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Plant J. Jan 2010; 61(2): 290–299.
Published online Nov 9, 2009. doi:  10.1111/j.1365-313X.2009.04054.x
PMCID: PMC2807913
NIHMSID: NIHMS152710

PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis

Abstract

Abscisic acid (ABA) mediates resistance to abiotic stress and controls developmental processes in plants. The group-A PP2Cs, of which ABI1 is the prototypical member, are protein phosphatases that play critical roles as negative regulators very early in ABA signal transduction. Because redundancy is thought to limit the genetic dissection of early ABA signalling, to identify redundant and early ABA signalling proteins, we pursued a proteomics approach. We generated YFP-tagged ABI1 Arabidopsis expression lines and identified in vivo ABI1-interacting proteins by mass-spectrometric analyses of ABI1 complexes. Known ABA signalling components were isolated including SnRK2 protein kinases. We confirm previous studies in yeast and now show that ABI1 interacts with the ABA-signalling kinases OST1, SnRK2.2 and SnRK2.3 in plants. Interestingly, the most robust in planta ABI1-interacting proteins in all LC-MS/MS experiments were nine of the 14 PYR/PYL/RCAR proteins, which were recently reported as ABA-binding signal transduction proteins, providing evidence for in vivo PYR/PYL/RCAR interactions with ABI1 in Arabidopsis. ABI1–PYR1 interaction was stimulated within 5 min of ABA treatment in Arabidopsis. Interestingly, in contrast, PYR1 and SnRK2.3 co-immunoprecipitated equally well in the presence and absence of ABA. To investigate the biological relevance of the PYR/PYLs, we analysed pyr1/pyl1/pyl2/pyl4 quadruple mutant plants and found strong insensitivities in ABA-induced stomatal closure and ABA-inhibition of stomatal opening. These findings demonstrate that ABI1 can interact with several PYR/PYL/RCAR family members in Arabidopsis, that PYR1–ABI1 interaction is rapidly stimulated by ABA in Arabidopsis and indicate new SnRK2 kinase-PYR/PYL/RCAR interactions in an emerging model for PYR/PYL/RCAR-mediated ABA signalling.

Keywords: abscisic acid, ABI1, ABI1 interacting proteins, proteome, PYR/PYL/RCAR

Introduction

The plant hormone abscisic acid (ABA) controls important abiotic stress-induced and developmental responses, including maintenance of seed dormancy, seed development, growth regulation and stomatal closure. Forward and reverse genetic analyses have led to the identification of many loci encoding ABA signalling components that function in ABA metabolism and in ABA signalling (Finkelstein et al., 2002; Nambara and Marion-Poll, 2005; Israelsson et al., 2006; Yamaguchi-Shinozaki and Shinozaki, 2006). However, genetic redundancy has hampered the identification of many important ABA signalling components. Furthermore, proteins that mediate ABA reception are a subject of present debate and insufficient evidence exists on the interactions of some of the candidate proteins with known ABA signalling components (McCourt and Creelman, 2008).

Protein phosphorylation and dephosphorylation events are important mediators of ABA signal transduction. ABA-activated SNF1-related protein kinases 2 (SnRK2s) and Ca2+ dependent protein kinases (CDPKs) are positive transducers of ABA signalling (Li et al., 2000; Mustilli et al., 2002; Yoshida et al., 2002; Mori et al., 2006; Fujii et al., 2007; Zhu et al., 2007). Furthermore calcineurin B-like proteins (CBLs) and CBL-binding protein kinases (CIPKs), encode another class of SNF1-related protein kinases that negatively affect ABA responses (Hrabak et al., 2003; Batistic and Kudla, 2009; Luan, 2009). The ABA-activated protein kinase OST1 (SnRK2.6/SnRK2E) functions as a positive regulator of ABA-induced stomatal closure (Mustilli et al., 2002; Yoshida et al., 2002). SnRK2.2 and SnRK2.3 are homologues of OST1 and are also activated by ABA and regulate ABA responses in seed germination, root growth and gene expression (Fujii et al., 2007). Recently, snrk2.2/2.3/2.6 triple mutants were shown to cause a strong ABA insensitive phenotype in seed germination, root growth and gene expression, suggesting that SnRK2.2, SnRK2.3 and OST1/SnRK2.6 have overlapping functions in ABA signalling (Fujii and Zhu, 2009; Nakashima et al., 2009).

