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Proc Natl Acad Sci U S A. Sep 1988; 85(18): 6622–6626.
PMCID: PMC282029

A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.

Abstract

A highly conserved repetitive DNA sequence, (TTAGGG)n, has been isolated from a human recombinant repetitive DNA library. Quantitative hybridization to chromosomes sorted by flow cytometry indicates that comparable amounts of this sequence are present on each human chromosome. Both fluorescent in situ hybridization and BAL-31 nuclease digestion experiments reveal major clusters of this sequence at the telomeres of all human chromosomes. The evolutionary conservation of this DNA sequence, its terminal chromosomal location in a variety of higher eukaryotes (regardless of chromosome number or chromosome length), and its similarity to functional telomeres isolated from lower eukaryotes suggest that this sequence is a functional human telomere.

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  • Singer MF. Highly repeated sequences in mammalian genomes. Int Rev Cytol. 1982;76:67–112. [PubMed]
  • Weiner AM, Deininger PL, Efstratiadis A. Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem. 1986;55:631–661. [PubMed]
  • Rich A, Nordheim A, Wang AH. The chemistry and biology of left-handed Z-DNA. Annu Rev Biochem. 1984;53:791–846. [PubMed]
  • Blackburn EH, Challoner PB. Identification of a telomeric DNA sequence in Trypanosoma brucei. Cell. 1984 Feb;36(2):447–457. [PubMed]
  • Van der Ploeg LH, Liu AY, Borst P. Structure of the growing telomeres of Trypanosomes. Cell. 1984 Feb;36(2):459–468. [PubMed]
  • Blackburn EH. The molecular structure of centromeres and telomeres. Annu Rev Biochem. 1984;53:163–194. [PubMed]
  • Yudkin J. Sugar and disease. Nature. 1972 Sep 22;239(5369):197–199. [PubMed]
  • Greider CW, Blackburn EH. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell. 1987 Dec 24;51(6):887–898. [PubMed]
  • Moyzis RK, Albright KL, Bartholdi MF, Cram LS, Deaven LL, Hildebrand CE, Joste NE, Longmire JL, Meyne J, Schwarzacher-Robinson T. Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma. 1987;95(6):375–386. [PubMed]
  • Robertson AD, Moyzis RK, Bonnet J, Ts'o PO. A probabilistic analysis of DNA sequence organization. J Mol Biol. 1981 Dec 25;153(4):864–870. [PubMed]
  • Moyzis RK, Bonnet J, Li DW, Ts'o PO. An alternative view of mammalian DNA sequence organization. II. Short repetitive sequences are organized into scrambled tandem clusters in Syrian hamster DNA. J Mol Biol. 1981 Dec 25;153(4):871–896. [PubMed]
  • Deng G, Wu R. An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA. Nucleic Acids Res. 1981 Aug 25;9(16):4173–4188. [PMC free article] [PubMed]
  • Clarke L, Carbon J. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976 Sep;9(1):91–99. [PubMed]
  • Schmid CW, Deininger PL. Sequence organization of the human genome. Cell. 1975 Nov;6(3):345–358. [PubMed]
  • Meinkoth J, Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. [PubMed]
  • Deaven LL, Van Dilla MA, Bartholdi MF, Carrano AV, Cram LS, Fuscoe JC, Gray JW, Hildebrand CE, Moyzis RK, Perlman J. Construction of human chromosome-specific DNA libraries from flow-sorted chromosomes. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):159–167. [PubMed]
  • Forney J, Henderson ER, Blackburn EH. Identification of the telomeric sequence of the acellular slime molds Didymium iridis and Physarum polycephalum. Nucleic Acids Res. 1987 Nov 25;15(22):9143–9152. [PMC free article] [PubMed]
  • Southern EM. Base sequence and evolution of guinea-pig alpha-satellite DNA. Nature. 1970 Aug 22;227(5260):794–798. [PubMed]
  • Fry K, Salser W. Nucleotide sequences of HS-alpha satellite DNA from kangaroo rat Dipodomys ordii and characterization of similar sequences in other rodents. Cell. 1977 Dec;12(4):1069–1084. [PubMed]
  • Holmquist GP, Dancis B. Telomere replication, kinetochore organizers, and satellite DNA evolution. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4566–4570. [PMC free article] [PubMed]
  • Henderson E, Hardin CC, Walk SK, Tinoco I, Jr, Blackburn EH. Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs. Cell. 1987 Dec 24;51(6):899–908. [PubMed]
  • Oka Y, Thomas CA., Jr The cohering telomeres of Oxytricha. Nucleic Acids Res. 1987 Nov 11;15(21):8877–8898. [PMC free article] [PubMed]
  • Blackburn EH, Gall JG. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978 Mar 25;120(1):33–53. [PubMed]
  • Yao MC, Yao CH. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436–7439. [PMC free article] [PubMed]
  • Burke DT, Carle GF, Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. [PubMed]
  • McKusick VA, Ruddle FH. Toward a complete map of the human genome. Genomics. 1987 Oct;1(2):103–106. [PubMed]
  • Schwartz DC, Cantor CR. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. [PubMed]
  • Carle GF, Olson MV. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. [PMC free article] [PubMed]
  • Smith HO, Birnstiel ML. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. [PMC free article] [PubMed]
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed]
  • Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science. 1987 Mar 27;235(4796):1616–1622. [PubMed]

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