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Curr Opin Microbiol. Author manuscript; available in PMC Feb 1, 2011.
Published in final edited form as:
PMCID: PMC2822058
NIHMSID: NIHMS167840

The bacterial essence of tiny symbiont genomes

Abstract

Bacterial genomes vary in size over two orders of magnitude. The Mycoplasma genitalium genome has traditionally defined the extreme small end of this spectrum, and has therefore heavily informed theoretical and experimental work aimed at determining the minimal gene content necessary to support cellular life. Recent genomic data from insect symbionts has revealed bacterial genomes that are incredibly small—two to four times smaller than M. genitalium—and these tiny genomes have raised questions about the limits of genome reduction and have blurred the once-clear distinction between autonomous cellular life and highly integrated organelle. New data from various systems with symbiotic bacterial or archaeal partners have begun to shed light on how these bacteria may function with such small gene sets, but major mechanistic questions remain.

Introduction

In most bacterial genomes, genes are tightly packed and uniformly distributed at about one gene per kilobase (kb) [1], so that in most cases genome reduction implies gene loss. Bacteria that have close associations with animals often show reduced genomes compared to free-living relatives [24], and for decades the smallest cellular genome observed in nature was from the human pathogen M. genitalium [5,6]. As the ancestors of both mitochondria and chloroplasts were free-living bacteria [7,8], they can be considered the most extreme examples of bacterial genome reduction. Despite their bacterial origins, however, mitochondria and chloroplasts are defined as cellular organelles, not as autonomous bacteria. This distinction is based on lifestyle and gene content: M. genitalium can be grown in the lab, while organelles are highly genetically integrated with the nucleus and are completely dependent on being in the host environment [7,8]; M. genitalium has 524 genes in a 580 kb genome [6], while the largest mitochondrial genome has 97 genes in a 69 kb genome [9], and the most gene-rich chloroplast genome has 253 genes in a 191 kb genome [10]. A long-standing empirical limit for genome reduction in autonomous bacteria was therefore established by the mycoplasma, however they remain clearly distinct from organelles by almost any measure except their shared bacterial ancestry.

This clean differentiation between organelle and independent bacteria has been muddied in the last few years by data from genome sequencing projects targeting uncultured intracellular symbionts of insects. This review will briefly describe these tiny symbiont genomes and discuss them in the context of the minimal genome concept, compare their gene content with that of organelles, and summarize recent experiments that give the first clues as to how these organisms might survive with such small gene sets.

Bacterial endosymbionts of insects

Like all animals, insects form associations with diverse bacterial lineages [4]. These symbioses vary by type, falling anywhere on the parasitic-commensal-mutualistic continuum. Once established, these relationships are not necessarily static, sometimes rapidly switching between association type (e.g., from parasite to mutualist [11**]). The intimacy of the interactions can also vary, as symbionts can be horizontally transferred among unrelated insects and/or strictly vertically transmitted in a species-specific manner, and are found in a wide range of tissues, from the extracellular space of the gut to the cytoplasm of specialized host cells. A well-known example of an intracellular parasite that can be either horizontally or vertically transferred is the reproductive manipulator Wolbachia, an α-Proteobacteria which skews the sex ratios of offspring in infected mothers [12*]. Many insects with restricted or specialized diets (e.g., plant sap, animal blood) have one or more intracellular bacterial mutualist, which provision the insect with nutrients that are missing in their diet [13,14]. These associations are usually extremely stable—in some cases cospeciating for hundreds of millions of years—by virtue of strict transovarial transmission of the symbionts through insect generations [15,16]. Most of these associations are thought to be reciprocally obligate, i.e., neither the insect nor its symbiotic bacteria can survive without the other [14,17]. These symbionts also tend to have highly reduced genomes compared to their free-living relatives [4].

The first several insect nutritional symbionts to have their genomes sequenced—all γ-Proteobacteria—included three strains of the aphid symbiont Buchnera aphidicola [15,18,19], the tsetse fly symbiont Wigglesworthia glossinidia [20], and two strains of the carpenter ant symbiont Blochmannia [21,22]. While all of these symbionts showed significant levels of genome reduction (616 – 792 kb) and their limited gene sets indicated they could not (easily) live outside of the host cell environment, their genome sizes were above the minimal size threshold established by M. genitalium (although physical mapping of various Buchnera strains indicated that some had smaller genomes, in the range of 450 kb [23]).

