathways. The first involves the protonation of PN to form peroxynitrous acid (ONOOH), which can undergo homolytic decomposition to form the highly reactive nitrogen dioxide radical (•NO2) and hydroxyl radical (•OH) (Beckman et al., 1990; Beckman, 2002; Radi et al., 2002b). In addition, PN will react with carbon dioxide (CO2) to form nitrosoperoxocarbonate (ONOOCO2), which can then decompose into •NO2 and carbonate radical (CO3•−) (Deng et al., 2007; Radi, 2004; Squadrito and Pryor, 2002; Xiong et al., 2007). These radicals can initiate cellular damage by LP by attacking polyunsaturated fatty acids (Radi et al., 1991). The LP by-product 4-hydroxynonenal (4-HNE), as well as other aldehydes formed from peroxidized lipids can then bind to cellular proteins compromising their structural and functional integrity (Halliwell and Gutteridge, 2007). Additionally, •NO2 can nitrate the 3 position of tyrosine residues in proteins (Radi et al., 2002a; Radi, 2004). Accordingly, the measurement of 3-nitrotyrosine (3-NT) accumulation thus provides a biomarker of PN action (Beckman and Koppenol, 1996; Radi et al., 1991; Radi, 2004).

Prior studies in other laboratories (Liu et al., 2000; Scott et al., 1999) and our own (Xiong et al., 2007) have demonstrated 3-NT immunostaining in the injured spinal cord. Furthermore, recent work in our laboratory has provided convincing evidence for an important role of PN in the oxidative damage that occurs in the injured spinal cord and its relationship to other secondary injury events including intracellular calcium overload, mitochondrial dysfunction, and calpain-mediated cytoskeletal protein degradation (Xiong et al., 2007). The goal of the present experiments was to provide a more complete immunohistochemical definition of the time course, intensity and rostral-caudal extent of LP (4-HNE) and protein nitration (3-NT) in the contused rat spinal cord. The purpose was to more convincingly investigate the spatial and temporal overlap of the two oxidative injury mechanisms in order to support the hypothesis that PN is a major source of both in the injured spinal cord, and that PN-mediated damage affects both microvascular and parenchymal elements. The results would then enable the more rational design of PN-targeted antioxidant neuroprotective strategies.

Methods

Subjects

These experiments employed 24 young adult female Sprague-Dawley rats (Charles River, Portage, MI) weighing between 200 and 225 g. The animals were randomly cycling and were not tested for stage of the estrus cycle. They were fed and watered ad libitum. All procedures described below have been approved by the University of Kentucky Institutional Animal Care and Use Committee, and followed NIH Guidelines for the Care and Use of Laboratory Animals.

Rat contusion model of spinal cord injury

The rats were anesthetized with ketamine (80 mg/kg IP) and xylazine (10 mg/kg IP). A laminectomy slightly larger than the 2.5-mm impactor tip was made at the T10 vertebra and the vertebral column was stabilized by clamping the rostral T9 and caudal T11 vertebrae with forceps. The exposed cord was then manipulated to be level in the horizontal plane. A spinal cord contusion injury was then performed using the Infinite Horizons injury device (Scheff et al., 2003). This device applied a force-defined impact to the exposed spinal cord with a 2.5-mm-diameter stainless steel–tipped impactor producing a reproducible contusion injury. The injury force used for this experiment was 200 kdyn, which produces a severe degree of contusion injury. A computer monitored the impact and recorded velocity and actual force of the impact, as well as the displacement of the spinal cord, allowing us to standardize the level of injury across all animals. Sham rats received the laminectomy but were not injured.

After injury, all rats received 20 mL of sterile lactated Ringer's solution injected SC bilaterally (10 mL per side) just rostral to the hind limbs to prevent dehydration, as well as 33.3 mg/kg of cefazolin also injected SC above the tail. They were then placed on 37° water-circulating heating pads and returned to the animal room. The animals were housed two per cage until sacrifice, with postoperative animal care consisting of manual bladder expression, injection of 20 mL of lactated Ringer's solution, and injection of 33.3 mg/kg of cefazolin being performed twice daily until sacrifice or until 1 week post-injury. Any animal that showed signs of distress was also given 0.035 mg/kg of buprenorphine IM.

