• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Mar 1988; 85(5): 1544–1548.
PMCID: PMC279809

Merosin, a protein specific for basement membranes of Schwann cells, striated muscle, and trophoblast, is expressed late in nerve and muscle development.

Abstract

We have identified a tissue-specific basement membrane-associated protein by using monoclonal antibodies prepared against a protein fraction of human placenta. In immunofluorescence, the monoclonal antibodies stained basement membranes of Schwann cells, striated muscle, and trophoblast, whereas no reaction was seen with any other basement membrane or tissue structure. In antibody-affinity chromatography of proteolytic digests of human placenta, a 65-kDa polypeptide was bound by these monoclonal antibodies. Rabbit antisera and monoclonal antibodies raised against the isolated 65-kDa polypeptide stained human and monkey tissues identically to the original monoclonal antibodies and reacted with an 80-kDa polypeptide in tissue extracts prepared without proteolysis. The 65-kDa and 80-kDa polypeptides were shown to be immunologically distinct from laminin, type IV collagen, fibronectin, and major serum proteins. They presumably represent a novel basement membrane-associated protein, which we have named merosin. No merosin immunoreactivity could be detected in cultures of any of 28 established cell lines. In developing mouse tissues, merosin staining first appeared at the newborn stage. The restricted tissue distribution and late developmental appearance of merosin suggest that the protein has a tissue-specific function associated with a high level of differentiation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Vracko R. Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol. 1974 Nov;77(2):314–346. [PMC free article] [PubMed]
  • Timpl R, Rohde H, Robey PG, Rennard SI, Foidart JM, Martin GR. Laminin--a glycoprotein from basement membranes. J Biol Chem. 1979 Oct 10;254(19):9933–9937. [PubMed]
  • Chung AE, Jaffe R, Freeman IL, Vergnes JP, Braginski JE, Carlin B. Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. Cell. 1979 Feb;16(2):277–287. [PubMed]
  • Dehm P, Kefalides NA. The collagenous component of lens basement membrane. The isolation and characterization of an alpha chain size collagenous peptide and its relationship to newly synthesized lens components. J Biol Chem. 1978 Oct 10;253(19):6680–6686. [PubMed]
  • Carlin B, Jaffe R, Bender B, Chung AE. Entactin, a novel basal lamina-associated sulfated glycoprotein. J Biol Chem. 1981 May 25;256(10):5209–5214. [PubMed]
  • Hassell JR, Robey PG, Barrach HJ, Wilczek J, Rennard SI, Martin GR. Isolation of a heparan sulfate-containing proteoglycan from basement membrane. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4494–4498. [PMC free article] [PubMed]
  • MIDGLEY AR, Jr, PIERCE GB., Jr IMMUNOHISTOCHEMICAL ANALYSIS OF BASEMENT MEMBRANES OF THE MOUSE. Am J Pathol. 1963 Dec;43:929–943. [PMC free article] [PubMed]
  • Wan YJ, Wu TC, Chung AE, Damjanov I. Monoclonal antibodies to laminin reveal the heterogeneity of basement membranes in the developing and adult mouse tissues. J Cell Biol. 1984 Mar;98(3):971–979. [PMC free article] [PubMed]
  • Hessle H, Sakai LY, Hollister DW, Burgeson RE, Engvall E. Basement membrane diversity detected by monoclonal antibodies. Differentiation. 1984;26(1):49–54. [PubMed]
  • Fitch JM, Linsenmayer TF. Monoclonal antibody analysis of ocular basement membranes during development. Dev Biol. 1983 Jan;95(1):137–153. [PubMed]
  • Leivo I, Engvall E. C3d fragment of complement interacts with laminin and binds to basement membranes of glomerulus and trophoblast. J Cell Biol. 1986 Sep;103(3):1091–1100. [PMC free article] [PubMed]
