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Proc Natl Acad Sci U S A. 1970 Feb; 65(2): 357–362.
PMCID: PMC282910

A Specific Complex between a Restriction Endonuclease and Its DNA Substrate*


In the presence of Mg++, ATP, and S-adenosylmethionine, the DNA restriction endonuclease R·K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.

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Selected References

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  • Meselson M, Yuan R. DNA restriction enzyme from E. coli. Nature. 1968 Mar 23;217(5134):1110–1114. [PubMed]
  • Linn S, Arber W. Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1300–1306. [PMC free article] [PubMed]
  • Roulland-Dussoix D, Boyer HW. The Escherichia coli B restriction endonuclease. Biochim Biophys Acta. 1969 Nov 19;195(1):219–229. [PubMed]
  • Arber W, Linn S. DNA modification and restriction. Annu Rev Biochem. 1969;38:467–500. [PubMed]
  • Jones OW, Berg P. Studies on the binding of RNA polymerase to polynucleotides. J Mol Biol. 1966 Dec 28;22(2):199–209. [PubMed]
  • Yarus M, Berg P. Recognition of tRNA by aminoacyl tRNA synthetases. J Mol Biol. 1967 Sep 28;28(3):479–490. [PubMed]
  • Riggs AD, Bourgeois S, Newby RF, Cohn M. DNA binding of the lac repressor. J Mol Biol. 1968 Jul 14;34(2):365–368. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


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