• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Sep 1988; 85(17): 6419–6423.
PMCID: PMC281983

Conservation of gene repertoire but not gene order in pepper and tomato


Homologies of tomato and pepper genes have been compared, and genetic linkage maps have been constructed based on a common set of cDNA clones and selected single-copy genomic clones. We report here that the gene repertoire of these two species is highly conserved, yet the linear order of the genes on the chromosomes has been greatly modified. Although the two species share the same number of centromeres, the chromosomal regions around those centromeres have undergone extensive rearrangements. Accompanying the extensive chromosome rearrangement has been a change in locus number for approximately 12% of the loci detected by random cDNA clones. Duplicated loci within each genome are normally found on different chromosomes and are not confined to one species, thus ruling out gene duplication as an explanation for the 4-fold higher DNA content of pepper. At least one of the duplications occurred since the divergence of tomato and pepper from their last common ancestor.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science. 1983 Jun 3;220(4601):1049–1051. [PubMed]
  • O'Brien SJ, Nash WG. Genetic mapping in mammals: chromosome map of domestic cat. Science. 1982 Apr 16;216(4543):257–265. [PubMed]
  • Womack JE, Moll YD. Gene map of the cow: conservation of linkage with mouse and man. J Hered. 1986 Jan-Feb;77(1):2–7. [PubMed]
  • Bernatzky R, Tanksley SD. Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics. 1986 Apr;112(4):887–898. [PMC free article] [PubMed]
  • Pichersky E, Bernatzky R, Tanksley SD, Cashmore AR. Evidence for selection as a mechanism in the concerted evolution of Lycopersicon esculentum (tomato) genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3880–3884. [PMC free article] [PubMed]
  • Pichersky E, Bernatzky R, Tanksley SD, Breidenbach RB, Kausch AP, Cashmore AR. Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b-binding proteins in Lycopersicon esculentum (tomato). Gene. 1985;40(2-3):247–258. [PubMed]
  • Chang C, Meyerowitz EM. Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1408–1412. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Beltz GA, Jacobs KA, Eickbush TH, Cherbas PT, Kafatos FC. Isolation of multigene families and determination of homologies by filter hybridization methods. Methods Enzymol. 1983;100:266–285. [PubMed]
  • Gottlieb LD. Conservation and duplication of isozymes in plants. Science. 1982 Apr 23;216(4544):373–380. [PubMed]
  • Gottlieb LD. Evidence for duplication and divergence of the structural gene for phosphoglucoisomerase in diploid species of clarkia. Genetics. 1977 Jun;86(2):289–307. [PMC free article] [PubMed]
  • Goldberg RB, Hoschek G, Kamalay JC, Timberlake WE. Sequence complexity of nuclear and polysomal RNA in leaves of the tobacco plant. Cell. 1978 May;14(1):123–131. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...