An FGF–WNT gene regulatory network controls lung mesenchyme development

doi:10.1016/j.ydbio.2008.04.009

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Dev Biol. Author manuscript; available in PMC 2009 October 6.

Published in final edited form as:

Dev Biol. 2008 July 15; 319(2): 426–436.

Published online 2008 June 3. doi: 10.1016/j.ydbio.2008.04.009.

PMCID: PMC2757945

NIHMSID: NIHMS77489

An FGF–WNT gene regulatory network controls lung mesenchyme development

Yongjun Yin,^a Andrew C. White,^a Sung-Ho Huh,^a Matthew J. Hilton,^b Hidemi Kanazawa,^a Fanxin Long,^a^b and David M. Ornitz^a^*

^aDepartment of Developmental Biology, Washington University School of Medicine, Campus Box 8103, 660 S. Euclid Avenue, St. Louis, MO 63110, USA

^bDepartment of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA

^*Corresponding author. Fax: +1 314 362 7058. E-mail address: Email: dornitz/at/wustl.edu (D.M. Ornitz)

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Abstract

Lung mesenchyme is a critical determinant of the shape and size of the lung, the extent and patterning of epithelial branching, and the formation of the pulmonary vasculature and interstitial mesenchymal components of the adult lung. Fibroblast growth factor 9 (FGF9) is a critical regulator of lung mesenchymal growth; however, upstream mechanisms that modulate the FGF mesenchymal signal and the downstream targets of mesenchymal FGF signaling are poorly understood. Here we have identified a robust regulatory network in which mesenchymal FGF signaling regulates β-Catenin mediated WNT signaling in lung mesenchyme. By conditionally inactivating β-Catenin in lung mesenchyme, we show that mesenchymal WNT-β-Catenin signaling is essential for lung development and acts to regulate the cell cycle G1 to S transition and the FGF responsiveness of mesenchyme. Together, both FGF and WNT signaling pathways function to sustain mesenchymal growth and coordinate epithelial morphogenesis during the pseudoglandular stage of lung development.

Keywords: Fibroblast growth factor 9 (FGF9), Fibroblast growth factor receptor (FGFR), Wnt2a, Wnt7b, β-Catenin, Lung development, Mesenchyme

Introduction

Mouse lung development is initiated on embryonic day 9.5 (E9.5) with the formation of two endodermal buds off the ventrolateral foregut. Lung buds invade the surrounding splanchnic mesoderm and overlying mesothelium to establish a lung primordium initially composed of three tissue layers, epithelium (endoderm), mesoderm and mesothelium (Hogan, 1999). As the lung buds grow and enter the pseudoglandular stage of development (E9.5–16), the mesenchyme forms two morphologically and molecularly distinct layers, the sub-mesothelial layer and the sub-epithelial layer (White et al., 2006). These mesenchymal layers express unique intercellular signaling molecules, such as WNT2a and FGF10 in the sub-mesothelial layer and Noggin in the sub-epithelial layer (Bellusci et al., 1996; Mailleux et al., 2005; Weaver et al., 2003; White et al., 2006). Growth and morphogenesis of the lung require intercellular signaling interactions between all primary cell layers of the lung (Cardoso and Lu, 2006; Shannon and Hyatt, 2004; Warburton et al., 2005).

Fgf9 is expressed in the outermost layer of the lung, the mesothelium, and in lung epithelium, and has been identified as a key factor that signals to mesenchyme to regulate proliferation, differentiation and the expression of other factors that in turn regulate epithelial development (Colvin et al., 1999; Colvin et al., 2001). Mesenchymal forms of FGF receptors 1 and 2 have been shown to mediate the FGF9 signal (White et al., 2006). Epithelial FGFs, such as FGF9, often regulate the expression of mesenchymal FGFs and other signaling molecules that in turn regulate epithelial and mesenchymal development. In a screen of candidate genes that are expressed in lung mesenchyme and that could be regulated by mesenchymal FGF signaling, Wnt2a was found to be significantly down-regulated in lungs of mice lacking Fgf9 (Fgf9^−/−) (see Fig. 1

). This suggested a link between FGF and WNT-β-Catenin signaling in lung mesenchyme and that regulation of WNT-β-Catenin signaling may be responsible for some of the lung phenotypes observed in Fgf9^−/− embryos.

Fig. 1

FGF9 regulates WNT signaling in lung mesenchyme. (A–H) Whole-mount in situ hybridization showing Wnt2a (A–D) and Lef1 (E–H) expression in E13.5 lung. All tissue pairs were hybridized and developed together in the same tube. Comparison (more ...)

