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Eur Surg Res. Sep 2009; 43(3): 291–297.
Published online Aug 7, 2009. doi:  10.1159/000232939
PMCID: PMC2790741

A Prospective Bicenter Study Investigating the Diagnostic Value of Procalcitonin in Patients with Acute Appendicitis



Procalcitonin (PCT) is an established laboratory marker for disease severity in patients with infection and sepsis. In addition, PCT has been shown to be an effective marker for a limited number of localized infections. However, whether or not PCT has any diagnostic value for acute appendicitis, still remains unclear. The purpose of this prospective bicenter study was, therefore, to determine whether or not the PCT levels in the serum of patients with acute appendicitis have any diagnostic value.


This prospective study included 103 patients who received an appendectomy, based on the clinical diagnosis of acute appendicitis, in a surgical department of an academic teaching hospital in Germany or in a county hospital in Spain. White blood cell count (WBC), C-reactive protein (CRP) and procalcitonin (PCT) values were determined preoperatively. All appendectomy specimens were sent for routine histopathological evaluation. Based on this information, the patients were assigned to 1 of 5 groups that reflected the severity of the appendicitis.


Of the 103 patients who were included in the study, 98 had appendicitis. Fourteen (14.3%) showed an increase in PCT values. Of those 14, 4 had a serum PCT >0.5 ng/ml, 9 had a PCT value >2–10 ng/ml and 1 had a PCT value >10 ng/ml. The sensitivity of PCT was calculated to be 0.14. The mean WBC value was 13.0/nl (± 5.2, 3.4–31), and for CRP it was 8.8 mg/dl (± 13, 0–60.2). The values of CRP, WBC and PCT increased with the severity of the appendicitis.


PCT is potentially increased in rare cases of severe inflammation and, in particular, after appendiceal perforation or gangrenous appendicitis. However, its remarkably low sensitivity prohibits its routine use for the diagnosis of appendicitis.

Key Words: Procalcitonin, Appendicitis, Diagnostic


Acute appendicitis is still the most frequent cause of right lower quadrant pain and the most common acute surgical condition of the abdomen. Acute appendicitis remains one of the most serious abdominal illnesses. It is a pathological condition that requires immediate surgical treatment. Its reported overall mortality rate is 0.3%, which rises considerably in cases of perforation (6.5%), older patients (5.5%) or neonates (80%) [1]. Ultimately, advanced bacterial peritonitis following perforation of the inflamed appendix can reach a natural mortality rate as high as 80–100%, which underlines the potential life-threatening danger that is still caused by this common disease.

The frequency of appendicitis and the high rates of negative appendectomies, reported to be between 9 and 44%, have led to continuing efforts to develop new diagnostic methods that have the highest possible sensitivity and specificity in order to reduce the rate of negative appendectomies [2].

Despite the multiple modern diagnostic tools that are available, diagnosis of acute appendicitis is still primarily done by history and physical examination. Additional diagnostic measures like temperature measurement, urinalysis and laboratory tests are routinely performed.

The clinical diagnosis of appendicitis remains a challenge as a variety of differential diagnoses have to be considered as causative for right lower quadrant pain. The laboratory tests that are useful for the diagnosis of acute appendicitis are white blood cell count (WBC), C-reactive protein (CRP) and erythrocyte sedimentation rate. Another laboratory value that has recently received considerable attention for the early diagnosis of bacterial infection and sepsis is procalcitonin (PCT). In recent studies, PCT has clearly been shown to be one of the most important biochemical indicators that closely correlate with the severity of the inflammatory host response to microbial infections [3]. The aim of this open, prospective bicenter study is, therefore, to investigate whether or not PCT offers an accurate diagnostic tool for acute appendicitis.


This study originates from a surgical department of an academic teaching hospital (Augusta Krankenanstalt, Ruhr University Bochum, Germany) and the surgical department of a county general hospital (Hospital de Igualada, Consorci Sanitari de l’Anoia, Igualada, Spain). It was conducted within the framework of the declaration of Helsinki and approved by the Ethics Review Board of the Ruhr-University Bochum, Germany (registration No.: 3133-08, ClinicalTrials.gov identifier: NCT00630071). Informed consent was obtained from the study subjects.


All patients who were clinically diagnosed as having acute appendicitis between June 2007 and June 2008 were included in the study.

The exclusion criteria were prior antibiotic therapy, an age <10 years, pregnancy and other known inflammatory conditions or infections.

