• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Oct 1992; 66(10): 6223–6225.
PMCID: PMC283677

Bovine leukemia virus gene expression in vivo.


The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (761K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
  • Coulston J, Daniel RC, Lavin MF. Integration of bovine leukaemia virus at all stages of enzootic bovine leukosis. Arch Virol. 1991;119(1-2):13–23. [PubMed]
  • Cullen BR. Human immunodeficiency virus as a prototypic complex retrovirus. J Virol. 1991 Mar;65(3):1053–1056. [PMC free article] [PubMed]
  • Gessain A, Louie A, Gout O, Gallo RC, Franchini G. Human T-cell leukemia-lymphoma virus type I (HTLV-I) expression in fresh peripheral blood mononuclear cells from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy. J Virol. 1991 Mar;65(3):1628–1633. [PMC free article] [PubMed]
  • Heeney JL, Valli VE. Transformed phenotype of enzootic bovine lymphoma reflects differentiation-linked leukemogenesis. Lab Invest. 1990 Mar;62(3):339–346. [PubMed]
  • Hidaka M, Inoue J, Yoshida M, Seiki M. Post-transcriptional regulator (rex) of HTLV-1 initiates expression of viral structural proteins but suppresses expression of regulatory proteins. EMBO J. 1988 Feb;7(2):519–523. [PMC free article] [PubMed]
  • Jensen WA, Rovnak J, Cockerell GL. In vivo transcription of the bovine leukemia virus tax/rex region in normal and neoplastic lymphocytes of cattle and sheep. J Virol. 1991 May;65(5):2484–2490. [PMC free article] [PubMed]
  • Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T. Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5620–5624. [PMC free article] [PubMed]
  • Orita S, Saiga A, Takagi S, Tanaka T, Okumura K, Aono Y, Hinuma Y, Igarashi H. A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein. FEBS Lett. 1991 Dec 16;295(1-3):127–134. [PubMed]
  • Pomerantz RJ, Trono D, Feinberg MB, Baltimore D. Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: a molecular model for latency. Cell. 1990 Jun 29;61(7):1271–1276. [PubMed]
  • Powers MA, Grossman D, Kidd LC, Radke K. Episodic occurrence of antibodies against the bovine leukemia virus Rex protein during the course of infection in sheep. J Virol. 1991 Sep;65(9):4959–4965. [PMC free article] [PubMed]
  • Schmitz GG, Walter T, Seibl R, Kessler C. Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal Biochem. 1991 Jan;192(1):222–231. [PubMed]
  • Smith MR, Greene WC. Molecular biology of the type I human T-cell leukemia virus (HTLV-I) and adult T-cell leukemia. J Clin Invest. 1991 Mar;87(3):761–766. [PMC free article] [PubMed]
  • Willems L, Heremans H, Chen G, Portetelle D, Billiau A, Burny A, Kettmann R. Cooperation between bovine leukaemia virus transactivator protein and Ha-ras oncogene product in cellular transformation. EMBO J. 1990 May;9(5):1577–1581. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...