• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Sep 1993; 61(9): 3892–3900.
PMCID: PMC281091

Specific adherence of Borrelia burgdorferi extracellular vesicles to human endothelial cells in culture.


Borrelia burgdorferi produces extracellular vesicles which contain some of the outer surface proteins of the bacterium (e.g., OspA and OspB). Borrelial vesicles, isolated by differential centrifugation and filtration, were tested for the ability to bind to cultured human umbilical vein endothelial (HUVE) cells in culture. The recently described lipoprotein OspD was expressed on vesicles. Vesicles exhibited differential expression of OspB and OspD in a relationship with passage number and medium serum supplement type, respectively. Qualitative immunoblotting analyses demonstrated dose-dependent, passage number-dependent adsorption of vesicles by HUVE cells. This adsorption was demonstrated to be dependent upon a borrelial component of the vesicle and not due to the presence of minor contamination with intact spirochetes. Quantitative experiments examining inhibition of B. burgdorferi-HUVE association as a function of prior vesicle-HUVE association demonstrated dependence upon (i) a borrelial component(s) in the vesicle, (ii) low passage number, and (iii) vesicle protein concentration. However, vesicle pretreatment of the HUVE cell monolayer was not requisite for this inhibition. Vesicles from highly passaged borrelias were noninhibitory for B. burgdorferi-HUVE cell association, regardless of the serum used to supplement the medium. The use of vesicles as a tool for studying B. burgdorferi pathogenesis and/or physiology is proposed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed]
  • Barbour AG. Laboratory aspects of Lyme borreliosis. Clin Microbiol Rev. 1988 Oct;1(4):399–414. [PMC free article] [PubMed]
  • Barbour AG, Hayes SF. Biology of Borrelia species. Microbiol Rev. 1986 Dec;50(4):381–400. [PMC free article] [PubMed]
  • Barbour AG, Hayes SF, Heiland RA, Schrumpf ME, Tessier SL. A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun. 1986 May;52(2):549–554. [PMC free article] [PubMed]
  • Benach JL, Coleman JL, Garcia-Monco JC, Deponte PC. Biological activity of Borrelia burgdorferi antigens. Ann N Y Acad Sci. 1988;539:115–125. [PubMed]
  • Comstock LE, Fikrig E, Shoberg RJ, Flavell RA, Thomas DD. A monoclonal antibody to OspA inhibits association of Borrelia burgdorferi with human endothelial cells. Infect Immun. 1993 Feb;61(2):423–431. [PMC free article] [PubMed]
  • Comstock LE, Thomas DD. Characterization of Borrelia burgdorferi invasion of cultured endothelial cells. Microb Pathog. 1991 Feb;10(2):137–148. [PubMed]
  • Dorward DW, Huguenel ED, Davis G, Garon CF. Interactions between extracellular Borrelia burgdorferi proteins and non-Borrelia-directed immunoglobulin M antibodies. Infect Immun. 1992 Mar;60(3):838–844. [PMC free article] [PubMed]
  • Dorward DW, Schwan TG, Garon CF. Immune capture and detection of Borrelia burgdorferi antigens in urine, blood, or tissues from infected ticks, mice, dogs, and humans. J Clin Microbiol. 1991 Jun;29(6):1162–1170. [PMC free article] [PubMed]
  • Duray PH, Steere AC. The spectrum of organ and systems pathology in human Lyme disease. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):169–178. [PubMed]
  • Garcia-Monco JC, Fernandez Villar B, Szczepanski A, Benach JL. Cytotoxicity of Borrelia burgdorferi for cultured rat glial cells. J Infect Dis. 1991 Jun;163(6):1362–1366. [PubMed]
  • Garon CF, Dorward DW, Corwin MD. Structural features of Borrelia burgdorferi--the Lyme disease spirochete: silver staining for nucleic acids. Scanning Microsc Suppl. 1989;3:109–115. [PubMed]
  • Habicht GS, Beck G, Benach JL, Coleman JL, Leichtling KD. Lyme disease spirochetes induce human and murine interleukin 1 production. J Immunol. 1985 May;134(5):3147–3154. [PubMed]
  • Katona LI, Beck G, Habicht GS. Purification and immunological characterization of a major low-molecular-weight lipoprotein from Borrelia burgdorferi. Infect Immun. 1992 Dec;60(12):4995–5003. [PMC free article] [PubMed]
  • Kaul R, Wenman WM. Cyclic AMP inhibits developmental regulation of Chlamydia trachomatis. J Bacteriol. 1986 Nov;168(2):722–727. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lefebvre RB, Perng GC, Johnson RC. The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene. J Clin Microbiol. 1990 Jul;28(7):1673–1675. [PMC free article] [PubMed]
  • Ma Y, Sturrock A, Weis JJ. Intracellular localization of Borrelia burgdorferi within human endothelial cells. Infect Immun. 1991 Feb;59(2):671–678. [PMC free article] [PubMed]
  • Miller LC, Isa S, Vannier E, Georgilis K, Steere AC, Dinarello CA. Live Borrelia burgdorferi preferentially activate interleukin-1 beta gene expression and protein synthesis over the interleukin-1 receptor antagonist. J Clin Invest. 1992 Sep;90(3):906–912. [PMC free article] [PubMed]
  • Norris SJ, Carter CJ, Howell JK, Barbour AG. Low-passage-associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface-exposed, plasmid-encoded lipoprotein. Infect Immun. 1992 Nov;60(11):4662–4672. [PMC free article] [PubMed]
  • Radolf JD, Norgard MV, Brandt ME, Isaacs RD, Thompson PA, Beutler B. Lipoproteins of Borrelia burgdorferi and Treponema pallidum activate cachectin/tumor necrosis factor synthesis. Analysis using a CAT reporter construct. J Immunol. 1991 Sep 15;147(6):1968–1974. [PubMed]
  • Riley BS, Oppenheimer-Marks N, Hansen EJ, Radolf JD, Norgard MV. Virulent Treponema pallidum activates human vascular endothelial cells. J Infect Dis. 1992 Mar;165(3):484–493. [PubMed]
  • Sădziene A, Rosa PA, Thompson PA, Hogan DM, Barbour AG. Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization. J Exp Med. 1992 Sep 1;176(3):799–809. [PMC free article] [PubMed]
  • Schwan TG, Burgdorfer W. Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis. 1987 Nov;156(5):852–853. [PubMed]
  • Steere AC. Lyme disease. N Engl J Med. 1989 Aug 31;321(9):586–596. [PubMed]
  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. [PubMed]
  • Steere AC, Malawista SE. Cases of Lyme disease in the United States: locations correlated with distribution of Ixodes dammini. Ann Intern Med. 1979 Nov;91(5):730–733. [PubMed]
  • Szczepanski A, Furie MB, Benach JL, Lane BP, Fleit HB. Interaction between Borrelia burgdorferi and endothelium in vitro. J Clin Invest. 1990 May;85(5):1637–1647. [PMC free article] [PubMed]
  • Thomas DD, Comstock LE. Interaction of Lyme disease spirochetes with cultured eucaryotic cells. Infect Immun. 1989 Apr;57(4):1324–1326. [PMC free article] [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Whitmire WM, Garon CF. Specific and nonspecific responses of murine B cells to membrane blebs of Borrelia burgdorferi. Infect Immun. 1993 Apr;61(4):1460–1467. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...