WNT signaling is essential for embryonic patterning and cell fate determination (Moon, 2005). At least three intracellular signaling pathways mediate WNT signaling, the WNT-β-Catenin pathway, the WNT/Ca²⁺ pathway and the planar cell polarity (PCP) pathway. WNT-β-Catenin signaling through frizzled (FZ) receptors and low-density lipoprotein receptor-related protein (LRP) 5 and 6 co-receptors lead to activation of disheveled (DVL), inhibition of GSK3β, and stabilization and cytosolic accumulation of β-Catenin. Increased cytosolic levels of β-Catenin lead to its nuclear translocation and to the formation of active transcription factor complexes of β-Catenin with members of the T Cell Factor (LEF1, TCF1, TCF3, TCF4) transcription factor family, PITX2 and SOX17 (Brantjes et al., 2002; Briata et al., 2003; Kioussi et al., 2002; Vadlamudi et al., 2005; Zorn et al., 1999). Downstream targets of WNT-β-Catenin signaling include the cell cycle regulator, CyclinD1, and transcription factors, N-myc, Lef1 and Pitx2 (Hovanes et al., 2001; Kioussi et al., 2002; Shu et al., 2005; Tetsu and McCormick, 1999). In embryonic lung, nuclear β-Catenin, LEF1 and PITX2 have been detected in the epithelium and in adjacent mesenchyme (Hjalt et al., 2000; Mucenski et al., 2003; Tebar et al., 2001). Similarly, TOPGAL and BATGAL, WNT-β-Catenin reporter genes that detect a subset of sites of WNT-β-Catenin activity, are active in lung epithelium and in adjacent mesenchyme (De Langhe et al., 2005; Maretto et al., 2003; Okubo and Hogan, 2004; Pongracz and Stockley, 2006; Shu et al., 2005).

WNT-β-Catenin signaling has been investigated in lung epithelium through conditional inactivation of β-Catenin or overexpression of the WNT signaling antagonist dickkopf1 (DKK1). Both approaches resulted in defects in distal lung bud formation, possibly due to disruption of FGF10-FGFR2b signaling (Mucenski et al., 2003; Shu et al., 2005). Three Wnts (Wnt2a, Wnt2b, Wnt7b) that typically signal through the WNT-β-Catenin pathway are expressed during lung development. Inactivation of Wnt7b, which is expressed in distal lung epithelium, results in severe lung hypoplasia due to defects in branching morphogenesis, cell proliferation, epithelial differentiation and loss of vascular smooth muscle integrity (Shu et al., 2002). Wnt2a is highly expressed in the distal mesenchyme (Bellusci et al., 1996; Monkley et al., 1996) but Wnt2a targeted mice were reported to have normal lung development (Monkley et al., 1996). Wnt2b (formerly called Wnt13) is also expressed in lung mesenchyme during early stages of lung development (Katoh et al., 1996; Katoh et al., 2000; Zakin et al., 1998). This leaves open the possibility of functional redundancy between these two related and similarly expressed genes. Two Wnts (Wnt5a, Wnt11) that typically signal through non-canonical pathways are expressed in lung epithelium and mesenchyme. Wnt5a is expressed in lung epithelium and adjacent mesenchyme and inactivation of Wnt5a leads to over-branching of the epithelial airway and thickening of the mesenchymal interstitium (Li et al., 2005, 2002). Wnt11 is expressed both in the lung epithelium and mesenchyme, but its function during lung development is not clear (Lako et al., 1998).

Although WNT-β-Catenin signaling is clearly important for lung epithelial development, its role in lung mesenchyme has not been directly examined. Here we identify an essential role for mesenchymal WNT-β-Catenin signaling by conditionally inactivating β-Catenin specifically in lung mesenchyme. We furthermore identify a reciprocal signaling loop in lung mesenchyme in which mesothelial/epithelial to mesenchymal FGF signals regulate Wnt2a expression and WNT-β-Catenin signaling, and mesenchymal WNT-β-Catenin signaling regulates FGFR1 and FGFR2 expression and, consequently, the level of FGF signaling. Disruption of any component of this signaling network results in defects in lung mesenchymal development and consequent defects in epithelial morphogenesis.

Materials and methods

Mouse strains

All mouse strains, including Fgf9^+/−, β-Catenin^f/f, Dermo1-Cre, TRE-Fgf9-IRES-eGfp, SPC-rtTA, Fgfr1^f/f, Fgfr2^f/f (f indicates a floxed allele) and Rosa26 reporter (R26R), have been previously described (Brault et al., 2001; Colvin et al., 2001; Soriano, 1999; Tichelaar et al., 2000; Trokovic et al., 2003; White et al., 2006; Yu et al., 2003). Fgfr1/2^Dermo1 conditional knockout mice (Dermo1^Cre/+, Fgfr1^f/f, Fgfr2^f/f) and Fgf9^dox(48) mice (SPC-rtTA, TRE-Fgf9-IRES-eGfp) were made as described (Perl et al., 2002; White et al., 2006). The β-Catenin^f/f strain was acquired through the Jackson Laboratory (Bar Harbor, ME). For conditional inactivation of β-Catenin in lung mesenchyme, mice were generated with the genotype, Dermo1^Cre/+, β-Catenin^f/f (referred to as β-Catenin^Dermo1) by mating Dermo1^Cre/+, β-Catenin^f/+ mice with β-Catenin^f/f. Control mice were of the genotype Dermo1^Cre/+; Dermo1^Cre/+, β-Catenin^f/+; β-Catenin^f/f; or β-Catenin^f/+, all of which were phenotypically identical to wild type mice. All loss of function mice were maintained on a mixed 129SV/J-C57B6/J background. Transgenic strains, used for gain of function experiments, were maintained on the FVB background.

Analyses of mouse embryos, histology and immunohistochemistry

To induce FGF9 expression in vivo, the doxycycline-inducible epithelial transcriptional activator (SP-C-rtTA) was used to induce a tetracycline responsive transgene driving FGF9 (Perl et al., 2002; White et al., 2006). Doxycycline chow (Bio-Serv Inc., 300 mg/kg green pellets) was administered to pregnant female mice for 48 h prior to embryo isolation (Fgf9^dox(48)).

