• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Genet. Author manuscript; available in PMC Oct 26, 2009.
Published in final edited form as:
PMCID: PMC2766813
NIHMSID: NIHMS132063

Evaluating signatures of sex-specific processes in the human genome

Abstract

Comparing levels of genetic variation between the X chromosome and autosomes can reveal the different demographic histories of males and females of a species. Taking this approach, two new studies report that the effective population sizes of men and women differ, but they disagree as to which sex outnumbered the other.

Evolutionary forces and social practices that differentially impact males and females can lead to divergent patterns of genetic variation among the autosomes, X chromosome, Y chromosome and the mitochondrial genome1,2. For example, populations in which a few males father a disproportionate number of offspring show low levels of Y-chromosomal genetic diversity. Likewise, societies in which females migrate to their husbands’ homes after marriage (patrilocality) experience more gene flow of X chromosomes and mtDNA3,4. Investigating genomic signatures of sex-biased processes has relied on comparing the nonrecombining Y chromosomes and mitochondrial genomes5, and it is hoped that the availability of genome-level data will provide a much finer-scale view of both patterns and processes68. On page 66 of the current issue, David Reich and colleagues9 report evidence for accelerated genetic drift on the X chromosome (that is, smaller effective population size, Ne, of females) during human migrations out of Africa, based on finding disproportionately low sequence variation and high SNP frequency differentiation of the X chromosome relative to that of the autosomes in non-African populations. Their results are in contrast to a recent paper by Michael Hammer and colleagues, who with a different study design estimated an unexpectedly high X-chromosomal diversity10.

More men on the move?

Keinan et al.9 compared genetic diversity among 130,000 X-chromosomal and autosomal SNPs genotyped in individuals of Northern European, West African and East Asian ancestry11,12. They use a statistic Q that compares rates of genetic drift of X-linked and autosomal SNPs and has an expected value of 0.75 when the effective population size of males and females is equal. Comparing the Northern European and East Asian samples, they find that Q is statistically indistinguishable from the expectation of 0.75 (ref. 9). However, comparing either non-African population with the West African samples, Q drops significantly below 0.75, which suggests faster genetic drift for the X chromosome in populations outside of Africa. Keinan et al.9 also compared large amounts of sequence data found in public databases for five individuals of Northern European ancestry, four of East Asian ancestry and five of West African ancestry. They find ratios of pairwise sequence diversity close to 0.75 in the West African population and below 0.75 outside of Africa (Fig. 1), which supports their SNP analysis. They demonstrate with simulations that demographic models with a smaller effective number of mating females in non-African populations are necessary to explain their results. Some sex-biased forces that could produce this pattern include recurrent long-range male migration from African sources or a shorter generation time for females.

Figure 1
Contrasting patterns of X-chromosomal and autosomal diversity. Data are from Keinan et al.9 and Hammer et al.10. (a) Pairwise sequence diversity values for the X chromosome (πX) and autosomes (πaut). (b) Ratios of X-chromosomal to autosomal ...

But the story may not be so clear. In a study with a similar goal of comparing X-chromosomal and autosomal genetic variation, Michael Hammer and colleagues10 analyzed over 210 kilobases of DNA sequence data from across 20 independent regions on the X chromosome and 20 independent regions on the autosomes, sequenced in each of 90 individuals from six geographically diverse populations (Fig. 1a)10. They estimate the ratio of effective population size on the X chromosome (NX) and autosomes (NA) from two summary statistics of the data—the observed divergence between human and orangutan (used to account for variation in mutation rate among chromosomes) and the number of segregating sites in the sample (that is, SNPs in the data). They observe NX/NA is above 0.75 for all six surveyed populations, and in three cases it is significantly higher than 0.75 with no systematic out-of-Africa effect. They therefore conclude that the effective population size of females is larger than that of males. They also use simulations to demonstrate that sex-biased processes are needed to explain their observation, and they suggest that larger variance in male reproductive success relative to females (such as would occur under polygyny) may explain the data.

