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Proc Natl Acad Sci U S A. Mar 1970; 65(3): 737–744.
PMCID: PMC282968

Selection and Characterization of an E. coli Mutant Defective in Membrane Lipid Biosynthesis*

Abstract

A procedure is described for the selection of temperature-sensitive phospholipid mutants based upon radiation suicide of the wild-type organisms by tritiated L-glycerol-3-phosphate incorporated in the phospholipids.

One of these mutants possesses an acyltransferase activity much more thermolabile than that of its parent. This mutant ceases growth and phospholipid biosynthesis immediately upon shift to a nonpermissive temperature although DNA, RNA, and protein continue to be synthesized. The phenotype of this mutant appears due to a single mutation by reversion analysis and by enzymatic analysis of temperature-resistant revertants.

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Selected References

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  • Rothfield L, Finkelstein A. Membrane biochemistry. Annu Rev Biochem. 1968;37:463–496. [PubMed]
  • Silbert DF, Vagelos PR. Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579–1586. [PMC free article] [PubMed]
  • Silbert DF, Ruch F, Vagelos PR. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J Bacteriol. 1968 May;95(5):1658–1665. [PMC free article] [PubMed]
  • HAYASHI S, KOCH JP, LIN EC. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed]
  • Cronan JE, Jr, Birge CH, Vagelos PR. Evidence for two genes specifically involved in unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1969 Nov;100(2):601–604. [PMC free article] [PubMed]
  • VOGEL HJ, BONNER DM. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed]
  • Carter JR., Jr Cytidine triphosphate: phosphatidic acid cytidyltransferase in Escherichia coli. J Lipid Res. 1968 Nov;9(6):748–754. [PubMed]
  • Hutchison HT, Cronan JE., Jr The synthesis of cytidine diphosphate diglyceride by cell-free extracts of yeast. Biochim Biophys Acta. 1968 Dec 18;164(3):606–608. [PubMed]
  • KANFER J, KENNEDY EP. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. II. BIOSYNTHESIS OF PHOSPHOLIPIDS IN ESCHERICHIA COLI. J Biol Chem. 1964 Jun;239:1720–1726. [PubMed]
  • MUNKRES KD, RICHARDS FM. THE PURIFICATION AND PROPERTIES OF NEUROSPORA MALATE DEHYDROGENASE. Arch Biochem Biophys. 1965 Mar;109:466–479. [PubMed]
  • Cronan JE, Jr, Wulff DL. A role for phospholipid hydrolysis in the lysis of Escherichia coli infected with bacteriophage T4. Virology. 1969 Jun;38(2):241–246. [PubMed]
  • Cronan JE., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. [PMC free article] [PubMed]
  • Chang YY, Kennedy EP. Biosynthesis of phosphatidyl glycerophosphate in Escherichia coli. J Lipid Res. 1967 Sep;8(5):447–455. [PubMed]
  • Furrow MH, Pizer LI. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages. J Virol. 1968 Jun;2(6):594–605. [PMC free article] [PubMed]
  • Ames GF. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. [PMC free article] [PubMed]
  • Ailhaud GP, Vagelos PR. Palmityl-acyl carrier protein as acyl donor for complex lipid biosynthesis in Escherichia coli. J Biol Chem. 1966 Aug 25;241(16):3866–3869. [PubMed]
  • Kito M, Pizer LI. Phosphatidic acid synthesis in Escherichia coli. J Bacteriol. 1969 Mar;97(3):1321–1327. [PMC free article] [PubMed]
  • Chang YY, Kennedy EP. Pathways for the synthesis of glycerophosphatides in Escherichia coli. J Biol Chem. 1967 Feb 10;242(3):516–519. [PubMed]
  • Luria SE, Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. [PMC free article] [PubMed]
  • Böck A. Relation between subunit structure and temperature-sensitivity of mutant phenylalanyl RNA synthetases of Escherichia coli. Eur J Biochem. 1968 Apr;4(3):395–400. [PubMed]
  • Cozzarelli NR, Koch JP, Hayashi S, Lin EC. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1325–1329. [PMC free article] [PubMed]
  • Kito M, Lubin M, Pizer LI. A mutant of Escherichia coli defective in phosphatidic acid synthesis. Biochem Biophys Res Commun. 1969 Feb 21;34(4):454–458. [PubMed]

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