Embryo tissues were collected in ice-cold PBS, fixed in 4% PFA overnight at 4 °C, washed with PBS, photographed and embedded in paraffin prior to sectioning at 5 µm. For histology, slides were stained with hematoxylin and eosin (H&E). For immunohistochemistry, paraffin-embedded or cryo-sections were rehydrated and treated with 0.3% hydrogen peroxide in methanol for 15 min to suppress the endogenous peroxidase activity. Antigen retrieval was achieved by microwaving the sections in 10 mM citrate buffer for 5 min followed by gradual cooling to room temperature. Sections were incubated overnight at 4 °C with the following primary antibodies: PCNA (sc-56, Santa Cruz Biotechnology Inc, 1:100); β-Catenin (610153, BD Transduction Laboratories, 1:200); LEF1 (sc-8591, Santa Cruz Biotechnology Inc, 1:100); BrdU (347580, Becton Dickinson Immunocytometry Systems, 1:200); phospho-histone H3 (pHH3, H9908, Sigma, 1:100); FGFR2 (sc-122, Santa Cruz Biotechnology Inc, 1:100); CyclinD1 (#2926, Cell Signaling Technology, 1:100); phospho-ERK (M8159, Sigma, 1:100), and TTF-1 (M3575, DAKO Cytomation, 1:100), visualized using Broad Spectrom (AEC) Kit (95-9743, Zymed Laboratories Inc.) or Vectastain® Elite ABC (AEC) kit (PK-4005, Vector Laboratories).

For BrdU analysis, pregnant females were injected with BrdU at 0.1 mg/kg body weight, 2 h prior to harvest. Embryos were collected in ice-cold PBS, processed and sectioned as above.

All staining patterns are representative of at least three embryos.

Cell death analysis

Paraffin or cryo-sectioned slides, prepared as described above, were assayed (TUNEL) using the In Situ Cell Death Detection Kit (Roche Applied Science). Slides were mounted with DAPI containing Vectashield® mounting medium (H-1200, Vector Laboratories) for fluorescent detection or counterstained with hematoxylin (MHS-16, Sigma) for AEC detection and then photographed. All staining patterns are representative of at least three embryos.

Whole-mount in situ hybridization

In situ probes were from the following sources: Lef1, Wnt2a (A. McMahon), Wnt7b (F. Long); Fgf10 (B. Hogan); Spry2; Fgfr1 (S. Werner); N-Myc (E. Morrisey). Probes were synthesized and labeled with a kit from Roche Applied Science. Whole-mount in situ hybridization was performed as described (Colvin et al., 2001). Following color reaction and methanol dehydration, tissues were photographed and then cryo-sectioned (6 µm), mounted on slides and re-photographed. In situ hybridizations of tissue sections were performed as previously described (Colvin et al., 1999). All staining patterns are representative of at least three embryos.

Whole-mount LacZ staining

Lungs were dissected in ice-cold PBS and fixed with 0.5% glutaraldehyde in PBT (PBS, 0.1% Tween-20) overnight at 4 °C. Tissues were washed in PBT twice for 10 min prior to incubation with LacZ staining solution (2 mM MgCl₂, 35 mM potassium ferrocyanide, 35 mM potassium ferricyanide, 1 mg/ml X-Gal in PBT) at room temperature in the dark. Following adequate color reaction, tissues were again washed twice in PBT for 10 min each to stop the reaction. Samples were then soaked in 30% sucrose overnight, photographed and then embedded and frozen in OTC for cryosectioning (6 µM). Sections were dried for ~3 h at room temperature, washed with PBS and mounted. All staining patterns are representative of at least three embryos.

Lung organ cultures

Lung explant cultures were performed as described (White et al., 2006). E11.5 control, Fgf9^−/− and β-Catenin^Dermo1 embryonic lungs were dissected and cultured on Transwell filters (Costar, Corning) for 48 h at 37 °C, 5% CO₂. Mouse FGF9 protein (PeproTech Inc.) was used at a concentration of 2.5 ng/ml in DMEM supplemented with 2 µg/ml heparin. LiCl was used at a concentration of 20 mM (Dean et al., 2005). To quantify mesenchymal thickness, explants were photographed and mesenchymal thickness was measured using Canvas X software. Data shown is representative of at least three independent experiments.

RNA isolation, cDNA synthesis, and qRT-PCR analysis

E14.5 embryonic lung mRNA was isolated using the RNeasy kit (Qiagen Inc.) following manufacturer's instructions. cDNA was synthesized using the SuperScript II first-strand cDNA synthesis kit (Invitrogen). Quantitative RT-PCR was performed on an ABI 7500 machine using TaqMan® probes for Gapdh and Fgfr2. Amplification and analysis were performed according to the manufacturer's instructions. All reactions were normalized to Gapdh. Results were graphed as relative expression compared with control, where control was scaled to 1.