The dominant ABA insensitive mutants abi1-1 and abi2-1 exhibit ABA insensitivity in seed germination and root growth responses (Koornneef et al., 1984). ABI1 and ABI2 encode type 2C protein phosphatases (PP2Cs) (Leung et al., 1994, 1997; Meyer et al., 1994; Rodriguez et al., 1998). The Arabidopsis genome includes more than 76 PP2C genes (Schweighofer et al., 2004; Xue et al., 2008). Signal transduction analysis in guard cells showed that abi1-1 impairs ABA signalling mechanisms including ABA activation of S-type anion channels (Pei et al., 1997), ABA-induced [Ca2+] cyt elevations (Allen et al., 1999), ABA-induced Ca2+ channel activation and ROS production (Murata et al., 2001) and ABA activation of the OST1 protein kinase in Arabidopsis (Mustilli et al., 2002). These findings led to the model that ABI1 PP2C functions very early in ABA signal transduction (Pei et al., 1997; Allen et al., 1999; Murata et al., 2001; Mustilli et al., 2002). These signal transduction studies prompted us to search for in vivo ABI1-interacting proteins via protein complex purifications in the present study to identify possible redundant early ABA signal transduction proteins.

Studies have shown that six of the nine Arabidopsis PP2Cs belonging to cluster A of the PP2Cs family (Schweighofer et al., 2004), ABI1, ABI2, HAB1, HAB2, AHG1 and PP2CA/AHG3 function as negative regulators of ABA signalling and have independent and overlapping functions (Gosti et al., 1999; Merlot et al., 2001; Leonhardt et al., 2004; Saez et al., 2004, 2006; Kuhn et al., 2006; Yoshida et al., 2006b; Nishimura et al., 2007; Rubio et al., 2009). Yeast two hybrid screens have identified substrates of ABI1, including the homeodomain transcriptional factor ATHB6 (Himmelbach et al., 2002), the protein kinases, CIPK15, CIPK20 and OST1 (Guo et al., 2002; Ohta et al., 2003; Yoshida et al., 2006a), gluthathione peroxidase3 (Miao et al., 2006) and a homologue of the yeast SWI/SNF chromatin-remodelling complex, SWI3B (Saez et al., 2008).

Recently two independent groups have reported that two Bet VI family proteins, PYR1 and RCAR1, interact with ABI1, ABI2 and HAB1 (Ma et al., 2009; Park et al., 2009). These studies have provided evidence that the recombinant protein PYR1 and the RCAR1-ABI2 protein complex, may function as ABA receptors in ABA signalling (Ma et al., 2009; Park et al., 2009). Two dimensional NMR analyses of PYR1 showed that ABA causes shifts in the conformation of PYR1 (Park et al., 2009). Furthermore, ABA stimulates the interaction of PYR1 with PP2Cs and this ABA-dependent interaction down regulates PP2C activity in vitro (Park et al., 2009). RCAR1 also negatively regulates PP2C activity after ABA treatment in vitro, though RCAR1 constitutively interacts with PP2Cs. However, whether PYR/PYL/RCAR proteins interact with ABI1 in Arabidopsis remains unknown.

Here, to identify unknown redundant early ABA signalling proteins in vivo, we pursued YFP–ABI1 complex affinity column-based purifications from Arabidopsis plants. Interestingly, we identified nine of the 14 PYR/PYL/RCAR family members as the most robustly co-purified proteins with ABI1. These data show ABI1 interaction with multiple members of PYR/PYL/RCAR family in Arabidopsis.

