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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nature. Author manuscript; available in PMC Jan 23, 2010.
Published in final edited form as:
PMCID: PMC2733940
NIHMSID: NIHMS120632

Ago HITS-CLIP decodes miRNA-mRNA interaction maps

Summary

MicroRNAs (miRNAs) play critical roles in the regulation of gene expression. However, since miRNA activity requires base pairing with only 6-8 nucleotides of mRNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native Argonaute (Ago) protein-RNA complexes in mouse brain. This produced two simultaneous datasets—Ago-miRNA and Ago-mRNA binding sites—that were combined with bioinformatic analysis to identify miRNA-target mRNA interaction sites. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.

Sophisticated mechanisms regulating RNA may explain the gap between the great complexity of cellular functions and the limited number of primary transcripts. Regulation by microRNAs (miRNAs) underscore this possibility, as miRNAs are each believed to directly bind to many mRNAs to regulate their translation or stability1,2, and thereby control a wide range of activities, including development, immune function and neuronal biology3-5. Many miRNAs are evolutionarily conserved, although others are species-specific (including human miRNAs not conserved in chimpanzees)6, consistent with roles ranging from generating cellular to organismal diversity.

Despite their biologic importance, determining miRNA targets is major challenge. The problem stems from the discovery that functional mRNA regulation requires interaction with as few as 6 nucleotides (nt) of miRNA seed sequence7. Such 6-mers are present on average every ~4 kb, so that miRNAs could regulate a broad range of targets, but the full extent of their action is not known. Bioinformatic analysis has greatly improved the ability to predict bona fide miRNA binding sites8-10, principally by constraining searches for evolutionary conserved seed matches in 3′ UTR. Nonetheless, different algorithms produce divergent results with high false positive rates3,11,12. In addition, many miRNAs are present in closely related miRNA families, complicating interpretation of loss of function studies in mammals13,14, although such studies have been informative for several miRNAs3,15-17. miRNA overexpression or knockdown studies, most recently in combination with proteomic studies11,18, have led to the conclusion that individual miRNAs generally regulate a relatively small number of proteins at modest levels (< 2-fold), although the false-positive rate of target predictions remains high (~up to 66%)11, and the data sets analyzed have been of limited size (~5,000 proteins). Similar high false-positive rates have been observed when miRNAs were co-immunoprecipitated with Ago proteins19-23. A critical caveat common to all of these studies is their inability to definitively distinguish direct from indirect miRNA-target interactions. At the same time, as therapeutic antisense strategies become more viable17,24,25, knowledge of direct miRNA target sites has become increasingly important.

Recently, we developed HITS-CLIP to directly identify protein-RNA interactions in living tissues in a genome-wide manner26,27. This method28,29 uses UV-irradiation to covalently crosslink RNA-protein complexes that are in direct contact (~single Angstrom distances) within cells, allowing them to be stringently purified. Partial RNA digestion reduces bound RNA to fragments that can be sequenced by high throughput methods, yielding genome wide maps and functional insights26,30. Recent X-Ray crystal structures of an Ago-miRNA-mRNA ternary complex31 suggest that Ago may make sufficiently close contacts to allow Ago HITS-CLIP to simultaneously identify Ago bound miRNAs and the nearby mRNA sites. Here we use Ago HITS-CLIP to define the sites of Ago interaction in vivo, decoding a precise map of miRNA-mRNA interactions in the mouse brain. This provides a platform that can establish the direct targets upon which miRNAs act in a variety of biologic contexts, and the rules by which they do so.

Ago RNA targets in the mouse brain

HITS-CLIP experiments rely on a means of purifying RNA binding proteins (RNABPs)26-29. To purify Ago bound to mouse brain RNAs, we UV-irradiated P13 neocortex and immunoprecipitated Ago under stringent conditions. After confirming the specificity of Ago immunoprecipitation (Fig. 1a), we radiolabeled RNA, further purified crosslinked Ago-RNA complexes by SDS-PAGE and nitrocellulose transfer, and visualized them by autoradiography. We observed complexes of two different modal sizes (~110 kD and ~130kD; Fig. 1b and Supplementary Fig. 1), suggesting that Ago (97kD) was crosslinked to two different RNA species. RT-PCR amplification revealed that the ~110 kD band harbored ~22 nt crosslinked RNAs and upper band both 22 nt and larger RNAs (Fig. 1c). These products were sequenced with high throughput methods26 and found to correspond to miRNAs and mRNAs, respectively (Supplementary Table 1), suggesting that Ago might be sufficiently close to both miRNA and target mRNAs to form crosslinks to both molecules in the ternary complex (Fig. 1d). Such a result would allow the search for miRNA binding sites to be constrained to both the subset of miRNAs directly bound by Ago-CLIP and to the local regions of mRNAs to which Ago crosslinked, potentially reducing the rate of false positive predictions of miRNA binding.