Construction of a Fgfr2 promoter-luciferase reporter

A mouse genomic DNA fragment extending 6.8 kb 5′ and 533 bp 3′ to the Fgfr2 transcription initiation site was excised from BAC DNA (RP23-466 J2) with BamH1 and subcloned into the pGL3 basic vector (Promega Inc.) to generate pGL3-Fgfr2p. This fragment contains Fgfr2 exon 1 and part of intron 1. The pCIG-dominant active β-Catenin vector (0.2 µg) or control vector (pCIG) (Megason and McMahon, 2002) was co-transfected into HEK293 cells with pGL3-Fgfr2p and pCMV-β-Gal (Promega) using Fugene 6 (Roche Applied Science). Luciferase assay was performed 48 h after transfection using the Luciferase Assay System (Promega). All data were normalized to β-Gal activity. Data represents four independent experiments. Positive controls (not shown), using the TOPFLASH reporter, demonstrated activation of WNT signaling.

Results

FGF9 regulates WNT signaling in lung mesenchyme

Wnt2a is prominently expressed in lung mesenchyme and has been shown to signal through the WNT-β-Catenin pathway (Bellusci et al., 1996; Karasawa et al., 2002). Examination of E13.5 lung tissue from mice lacking FGF9 (Fgf9^−/− mice) demonstrated a complete absence of Wnt2a expression in lung mesenchyme (Figs. 1A, B

). By contrast, induced overexpression of FGF9 in lung epithelium from E11.5 to E13.5 (Fgf9^dox(48)) (White et al., 2006) resulted in increased Wnt2a expression (Figs. 1C, D

). To determine if WNT-β-Catenin signaling was regulated by FGF9 in lung mesenchyme, we examined the expression of Lef1, a transcriptional target of the WNT-β-Catenin pathway that is expressed in lung mesenchyme (Porfiri et al., 1997; Tebar et al., 2001). In Fgf9^−/− mice, Lef1 expression was reduced in lung mesenchyme at E13.5, whereas in mice overexpressing Fgf9 in lung epithelium from E11.5 to 13.5, Lef1 expression was increased in the sub-epithelial mesenchymal domain (Figs. 1E–H

). Immunohistochemical detection of LEF1 revealed a similar pattern and regulation in response to gain and loss of expression of Fgf9 (Supplemental Fig. 1). To determine whether FGF9 signaling has any effect on an epithelial Wnt, we examined the expression of the WNT-β-Catenin pathway ligand, Wnt7b (Shu et al., 2002). Expression of Wnt7b was not affected in either FGF9 loss of function or gain of function mouse models (Figs. 1I–L

). These data suggest that Wnt2a may contribute to WNT-β-Catenin signaling in lung mesenchyme and is a candidate for a downstream target of FGF9 signaling.

FGF receptors 1 and 2 have been shown to act redundantly to mediate the FGF9 signal in lung mesenchyme (White et al., 2006).We thus hypothesized that inhibition of mesenchymal FGF receptors should also result in decreased mesenchymal WNT-β-Catenin signaling. To test this hypothesis directly, we conditionally inactivated floxed alleles of both Fgfr1 and Fgfr2 in the lung mesenchyme with Dermo1-Cre and then examined the expression of Wnt2a and Lef1. Dermo1-Cre is active in lung mesenchyme and mesothelium, but not epithelium. By E10.5, Dermo1-Cre can efficiently activate the ROSA26 reporter allele (R26R) and β-galactosidase activity throughout lung mesenchyme and mesothelium (see Fig. 3J

below). Consistent with our previous report (White et al., 2006), Dermo1^cre/+, Fgfr1^f/f, Fgfr2^f/f (hereafter referred to as Fgfr1/2^Dermo1) lungs were smaller than control lungs beginning at E12.5. Whole-mount in situ hybridization of E12.5 Fgfr1/2^Dermo1 lungs showed reduced Wnt2a (Figs. 2A, B

) and Lef1 expression in lung mesenchyme (Figs. 2C, D

), similar to what was observed in Fgf9^−/− mice. Consistent with decreased FGFR signaling, mesenchymal proliferation was decreased at E14.5 (Figs. 2E, F

). Together, these data suggest that FGFR signaling in lung mesenchyme modulates the level of mesenchymal WNT-β-Catenin signaling in lung mesenchyme.

Fig. 3

Mesenchymal β-Catenin is essential for embryonic lung development. (A–C) Anterior views of gross dissections of control (Dermo1-Cre, β-Catenin^f/+) and β-Catenin^Dermo1 lungs at E12.5 (A), E14.5 (B) and E18.5 (C). Note the (more ...)

Fig. 2

Mesenchymal FGFR signaling regulates mesenchymal WNT-β-Catenin signaling and mesenchymal proliferation. (A–D) WNT-β-Catenin signaling in control and Fgfr1/2^Dermo1 lung mesenchyme. Whole-mount in situ hybridization of Wnt2a (A, (more ...)