Here we further address the following emerging questions: (i) Does the ABI1 protein phosphatase interact with the PYR1 protein in vivo (ii) Does ABA affect this interaction in vivo and within which time frame? (iii) Does ABI1 interact with SnRK2.2, SnRK2.3 and OST1/SnRK2.6 in plants? (iv) Does PYR form complexes with these ABA signalling SnRK2 kinases and does ABA affect this interaction? and (v) Do PYR/PYL/RCAR function in ABA-induced stomatal closure and ABA inhibition of stomatal opening?

Results

Isolation of YFP–ABI1 over-expression plants

To assess further the ABA signalling cascade, we pursued experiments to identify ABI1-interacting proteins in Arabidopsis using affinity column-based protein complex purifications. We generated transgenic YFP–ABI1 and YFP Arabidopsis expression lines in an abi1-3 knockout mutant background (Saez et al., 2006). Five independent transgenic YFP–ABI1 expression lines were isolated. Western blot and fluorescence microscopy analyses showed that these Arabidopsis lines express the YFP–ABI1 and YFP proteins, respectively (Figure 1a,b). Interestingly, YFP–ABI1 expression plants showed growth and ABA insensitive phenotypes that were comparable to the dominant abi1-1 mutant. 5-week-old YFP–ABI1 plants were significantly smaller in size than control YFP expression plants (Figure 1b). Previous research has reported that ABI1–GFP over-expressing lines do not show any ABA response phenotypes compared with vector control lines (Moes et al., 2008). In our investigations, YFP–ABI1 fusion expression plants showed ABA insensitive phenotypes during seed germination, root growth and in stomatal responses compared with the control YFP expression plants confirming ABI1 function (Figure 1c–e).

Figure 1
Constitutive expression of YFP–ABI1 causes ABA insensitivity.

Identification of ABI1-interacting proteins

Using YFP–ABI1 and YFP expression in the abi1-3 knock-out background, we purified ABI1-interacting proteins. A GFP affinity column was loaded with whole protein extracts from YFP–ABI1 and control YFP expression plants grown on MS plates for 21 days with or without ABA treatment. Affinity purified protein complexes were identified by mass spectrometric analyses. The specificity of the proteins purified by YFP affinity purification was analysed in parallel negative control experiments using YFP expression plants in the abi1-3 mutant background (Tables S1 and S2). Upon silver staining, some visible bands overlapped with controls and specific bands associated that YFP–ABI1 samples were also consistently observed (Figure S1). Mass-spectrometrical analyses of five samples without ABA treatment (four independent samples and one duplicate) and five samples treated with ABA (three independent samples and two duplicates) allowed identification of proteins that associated with YFP–ABI1. Interestingly, the identified proteins included known ABA signalling components SnRK2.2, SnRK2.3, RPN10 and OST2/AHA1 (Tables 1, ,2,2, S5 and S6) (Smalle et al., 2003; Fujii et al., 2007; Merlot et al., 2007). SnRK2.2 and SnRK2.3 were not identified in any of the YFP control experiments (Table S2).

Table 1
Known ABA signalling proteins co-purified with ABI1 from Arabidopsis plants without addition of exogenous ABA
Table 2
Known ABA signalling proteins co-purified with ABI1 from Arabidopsis plants exposed to exogenous ABA