Tissue processing

At various time points after injury (3, 24, and 72 h, and 1 or 2 weeks) the rats (n = 4/time point) were deeply anesthetized with sodium pentobarbital (150 mg/kg IP) and transcardially perfused with 150 mL of 0.1 M PBS followed by 200 mL of 4% paraformaldehyde. Four sham (laminectomized but non-injured) rats were also perfused at 24 h post-surgery. The spinal cord from the base of the skull to the cauda equina was then removed from the vertebral column and placed in 4% paraformaldehyde for 4 h before being transferred to 0.2 M PBS for 1 to 5 days. Two days before being embedded in OTC (Tissue-Tek; Sakura Finetek USA, Inc., Torrance, CA), the cords were cryoprotected in 20% sucrose in 0.2 M PBS. The tissue was cut into four sections as follows: (a) 3 cm centered on the injury epicenter, (b) 3 cm spanning 4.5 to 1.5 cm from the injury epicenter, (c) the remainder of the cord 4.5 cm rostral to the injury epicenter, and (d) the remainder of the cord 1.5 cm caudal to the injury epicenter. The excised cord sections were stored at −80°C for several days. The spinal cords were sectioned in the transverse plane at 20 μm thickness and every fifth section was placed directly onto a series of 10 Superfrost Plus Slides (Fisher Scientific, Hampton, NH). The slides were dehydrated overnight at 4°C before being stored at −20°C until staining.

Immunohistochemical analysis of 4-HNE and 3-NT

The day of staining, three adjacent series of slides were thawed and stained for 4-HNE, 3-NT, and no-primary controls. Those being probed for 4-HNE were subjected to a reduction protocol using 0.1 M NaBH4 in 0.1 M MOPS (Sigma-Aldrich, St. Louis, MO). All slides were then incubated in 0.3% H2O2 in 0.2 M PBS for 30 min and blocked in 5% normal goat serum, 0.25% Triton X-100, and 1% dry milk in 0.2 M PBS for 2 h at room temperature. The slides were then incubated in the same blocking solution containing either anti-4-hydroxynonenal (1:5000, rabbit anti-HNE Michael Adducts; EMD Chemicals, Gibbstown, NJ) or anti-nitrotyrosine (1:2000, rabbit anti-nitrotyrosine; Millipore Corp., Temecula, CA) antibodies overnight at 4°C. The no-primary controls were incubated in the blocking solution only. The next day, the tissue was placed in blocking solution containing biotinylated goat anti-rabbit secondary antibody (1 drop/20 mL, Vectastain ABC-AP kit; Vector Laboratories, Burlingame, CA) for 2 h at room temperature before being incubated in Vectastain ABC reagent (1 drop/5 mL, Vectastain ABC-AP kit; Vector Laboratories) for 1 h. Staining was then visualized using the Vector Blue substrate kit (Vector Laboratories). Spinal cord sections were lightly counterstained with nuclear fast red (Vector Laboratories) and cover slipped with Permount.

Photomicrographs were taken using an Olympus Magnafire digital camera mounted on an Olympus Provis AX70 microscope (Olympus, Center Valley, PA) at either 4×, 20×, or 40× magnification. To semi-quantitatively determine the anterior-posterior extent of the post-traumatic oxidative damage (4-HNE and 3-NT), all sections were examined for the presence of staining. A section was considered to indicate the presence of either marker if staining was present above the level found in the corresponding section from the sham, non-injured rats. Since only every fifth 20-μm section was stained, the rostral or caudal extent of the oxidative damage staining was determined by multiplying the number of successively stained sections by 100 μm. Accordingly, the rostral and caudal extents of 4-HNE and 3-NT staining were calculated for each animal (n = 4 for each time-point), and the mean ± standard error calculated for each post-traumatic time-point. Statistical comparison of the rostral and caudal extent of 4-HNE versus 3-NT immunostaining was carried out with paired t-tests at each time-point, with p < 0.05 required for significance.

