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J Clin Microbiol. 1978 Jul; 8(1): 36–41.
PMCID: PMC275110

Improved media for growth and aerotolerance of Campylobacter fetus.


The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% O2, 17% O2, and 21% O2, respectively. Unsupplemented brucella agar supported growth of 94, 48, and 20% of the strains. Brucella broth supplemented with 0.2% FeSO4.7H2O, 0.025% sodium metabisulfite, and 0.05% sodium pyruvate supported growth of 98% of the strains at 21% O2, compared to 75% with unsupplemented brucella broth. With both the supplemented agar and broth, growth responses occurred 1 to 2 days earlier than usual. Growth and aerotolerance of three strains of Campylobacter sputorum subsp. bubulus were not enhanced by the supplements.

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Selected References

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  • Border MM, Firehammer BD, Myers LL. Tube culture method for viable counts of Campylobacter fetus (Vibrio fetus). Appl Microbiol. 1974 Oct;28(4):730–732. [PMC free article] [PubMed]
  • Bowdre JH, Krieg NR, Hoffman PS, Smibert RM. Stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of Spirillum volutans and Campylobacter fetus subspecies jejuni. Appl Environ Microbiol. 1976 Jan;31(1):127–133. [PMC free article] [PubMed]
  • KIGGINS EM, PLASTRIDGE WN. Effect of gaseous environment on growth and catalase content of Vibrio fetus cultures of bovine origin. J Bacteriol. 1956 Sep;72(3):397–400. [PMC free article] [PubMed]
  • KINGSCOTE B. Use of catalase in the culture of Vibrio fetus. Can J Microbiol. 1961 Dec;7:951–952. [PubMed]
  • Niekus HG, de Vries W, Stouthamer AH. The effect of different dissolved oxygen tensions on growth and enzyme activities of Campylobacter sputorum subspecies bubulus. J Gen Microbiol. 1977 Dec;103(2):215–222. [PubMed]
  • Van Palenstein Helderman WH, Rosman I. Hydrogen-dependent organisms from the human gingival crevice resembling Vibrio succinogenes. Antonie Van Leeuwenhoek. 1976;42(1-2):107–118. [PubMed]

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