Analyses of mass-spectra identified nine proteins in the absence of exogenously applied ABA that were identified in all YFP–ABI1 experiments, but not in YFP controls (Tables 3, S3, and S7). In the presence of exogenous ABA, eleven proteins were identified in all YFP–ABI1 samples (Tables 4, S4, and S8). Initially, we named these proteins candidate ABI1 interacting proteins (ABIP). The protein with the largest sequence coverage was ABI1 (Table 3). Six of the nine proteins in samples that were not treated with exogenous ABA encoded proteins from a, at the time uncharacterized, Bet VI subfamily of proteins (Tables 3 and S3). These Bet VI family proteins including PYR1 and other close homologues have recently been independently identified as candidate ABA-binding and ABA signalling proteins (Ma et al., 2009; Park et al., 2009). Interestingly the proteins co-purified with ABI1 with the highest total and unique sequence coverage and number of unique peptides and spectrum count were all members of the PYR/PYL/RCAR protein family (Figure 2a, Tables 3, S3 and S7). Two additional proteins from the 14 member PYR/PYL/RCAR protein family were identified in three or four of the five experiments (Table 3, PYL7 and PYL10). Moreover, in ABA-treated samples eight PYR/PYL/RCAR proteins had the largest total and unique sequence coverage and number of unique peptides and spectrum count of the isolated ABI1-interacting proteins and were identified in all five LC-MS/MS experiments (Tables 4, S4 and S8). As Bet VI proteins were the most significantly and consistently represented proteins from among all sequenced protein peptides in YFP–ABI1 samples, but not in YFP control samples (Table S2), these findings provide evidence that the ABI1 protein phosphatase can form complexes with several of the 14 PYR/PYL/RCAR Bet VI family members in vivo (Figure 2a, Tables 3, ,4,4, S1, S3, S4, S7 and S8).

Table 3
Candidate ABI1-interacting proteins with the largest mass-spectrometrical sequence coverage from five LC-MS/MS experiments analyzing ABI1 complexes isolated from Arabidopsis in the absence of exogenous ABA
Table 4
Candidate ABI1-interacting proteins with the largest mass-spectrometrical sequence coverage from five LC-MS/MS experiments analyzing Arabidopsis ABI1 complexes exposed to exogenous ABA
Figure 2
ABA causes ABI1–PYR1 interaction in Arabidopsis.

ABI1 interacts with PYR1 in Arabidopsis

It was recently shown that ABA enhances the interaction of YFP–ABI1 with HA–PYR1 transiently expressed in Nicotiana benthamiana leaves (Park et al., 2009). To assess whether these interactions occur in vivo in Arabidopsis, we performed co-immunoprecipitation experiments in stably transformed YFP–ABI1 and YFP control expression lines using a PYR1 antibody. YFP–ABI1 interacted with PYR1 in Arabidopsis with a strong enhancement upon application of 100 μm exogenous ABA for 48 h (Figure S2). Subsequently, co-immunoprecipitation experiments were performed three h after exogenous application of 0, 1, 10 and 100 μm ABA. ABA caused a clear enhancement in ABI1–PYR1 interaction in vivo (Figure 2b). To determine the timing of this ABA response in Arabidopsis, co-immunoprecipitation experiments were performed 0, 5, 10, 30 or 60 min after application of exogenous ABA. These experiments showed a clear ABA induction of a YFP–ABI1 and PYR1 interaction within 5 min of ABA treatment in vivo (Figure 2c).

PP2Cs, PYR1 and SnRK2s interact with each other in ABA signalling

ABI1 has been reported to interact with OST1/SnRK2E/SnRK2.6 in yeast (Yoshida et al., 2006a). However it remains unknown whether this interaction occurs in plant cells and whether ABI1 interacts with other SnRK2s. It also remains unknown whether any ABI1–SnRK2 interactions are regulated by ABA similar to the ABI1–PYR1 interaction. As SnRK2.2 and SnRK2.3 were co-purified with ABI1 in our affinity column purification experiments (Tables 1 and and2),2), we co-expressed YFP–ABI1 with either HA–SnRK2.2, HA–SnRK2.3 or HA–OST1/SnRK2.6 in N. benthamiana and performed co-immunoprecipitation experiments. YFP–ABI1 constitutively interacted with HA–SnRK2.2, HA–SnRK2.3 and HA–OST1/SnRK2.6 both in the absence and presence of exogenously applied ABA (Figure 3).

Figure 3
ABI1 PP2C co-immunoprecipitates with all three ABA signalling SnRK2 kinases in plants.

We analysed whether PYR1 might co-purify with SnRK2s. We co-expressed YFP–PYR1 with either HA–SnRK2.3 or HA–OST1/SnRK2.6 in N. benthamiana and performed co-immunoprecipitation experiments. Interestingly, YFP–PYR1 co-immunoprecipitated with HA–SnRK2.3 both in the presence and absence of ABA, but not detectably or very weakly with HA–OST1/SnRK2.6 (Figure 4).

Figure 4
Interaction of PYR1 with SnRK2.3 and OST1.

pyr1/pyl1/pyl2/pyl4 quadruple mutant plants exhibit ABA insensitive phenotype in guard cells

The abi1-1 protein impairs ABA signalling upstream of known early ABA responses in Arabidopsis guard cells (Pei et al., 1997; Allen et al., 1999; Murata et al., 2001; Mustilli et al., 2002). A pyr1/pyl1/pyl2/pyl4 quadruple mutant was recently shown to cause strong ABA insensitivities in seed germination, root growth and ABA-induced gene expression (Park et al., 2009). Whether PYR/PYL/RCAR genes mediate ABA response in guard cells remains unknown. Public microarray data show that the PYR1, PYL1, PYL2, PYL4, PYL5, PYL7 and PYL8 mRNAs are highly expressed in guard cells and seedlings (Leonhardt et al., 2004; Goda et al., 2008; Yang et al., 2008) (Figure S4). Stomatal response analyses were performed with pyr1/pyl1/pyl2/pyl4 quadruple mutant plants. pyr1/pyl1/pyl2/pyl4 quadruple mutants showed a strong ABA insensitive phenotype in double-blinded ABA-induced stomatal closing and ABA inhibition of stomatal opening analyses (Figures 5a,b and S5). Studies have provided evidence that Ca2+ signalling functions downstream of the abi1-1 and abi2-1 PP2Cs in guard cells (Allen et al., 1999; Murata et al., 2001). Experiments were pursued to determine whether pyr1/pyl1/pyl2/pyl4 quadruple mutant plants impair Ca2+-induced stomatal closing. Repetitive addition and removal of Ca2+ to the extracellular solution bathing leaf epidermes while shifting the K+ equilibrium potential in guard cells, results in Ca2+-induced stomatal closure (Allen et al., 2001). Such imposed extracellular Ca2+ pulses caused similar Ca2+-reactive and Ca2+-programmed stomatal closure responses in both pyr1/pyl1/pyl2/pyl4 quadruple mutant and wild type epidermes (Figure 5c). These results suggest that these PYR/PYL/RCAR proteins are critical for ABA signalling in guard cells upstream of Ca2+ signalling.

Figure 5
ABA-induced stomatal closure and ABA-inhibition of stomatal opening but not Ca2+ -induced stomatal closure are impaired in pyr1pyl1pyl2pyl4 quadruple mutant plants.