  • Leu FJ, Engvall E, Damjanov I. Heterogeneity of basement membranes of the human genitourinary tract revealed by sequential immunofluorescence staining with monoclonal antibodies to laminin. J Histochem Cytochem. 1986 Apr;34(4):483–489. [PubMed]
  • McMahan UJ, Sanes JR, Marshall LM. Cholinesterase is associated with the basal lamina at the neuromuscular junction. Nature. 1978 Jan 12;271(5641):172–174. [PubMed]
  • Sakai LY, Keene DR, Morris NP, Burgeson RE. Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol. 1986 Oct;103(4):1577–1586. [PMC free article] [PubMed]
  • Wewer U, Albrechtsen R, Manthorpe M, Varon S, Engvall E, Ruoslahti E. Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis. J Biol Chem. 1983 Oct 25;258(20):12654–12660. [PubMed]
  • Leivo I, Vaheri A, Timpl R, Wartiovaara J. Appearance and distribution of collagens and laminin in the early mouse embryo. Dev Biol. 1980 Apr;76(1):100–114. [PubMed]
  • Albrechtsen R, Nielsen M, Wewer U, Engvall E, Ruoslahti E. Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res. 1981 Dec;41(12 Pt 1):5076–5081. [PubMed]
  • Ekblom P, Miettinen M, Rapola J, Foidart JM. Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues. Histochemistry. 1982;75(3):301–307. [PubMed]
  • Engvall E, Davis GE, Dickerson K, Ruoslahti E, Varon S, Manthorpe M. Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site. J Cell Biol. 1986 Dec;103(6 Pt 1):2457–2465. [PMC free article] [PubMed]
  • Engvall E, Krusius T, Wewer U, Ruoslahti E. Laminin from rat yolk sac tumor: isolation, partial characterization, and comparison with mouse laminin. Arch Biochem Biophys. 1983 Apr 15;222(2):649–656. [PubMed]
  • Miller EJ, Rhodes RK. Preparation and characterization of the different types of collagen. Methods Enzymol. 1982;82(Pt A):33–64. [PubMed]
  • Hessle H, Engvall E. Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies. J Biol Chem. 1984 Mar 25;259(6):3955–3961. [PubMed]
  • Engvall E, Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer. 1977 Jul 15;20(1):1–5. [PubMed]
  • Engvall E. Enzyme immunoassay ELISA and EMIT. Methods Enzymol. 1980;70(A):419–439. [PubMed]
  • Krusius T, Gehlsen KR, Ruoslahti E. A fibroblast chondroitin sulfate proteoglycan core protein contains lectin-like and growth factor-like sequences. J Biol Chem. 1987 Sep 25;262(27):13120–13125. [PubMed]
  • Chiu AY, Sanes JR. Development of basal lamina in synaptic and extrasynaptic portions of embryonic rat muscle. Dev Biol. 1984 Jun;103(2):456–467. [PubMed]
  • Harrisson F, Van Hoof J, Vanroelen C, Foidart JM. Masking of antigenic sites of fibronectin by glycosaminoglycans in ethanol-fixed embryonic tissue. Histochemistry. 1985;82(2):169–174. [PubMed]
  • Chiu AY, Matthew WD, Patterson PH. A monoclonal antibody that blocks the activity of a neurite regeneration-promoting factor: studies on the binding site and its localization in vivo. J Cell Biol. 1986 Oct;103(4):1383–1398. [PMC free article] [PubMed]
  • Schafer DA, Stockdale FE. Identification of sarcolemma-associated antigens with differential distributions on fast and slow skeletal muscle fibers. J Cell Biol. 1987 Apr;104(4):967–979. [PMC free article] [PubMed]
  • WIRSEN C, LARSSON KS. HISTOCHEMICAL DIFFERENTIATION OF SKELETAL MUSCLE IN FOETAL AND NEWBORN MICE. J Embryol Exp Morphol. 1964 Dec;12:759–767. [PubMed]
  • Jacque C, Delassalle A, Raoul M, Baumann N. Myelin basic protein deposition in the optic and sciatic nerves of dysmyelinating mutants quaking, jimpy, Trembler, mld, and shiverer during development. J Neurochem. 1983 Nov;41(5):1335–1340. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats: