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Commun Integr Biol. 2009 Sep-Oct; 2(5): 448–451.
PMCID: PMC2775248

Neurospora sees the light

Light signaling components in a model system

Abstract

Light is a key environmental signal for most life on earth. Over 5% of Neurospora crassa genes are expressed in response to light stimulation in a temporally regulated cascade that includes several transcription factors. Fungal genomes, including Neurospora's, may encode several different proteins capable of binding chromophores with the ability to harvest light energy as well as proteins that can interact with primary photoreceptors or further propogate the light signal. The best understood photo- receptors are the evolutionarily conserved White Collar proteins, and the related Vivid protein, but fungi may also encode phytochromes, cryptochromes and opsins.

Key words: light, microarray, Neurospora, photoreceptors, wc-1, wc-2, vvd, frq, sub-1, nop-1, ve-1

According to a recent report from the American Society of Microbiology, the fungal kingdom comprises an estimated 1.5 million species, many hundreds of which are known animal or plant pathogens.1 The filamentous fungus Neurospora crassa is a leading research model, including studies aimed at understanding light responses in fungal cells.27 Decades of effort from several labs, have established the White Collar complex (WCC) as an essential as well as dominant light signaling component. The heterodimeric transcription factor WCC senses light directly through bound FAD, and binds to the promoters of many light-responsive genes, activating gene expression. We have shown that light regulated expression falls into two distinct temporal classes, both under WCC control. SUB-1, identified as an “early” light-responsive transcription factor, was found to regulate most of the “late” light gene expression. Chromatin-immunoprecipitation (ChIP) and bioinformatics analysis further established the hierarchical relationship between early and late light responses.8 Here, we present a brief summary of recent studies on the molecular components involved in Neurospora photobiology.

Light-Regulated Biology in Neurospora crassa

Light acts as an essential cue to regulate a variety of physiological processes in Neurospora, including the resetting of the circadian clock, biosynthesis of the photo-protective carotenoid pigments, asexual conidiospore formation, perithecial development in the sexual cycle, and the direction of ascospores release.27 Underlying this biology is the regulation of many Neurospora genes by light. Microarrays representing the approximately 10,000 genes in Neurospora crassa were used as probes against light induced cDNA. Of the 5,600 detectable genes, 314 (approximately 5.6%) responded to the light stimulus by increasing transcript levels.8 Most of the identified genes (92%) were either early (45%), with peak expression between 15 and 45 minutes, or late (55%), with the induced expression peaking between 45 and 90 minutes after lights on. Genes related to the synthesis of photoprotective pigments (7.1%), vitamins, cofactors, and prosthetic groups (4.7%), secondary metabolism (4.7%), DNA processing (6.3%), cellular signaling (5.5%) and environmental sensing and response (1.6%) were found enriched in the early light response. In contrast, genes involved in carbohydrate metabolism (20%), oxidation of fatty acids (1.9%) and oxygen detoxification reaction (2.5%) were found enriched in the late light response. Within the early group were several transcription factors, most of which show mutant phenotypes during development (see Table 1).

Table 1
Real and putative light signaling components in Neurospora crassa

All currently known light responses in Neurospora are restricted to near UV/blue light,5,7 suggesting the presence of a master photoreceptor dedicated to blue light sensing and signal transduction. Extensive genetic screening and analysis has resulted in the isolation of only two fully blind mutants, wc-1 and wc-2,3,9,10 both GATA family zinc finger transcription factors.11 The direct connection between light sensing and gene activation has subsequently been demonstrated both in vitro and in vivo.1215 The photoreceptor WC-1 forms an obligate complex with WC-2 to bind to specific DNA sequences,16,17 including the promoter of a light-responsive transcription factor, sub-1, the function of which is essential for the late light response.8 Several additional components are or may be involved in the light signaling mechanism (Fig. 1). As the WCC has been reviewed in some detail elsewhere,27 our discussion below will focus on the additional players.

Figure 1
Established and putative molecular components involved in Neurospora light signaling. WC-1 and WC-2 form a heterodimeric transcription factor (WCC) that binds to early light responsive elements (ELREs). In response to a light signal, transcription is ...

Real and Putative Light Signaling Components in Neurospora crassa

Real and putative light signaling components in Neurospora are summarized in Table 1. After the WCC, VVD has been the next most intensely studied photoreceptor in the fungi. Our study and others have clearly shown that VVD acts as a universal repressor for most if not all light-induced gene expression controlled by the WCC.8,1821 In vvd mutants, once gene transcription is turned on by the light-activated WCC, transcript levels will remain upregulated for many hours in constant light, so-called “photoadaptation defects”. In contrast, in a wild-type strain, light-induced gene expression is transient, usually returning to pre-induction levels within two to four hours.8 Molecularly, VVD is a small, 21 kD flavin-binding photoreceptor consisting of a LOV (light, oxygen or voltage) domain and N-terminal cap.22 Upon light activation, the formation of a protein-flavin bond in the LOV domain induces a conformational change at the N-terminus which appears to be essential for the light function of VVD.22 Formation of a rapidly exchanging VVD dimer in light has been proposed recently.23 Interestingly, VVD appears to localize exclusively in the cytosol while the majority of WCC is in the nucleus.19,24 This raises the question of how VVD communicates with the WCC to repress light responses and regulate various circadian clock properties.21,25,26 The answer to this question will certainly shed light on the molecular mechanisms of photoadaptation in general.