Results

4-HNE and 3-NT immunostaining at the injury epicenter

This study showed that both 4-HNE and 3-NT accumulate rapidly after spinal cord injury, increasing as early as 3 h post-injury, with 3-NT remaining elevated until 1 week post-injury, and 4-HNE persisting until at least 2 weeks post-injury. The upper panels of Figure 1 show a comparison of representative coronal sections taken at the injury epicenter (T10) at 3, 24, and 72 h, and 1 and 2 weeks post-injury and stained for 4-HNE. The 4-HNE immunoreactivity was minimal in sham tissue harvested 24 h post-surgery. However, as early as 3 h post-injury, 4-HNE immunoreactivity was increased, with intense staining encompassing all of the gray matter and extending into the surrounding white matter. Staining peaked at 24 to 72 h post-injury, at which time there was only a small rim of white matter remaining unstained. The 4-HNE staining persisted out to 2 weeks post-injury, with a large portion of the gray matter still showing staining. The 3-NT immunoreactivity (lower panels of Fig. 1) showed a similar pattern to that of 4-HNE, with little to no reactivity in sham tissue. As with the 4-HNE, there was an increase in staining as early as 3 h post-injury, which peaked between 24 and 72 h post-injury. By 1 week post-injury, however, 3-NT staining had nearly disappeared, with only a small amount of gray matter showing staining, and by 2 weeks post-injury, immunoreactivity had returned to sham levels.

FIG. 1.
Representative examples showing the post-traumatic time course of oxidative damage as revealed by 3-NT and 4-HNE immunostaining. Images are representative coronal sections at the epicenter (T10) at 3, 24, and 72 h and at 1 and 2 weeks post-injury. ...

Rostral and caudal extent of 4-HNE and 3-NT immunostaining

The rostral-caudal extent of 4-HNE immunoreactivity is shown in Figure 2. Again, the sham animals show minimal background staining confined mostly to the gray matter. While staining was most intense at the injury epicenter (T10) at every time point, images taken every 2 mm show that immunoreactivity at 3, 24, and 72 h post-injury extends at least 6 mm in both the rostral and caudal directions. At 1 week post-injury, the epicenter still shows strong reactivity. However, staining in the rostral direction was reduced slightly when compared to the 72-h sections, and staining in the caudal direction had returned to sham levels. By 2 weeks post-injury, persistent reactivity was localized to the epicenter and sections immediately adjacent.

FIG. 2.
Rostral-caudal extent of 4-HNE staining at all time-points as shown by immunohistochemistry. All sections were counterstained with nuclear fast red. Images were taken every 2 mm starting 6 mm rostral to the epicenter and ending 6 mm ...

The 3-NT immunoreactivity showed a similar pattern to that of 4-HNE (Fig. 3). Reactivity in sham tissue was absent, and again the most intense staining at every time-point was found at the T10 epicenter. At 3 h post-injury, staining could be found in sections as much as 2–3 mm rostral to the epicenter and 6 mm caudal to the epicenter. By 24 h post-injury, staining in most animals extended 6 mm in either direction, a pattern that persisted to 72 h post-injury. In contrast to the 3-NT immunoreactivity, however, by 1 week post-injury staining had mostly disappeared and was confined to sections within 2 mm of the epicenter. Tissue harvested 2 weeks post-injury showed little to no reactivity in any sections.

FIG. 3.
Rostral-caudal extent of 3-NT staining at all time-points as shown by immunohistochemistry. All sections were counterstained with nuclear fast red. Images were taken every 2 mm starting 6 mm rostral to the epicenter, and ending 6 mm ...

The graphs in Figure 4 show a quantitative representation of the rostral-caudal distribution of both 4-HNE (top panel) and 3-NT (bottom panel) across the examined post-injury time-points (n = 4 for each). Sham animals showed no immunoreactivity and so were not included in the graphs. At 3 h post-injury, 4-HNE reactivity was found up to 6 mm in the rostral and caudal directions. At 24 h and 72 h post-injury, the rostral distribution was much the same (6 mm), but tissue as much as 9 mm in the caudal direction showed reactivity. By 1 week post-injury, staining was confined to sections 4 mm from the epicenter rostrally, and only 2 mm from the epicenter caudally. At 2 weeks post-injury, the area where reactivity occurred was further reduced, as staining was limited to an area within 1 mm of the epicenter in either direction. The mean rostral and caudal extents of 3-NT immunoreactivity were not significantly different than that of 4-HNE except at the initial (3-h) time-point when the rostral spread of 3-NT staining only extended 3 mm from the epicenter, compared to 6 mm for 4-HNE (p < 0.03). However, no significant difference was seen in the caudal distribution of 3-NT and 4-HNE at the 3-h time-point. Moreover, no differences in mean rostral or caudal extents of 3-NT versus 4-HNE were seen at any of the later time-points. At 24 h post-injury, 3-NT reactivity was found 4 mm rostral and 12 mm caudal to the epicenter. By 72 h post-injury the rostral-caudal extent of staining had decreased, and by 1 week post-injury, staining extended only 2 mm in either direction. At 2 weeks post-injury, only a small amount of staining was seen, localized to the injury epicenter.