Discussion

Studies have suggested that ABI1 functions upstream of all analysed events in early ABA signal transduction in guard cells (Pei et al., 1997; Allen et al., 1999; Murata et al., 2001; Mustilli et al., 2002). We hypothesized that redundant proteins may act upstream of ABI1, because forward genetic screens had not identified other upstream loci. Our early attempts to co-purify ABI1-complexed proteins from Arabidopsis, showed that additional purification steps led to loss of many ABI1-associated proteins (I. Mori & J. I. Schroeder unpublished data). A rapid purification method using GFP affinity column-affinity purification, as reported here, was more fruitful for isolating redundant ABA signalling proteins in vivo (Tables 14, S1S4, S7, S8). Here we identify nine of the 14 PYR/PYL/RCAR gene family proteins as in vivo ABI1-co-purified proteins with the largest sequence coverage and number of unique peptides and spectrum count of all identified proteins (Tables 3, ,4,4, S3, S4, S7 and S8; Figures 2 and and3).3). After identifying these Bet VI family proteins as the main co-purified proteins in ABI1 complexes from Arabidopsis, we learned that PYR1 had been independently isolated in a chemical genetics screen as a candidate ABA signalling protein (Park et al., 2009). In the absence of ABA, PYR1 and PYL4 proteins were co-purified as ABI1-interacting proteins (Tables 3, S3 and S7), although ABA enhanced the interaction of ABI1–PYR1 (Figure 2) and HAB1–PYL4 (Park et al., 2009). Co-purification results in the absence of exogenously applied ABA may be due to the known endogenous ABA levels in Arabidopsis plants (Nambara and Marion-Poll, 2005). On the other hand, ABI1 seems to interact constitutively with PYL5 to PYL9 in Arabidopsis, in the absence of ABA, based on yeast two hybrid data (Ma et al., 2009; Park et al., 2009). Over-expression of several other PP2Cs causes ABA insensitive phenotypes in seed germination stages (Saez et al., 2004; Kuhn et al., 2006; Yoshida et al., 2006b; Nishimura et al., 2007). YFP–ABI1 expression plants showed ABA insensitive phenotypes during seed germination, root growth and in stomatal responses compared with the control YFP expression plants (Figure 1c–e).

In addition to the identification of PYR/PYL/RCAR proteins as the major proteins co-purified with ABI1 from Arabidopsis plants, the present study reports several further relevant new findings: (i) A new PYR1 antibody was used and showed that the ABI1 protein phosphatase interacts with the PYR1 protein in vivo (ii) ABA stimulates this interaction in Arabidopsis within 5 min of ABA exposure. (iii) ABI1 co-immunoprecipitates with the SnRK2.2, SnRK2.3 and OST1/SnRK2.6 protein kinases in plants, extending previous yeast two hybrid findings (Yoshida et al., 2006a). (iv) PYR1 forms complexes with these ABA signalling SnRK2 kinases in plants both in the presence and absence of ABA. (v) PYR/PYL/RCAR genes are critical for ABA-induced stomatal closure and ABA inhibition of stomatal opening.

Protein phosphorylation and dephosphorylation events in ABA signalling

The protein kinase OST1/SnRK2.6 is phosphorylated within 5 min of ABA application (Mustilli et al., 2002; Yoshida et al., 2006a). ABA binds to PYR1 and RCAR1–ABI2 thus down-regulating PP2C activities (Ma et al., 2009; Park et al., 2009). In addition, PYR/PYL/RCAR genes are required for ABA activation of the SnRK2 kinases (Park et al., 2009). Here ABA is shown to cause PYR1–ABI1 interaction in Arabidopsis, whereas PYR1 forms a constitutive complex with the SnRK2.3 kinase both in the absence and presence of ABA (Figure 4). Whether PYR1 directly binds to SnRK2s remains to be determined. Furthermore, ABI1 constitutively interacts with the SnRK2.2, SnRK2.3 and OST1/SnRK2.6 kinases in planta (Figure 3). These co-immunoprecipitation results support the hypothesis that these major ABA signalling components, PYR/PYL/RCARs, PP2Cs and SnRK2s, may form an ABA signalosome complex, rather than forming a simple sequential PYR1→ABI1→SnRK2 signalling pathway. An initial sequential model (Park et al., 2009) would predict that SnRK2 kinases may only enter into a complex with PYR1 after ABA exposure.

One of the substrates of SnRK2s is a b-ZIP type transcription factor ABRE/ABF (Furihata et al., 2006; Fujii et al., 2007). Arabidopsis ost2 and slac1 mutants show strong ABA insensitive phenotypes in guard cells and act downstream of the OST1 kinase (Li et al., 2000; Merlot et al., 2007; Negi et al., 2008; Vahisalu et al., 2008). We isolated OST2/AHA1 as a candidate ABI1-interacting protein (Tables 1 and and2),2), which would be consistent with the model that the ABA signalling PP2Cs regulate multiple downstream signalling proteins (Pei et al., 1997; Allen et al., 1999; Murata et al., 2001; Furihata et al., 2006; Kuhn et al., 2006; Nishimura et al., 2007; Saez et al., 2008;).