After completion of the Neurospora genome project,27 two putative red-light photoreceptors (N. crassa phytochrome orthologs phy-1 and phy-2) and one additional blue-light photoreceptor (N. crassa cryptochrome orthologue cry) were identified. Although there is yet no report of red light-regulated biology in Neurospora, a collaborative effort has shown that PHY-2 can covalently bind either biliverdin or phycocyanobilin and is capable of undergoing a photocycle in vitro.28 The cry gene encodes a member of the cryptochrome-DASH family. We have found it capable of binding FAD and MTHF, with both transcript and protein levels strongly induced by blue light in a wc-1 dependent manner.29 However, due to the lack of a detectable phenotype or atypical light responses in the respective knockout strains,8,28,29 the biological function(s) of PHY-1, PHY-2 and CRY remains to be discovered in Neurospora, although function has been reported for homologs in other fungi.3,4,6,30 The opsin, NOP-1, is a putative green-light photoreceptor identified via sequence homology with archaeal rhodopsins.31 NOP-1 has been shown to both bind retinal and undergo a slow photocycle32 and the expression levels of several genes are known to be affected in a knockout strain during late asexual development.33 Our microarray data, not carried past two hours after light stimulus in the knock-out strain, suggest that NOP-1 does not play a role in either early or late light regulated gene induction.8

Instead of sensing light directly, which requires the ability to interact with chromophores that absorb light energy, the FRQ and SUB-1 proteins are indirectly involved in light signaling. Our array analysis has confirmed and extended the role of FRQ in regulating the light function of the WCC by affecting the amplitude of induction for both types of light responses.8 Given that FRQ is an oscillating circadian clock component and has been shown to physically interact with the WCC, previous studies34,35 together with our recent microarray data highlight the clock-modulating effect of the light input pathway. The novel GATA family transcription factor, SUB-1, was identified as essential for regulating a subset of the early and most of the late light responses.8 A previously described phenotype associated with the sub-1 (submerged protoperithecia-1) knockout strain36 might easily be the functional consequence of impaired late light responses (i.e., the formation of protoperithecia in Neurospora is a light-regulated developmental process), which might also hold true for developmental defects seen in knockout strains of other light-responsive transcription factors.36,37

Finally, the veA locus has been shown to be required for both light-regulated development and secondary metabolism in Aspergillus nidulans38,39 and the promoter and coding sequences of the N. crassa ortholog, ve-1, is sufficient to complement the role of veA null mutants in A. nidulans.40 However, unlike its counterpart, VE-1 knockout strains in Neurospora lack light-dependent phenotypes40 and have largely normal gene expression in response to white light (Chen C-H and Loros J, unpublished data) suggesting that ve-1 may not have a significant role in regulating light signals in N. crassa, at least under the conditions tested.

Fungal Light Signaling Components are Conserved

Sequence and functional orthologs of WC-1, WC-2 and most of the other light signaling components are widespread among the fungal kingdom. Recent studies have demonstrated that WC-1- and WC-2-like molecules in various fungal species play an essential role in mediating light signals from the Ascomycota, Basidiomycota and Zygomycota phyla.24,6,7 Of broader evolutionary interest, WC-1 and the animal circadian-clock-associated bHLH transcription factors, CYC from insects and BMAL1 and NPAS2 from mammals, share a common ancestor. The bHLH transcription factors do not bind chromophores but, like WC-1 in Neurospora, they are critical for light resetting, as well as the maintenance of circadian rhythms in animals, highlighting the close evolutionary relationship between photobiology and circadian rhythmicity.41,42 Successful work on the WCC in Neurospora has led to fundamental breakthroughs in understanding photobiology in other fungi. We predict that future work on the underlying mechanisms of Neurospora light signaling components will continue to illuminate other light-sensitive eukaryotic cells.

Acknowledgements

This work was supported by grants from the National Institutes of Health to J.J.L. (RO1 GM08336), to Jay C. Dunlap and J.J.L. (PO1GM68087), and by the core grant to the Norris Cotton Cancer Center at Dartmouth. We thank Randy Lambreghts for critical reading of the manuscript and are deeply grateful to the Fungal Genetics Stock Center at the University of Missouri, Kansas City in supporting our work with Neurospora.

Footnotes

Previously published online as a Communicative & Integrative Biology E-publication: http://www.landesbioscience.com/journals/cib/article/8835

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