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Figure 1

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Expression of fumarase from the mitochondrial genome.

(A) Mitochondrial encoded fumarase is enzymatically active. WT, Δfum1, and Δfum1/Fum1m (Fum1m) strains were serially diluted (10−1, 10−2, 10−3, 10−4) and grown on fermentable, glucose or non-fermentable, glycerol medium. (B) Mitochondrial encoded fumarase is exclusively localized to mitochondria. WT and Fum1m strains were subjected to subcellular fractionation as previously described [7]. Equivalent portions of the total (Tot), cytosolic (Cyt), and mitochondrial (Mit) fractions were analyzed by Western blotting using the indicated antibodies. Hsp60 and HxK1 were used as markers for the mitochondria and the cytosol, respectively.

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