PYR/PYL/RCAR family members regulate ABA signalling in guard cells

Several ABA receptors have been reported to impair ABA-induced stomatal closure; CHLH was reported as an ABA receptor but only one stable and available genetic allele cch (Mochizuki et al., 2001) showed a strong ABA insensitive phenotype (Shen et al., 2006). Recently, double mutants in the candidate ABA receptors, GTG1 and GTG2, were shown to cause ABA insensitivity in seed germination, root growth, stomatal movements and ABA-induced gene expression (Pandey et al., 2009). For ABA-induced stomatal closure, gtg1gtg2 double mutants exhibited a limited insensitivity to ABA compared with WT, suggesting that additional ABA receptors are needed to perceive ABA and transduce the ABA signal independently. More recently, the PYR/PYL/RCAR family proteins were identified as new ABA binding and signalling proteins (Ma et al., 2009; Park et al., 2009). Here we show that pyr1/pyl1/pyl2/pyl4 quadruple mutant plants exhibit a strong ABA insensitive phenotype in ABA-induced stomatal closing and ABA inhibition of stomatal opening but not in Ca2+-induced stomatal closing (Figure 5), suggesting that PYR1/PYL1/PYL2/PYL4 function upstream of Ca2+ in ABA signalling and likely also upstream of Ca2+-independent signalling (Allan et al., 1994; Siegel et al., 2009). Furthermore PYR1/PYL1/PYL2/PYL4 mediate ABA signalling not only in seed germination and seedling stages (Park et al., 2009) but also in guard cells (Figure 5).

In conclusion, in vivo affinity column purifications of ABI1 complex proteins provide evidence that the PYR/PYL/RCAR family of proteins are the most abundant and robust in vivo ABI1-complexed interactors in Arabidopsis. ABA rapidly stimulates PYR1–ABI1 interaction in Arabidopsis and additional findings indicate the possible assembly of an early ABA signalosome.

Experimental procedures

Plant materials and growth conditions

The Arabidopsis thaliana (L.) Heynh. ecotype Columbia (Col) was used in this study. Plant growth conditions, seed germination and root elongation assays were performed as described previously (Nishimura et al., 2004; Mori et al., 2006). ABA-induced and repetitive Ca2+ pulse-stomatal movement responses and apertures were analysed as described previously (Vahisalu et al., 2008). For ABA inhibition of stomatal opening, to pre-close stomata, intact leaf epidermes attached to a cover slip were pre-incubated in buffer (10 mm KCl, 7.5 mm iminodiacetic acid and 10 mm MES/Tris, pH 6.2) for 3 h in the dark and exposed to the indicated ABA concentrations in the light (100 μmol m−2 s−1).

Cloning and construction of transgenic YFP–ABI1 Arabidopsis lines

The full length ABI1 cDNA was cloned into pDONR vector (Invitrogen, http://www.invitrogen.com) and sequenced and then transferred to the destination vectors pH35YG and pEarleyGate 201 by Gateway LR recombination reaction (Invitrogen). Agrobacterium tumefaciens strain GV3101 was used to transform the abi1-3 T-DNA insertion disruption line and wild-type Col-0 with plants by the floral dip method (Clough and Bent, 1998). Transgenic plant lines were screened for hygromycin resistance and homozygous T3 lines were isolated.

Affinity column purification of YFP–ABI1 complexed proteins

Identification of proteins by mass spectrometry is described in the Experimental procedures S1.

Co-immunoprecipitation in Arabidopsis and Nicotiana benthamiana

For protein extractions, 3-week-old seedlings (1 g) grown on horizontal MS plates were incubated for 2 h in water before ABA treatment. Co-immunoprecipitations were performed using Dynabeads Protein G (Invitrogen) captured with anti-GFP antibody (Abcam, http://www.abcam.com) according to the manufacture’s specifications. Co-immunoprecipitations in N. benthamiana were performed as described previously without modifications (Park et al., 2009).

