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J Virol. Oct 1992; 66(10): 6223–6225.
PMCID: PMC283677

Bovine leukemia virus gene expression in vivo.

Abstract

The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops.

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  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
  • Coulston J, Daniel RC, Lavin MF. Integration of bovine leukaemia virus at all stages of enzootic bovine leukosis. Arch Virol. 1991;119(1-2):13–23. [PubMed]
  • Cullen BR. Human immunodeficiency virus as a prototypic complex retrovirus. J Virol. 1991 Mar;65(3):1053–1056. [PMC free article] [PubMed]
  • Gessain A, Louie A, Gout O, Gallo RC, Franchini G. Human T-cell leukemia-lymphoma virus type I (HTLV-I) expression in fresh peripheral blood mononuclear cells from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy. J Virol. 1991 Mar;65(3):1628–1633. [PMC free article] [PubMed]
  • Heeney JL, Valli VE. Transformed phenotype of enzootic bovine lymphoma reflects differentiation-linked leukemogenesis. Lab Invest. 1990 Mar;62(3):339–346. [PubMed]
  • Hidaka M, Inoue J, Yoshida M, Seiki M. Post-transcriptional regulator (rex) of HTLV-1 initiates expression of viral structural proteins but suppresses expression of regulatory proteins. EMBO J. 1988 Feb;7(2):519–523. [PMC free article] [PubMed]
  • Jensen WA, Rovnak J, Cockerell GL. In vivo transcription of the bovine leukemia virus tax/rex region in normal and neoplastic lymphocytes of cattle and sheep. J Virol. 1991 May;65(5):2484–2490. [PMC free article] [PubMed]
  • Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T. Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5620–5624. [PMC free article] [PubMed]
  • Orita S, Saiga A, Takagi S, Tanaka T, Okumura K, Aono Y, Hinuma Y, Igarashi H. A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein. FEBS Lett. 1991 Dec 16;295(1-3):127–134. [PubMed]
  • Pomerantz RJ, Trono D, Feinberg MB, Baltimore D. Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: a molecular model for latency. Cell. 1990 Jun 29;61(7):1271–1276. [PubMed]
  • Powers MA, Grossman D, Kidd LC, Radke K. Episodic occurrence of antibodies against the bovine leukemia virus Rex protein during the course of infection in sheep. J Virol. 1991 Sep;65(9):4959–4965. [PMC free article] [PubMed]
  • Schmitz GG, Walter T, Seibl R, Kessler C. Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal Biochem. 1991 Jan;192(1):222–231. [PubMed]
  • Smith MR, Greene WC. Molecular biology of the type I human T-cell leukemia virus (HTLV-I) and adult T-cell leukemia. J Clin Invest. 1991 Mar;87(3):761–766. [PMC free article] [PubMed]
  • Willems L, Heremans H, Chen G, Portetelle D, Billiau A, Burny A, Kettmann R. Cooperation between bovine leukaemia virus transactivator protein and Ha-ras oncogene product in cellular transformation. EMBO J. 1990 May;9(5):1577–1581. [PMC free article] [PubMed]

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