Recent results from genome sequencing of diverse bacterial symbionts of sap-feeding insects have begun to blur the clear distinction between independent bacterial life and organelle, crashing through the 500 kb genome barrier established by M. genitalium in dramatic fashion. In 2006, the 422 kb genome from Buchnera aphidicola Cc [24] and the 160 kb genome from Carsonella ruddii [25], a γ-Proteobacterial symbiont of psyllid, were reported. The next year, a Bacteroidetes called Sulcia muelleri, which is symbiotic with the glassy-winged sharpshooter, was reported to have a genome of 245 kb [26]. Finally, in 2009, the genome for an α-Proteobacterial symbiont of singing cicadas, Hodgkinia cicadicola, was shown to have a genome of only 144 kb, encoding a paltry 188 genes [27*]. (Carsonella is the sole symbiont in the species of psyllid studied, but Buchnera Cc [28], Sulcia [16,26,29], and Hodgkinia [30] all have co-symbionts inhabiting the same insect tissue; Sulcia and Hodgkinia are partners in cicada.) Amazingly, Carsonella and Hodgkinia have smaller genomes and fewer protein-coding genes than some chloroplasts (Fig. 1), and questions as to whether or not these organisms can still be considered autonomous bacteria have arisen [31*,32].

Figure 1
Gene content of the smallest cellular genomes and some organelles

Metabolic vs. genetic integration and the minimal genome concept

The small genome of Mycoplasma genitalium has made it a central player in the “minimal genome concept,” which can be defined as the experimental and computational search for the minimal gene content required for independent life, given the richest possible growth environment [3339]. Predictions of the minimal genome, based on either comparative genomics [33,37] or global transposition mutagenesis of M. genitalium [38], range from about 200 to 400 genes.

The organism(s) that would fulfill the minimal genome concept are usually, but not always [39], assumed to be both genetically and metabolically independent. That is, these organisms would be capable of replicating their genome, transcribing RNA, and translating protein (genetic independence); and would be able to obtain energy from simple metabolites to make nucleotides, amino acids, lipids, and cofactors (metabolic independence). Gene content analysis of Buchnera Cc, Sulcia, Carsonella, and Hodgkinia reveal that these organisms are not metabolically independent, as they cannot make fatty acids (except Buchnera), phospholipids, nucleotides, pyridines, and in the case of Buchnera Cc and Hodgkinia, have lost their F1F0 ATP synthase. This loss of metabolic independence is typical of both intracellular [2] and extracellular [40] symbionts. It is assumed that the required compounds are somehow derived from the host (or possibly a co-symbiont, in some systems), but the mechanisms are not well understood. Therefore, the remainder of this discussion will focus on the potential genetic independence of the most highly reduced symbiont genomes.

The gene contents of symbionts and organelles are different

While the number of genes predicted in the smallest symbiont genomes rival that of some organelles, gene content analysis reveals a clear difference in retained activities (Fig. 1). Insect symbionts have retained genes involved in the core enzymatic activities involved in chromosome replication, transcription, and translation, while in organellar genomes many of these functions have been lost, with some exceptions (Fig. 1). For example, all of the bacterial symbionts contain a homolog of the core replicative DNA polymerase (dnaE), the protein responsible for the 5′ to 3′ polymerization activity of the replication holoenzyme, but lack homologs for many of the accessory components involved in increasing processivity, initiation, and error correction (Fig. 1). These patterns suggest, not surprisingly [8], that the forces governing gene loss in symbionts and organelles are different. Although it is not at all clear how the genes present in symbiont genomes could work to form a fully functional replicating unit, they do suggest a stronger bacterial identity for nutritional symbionts than for organelles.

There are a number of possible ways these symbionts could cope with such small gene sets, such as: i) the transfer some genes to the nucleus for subsequent reimportation, similar to what is observed in organelles; ii) the importation host (or co-symbiont) proteins or RNAs that complement the lost activities; or, perhaps most interestingly, iii) the evolution of unexpected coadaptations to the loss of various genes, resulting in mechanisms for cellular processes that are difficult to predict. While some data exist concerning the host’s role in the symbiosis [41], there is no information presently available concerning the import of proteins or RNA into these symbionts, so this point will not be discussed further.

Is gene transfer the answer?

Given the extremely small gene sets of these insect endosymbionts, it is tempting to speculate whether some of the lost genes have been transferred to the host nucleus for subsequent expression and protein reimportation to the symbiont [25,42], as this process has occurred with some frequency in organelles, and in fact has been shown to be ongoing in some cases [8]. This idea might be considered particularly seductive given the apparent ease with which Wolbachia species—another transovarially transmitted intracellular bacterial symbiont found in insects and other invertebrates—have been shown to exchange DNA with the host nucleus [4349]. Remarkably, some of these Wolbachia-to-host transfers include DNA fragments approaching the size of entire Wolbachia genomes (about 1 Mb) [44**]. Early evidence suggested that the majority of these transferred genes were non-functional, as they typically are not expressed at high levels and contain mutations that would result in nonfunctional proteins if expressed in the recipient host cell [44**,49,50]. However, recent experiments from various systems have shown that some transferred genes might be functional, in that they contain no premature stop codons, are undergoing purifying selection, and in some cases are expressed at high levels in the appropriate tissues [4547].