The diagnosis was made by the attending surgeon, based upon the patient's history and clinical examination. When possible, laparoscopic appendectomy was favored. In some rare cases, when the laparoscopic approach was not possible, the appendectomy was performed conventionally. All appendectomy specimens were sent for routine histopathological evaluation.

Following the study by Kafetzis et al. [4], who investigated PCT as a predictor of severe appendicitis in children, we divided our patients into 5 groups depending on the pathological findings (table (table1).1). Group 1 consisted of patients who had no pathological findings. Group 2 included patients who had reactive follicular hyperplasia or chronic appendicitis. Group 3 comprised patients who had acute appendicitis, intact appendiceal mucosa and a mild to moderate infiltrate of inflammatory cells. Group 4 consisted of patients who had macroscopically or histologically perforated acute appendicitis or perforated appendiceal mucosa that was accompanied by a strong panmural infiltrate of inflammatory cells. Group 5 consisted of patients who had acute necrotizing appendicitis. Histology at each study site was performed by a single pathologist in order to minimize intraobserver variation.

Table 1.
Description of the 5 groups to which patients were assigned based on severity of inflammation

Laboratory Parameters

The concentration of PCT from the German patients was determined using an enzyme-linked fluorescent assay (VIDAS® PCT; B.R.A.H.M.S. AG, Henningsdorf, Germany). The assay principle combined a 1-step immunoassay sandwich method with a final fluorescent detection performed in an automated VIDAS® instrument (bioMérieux SA, Marcy l’Etoile, France). The concentrations of PCT from the Spanish patients were determined using PCT-Q (B.R.A.H.M.S. AG), a self-developing, solid-phase immunoassay, according to the manufacturer's protocol. The leukocyte count of each patient was determined with an electronic cell counter (Sysmex XE-2100; Sysmex Digitana AG, Horgen, Switzerland). The serum CRP measurement combined an in vitro particle-enhanced immunoturbidimetric assay with a quantitative determination of CRP in human serum using an automated clinical chemistry analyzer (Roche® Modular Analytics P 800, Roche, Mannheim, Germany) in combination with a commercially available standardized test kit (Tina-quant CRP Latex; Roche Diagnostics GmbH, Mannheim, Germany). Normal values were defined as 4.4–11.3/nl for WBC, <0.5 mg/dl for CRP and <0.5 μg/ml for PCT.


Statistical analysis was performed using SPSS 11.0 for Windows. Analysis of the data distribution was assessed by the Kolmogorov-Smirnov test. For normally distributed data (WBC), the null hypothesis was based on the assumption that no difference in values existed between the 5 groups that were studied in the present trial. One-way ANOVA for 5 independent samples was used for interarm comparisons. The Scheffe procedure for pairwise comparisonswas subsequently used to determine which group was significantly different from any other in terms of WBC value.

In the case of unequal data distribution (CRP), the Kruskal-Wallis test (nonparametric independent-group comparison) was used to compare groups. The Mann-Whitney U Test was applied to compare different groups among themselves. The PCT values were analyzed by means of χ2 analysis. Mean values and standard deviations (SD) were calculated for WBC and CRP. Additional calculations of sensitivity, specificity, negative predictive value (NPV) and positive predictive value(PPV) of PCT, WBC and CRP for the diagnosis of appendicitis were included in the data analysis. Statistical significance was set at the 5% level (2-sided).


A total of 103 patients (46 female, 57 male, median age ± SD: 33 ± 18.5 years, range: 10–86) with clinical signs of acute appendicitis who received an appendectomy were enrolled in the study. After a review of the operation and the pathology record, the patients were classified into 1 of 5 groups (table (table1).1). Group 1 included a total of 5 patients (4.8%), group 2 comprised 14 patients (13.6%), group 3 consisted of 60 patients (58.3%), group 4 included 20 patients (19.4%) and group 5 comprised 4 patients (3.9%; fig. fig.11).

Fig. 1.
Percentage of patients with elevated PCT levels (>0.5 μg/ml) among groups 1–5.

Demographics and Treatment

Two patients from group 1 (40%), 5 patients from group 2 (36%), 17 patients from group 3 (28%) and 1 patient from group 4 (2%) were 18 years of age or younger. The prevalence of women was noticeably higher in group 2 (71%), whereas men were more often affected in group 4 (60%). In groups 1 and 5, the ratio of men to women was almost equal (table (table2).2). The surgical treatment was laparoscopic (n = 82), open (n = 16) or conversion appendectomy (n = 5). The mean duration of the hospital stay was 4.7 days (SD = 2.9, range = 2–21). The details are compiled in table table22.