Mesenchymal β-Catenin is essential for embryonic lung development

To directly investigate the role of mesenchymal WNT-β-Catenin signaling in embryonic lung development, β-Catenin (β-Catenin ^f/f) (Brault et al., 2001) was conditionally targeted in lung mesenchyme with Dermo1-Cre (Yu et al., 2003). Mice of the genotype Dermo1^Cre/+, β-Catenin^f/f, hereafter referred to as β-Catenin^Dermo1, showed impaired lung growth as early as E12.5 (Fig. 3A

). Control mice of the genotype Dermo1^Cre/+, β-Catenin^f/+ or Dermo1^+/+, β-Catenin^f/f were phenotypically normal (Fig. 3A

and data not shown). β-Catenin^Dermo1 lungs had a normal number and orientation of lobes but fewer epithelial branches and decreased mesenchyme. In some cases, lobes were misshapen and bent. By E14.5, the lungs were less than half of the normal size and failed to grow during the remainder of embryonic development (Fig. 3B

). By E18.5, β-Catenin^Dermo1 lungs were small and compact, lacked smooth borders, and the trachea had completely degenerated (Fig. 3C

). β-Catenin^Dermo1 mice died at birth due to respiratory failure. Histologic sections of E12.5 and E14.5 β-Catenin^Dermo1 lung tissue revealed decreased epithelial branching and decreased distal mesenchyme, but otherwise normal appearing mesenchymal and epithelial compartments (Figs. 3D–G

). At E18.5, control lungs had clear airspace development, accompanied by thinning of the mesenchyme and close approximation of fetal capillaries to flattened epithelial cells. By contrast, β-Catenin^Dermo1 lung tissue showed poorly organized peripheral lung tissue without well-formed air sacs (Figs. 3H, I

To examine the efficiency and specificity of β-Catenin inactivation, immunohistochemistry was used to localize β-Catenin protein. Control lung tissue showed uniform staining for β-Catenin in both epithelium and mesenchyme, while β-Catenin^Dermo1 lungs showed greatly reduced staining in lung mesenchyme as early as E12.5 (Figs. 3K, L

) and complete absence of mesenchymal protein at E14.5 (Figs. 3M, N

) and E18.5 (Supplemental Fig. 2). Epithelial β-Catenin expression appeared unchanged throughout lung development. To determine whether mesenchymal WNT signaling was reduced as a consequence of inactivation of β-Catenin, expression of the WNT-β-Catenin regulated genes, Lef1, N-Myc and CyclinD1 (see below), was examined. Expression of Lef1 was decreased in β-Catenin^Dermo1 lung mesenchyme at E12.5 (Figs. 4A–D

). Immunohistochemistry revealed an absence of LEF1 protein in lung mesenchyme at E12.5 in β-Catenin^Dermo1 lungs (Figs. 4E, F

). These data demonstrate down-regulation of mesenchymal WNT-β-Catenin signaling following inactivation of β-Catenin in lung mesenchyme. Interestingly, Wnt2a expression was also reduced in β-Catenin^Dermo1 lungs (Figs. 4G, H

), suggesting the presence of a feedback loop downstream of β-Catenin that regulates WNT-β-Catenin signaling (see below).

Fig. 4

Mesenchymal and epithelial WNT-β-Catenin signaling following conditional inactivation of β-Catenin in lung mesenchyme. (A–D) Expression of Lef1 in control (A, C) and β-Catenin^Dermo1 lung mesenchyme (B, D) at E12.5. A,B (more ...)

To determine whether lung epithelial WNT-β-Catenin signaling was affected by inactivation of β-Catenin in lung mesenchyme, N-Myc expression was examined. N-Myc is expressed in lung epithelium and is a gene known to be regulated by epithelial WNT-β-Catenin signaling (Shu et al., 2005). In situ hybridization analysis showed that, at E12.5, N-Myc expression was not significantly affected by inactivation of lung mesenchymal β-Catenin (Figs. 4I, J

). Additionally, Wnt7b, a WNT ligand expressed in lung epithelium that signals through canonical WNT-β-Catenin pathways, showed slightly increased expression in β-Catenin^Dermo1 lung epithelium compared to wild type controls (Figs. 4K, L

Mesenchymal β-Catenin is required for proliferation and survival of lung mesenchymal and epithelial cells

The smaller size of β-Catenin^Dermo1 lungs between E12.5 and E14.5, and the failure to grow after E14.5, suggested that inactivation of β-Catenin may lead to decreased proliferation or increased cell death in lung mesenchyme and may secondarily affect growth and survival of lung epithelium. We assessed cell proliferation by examining expression of proliferating cell nuclear antigen (PCNA), as well as incorporation of bromodeoxyuridine (BrdU). PCNA labels all cycling cells, while BrdU specifically labels cells that are within S phase during the labeling period. At E12.5 and E14.5, BrdU labeling was significantly decreased in β-Catenin^Dermo1 lung tissue compared to control (Figs. 5A–D

). Quantitation revealed decreased proliferation within both the epithelial and mesenchymal compartments (Table 1). To further examine cell cycle progression, we examined the level of phosphor-histone H3 (pHH3), a marker expressed during G2/M, by immunohistochemistry. At E12.5, pHH3 labeling was significantly decreased in β-Catenin^Dermo1 lung mesenchyme, but not in epithelium(Figs. 5E, F

, Table 1). By contrast, at E14.5, pHH3 labeling was significantly decreased in β-Catenin^Dermo1 lung in both mesenchyme and epithelium (Figs. 5G, H

, Table 1). Surprisingly, despite severe hypoplasia of β-Catenin^Dermo1 lung tissue, there was an apparent increase in the PCNA labeling index (number of PCNA-positive nuclei/total nuclei) in both the mesenchymal and epithelial compartments at E14.5 (Table 1, Supplemental Fig. 3), suggesting an arrest in the cell cycle at the G1/S boundary and accumulation of PCNA-positive, G1/S arrested cells. Together, the data suggest that removal of mesenchymal β-Catenin results in an initial significant reduction in the proliferation rate of mesenchymal cells and an apparent secondary defect in epithelial proliferation.

