• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Apr 1963; 85(4): 842–850.
PMCID: PMC278235

HEMIN BIOSYNTHESIS IN HAEMOPHILUS

Abstract

White, David C. (The Rockefeller Institute, New York, N.Y.) and S. Granick. Hemin biosynthesis in Haemophilus. J. Bacteriol. 85:842–850. 1963.—Hemin-independent Haemophilus species have been shown to form hemin by the classical hemin biosynthetic pathway. Three distinct species of Haemophilus [H. influenzae, H. aegyptius, and H. canis (H. haemoglobinophilus)] all lost the enzymatic capacities to convert δ-aminolevulinic acid to protoporphyrin, which accounts for their dependence on hemin for growth. The strain of H. aegyptus tested cannot form hemin from protoporphyrin, can be transformed with deoxyribonucleic acid (DNA) from H. influenzae, and the resultant progeny have the enzymatic activity to convert protoporphyrin to hemin. Attempts to transform these species to hemin independence with DNA from hemin-independent H. parainfluenzae are unsuccessful under conditions where streptomycin resistance is readily transformed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BARRETT J. The prosthetic group of cytochrome a2. Biochem J. 1956 Dec;64(4):626–639. [PMC free article] [PubMed]
  • BOGORAD L. The enzymatic synthesis of porphyrins from porphobilinogen. I. Uroporphyrin I. J Biol Chem. 1958 Aug;233(2):501–509. [PubMed]
  • BOGORAD L. The enzymatic synthesis of porphyrins from porphobilinogen. II. Uroporphyrin III. J Biol Chem. 1958 Aug;233(2):510–515. [PubMed]
  • BRUMFITT W. Some growth requirements of Haemophilus influenzas and Haemophilus pertussis. J Pathol Bacteriol. 1959 Jan;77(1):95–100. [PubMed]
  • ELLIOTT WH. Aminoacetone formation by Staphylococcus aureus. Biochem J. 1960 Mar;74:478–485. [PMC free article] [PubMed]
  • KIKUCHI G, KUMAR A, TALMAGE P, SHEMIN D. The enzymatic synthesis of delta-aminolevulinic acid. J Biol Chem. 1958 Nov;233(5):1214–1219. [PubMed]
  • LABBE RF, HUBBARD N. Preparation and properties of the iron-protoporphyrin chelating enzyme. Biochim Biophys Acta. 1960 Jul 1;41:185–191. [PubMed]
  • LASCELLES J. Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis. Biochem J. 1959 Jul;72:508–518. [PMC free article] [PubMed]
  • LASCELLES J. The synthesis of enzymes concerned in bacteriochlorophyll formation in growing cultures of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Dec;23:487–498. [PubMed]
  • LASCELLES J. Synthesis of tetrapyrroles by microorganisms. Physiol Rev. 1961 Apr;41:417–441. [PubMed]
  • ALEXANDER HE, HAHN E, LEIDY G. On the specificity of the desoxyribonucleic acid which induces streptomycin resistance in Hemophilus. J Exp Med. 1956 Sep 1;104(3):305–320. [PMC free article] [PubMed]
  • LEIDY G, HAHN E, ALEXANDER HE. Interspecific transformation in Hemophilus: a possible index of relationship between H. influenzae and H. aegyptius. Proc Soc Exp Biol Med. 1959 Oct;102:86–88. [PubMed]
  • LEIDY G, JAFFEE I, ALEXANDER HE. Emergence of competence (for transformation) of three Hemophilus species in a chemically defined environment. Proc Soc Exp Biol Med. 1962 Dec;111:725–731. [PubMed]
  • MAUZERALL D, GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed]
  • MOSES V. Tricarboxylic acid cycle reactions in the fungus Zygorrhynchus moelleri. J Gen Microbiol. 1955 Oct;13(2):235–251. [PubMed]
  • NICHOLAS REH, RIMINGTON C. Paper chromatography of porphyrins; some hitherto unrecognized porphyrins and further notes on the method. Biochem J. 1951 Mar;48(3):306–309. [PMC free article] [PubMed]
  • SANO S, GRANICK S. Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation. J Biol Chem. 1961 Apr;236:1173–1180. [PubMed]
  • SCHAEFFER P. Transformation interspécifique chez des bactéries du genre Hemophilus. Ann Inst Pasteur (Paris) 1956 Aug;91(2):192–211. [PubMed]
  • SCHAEFFER P. Interspecific reactions in bacterial transformation. Symp Soc Exp Biol. 1958;12:60–74. [PubMed]
  • WHITE DC, SMITH L. Hematin enzymes of Hemophilus parainfluenzae. J Biol Chem. 1962 Apr;237:1332–1336. [PubMed]
  • WHITE DC. Cytochrome and catalase patterns during growth of Haemophilus parainfluenzae. J Bacteriol. 1962 Apr;83:851–859. [PMC free article] [PubMed]
  • WHITE DC. Respiratory systems in the hemin-requiring Haemophilus species. J Bacteriol. 1963 Jan;85:84–96. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...