Acknowledgments

We thank Drs M. Böhmer and D.G. Mendoza-Cozatl for technical advice, Dr T.H. Kim for critical comments and reading of the manuscript and Drs I. Mori and M. Ghassemian (UCSD) for pursuing early abi1-1 and ABI1 complex purification attempts in the Schroeder laboratory. We are also grateful to the Arabidopsis Biological Resource Center (ABRC) and Dr T. Demura (NAIST) for the pH35YG plasmid. Requests for the pyr1pyl1pyl2pyl4 quadruple mutant seeds should be sent to Sean.cutler/at/ucr.edu. J.C. is an investigator of the Howard Hughes Medical Institute. This research was supported by NIH (GM060396-ES010337) and NSF (MCB0417118-MCB0918220) grants to J.I.S., NSF (IOS-0820508) to S.R.C., NIH (R01 GM052413) to J.C. and NIH (P41 RR011823) to J.R.Y.

Author contributions: N.N. performed experiments in Figures 1, ,2,2, ,33 and and44 and analysed mass spectrometry data. A.S. and J.R.Y. performed mass spectrometry and A.S., P.C., N.N. and J.I.S. analysed mass spectrometry data. K.N. and J.C. provided GFP antibody and assisted with affinity purifications. S.Y.P. and S.R.C. generated PYR1 antibody and pyr1pyl1pyl2pyl4 quadruple mutant seeds. A.W., S.L. and D.F.C. performed experiments in Figures 1(d,e) and and5.5. J.I.S. conceived of the project and J.I.S. and N.N. developed experimental designs. N.N. and J.I.S. wrote the paper.

SUPPORTING INFORMATION

Additional Supporting Information may be found in the online version of this article:

Figure S1. Identification of ABI1-interacting proteins in Arabidopsis.

Figure S2. ABA-induced interaction between ABI1 and PYR1 in Arabidopsis.

Figure S3. Tandem mass spectrum of peptides from proteins ABIP8 and ABIP11 listed in Tables S3 and S4 that showed one unique sequence to the corresponding proteins.

Figure S4. Transcription profiles of ABI1, PYR1/PYLs and SnRK2s in seedlings, guard cells and mesophyll cells.

Figure S5. ABA-induced stomatal closure isimpaired in pyr1pyl1-pyl2pyl4 quadruple mutant plants.

Table S1. List of candidate ABI1-interacting proteins in YFP–ABI1 plants derived from LC-MS/MS data.

Table S2. List of candidate YFP-interacting proteins in control YFP plants derived from LC-MS/MS data.

Table S3. The number of unique and total peptides and spectrum count of candidate ABI1-interacting proteins co-purified with ABI1 from Arabidopsis plants in the absence of exogenous ABA.

Table S4. The number of unique and total peptides and spectrum count of candidate ABI1-interacting proteins co-purified with ABI1 from plants exposed to exogenous ABA.

Table S5. The unique sequence coverage of known ABA signalling proteins co-purified with ABI1 from Arabidopsis plants without addition of exogenous ABA.

Table S6. The unique sequence coverage of known ABA signalling proteins co-purified with ABI1 from Arabidopsis plants exposed to exogenous ABA.

Table S7. The unique sequence coverage of candidate ABI1-interacting proteins co-purified with ABI1 from Arabidopsis plants in the absence of exogenous ABA.

Table S8. The unique sequence coverage of candidate ABI1-interacting proteins co-purified with ABI1 from plants exposed to exogenous ABA.

Experimental procedures S1.

Purification of YFP–ABI1 interacting proteins by affinity column purification methods.

Multidimensional protein identification technology (MudPIT).

Analysis of tandem mass spectra.

PYR1 expression and antiserum preparation.

Construction of the erecta+ pyr1/pyl1/pyl2/pyl4 quadruple mutant.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

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