• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. May 1965; 89(5): 1169–1175.
PMCID: PMC277623

Vitamin Requirements of Several Cellulolytic Rumen Bacteria1


Scott, H. W. (Ohio Agricultural Experiment Station, Wooster), and B. A. Dehority. Vitamin requirements of several cellulolytic rumen bacteria. J. Bacteriol. 89:1169–1175. 1965.—Four strains of cellulolytic bacteria recently isolated from in vitro rumen fermentations were used in this study. Nine water-soluble vitamins were tested in single-deletion and single-addition plus biotin experiments, each with and without charcoal-extracted casein hydrolysate. Bacteroides succinogenes A3C and B21a required only biotin under the above experimental conditions. Ruminococcus flavefaciens B34b showed an absolute requirement for biotin and was stimulated by p-aminobenzoic acid (PABA) in the single-deletion experiments. In the single-addition plus biotin experiments, PABA and, to a lesser extent, vitamin B12 appeared to be required for maximal growth. The presence or absence of casein hydrolysate did not affect the vitamin requirements for the aforementioned three strains. In the single-deletion experiments, R. flavefaciens Cla showed an absolute requirement for biotin and, when casein hydrolysate was omitted, for B12. When casein hydrolysate was present, no requirement for B12 could be observed. In the single-addition experiments where the basal medium contained biotin and casein hydrolysate or B12, PABA was required for maximal growth; however, the single deletion of PABA caused only slight retardation of growth. Investigation of the B12 or casein hydrolysate requirement of Cla revealed that a mixture of purified amino acids simulating casein hydrolysate satisfied this requirement. Subsequent work indicated that this requirement could be satisfied by the amino acid methionine.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1023K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BENTLEY OG, JOHNSON RR, HERSHBERGER TV, CLINE JH, MOXON AL. Cellulolytic-factor activity of certain short-chain fatty acids for rumen microorganisms in vitro. J Nutr. 1955 Nov 10;57(3):389–400. [PubMed]
  • Bryant MP, Robinson IM. Some Nutritional Requirements of the Genus Ruminococcus. Appl Microbiol. 1961 Mar;9(2):91–95. [PMC free article] [PubMed]
  • BRYANT MP, ROBINSON IM. Some nutritional characteristics of predominant culturable ruminal bacteria. J Bacteriol. 1962 Oct;84:605–614. [PMC free article] [PubMed]
  • DRYDEN LP, HARTMAN AM, BRYANT MP, ROBINSON IM, MOORE LA. Production of vitamin B12 and vitamin B12 analogues by pure cultures of ruminal bacteria. Nature. 1962 Jul 14;195:201–202. [PubMed]
  • GIBSON F, WOODS DD. The synthesis of methionine by suspensions of Escherichia coli. Biochem J. 1960 Jan;74:160–172. [PMC free article] [PubMed]
  • GILL JW, KING KW. Nutritional characteristics of a Butyrivibrio. J Bacteriol. 1958 Jun;75(6):666–673. [PMC free article] [PubMed]
  • GUEST JR, HELLEINER CW, CROSS MJ, WOODS DD. Cobalamin and the synthesis of methionine by ultrasonic extracts of Escherichia coli. Biochem J. 1960 Aug;76:396–405. [PMC free article] [PubMed]
  • HUNGATE RE. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. [PMC free article] [PubMed]
  • JONES KM, GUEST JR, WOODS DD. Folic acid and the synthesis of methionine by extracts of Escherichia coli. Biochem J. 1961 Jun;79:566–574. [PMC free article] [PubMed]
  • KISLIUK RL, WOODS DD. Interrelationships between folic acid and cobalamin in the synthesis of methionine by extracts of Escherichia coli. Biochem J. 1960 Jun;75:467–477. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...