Of particular relevance here is the report of transferred bacterial genes in the pea aphid Acyrthosiphon pisum [47], as the pea aphid is host to Buchnera aphidicola, a long-term coevolving bacterial symbiont with a reduced genome. While Buchnera from the pea aphid does not show as much genome reduction as Hodgkinia, Carsonella, or Sulcia, at 641 kb it is still a small bacterial genome [18], and its publication has fueled speculation that some lost genes might have been transferred to the host nucleus [42]. By analyzing an mRNA expression library made from aphid tissues for genes that looked bacterial in nature, two potential transfers were identified: ldcA (LD-carboxypeptidase) and rplA (rare lipoprotein A) [47]. Phylogenetic analysis indicated that ldcA was derived from a Wolbachia-like α-Proteobacteria, while the classification of rplA was less clear [47]. Importantly, both genes were preferentially expressed in the tissue type containing bacterial symbionts [47]. These results suggest two interesting possibilities: i) the maintenance of some symbioses may be aided by genes transferred to the host from unrelated bacterial lineages, and ii) lost Buchnera genes could be complemented by genes transferred to the host nucleus from an unrelated symbiotic bacterium such as Wolbachia. Although these data are preliminary, they also hint at the possibility that the large amount of genome reduction seen in insect symbionts may not have been accompanied by gene transfer to the host nucleus, as no clear case of gene transfer from Buchnera was observed in this study. It should be noted that firm results on the number of potentially transferred Buchnera genes will soon be available upon completion and analysis of the pea aphid genome [NCBI Aphid Genome Resources; URL: www.ncbi.nlm.nih.gov/projects/genome/guide/aphid/].

It is important to note that although both Wolbachia and insect nutritional symbionts are transferred via a transovarial route, the timing and cell biology of these transfers are different. In the fruit fly, Wolbachia is intimately associated with germ line cells throughout the development of an infected insect, including cytoplasmic localization in the germ line stem cells and physical association with oocyte nuclei at later points in oogenesis (e.g., see [51]). By contrast, in aphid development (the best studied system for insect nutritional symbionts, though the rough outlines seem similar in other sap-feeding insects [13]), Buchnera cells are not transferred to the oocyte until later in oogenesis, where the bacteria are held in a matrix of filamentous actin at the posterior end of the egg until being cellularized by the developing embryo (e.g., see [52]). If further work continues to show a dearth of gene transfer between nutritional symbionts and their hosts compared with Wolbachia, the close association with the germ line in the latter may account for the difference.

Unexpected coadaptations to gene loss

The concept of an “essential” gene is difficult to precisely define. Some genes are required only in certain metabolic contexts, and other genes found to be required experimentally in one bacterial lineage are completely missing in other lineages [36,53,54]. Furthermore, there are only about 60 universally conserved proteins derived from the analysis of genome projects, this list being dominated by translation related functions [36]. Clearly, though, there are a core of highly conserved genes that seem to have essential activities for which it is difficult to imagine how the cell survives without. One possible solution to the problem of “essential” gene loss that is rarely mentioned is the emergence of novel coadaptations elsewhere in the genome to accommodate the lost activity [54]. The main problem with this solution is that mechanisms are difficult to imagine in many cases, and concrete examples have been rare until recently.

The most compelling example of coadaptation to the loss of an “essential” gene comes from the smallest Archaeal genome, Nanoarchaeum equitans, the extracellular symbiont of Ignicoccus hospitalis (itself an archaeon) [40]. Nanoarchaeum—as well as Sulcia, Carsonella, and Hodgkinia—lacks the ribonucleoprotein RNase P, the enzyme involved in processing 5′ leader sequence from tRNAs. RNase P is a (nearly) ubiquitous enzyme, and therefore is included in even the smallest proposed minimal genome [37]. The absence of RNase P in Nanoarchaeum prompted Söll and colleagues to look at this system more closely, where they found that unlike most organisms, Nanoarchaeum tRNAs have transcriptional promoters placed at uniform distances upstream of the first base of the tRNA [55**]. This precise promoter positioning allows for leaderless tRNAs; if transcription always starts at the first base of tRNA, RNase P is no longer needed. This result shows how the cell can cope with the loss of an “essential” and nearly universal gene in a novel and unexpected way, and serves as a warning not to expect cellular processes, even highly conserved and seemingly essential ones, to proceed by standard mechanisms in highly reduced symbiont genomes.

Conclusions

Continued sequencing of symbiont genomes, whether from insects or elsewhere, will likely continue to uncover organisms with even smaller gene sets than the ones discussed here. These genomes will continue to contribute to our understanding of the breadth and depth of bacterial symbioses with animals, but will likely not advance the field in terms of understanding how these organisms survive with such limited gene sets. It seems reasonable that the answer lies in a complex combination of metabolite, protein, and/or RNA importation combined with both small incremental and large unexpected coadaptations to the loss of genes. Untangling this web will not be easy, as none of these insect systems containing the smallest symbiont genomes are currently genetically tractable or even easily cultured in the lab. Progress will have to come from creative biochemical and cell biological experiments that complement the intriguing genomic data described here.

Acknowledgments

J.P.M. was funded by the Center for Insect Science at the University of Arizona through National Institutes of Health Training Grant # 1K12 GM000708.

Footnotes

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