Fig. 5

Mesenchymal β-Catenin is required for proliferation and survival of lung mesenchymal and epithelial cells. (A–D) BrdU labeling was significantly decreased in β-Catenin^Dermo1 lung tissue at E12.5 (B) and E14.5 (D) compared to control (more ...)

Table 1

Quantification of proliferation markers in control and β-Catenin^Dermo1 lungs

D type Cyclins are required for progression through the cell cycle (Baldin et al., 1993) and their expression is controlled by extracellular growth factors, including members of the WNT and FGF family (Issack and Ziff, 1998; Lobjois et al., 2004). At E12.5 and E14.5, Cyclin D1 expression was reduced in β-Catenin^Dermo1 lung mesenchyme and epithelium (Figs. 5I–L

, Table 1). The decrease in epithelium appeared greater at E14.5 (Figs. 5K, L

Cell death was examined by TUNEL labeling. At E12.5 and E14.5, no significant increase in cell death was detected in β-Catenin^Dermo1 lung tissue (Figs. 5M–P

). However, at E18.5, massive apoptosis was apparent throughout lung epithelium and mesenchyme (Figs. 5Q, R

). These data indicate that mesenchymal WNT-β-Catenin signaling is either directly required for cell survival after E14.5, that proper WNT-β-Catenin signaling at earlier stages is necessary for lung viability at later times in development, or that other phenotypes affect lung survival at later stages of development. Further studies were therefore focused on earlier developmental stages (E12.5–E14.5) to identify more direct functions of WNT-β-Catenin signaling in lung mesenchyme.

Taken together, these data demonstrate that mesenchymal WNT-β-Catenin signaling is essential for maintaining lung mesenchymal proliferation. This could be a direct consequence of WNT regulation of cell cycle genes or an indirect effect mediated through regulation of other signaling pathways. Additionally, non-autonomous effects on lung epithelial proliferation suggest loss of a mesenchymal-derived signal to epithelial cells.

Epithelial identity and signaling in β-Catenin^Dermo1 lung

To further examine lung epithelial function in β-Catenin^Dermo1 lung, Thyroid Transcription Factor 1 (TTF1) and Surfactant Protein-C (SP-C) expression was examined. TTF1 is essential for epithelial cell development (Cardoso and Lu, 2006) and SP-C is expressed in distal lung epithelium (Weaver et al., 1999). Immunostaining for TTF1 demonstrated expression in epithelial cell nuclei at near normal levels at E12.5 and E14.5 in β-Catenin^Dermo1 lung (Figs. 6A–D

), similar to what was observed in Fgfr1/2^Dermo1 lung (data not shown). Expression of SP-C was reduced in β-Catenin^Dermo1 lung at E12.5 (data not shown).

Fig. 6

Deficiency of lung mesenchymal WNT-β-Catenin signaling does not affect epithelial FGF signaling. (A–D) Immunostaining shows comparable TTF1 expression in the epithelium of control (A, C) and β-Catenin^Dermo1 lung (B, D) at E12.5 (more ...)

FGF10 is an essential factor for lung epithelial branching. Focal Fgf10 expression is observed in mesenchyme at the distal tip of the prospective lung lobes starting at ~E10.0 and in distal mesenchyme adjacent to budding airway epithelium at later stages (Bellusci et al., 1997). Expression patterns of Fgf10 were examined to determine whether altered FGF10 expression could account for some of the epithelial phenotypes observed in β-Catenin^Dermo1 lung. At E12.0 and E14.5, control and β-Catenin^Dermo1 lungs exhibited comparable expression patterns and levels of Fgf10 in mesenchyme distal to budding airways (Figs. 6E–H

). To examine FGF signaling in lung epithelium, Sprouty 2 (Spry2) expression was examined. Spry2 is one of the earliest targets to be induced in the lung epithelium in response to FGF10 (Mailleux et al., 2001) and is a negative regulator of the FGF signaling pathway. Comparison of Spry2 expression showed no significant difference in control and β-Catenin^Dermo1 lungs at E12.5 and E14.5 (Figs. 6I–L

), further suggesting that FGF10-FGFR2b signaling is not a primary target of mesenchymal WNT-β-Catenin signaling.

WNT-β-Catenin signaling regulates FGF receptor expression and function in lung mesenchyme

Mesenchymal proliferation is decreased in Fgf9^−/−, Fgfr1/2^Dermo1 and in β-Catenin^Dermo1 lung tissue. We hypothesized that the proliferation defect in β-Catenin^Dermo1 lung mesenchyme might be mediated in part through regulation of FGF signaling. Fgfr1 and FGFR2 expression was therefore examined. Immunostaining of E12.5 lung showed decreased expression of FGFR2 in β-Catenin^Dermo1 lung mesenchyme but not in epithelium (Figs. 7A, B

). At E14.5, FGFR2 expression was not detectable in lung mesenchyme but still was expressed at normal levels in distal epithelium (Figs. 7C, D

Fig. 7

WNT-β-Catenin signaling regulates FGF receptor expression in lung mesenchyme. (A–D) Immunostaining of E12.5 and E14.5 lung showing FGFR2 expression in control lung mesenchyme and epithelium (A, C) and the absence of FGFR2 expression in (more ...)