FIG. 4.
Graphic summary of the rostral-caudal distribution of staining for both 4-HNE and 3-NT over various time-points in the injured rat spinal cord (values = mean ± SEM for 4 animals/time-point). The extent of staining ...

Microvascular and parenchymal 3-NT immunostaining

As shown in Figure 5, higher-power (20× and 40×) images from either 24 or 72 h post-injury were also examined for the purpose of identifying the relative 3-NT staining of microvascular versus parenchymal elements. The top row shows a 24-h post-injury spinal cord cross-section at 1 mm caudal to the epicenter. The 4× photo shows intense 3-NT staining of ventral horn motor neuron cell bodies. The 20× and 40× photos show three heavily stained microvessels (arrows). The middle row displays three magnifications of a 72-h post-injury cross-section 1 mm rostral to the epicenter, which shows an intensely 3-NT-stained microvessel (arrow). The 40× inset more clearly shows that the 3-NT staining in the microvessel wall extends out into the parenchyma, with gradually decreasing intensity as the distance from the vessel increases. This suggests that the microvessels may be a primary site of oxidative damage. The bottom row shows examples at 24 h that were taken 3 mm caudal to the epicenter, in which intense 3-NT staining of presumptive dorsal commissural axons and cell bodies is apparent. These examples illustrate that both microvessels and neuronal elements are targets of nitrative damage.

FIG. 5.
Higher-power views of 3-NT oxidative damage in the injured spinal cord. The top row shows a 24-h post-injury spinal cross-section at 1 mm caudal to the epicenter, and shows intense oxidative damage staining of gray matter microvessels (arrows), ...

Discussion

Previous studies have shown that there is an increase in 3-NT levels in spinal cord tissue during the first hours and days after acute contusion SCI (Bao et al., 2003; Liu et al., 2000; Scott et al., 1999; Scott et al., 2003; Xiong et al., 2007). Additional work has demonstrated that the infusion of PN at levels that mimic the estimated concentration produced in the contused spinal cord produces nitrative immunostaining and neuronal damage similar to that produced by contusion injury (Liu et al., 2005). The increase in 3-NT is paralleled by increases in LP products (Xiong et al., 2007) and the activation of the DNA repair enzyme poly-ADP ribose polymerase (Scott et al., 1999; Scott et al., 2004); both of these pathochemical events are known to be capable of being produced by PN (Beckman et al., 1990; Beckman, 1991; Beckman, 2002; Pacher et al., 2007; Radi et al., 1991). A recent report from our laboratory has shown that the coincident increase in the levels of 3-NT and the LP product 4-HNE during the first hour after injury persists at least until 7 days post-injury (Xiong et al., 2007). However, none of these prior reports has fully documented the spatial and temporal characteristics of PN-mediated oxidative damage in the injured cord related to protein nitration (3-NT) and LP (4-HNE).

Filling this knowledge gap, the current results show that contusion SCI increased 4-HNE and 3-NT substantially above sham levels (with minimal staining) as early as 3 h post-injury that peaked at 24 and 72 h post-injury. An increased level of staining persisted up to 1 week for 3-NT and for at least 2 weeks for 4-HNE. Oxidative damage staining was initially most intense in the gray matter and inner-most portions of white matter, but spread outwardly in white matter over time. The 4-HNE staining extended from 6 mm rostral to 9 mm caudal to the epicenter, with the largest extension found at 24 and 72 h post-injury. 3-NT showed a similar distribution, with the peak distribution occurring at 24 and 72 h and appearing as much as 6 mm rostral to the epicenter and 12 mm in the caudal direction. The overlap of 4-HNE and 3-NT staining temporally as well as spatially suggests that PN contributes to LP as well as protein nitration after SCI. Importantly, the 3-NT and 4-HNE immunohistochemistry demonstrates that PN-mediated oxidative damage simultaneously affects spinal cord microvessels and neuronal soma, dendrites, and axons. This broad spectrum of PN cellular damage has also been seen in the injured brain (Deng et al., 2007; Hall et al., 2004). This suggests that therapeutic attempts to inhibit oxidative damage by PN need to target both the microvasculature and the neural parenchyma.