Table 2.
Details of laboratory values, epidemiology and treatment of the 5 groups in the present study

Laboratory Results

The PCT values were elevated in 14 of the 98 patients (14.3%) with some form of appendicitis. Of those 14 patients, none (0%) were from group 1 (n = 5), 1 (7%) was from group 2 (n = 14), 4 (7%) were from group 3 (n = 60), 6 (33%) were from group 4 (n = 20) and 3 (75 %) were from group 5 (n = 4) (fig. (fig.1).1). Four patients had PCT values between 0.5 and 2 μg/ml, none had PCT values of 2–10 μg/ml, and 1 had PCT values >10 μg/ml. The sensitivity of PCT for the diagnosis of acute appendicitis was 0.14, with a specificity of 1. The PPV of PCT (>0.5 μg/ml) for appendicitis was 1 in contrast to 0.76 for CRP (>0.5 mg/dl) and to 1 for WBC. The NPV for appendicitis were 0.06 for PCT, 0.09 for WBC and 0.11 for CRP. Compared to group 1 (no appendicitis), the patients with appendicitis in the other groups (groups 2–5) had a significantly higher likelihood of having a PCT elevation (p < 0.05). Details of the sensitivity, specificity and predictive values are compiled in table table33.

Table 3.
Sensitivity, specificity, PPV and NPV for appendicitis calculated for PCT, WBC and CRP

The mean values for WBC were 13/nl (SD = 5.2, range = 3.4–31). Statistical analysis showed that there was a significant difference in the WBC values between groups 1 and 5 as well as between groups 2 and 5, whereas group 5 showed significantly higher values overall (p < 0.05). All other differences were not significant (p > 0.05) (fig. (fig.22).

Fig. 2.
WBC levels in groups 1–5. The WBC levels showed significantly higher values in group 5 when compared to groups 1 and 2 (p < 0.05).

The mean CRP value was 8.8 mg/dl (SD = 13, range = 0–60.2). Comparison among the groups showed significantly higher values in groups 4 and 5 compared to groups 1–3 (p < 0.05). Group 3 also showed significantly higher CRP values compared to group 2 (p < 0.05). All other differences among the groups were not significant (p > 0.05; fig. fig.33).

Fig. 3.
CRP levels in groups 1–5. Notice the significantly higher CRP values in groups 4 and 5 as compared to group 1, 2 and 3 (p < 0.05). Group 3 showed significantly higher CRP values compared to group 2 (p < 0.05).

Overall, with regard to WBC, CRP and PCT values, there was an increase in the mean values from group 2 to group 5, which reflected the severity of the appendicitis. The laboratory findings are compiled in table table22.


PCT is a calcitonin precursor and has emerged as one of the most important early laboratory signs for systemic bacterial and fungal infection. The group of calcitonin precursors, including PCT, are encoded by the calcitonin I gene (CALC-I) on chromosome 11 [5]. In the absence of inflammatory stimuli, the transcription of the CALC-I gene is suppressed, except in neuroendocrine cells that are mainly found in the thyroid or lung. In healthy individuals, the normal plasma PCT levels are <0.5 ng/ml. In cases of microbial infection, severe systemic inflammation or sepsis, an increase in CALC-I gene expression occurs, with a concomitant rise in PCT levels in all tissues and cell types of the human body [6]. The circulating levels of the calcitonin precursors, including the prohormone PCT, have been shown to be increased up to several thousand-fold, reflecting the severity of the inflammation. Not only is PCT elevated in systemic infections, but it is also increased for some localized bacterial infections, such as in lower respiratory tract infections, meningitis, infectious endocarditis and pancreatitis, and its important diagnostic performance has been demonstrated for these localized infections [7,8,9,10].

In the early disease process, acute appendicitis is also regarded as a localized inflammatory process. However, the diagnostic potential of PCT has not been sufficiently investigated for this particular disease.

From a pathophysiological point of view, fecaliths, which naturally carry a high load of gram-negative bacteria, are thought to be the predominant factor for the obstruction of the appendiceal lumen in acute appendicitis. Over the course of the inflammatory process, mucosal secretion increases and the resident bacteria of the appendix start to multiply rapidly. The integrity of the appendix is compromised through an impaired blood supply, and the bacterial invasion begins to proceed with the release of bacterial endotoxins [11]. At the same time, it has been demonstrated that the injection of bacterial endotoxin into healthy subjects causes an increase in PCT by approximately 0.5 ng/ml per hour after a latency of about 2–3 h and reaching a plateau after 6–12 h [12]. Bacterial lipopolysaccharides are unique surface glycolipids and prototypical examples of bacterial endotoxins, which are found in the outer membrane of gram-negative bacteria. Bacterial lipopolysaccharides and the proinflammatory cytokines are the most potent inducers of PCT release. During a viral infection, the serum PCT concentrations were slightly elevated to 1.5 ng/ml, whereas the levels during a bacterial infection reached up to 1,000 ng/ml [13]. Therefore, an increase in PCT in patients with acute appendicitis can be expected.

