• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of mbcLink to Publisher's site
Mol Biol Cell. Oct 1996; 7(10): 1511–1519.
PMCID: PMC276002

Ca(2+)/calmodulin-dependent kinase is essential for both growth and nuclear division in Aspergillus nidulans.

Abstract

The calmodulin gene has been shown to be essential for cell cycle progression in a number of eukaryotic organisms. In vertebrates and Aspergillus nidulans the calmodulin dependence also requires calcium. We demonstrate that the unique gene encoding a multifunctional calcium/calmodulin-dependent protein kinase (CaMK) is also essential in A. nidulans. This enzyme is required both for the nuclear division cycle and for hyphal growth, because spores containing the disrupted gene arrest with a single nucleus and fail to extend a germ tube. A strain conditional for the expression of CaMK was created. When grown under conditions that resulted in a 90% decrease in the enzyme, both nuclear division and growth were markedly slowed. The CaMK seems to be important for progression from G2 to mitosis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baitinger C, Alderton J, Poenie M, Schulman H, Steinhardt RA. Multifunctional Ca2+/calmodulin-dependent protein kinase is necessary for nuclear envelope breakdown. J Cell Biol. 1990 Nov;111(5 Pt 1):1763–1773. [PMC free article] [PubMed]
  • Bartelt DC, Fidel S, Farber LH, Wolff DJ, Hammell RL. Calmodulin-dependent multifunctional protein kinase in Aspergillus nidulans. Proc Natl Acad Sci U S A. 1988 May;85(10):3279–3283. [PMC free article] [PubMed]
  • Bergen LG, Morris NR. Kinetics of the nuclear division cycle of Aspergillus nidulans. J Bacteriol. 1983 Oct;156(1):155–160. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Cherry JR, Johnson TR, Dollard C, Shuster JR, Denis CL. Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1. Cell. 1989 Feb 10;56(3):409–419. [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Harris SD, Morrell JL, Hamer JE. Identification and characterization of Aspergillus nidulans mutants defective in cytokinesis. Genetics. 1994 Feb;136(2):517–532. [PMC free article] [PubMed]
  • Kornstein LB, Gaiso ML, Hammell RL, Bartelt DC. Cloning and sequence determination of a cDNA encoding Aspergillus nidulans calmodulin-dependent multifunctional protein kinase. Gene. 1992 Apr 1;113(1):75–82. [PubMed]
  • Kulmburg P, Judewicz N, Mathieu M, Lenouvel F, Sequeval D, Felenbok B. Specific binding sites for the activator protein, ALCR, in the alcA promoter of the ethanol regulon of Aspergillus nidulans. J Biol Chem. 1992 Oct 15;267(29):21146–21153. [PubMed]
  • Lorca T, Cruzalegui FH, Fesquet D, Cavadore JC, Méry J, Means A, Dorée M. Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs. Nature. 1993 Nov 18;366(6452):270–273. [PubMed]
  • Ethical guidelines for publications of research. The Council of the Endocrine Society. Endocr Rev. 1993 Feb;14(1):1–2. [PubMed]
  • Lu KP, Rasmussen CD, May GS, Means AR. Cooperative regulation of cell proliferation by calcium and calmodulin in Aspergillus nidulans. Mol Endocrinol. 1992 Mar;6(3):365–374. [PubMed]
  • May GS. The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable. J Cell Biol. 1989 Nov;109(5):2267–2274. [PMC free article] [PubMed]
  • Mathieu M, Felenbok B. The Aspergillus nidulans CREA protein mediates glucose repression of the ethanol regulon at various levels through competition with the ALCR-specific transactivator. EMBO J. 1994 Sep 1;13(17):4022–4027. [PMC free article] [PubMed]
  • McGoldrick CA, Gruver C, May GS. myoA of Aspergillus nidulans encodes an essential myosin I required for secretion and polarized growth. J Cell Biol. 1995 Feb;128(4):577–587. [PMC free article] [PubMed]
  • Miller BL, Miller KY, Timberlake WE. Direct and indirect gene replacements in Aspergillus nidulans. Mol Cell Biol. 1985 Jul;5(7):1714–1721. [PMC free article] [PubMed]
  • Olsen DB, Eckstein F. High-efficiency oligonucleotide-directed plasmid mutagenesis. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1451–1455. [PMC free article] [PubMed]
  • Osmani SA, Pu RT, Morris NR. Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase. Cell. 1988 Apr 22;53(2):237–244. [PubMed]
  • Payne ME, Fong YL, Ono T, Colbran RJ, Kemp BE, Soderling TR, Means AR. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190–7195. [PubMed]
  • Planas-Silva MD, Means AR. Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2. EMBO J. 1992 Feb;11(2):507–517. [PMC free article] [PubMed]
  • Rasmussen C, Garen C, Brining S, Kincaid RL, Means RL, Means AR. The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans. EMBO J. 1994 Jun 1;13(11):2545–2552. [PMC free article] [PubMed]
  • Rasmussen CD, Means RL, Lu KP, May GS, Means AR. Characterization and expression of the unique calmodulin gene of Aspergillus nidulans. J Biol Chem. 1990 Aug 15;265(23):13767–13775. [PubMed]
  • Rasmussen C, Rasmussen G. Inhibition of G2/M progression in Schizosaccharomyces pombe by a mutant calmodulin kinase II with constitutive activity. Mol Biol Cell. 1994 Jul;5(7):785–795. [PMC free article] [PubMed]
  • Rasmussen G, Rasmussen C. Calmodulin-dependent protein kinase II is required for G1/S progression in HeLa cells. Biochem Cell Biol. 1995 Mar-Apr;73(3-4):201–207. [PubMed]
  • Roskoski R., Jr Assays of protein kinase. Methods Enzymol. 1983;99:3–6. [PubMed]
  • Soderling TR. Protein kinases. Regulation by autoinhibitory domains. J Biol Chem. 1990 Feb 5;265(4):1823–1826. [PubMed]
  • Tsutsumi-Ishii Y, Tadokoro K, Hanaoka F, Tsuchida N. Response of heat shock element within the human HSP70 promoter to mutated p53 genes. Cell Growth Differ. 1995 Jan;6(1):1–8. [PubMed]
  • Waldmann R, Hanson PI, Schulman H. Multifunctional Ca2+/calmodulin-dependent protein kinase made Ca2+ independent for functional studies. Biochemistry. 1990 Feb 20;29(7):1679–1684. [PubMed]
  • Waring RB, May GS, Morris NR. Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes. Gene. 1989 Jun 30;79(1):119–130. [PubMed]
  • Whitaker M, Patel R. Calcium and cell cycle control. Development. 1990 Apr;108(4):525–542. [PubMed]

Articles from Molecular Biology of the Cell are provided here courtesy of American Society for Cell Biology

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...