Recent experiments have strongly indicated that a key cellular source of post-traumatic PN in either spinal cord or brain injury are mitochondria. Mitochondria have been demonstrated to contain a constitutive NOS isoform that is activated by traumatically-induced intracellular calcium, and that the resulting increase in •NO together with an increase in mitochondrial leakage of O2•− leads to PN generation (Deng et al., 2007; Hall et al., 2004; Radi et al., 2002b; Xiong et al., 2007). Consistent with this scenario, SCI and TBI initiate mitochondrial respiratory and calcium-buffering dysfunction during the first few post-traumatic hours, together with an increase in 3-NT and 4-HNE modification of mitochondrial proteins (Deng et al., 2007; Singh et al., 2006; Xiong et al., 2007). Similarly, exposure of normal (non-injured) CNS mitochondria to PN replicates post-traumatic mitochondrial respiratory dysfunction and 3-NT modification of mitochondrial proteins (Singh et al., 2007). Thus, mitochondria appear to be both a source of post-traumatic PN generation, and are likely an initial site of PN-mediated oxidative damage. This leads to loss of normal oxidative phosphorylation (i.e., ATP synthesis) and calcium-buffering capacity that becomes apparent as early as 6 h and peaks at 24 h post-injury (Sullivan et al., 2007). However, while the mitochondrial generation of PN may be important during the first 24 h after SCI, there is considerable evidence that the generation of PN is also contributed to by delayed cellular inflammatory mechanisms involving activation of inducible nitric oxide synthase (iNOS). For example, iNOS enzymatic activity has been shown to be increased in the contused rat spinal cord as early as 24 h post-injury, coincident with the invasion of polymorphonuclear leukocytes (PMNs), and administration of the selective iNOS inhibitor aminoguanidine can improve neurological and histological outcomes (Chatzipanteli et al., 2002). A second group has demonstrated upregulation of iNOS expression as early as 2 h after spinal cord contusion injury that peaks at 72 h. A third set of investigators has reported that the increase in 3-NT until 7 days occurs together with an increase in the levels of iNOS in activated microglia in the injured cord (Xu et al., 2001). Thus it would appear that over much of the acute (first 24 h) and subacute (24 h to 1 week) SCI time course, the generation of •NO, and ultimately PN, comes at least from a combination of mitochondrial constitutive NOS activation and inflammatory cell iNOS upregulation (Conti et al., 2007). Furthermore, keep in mind that some of the •NO2 that mediates tyrosine nitration can be generated from the heme-containing myeloperoxidase (MPO) in either infiltrating PMNs or macrophages, as well as in activated spinal cord microglia. The chemistry of this enzymatic source involves MPO-catalyzed conversion of nitrite anion (NO2) to •NO2 (Radi, 2004). This non-PN source of 3-NT is more likely to contribute at 24 h or later post-injury, the times when the most inflammatory cell infiltration occurs (Donnelly and Popovich, 2008).

The current results, as well as those of earlier studies (Bao et al., 2003; Genovese et al., 2007; Liu et al., 2005; Scott et al., 1999; Scott et al., 2003; Scott et al., 2005; Xiong et al., 2007; Xiong and Hall, 2009), suggest that the administration of antioxidants that target PN or PN-derived radicals within the first hours after SCI should provide effective neuroprotection. In agreement with this idea, compounds that can either scavenge peroxynitrite such as uric acid (Scott et al., 2005) or FeTSPP (Genovese et al., 2007), or its derived free radicals such as tempol (Hillard et al., 2004; Xiong and Hall, 2009) have been reported to attenuate post-SCI spinal tissue damage. The neuroprotective effects of the latter compound, tempol, have been recently shown to occur together with a decrease in mitochondrial dysfunction, protein oxidative damage, and calcium-mediated cytoskeletal proteolytic degradation (Xiong et al., 2007; Xiong and Hall, 2009). In any event, the current results strongly suggest that future therapeutic attempts to inhibit post-traumatic oxidative damage in SCI need to (1) be directed at PN or its derived free radicals; (2) be initiated as early as possible, ideally within the first few hours; (3) protect both microvascular and neuronal cells; and (4) may need to be continued for as long as 7–14 days post-injury.

Acknowledgments

This work was supported by a grant from the Kentucky Spinal Cord & Head Injury Research Trust (5P30 NS051220).

Author Disclosure Statement

No conflicting financial interest exist.

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