The available data evaluating the diagnostic value of PCT for patients with acute appendicitis are sparse and predominantly related to children. Acute appendicitis in adolescents or adults has rarely been subject of research regarding the diagnostic value of PCT. The only study available to date that investigated adult patients is by Kisacik et al. [14], who studied 34 adult patients with acute appendicitis and 28 with familial Mediterranean fever attacks. They report PCT levels that are higher than 0.5 ng/ml in 62% of their patients with acute appendicitis and in 11% of their patients with familial Mediterranean fever. When our data are compared with the latter findings, we see in their data a remarkably large fraction of patients with acute appendicitis who demonstrate a PCT increase, which is contradictory to our data. In our group of 98 patients with some form of appendicitis (severity 1–5), only 14 (14.3%) had PCT levels >0.5 ng/ml. The sensitivity of PCT for the diagnosis of appendicitis was calculated to be 0.14 in our study, which is remarkably low and causes some doubt about the diagnostic potential of this laboratory marker. The other available data include children and adolescents, and these 4 studies show conflicting results [4,15,16,17].

In contrast to the latter results, our patients showed that the likelihood of observing an elevation in PCT values increases with the severity of appendicitis. Groups 3, 4 and 5 showed a gradual increase in the proportion of patients with an elevation in the levels of PCT (7, 33 and 75%).

In the present study, only 14 of 98 patients (14.3%) with appendicitis showed an increase in PCT values, which leads us to conclude that PCT has no diagnostic value for acute appendicitis in adolescents or adults. This is also reflected by the poor sensitivity of 0.14, which, from the authors’ point of view, prohibits its usage as a potential diagnostic marker.

As our first results contradicted those reported by Kisacik et al. [14], very early on in our study, we began analyzing the method of determining the levels of PCT and specimen collection to rule out systematic errors that may have led to lower PCT concentrations than those reported by other authors. In addition, since the PCT controls, who were all septic patients from the surgical ICU of our respective departments, were positive and evaluated identically, we concluded that the measurement of PCT was without error.

In contrast, other inflammatory parameters, such as CRP and leukocytes, showed a correlation with the severity of appendiceal inflammation. This was expected, as had been shown before by other authors [18].

With regard to PCT, the question arises as to why acute appendicitis seems not to be among those localized bacterial infections that result in a parallel increase in this inflammatory marker. First of all, uncomplicated acute appendicitis does not cause significant bacterial translocation. This has been shown in a study by Aslan et al. [19], where only 3 of 18 patients (16.6%) with acute appendicitis also included bacterial translocation to a mesenteric lymph node that was adjacent to the terminal ileum. We propose that a lipopolysaccharide-induced increase in PCT occurs mainly in cases of significant systemic bacterial translocation. This could explain the reason why the PCT response in our collective was so low. This is consistent with our results, in which patients from groups 3–5 experienced an elevation in the level of PCT.

Finally, although the appendix is the most commonly resected and examined intra-abdominal organ, the pathogenesis and etiology of acute appendicitis remains enigmatic. Reports of a viral origin of appendiceal inflammation may lend support to the theory that a viral infection may contribute in part to some cases of appendicitis and thus be relevant to the low PCT levels observed [20, 21].


As to whether or not PCT should be a marker of appendicitis in adult patients, the answer is clearly no. It is potentially increased in cases of severe inflammation and, in particular, after appendiceal perforation or gangrenous appendicitis, which confirms its broad usage as a sepsis marker. However, its frequency of elevation with a remarkably poor sensitivity of 0.14 prohibits its use as a standard laboratory test for the diagnosis of acute or chronic appendicitis.

Financial Disclosure

All authors hereby disclose any commercial associations which might pose or create a conflict of interest with information presented in the manuscript.


This study was conducted in compliance with the applicable local requirements with regard to the ethics and investigational committee review, informed consent, and other statutes or regulations regarding the protection of the rights and welfare of human subjects participating in medical research (ClinicalTrials.gov identifier: NCT00630071, Ethics Review Board of the Ruhr University Bochum, Germany, registration No.: 3133-08).


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