To further examine the regulation of lung mesenchymal Fgfr2 expression by mesenchymal WNT-β-Catenin signaling, we examined expression of Fgfr2 in E14.5 lung tissue from control and β-Catenin^Dermo1 mice by quantitative RT-PCR. In the absence of mesenchymal WNT-β-Catenin signaling, Fgfr2 showed 4.6 fold lower expression (Fig. 7E

). To further test the ability of WNT-β-Catenin signaling to regulate Fgfr2 gene expression, wild type lung explants were treated with 20 mM LiCl for 48 h. LiCl inhibits GSK3β and stabilizes and activates cytosolic β-Catenin (Klein and Melton, 1996). Quantitative RT-PCR showed that in the presence of LiCl, Fgfr2 expression was increased 6.8 fold (Fig. 7F

). Finally, 6.8 kb of Fgfr2 genomic sequence, located 5′ to the transcription initiation site, was cloned into a luciferase expression plasmid, pGL3 basic. Co-transfection with pCIG-dominant active β-catenin, containing a constitutively active form of β-Catenin, resulted in a 4.6 fold increase in luciferase activity relative to controls (co-transfection with pCIG) (Fig. 7G

Fgfr1 mRNA expression was examined by whole-mount in situ hybridization. Fgfr1 showed reduced expression in lung mesenchyme at E12.5 (Figs. 7H, I

) and E14.5 (Figs. 7J, K

). Epithelial expression was also decreased at E14.5 in β-Catenin^Dermo1 lungs (Fig. 7K

). Consistent with decreased FGFR expression and decreased mesenchymal proliferation, p-ERK expression was decreased throughout β-Catenin^Dermo1 lungs at E14.5 (Figs. 7L, M

, Table 1).

If decreased mesenchymal FGFR expression in β-Catenin^Dermo1 lung was functionally important, one would predict that β-Catenin^Dermo1 lungs would have impaired responsiveness to FGF ligands that primarily signal to mesenchymal FGFRs. To test this, lung explant cultures from control and β-Catenin^Dermo1 lungs were treated with BSA or FGF9 (Figs. 8A–E

). Control lung explants responded to FGF9 with mesenchymal hyperplasia, as we have previously reported (White et al., 2006) (Figs. 8A, B

). Measurement of mesenchymal thickness in control lung explants showed a 2.8 fold (p<0.01) increase in response to FGF9 (Fig. 8E

). In contrast, β-Catenin^Dermo1 lung explants showed no significant mesenchymal response to FGF9, and the sub-mesothelial mesenchymal layer remained thin, similar to BSA treated explants (Figs. 8C–E

). Of note, epithelium in both control and β-Catenin^Dermo1 lungs showed a dilation in response to FGF9 (Fig. 8B, D

), consistent with the possibility that FGF9 may also signal directly to lung epithelium (del Moral et al., 2006).

Fig. 8

β-Catenin^Dermo1 and Fgf9^−/− lung mesenchyme have a decreased response to FGF9. (A–D) Lung explant cultures from control (A, B) and β-Catenin^Dermo1 lungs (C, D) were treated with BSA (A, C) or FGF9 (B, D). Control (more ...)

Another prediction that arises from this regulatory network model is that, in the absence of FGF9, the feedback loop that maintains WNT-β-Catenin signaling and Fgfr expression should degrade and lung explants from Fgf9^−/− embryos should not be able to be rescued by treatment with FGF9. Consistent with this prediction, Fgf9^−/− lung explants showed no mesenchymal response to FGF9 (Figs. 8H–J

), while control mesenchyme showed a 2.7 fold increase in mesenchymal thickness in response to FGF9 (Figs. 8F, G, J

). Epithelium from both control and Fgf9^−/− explants showed epithelial dilation in response to FGF9 (Figs. 8F–I

Discussion

We have identified a robust regulatory circuit in which epithelial to mesenchymal FGF signaling regulates the expression of a Wnt ligand and the level of WNT-β-Catenin signaling, and in which mesenchymal WNT-β-Catenin signaling regulates the expression of FGF receptors and the level of mesenchymal FGF signaling (Fig. 9

). The only external (non-mesenchymal) factor that has thus far been identified that can modulate this signaling system is FGF9, which is produced by the mesothelial lining of the lung and by airway epithelium, and potentially WNT7b, which is produced in airway epithelium. The primary output of this system appears to be regulation of mesenchymal proliferation, but clearly other mesenchymal factors that regulate lung epithelial proliferation, survival and differentiation must also act downstream of FGF and/or β-Catenin.

Fig. 9

Molecular pathways by which WNT-β-Catenin and FGF regulate lung mesenchymal development. (1) At early stages of lung development (E10–E12), FGF9 is produced by lung mesothelium and epithelium and signals to mesenchymal FGFR1c and FGFR2c (more ...)

