• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Aug 1964; 88(2): 293–296.
PMCID: PMC277298

CALCIUM ION REQUIREMENT FOR PROLIFERATION OF BACTERIOPHAGE [var phi]μ-4

Abstract

Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Calcium ion requirement for proliferation of bacteriophage [var phi]μ-4. J. Bacteriol. 88:293–296. 1964.—Divalent ions are essential for proliferation of phage [var phi]μ-4. Small amounts of citrate interfere with efficient adsorption of phage to the host cells. Penetration of phage material into the cell is strictly dependent on divalent ions and is inhibited by low levels of citrate. Inhibition of infection can be partially reversed, in early latent period, by calcium ions. Synthesis of new phage particles is also dependent on divalent ions. Addition of citrate to infected cell suspensions significantly reduced the number of phage progeny produced. Chelates such as phosphate and citrate rapidly inactivated the free phage particles at 65 C. Chelate inactivation of phage is not reversible; however, it can be prevented to some degree by calcium ions.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (561K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BARDSDALE WL, PAPPENHEIMER AM., Jr Phage-host relationships in nontoxigenic and toxigenic diphtheria bacilli. J Bacteriol. 1954 Feb;67(2):220–232. [PMC free article] [PubMed]
  • Delbrück M. Biochemical Mutants of Bacterial Viruses. J Bacteriol. 1948 Jul;56(1):1–16. [PMC free article] [PubMed]
  • GAREN A, PUCK TT. The first two steps of the invasion of host cells by bacterial viruses. II. J Exp Med. 1951 Sep;94(3):177–189. [PMC free article] [PubMed]
  • KAY D. The effect of divalent metals on the multiplication of coli bacteriophage T 5st. Br J Exp Pathol. 1952 Jun;33(3):228–235. [PMC free article] [PubMed]
  • LANNI YT. Invasion by bacteriophage T5. II. Dissociation of calcium-independent and calcium-dependent processes. Virology. 1960 Apr;10:514–529. [PubMed]
  • LARK KG, ADAMS MH. The stability of phages as a function of the ionic environment. Cold Spring Harb Symp Quant Biol. 1953;18:171–183. [PubMed]
  • LURIA SE, STEINER DL. The role of calcium in the penetration of bacteriophage T5 into its host. J Bacteriol. 1954 Jun;67(6):635–639. [PMC free article] [PubMed]
  • PERLMAN D, LANGLYKKE AF, ROTHBERG HD., Jr Observations on the chemical inhibition of Streptomyces griseus bacteriophage multiplication. J Bacteriol. 1951 Feb;61(2):135–143. [PMC free article] [PubMed]
  • POTTER NN, NELSON FE. Role of calcium and related ions in proliferation of lactic streptococcus bacteriophage. J Bacteriol. 1953 Nov;66(5):508–516. [PMC free article] [PubMed]
  • ROUNTREE PM. The role of certain electrolytes in the adsorption of staphylococcal bacteriophages. J Gen Microbiol. 1951 Oct;5(4):673–680. [PubMed]
  • Shafia F, Thompson TL. Isolation and preliminary characterization of bacteriophage phi-mu-4. J Bacteriol. 1964 May;87(5):999–1002. [PMC free article] [PubMed]
  • THOMPSON TL, SHAFIA F. Energy requirement for adsorption of bacteriophage phi-mu-4. Biochem Biophys Res Commun. 1962;8:467–470. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...