Finding common ground

In order to reconcile these findings, we compared a common summary statistic, π (pairwise nucleotide diversity), of the data reported by the two groups (Fig. 1). While the estimated autosomal nucleotide diversity (πaut) is very similar for both studies (≈0.12% per site or 1 SNP per 900 base pairs in African populations, and ≈0.08% or 1 SNP per 1,250 base pairs in European and East Asian populations), the estimated X-chromosomal nucleotide diversity (πX) differs substantially (Fig. 1a). Keinan et al.9 report approximately 0.72, 0.46 and 0.41 SNPs per kilobase of X chromosome in West African, North European and East Asian populations, respectively, whereas Hammer et al.10 find about 40–50% more nucleotide variation in each of their samples for the closest comparable populations (0.99, 0.71 and 0.58 for Mandenka, Han Chinese and Basque, respectively). Both studies effectively use πXaut to estimate Ne for males and females, so the higher overall levels of X-chromosomal diversity in Hammer et al.10 explain part of the discrepancy. It appears that the rest of the discrepancy is explained by different normalizations for background mutation rate differences between the X chromosome and autosomes (Hammer et al.10 used human-orangutan divergence and Keinan et al.9 used human-macaque divergence) (Fig. 1b).

While we are able to identify that the papers differ in their estimates of X-chromosomal genetic diversity and X-to-autosome mutational bias, how and why these differences arise remain open questions. Part of the answer may lie in the advantages and limitations of each study design. Hammer et al.10 studied population-level processes with direct resequencing among a relatively large number of individuals (which we advocate), but they investigated a limited number of genomic regions, selected to be far from genes and with high recombination rates to minimize possible confounding effects of natural selection. Keinan et al.9 used two different sources of genome-wide data, starting with SNP data, which is inherently more difficult to interpret due to unknown ascertainment biases in the data, and we commend their attempt to control for many such biases. They demonstrate further support for the observed signature of faster genetic drift on the X chromosome by comparing nucleotide diversity among pairs of chromosomes from each population. This approach, however, is less powerful than comparing the full spectrum SNP frequencies one observes in deep resequence data. Encouragingly, the genetic diversity estimates reported by Keinan et al.9 are nearly identical to those recently reported by Bentley et al.13 for a pair of fully sequenced X chromosomes of a Northern European female (0.47%).

In order to address continuing questions on the nature of sex-biased processes, full genome sequencing of large numbers of individuals sampled from diverse populations will be needed. The upcoming 1,000 Genomes Project (http://www.1000genomes.org/), for example, will provide orders of magnitude more data for these types of analyses. We share the enthusiasm of the population genetics community that this will bring the potential for resolving continuing questions regarding how human history and cultural practices have shaped global patterns of genomic diversity.

Contributor Information

Carlos D. Bustamante, Department of Biological Statistics and Computational Biology, Cornell University, 102 Weill Hall, Ithaca, New York 14850, USA.

Sohini Ramachandran, Society of Fellows and Department of Organismic and Evolutionary Biology, Harvard University, 4100 Biological Laboratories, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA. e-mail: ude.llenroc@82bdc.

References

1. Wilkins JF. Curr Opin Genet Dev. 2006;16:611–617. [PubMed]
2. Schaffner SF. Nat Rev Genet. 2004;5:43–51. [PubMed]
3. Seielstad MT, Minch E, Cavalli-Sforza LL. Nat Genet. 1998;20:278–280. [PubMed]
4. Wilder JA, Kingan SB, Mobasher Z, Pilkington MM, Hammer MF. Nat Genet. 2004;36:1122–1125. [PubMed]
5. Ségurel L, et al. PLoS Genet. 2008;4:e1000200. [PMC free article] [PubMed]
6. Oota H, Settheetham-Ishida W, Tiwawech D, Ishida T, Stoneking M. Nat Genet. 2001;29:20–21. [PubMed]
7. Wilkins JF, Marlowe FW. Bioessays. 2006;28:290–300. [PubMed]
8. Ramachandran S, Rosenberg NA, Feldman MW, Wakeley J. Theor Popul Biol. 2008;74:291–301. [PMC free article] [PubMed]
9. Keinan A, Mullikin JC, Patterson N, Reich D. Nat Genet. 2009;41:66–70. [PMC free article] [PubMed]
10. Hammer MF, Mendez FL, Cox MP, Woerner AE, Wall JD. PLoS Genet. 2008;4:e1000202. [PMC free article] [PubMed]
11. International HapMap Consortium. Nature. 2005;437:1299–1320. [PMC free article] [PubMed]
12. International HapMap Consortium. Nature. 2007;449:851–861. [PMC free article] [PubMed]
13. Bentley DR, et al. Nature. 2008;456:53–59. [PMC free article] [PubMed]
PubReader format: click here to try

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...