Two predictions can be derived from the FGF–WNT interaction model (Fig. 9

). First: down-regulation or inhibition of any component of this system should diminish the output of the entire system. Second: up-regulation of any component of the system should reinforce the entire system. In vitro organ culture experiments and in vivo genetic experiments support the circular and self-sustaining nature of this signaling network. For example, lung tissue lacking mesenchymal β-Catenin was predicted and shown to down-regulate mesenchymal responsiveness to FGF9. Similarly, loss of mesenchymal FGF signaling resulted in diminished WNT-β-Catenin signaling, loss of Fgfr expression and inability of the tissue to be rescued by treatment with FGF9. However, phenotypic differences resulting from loss of either mesenchymal β-Catenin or mesenchymal FGFR1 and FGFR2 indicate that other factors must also feed into this signaling circuit to sustain mesenchymal viability. Apparent differences could also be due to variation in the timing or efficiency of Cre-mediated inactivation of β-Catenin and Fgfrs 1 and 2, versus complete loss of mesenchymal FGF signaling in Fgf9^−/− lungs. In FGF loss of function models (Fgf9^−/− and Fgfr1/2^Dermo1), the lung becomes atrophic by birth but does not exhibit widespread cell death (Colvin et al., 2001; White et al., 2006). By contrast, β-Catenin^Dermo1 lungs exhibit widespread apoptosis by E18.5. These observations provide further evidence that these two pathways are not equivalent and that β-Catenin signaling may have a more important role in mesenchymal cell survival. Furthermore, some intrinsic or extrinsic signal(s) likely maintains low-level mesenchymal β-Catenin signaling and cell viability in the absence of FGF signaling. This is supported by the observed reduction, but not elimination, of WNT-β-Catenin activity (Lef1 expression) in Fgf9^−/− lung mesenchyme. However, besides FGF9 and WNT7b, factors that regulate mesenchymal WNT-β-Catenin signaling have not been identified.

Wnt7b is expressed in lung epithelium (Pepicelli et al., 1998; Weidenfeld et al., 2002). Wnt7b^−/− embryos have reduced lung mesenchymal proliferation and defects in lung vascular smooth muscle (Shu et al., 2002). It is, therefore, likely that WNT7b signals directly to adjacent sub-epithelial mesenchyme and acts synergistically or redundantly with WNT2a (Fig. 9

). Wang et al. (2005) have shown that WNT7b can activate FZD1, 4 and 7 and that these WNT receptors are expressed in lung mesenchyme and in vascular smooth muscle precursors; however, direct targets of WNT-β-Catenin signaling were not examined in lung mesenchyme of Wnt7b^−/− mice. Supporting the idea that WNT7b could regulate mesenchymal WNT-β-Catenin activity independent of FGF9, we showed that the expression of Wnt7b was increased in Fgf9^−/− lungs. Thus, WNT7b is likely to be a second independent external factor that can modulate the mesenchymal WNT-β-Catenin–FGFR regulatory circuit.

Lef1, a target of WNT-β-Catenin signaling, appears to be uniformly expressed throughout distal mesenchyme. This suggests that Wnt2a, which is expressed in sub-mesothelial mesenchyme, might support both autocrine signaling to sub-mesothelial mesenchyme and paracrine signaling to sub-epithelial mesenchyme. Although a lung mesenchymal phenotype was not reported for Wnt2a^−/− mice (Monkley et al., 1996) and the original knockout line was not saved, a second, recently constructed line of Wnt2a^−/− mice shows decreased lung mesenchymal proliferation (E. Morrisey, personal communication). Additionally, Wnt7b^−/− mice show decreased lung mesenchymal proliferation (Shu et al., 2002). These data support a potential role for these ligands acting together in the regulation of mesenchymal WNT-β-Catenin signaling. To demonstrate this in vivo, future epistasis studies will be required in which Wnt2a and Wnt7b double heterozygous mice are examined.

To address the contribution of WNT-β-Catenin signaling in lung epithelium, several labs have conditionally inactivated β-Catenin in epithelium or overexpressed the WNT antagonist, DKK, in epithelium (De Langhe et al., 2005; Mucenski et al., 2003; Shu et al., 2005). These studies showed that WNT-β-Catenin signaling regulates the expression of N-Myc, Bmp4 and Fgfr2b in lung epithelium and regulates the extent of branching morphogenesis. Down-regulation of epithelial Fgfr2b could account for the observed defects in branching morphogenesis in these mice. Importantly, the identity of the WNT ligand(s) that regulates epithelial WNT-β-Catenin signaling is not known; however, in addition to activating FZD1, 4 and 7 in lung mesenchyme, WNT7b can activate FZD10, which is expressed in lung epithelium (Wang et al., 2005). A possible autocrine role for WNT7b in epithelium is supported by observed defective epithelial differentiation in Wnt7b^−/− lung tissue (Shu et al., 2002).

In summary, FGF9, and most likely WNT7b, are two ligands that can independently signal from mesothelial (FGF9) and epithelial (FGF9 and WNT7b) cells to lung mesenchyme to regulate growth. The positive feedback loop within the mesenchymal compartment (Wnt2a, FGFR1, FGFR2 and β-Catenin) allows input from both FGF and WNT signaling systems to modulate the output of the entire system, thus providing a mechanism to tightly regulate mesenchymal lung development and, indirectly, epithelial morphogenesis.

Supplementary Material

Supplementary data: Appendix A. Supplementary data

Supplementary data associated with this article can be found, in the online version, at doi:10.1016/j.ydbio.2008.04.009.

Click here to view.^{(1.4M, pdf)}

Acknowledgments

We thank J. Partanen for the Fgfr1^f/f mouse line. We thank C. Smith and G. Schmid for animal husbandry and genotyping. This work was funded by the Washington University Cardiovascular Pharmacology Training Grant T32 HL07873 (ACW), the Digestive Diseases Research Core Center Grant P30 DK052574 (transgenic mouse production) and a grant from the March of Dimes Foundation (1FY06-339).

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