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Proc Natl Acad Sci U S A. May 1988; 85(10): 3494–3498.
PMCID: PMC280238

Isolation and characterization of cDNA clones encoding human liver glutamate dehydrogenase: evidence for a small gene family.


We have isolated a series of human liver cDNA clones encoding glutamate dehydrogenase. The cDNA-derived protein sequence specifies a single 558-amino acid long polypeptide including a cleavable signal sequence of 53 amino acids. Blotting analysis of RNA from human, monkey, and rabbit showed that glutamate dehydrogenase mRNA is present in various amounts in all tissues tested. Glutamate dehydrogenase mRNAs are of four sizes and are found in different ratios in different tissues; the predominant ones are approximately 3.5 and approximately 2.9 kilobases. Blot hybridization of human genomic DNA to nonoverlapping cDNA fragments revealed multiple bands, many of which hybridize with two or more probes in a manner inconsistent with the existence of a single GLUD gene. Moreover, two separate 36-base synthetic oligonucleotides corresponding to the coding region hybridize to multiple genomic fragments, confirming the existence of more than one GLUD-related gene in human.

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  • Plaitakis A, Nicklas WJ, Desnick RJ. Glutamate dehydrogenase deficiency in three patients with spinocerebellar syndrome. Ann Neurol. 1980 Apr;7(4):297–303. [PubMed]
  • Dennis SG, Clark JB. The synthesis of glutamate by rat brain mitochondria. J Neurochem. 1978 Sep;31(3):673–680. [PubMed]
  • Yu AC, Schousboe A, Hertz L. Metabolic fate of 14C-labeled glutamate in astrocytes in primary cultures. J Neurochem. 1982 Oct;39(4):954–960. [PubMed]
  • Berl S. Cerebral amino acid metabolism in hepatic coma. Exp Biol Med. 1971;4:71–84. [PubMed]
  • Fonnum F. Glutamate: a neurotransmitter in mammalian brain. J Neurochem. 1984 Jan;42(1):1–11. [PubMed]
  • Olney JW, Ho OL, Rhee V. Cytotoxic effects of acidic and sulphur containing amino acids on the infant mouse central nervous system. Exp Brain Res. 1971;14(1):61–76. [PubMed]
  • McGeer EG, McGeer PL. Duplication of biochemical changes of Huntington's chorea by intrastriatal injections of glutamic and kainic acids. Nature. 1976 Oct 7;263(5577):517–519. [PubMed]
  • Rothman S. Synaptic release of excitatory amino acid neurotransmitter mediates anoxic neuronal death. J Neurosci. 1984 Jul;4(7):1884–1891. [PubMed]
  • Wieloch T. Hypoglycemia-induced neuronal damage prevented by an N-methyl-D-aspartate antagonist. Science. 1985 Nov 8;230(4726):681–683. [PubMed]
  • Plaitakis A, Berl S, Yahr MD. Neurological disorders associated with deficiency of glutamate dehydrogenase. Ann Neurol. 1984 Feb;15(2):144–153. [PubMed]
  • Colon AD, Plaitakis A, Perakis A, Berl S, Clarke DD. Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain. J Neurochem. 1986 Jun;46(6):1811–1819. [PubMed]
  • Julliard JH, Smith EL. Partial amino acid sequence of the glutamate dehydrogenase of human liver and a revision of the sequence of the bovine enzyme. J Biol Chem. 1979 May 10;254(9):3427–3438. [PubMed]
  • Cladaras C, Hadzopoulou-Cladaras M, Nolte RT, Atkinson D, Zannis VI. The complete sequence and structural analysis of human apolipoprotein B-100: relationship between apoB-100 and apoB-48 forms. EMBO J. 1986 Dec 20;5(13):3495–3507. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Goossens M, Kan YY. DNA analysis in the diagnosis of hemoglobin disorders. Methods Enzymol. 1981;76:805–817. [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Zannis VI, Kurnit DM, Breslow JL. Hepatic apo-A-I and apo-E and intestinal apo-A-I are synthesized in precursor isoprotein forms by organ cultures of human fetal tissues. J Biol Chem. 1982 Jan 10;257(1):536–544. [PubMed]
  • Zannis VI, Cole FS, Jackson CL, Kurnit DM, Karathanasis SK. Distribution of apolipoprotein A-I, C-II, C-III, and E mRNA in fetal human tissues. Time-dependent induction of apolipoprotein E mRNA by cultures of human monocyte-macrophages. Biochemistry. 1985 Jul 30;24(16):4450–4455. [PubMed]
  • Banner C, Silverman S, Thomas JW, Lampel KA, Vitkovic L, Huie D, Wenthold RJ. Isolation of a human brain cDNA for glutamate dehydrogenase. J Neurochem. 1987 Jul;49(1):246–252. [PubMed]
  • Knowles BB, Howe CC, Aden DP. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science. 1980 Jul 25;209(4455):497–499. [PubMed]
  • Sundström C, Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int J Cancer. 1976 May 15;17(5):565–577. [PubMed]
  • Kozak M. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol. 1987 Oct;7(10):3438–3445. [PMC free article] [PubMed]
  • Mohana Rao JK, Argos P. A conformational preference parameter to predict helices in integral membrane proteins. Biochim Biophys Acta. 1986 Jan 30;869(2):197–214. [PubMed]
  • Mihara K, Omura T, Harano T, Brenner S, Fleischer S, Rajagopalan KV, Blobel G. Rat liver L-glutamate dehydrogenase, malate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, and sulfite oxidase are each synthesized as larger precursors by cytoplasmic free polysomes. J Biol Chem. 1982 Apr 10;257(7):3355–3358. [PubMed]
  • Blobel G. Intracellular protein topogenesis. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496–1500. [PMC free article] [PubMed]
  • Maeda N, Bliska JB, Smithies O. Recombination and balanced chromosome polymorphism suggested by DNA sequences 5' to the human delta-globin gene. Proc Natl Acad Sci U S A. 1983 Aug;80(16):5012–5016. [PMC free article] [PubMed]
  • Hanauer A, Mattei MG, Mandel JL. Presence of a TaqI polymorphism in the human glutamate dehydrogenase (GLUD) gene on chromosome 10. Nucleic Acids Res. 1987 Aug 11;15(15):6308–6308. [PMC free article] [PubMed]

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