Fig. 1
Argonaute HITS-CLIP

To differentiate robust from non-specific or transient Ago-RNA interactions, we compared the results from biologic replicate experiments done with two different monoclonal antibodies (Supplementary Figs. 1-3). The background was further reduced by in silico random CLIP, a normalization algorithm that accounted for variation in transcript length and abundance (Supplementary Figs. 4-5). The set of Ago-crosslinked miRNAs and mRNAs were highly reproducible. Among biologic triplicates or among 5 replicates done with two antibodies, the Pearson correlation coefficient was R2 > 0.9 and > 0.84, respectively, for Ago-miRNA CLIP (Fig. 1e, Supplementary Fig. 2) and R2 ≥ 0.8 and ≥ 0.65, respectively, for Ago-mRNA CLIP (Fig. 1f, Supplementary Fig. 3). We identified 454 unique miRNAs crosslinked to Ago in mouse brain, with Ago-miR-30e being the most abundant species (14% of total tags; Supplementary Fig. 2); these results were consistent with previous estimates assessed by cloning frequency32 or bead-based cytometry33, although the correlation with published results (R2 = 0.2 - 0.32; Supplementary Fig. 6) was not as high as among our biologic replicates. These discrepancies might be due to differences in the ages of brain used, regulation of Ago-mRNA interactions, and/or increased sensitivity allowed by stringent CLIP conditions and consequent improved signal:noise. To facilitate the analysis of large numbers of Ago-mRNA CLIP tags (~1.5 × 106 unique tags; Supplementary Table 1), we analyzed overlapping tags (clusters)26, which were normalized by in silico random CLIP and sorted by biologic complexity26 (“BC”; a measure of reproducibility between biologic replicates; see Supplementary Figs. 5, 7). 1,463 robust clusters (BC=5; i.e. harboring CLIP tags in all five biologic experiments using both antibodies) mapped to 829 different brain transcripts, and 990 clusters had at least 10 tags (Supplementary Fig. 7).

Ago-mRNA HITS-CLIP tags were enriched in transcribed mRNAs (Fig. 1g). The pattern of tags mirrored the results of functional assays with miRNAs34, which show no biologic activity when seed sites are present in the 5′ UTR (1% Ago-mRNA tags), and high efficacy in 3′ UTRs (40% tags including 8% within 10kb downstream of annotated transcripts, regions likely to have unannotated 3′ UTRs26,35). In addition, an extensive set of tags were identified in other locations, including coding sequence (CDS; 25%), a location for which there is emerging evidence of miRNA regulation36-40, introns (12%), and non-coding RNAs (4%), suggesting that these sites may provide new insights into miRNA biology. Within mRNAs, Ago-mRNA clusters were highly enriched in the 3′ UTR (~60%; Supplementary Fig. 8), especially around stop codons (with a peak ~50 nt downstream) and at the 3′ end of transcripts (~70 nt upstream of presumptive poly(A) sites; P<0.003, Fig. 1h), consistent with bioinformatic observations from microarray data34. Taken together, this data suggested that Ago-mRNA clusters might be associated with functional binding sites.

To examine the relationship between Ago-mRNA clusters and potential sites of miRNA action, we first performed an unbiased search for all 6-8 nt sequence motifs within Ago-mRNA clusters using linear regression analysis (Supplementary Methods). The 6 most enriched motifs corresponded to seed sequences present within the most frequently crosslinked miRNAs in Ago-miRNA CLIP (Ago-miRNAs; Supplementary Table 2). The most significant match corresponded to the seed sequence of miR-124, a well-studied brain-specific miRNA (P = 8.3 × 10-58; since miR-124 is only the 8th most frequently crosslinked Ago-miRNA, this data may indicate over-representation of miR-124 seed matches in the genome (Supplementary Table 2-3), or contributions from currently unknown rules of Ago binding). To more precisely define the region of mRNA complexed with Ago, we examined the width of 61 robust Ago-mRNA clusters (BC = 5; total tags > 30) relative to their peaks (determined by cubic spline interpolation; Supplementary Methods). We found that Ago bound within 45-62 nt of cluster peaks ≥ 95% of the time (Fig. 2a), and we defined this region as the average Ago-mRNA footprint.

Fig. 2
Distribution of mRNA tags correlates with seed sequences of miRNAs from Ago CLIP

Within Ago-mRNA footprints (11,118 clusters; BC≥2) we found a high correlation between the frequency of Ago-miRNAs and the frequency of their seed matches (Fig. 2b and Supplementary Figure 9). The seed matches were near the peaks of 134 robust clusters (Fig. 2c; Supplementary Table 3 and Supplementary Figure 13), and their distribution was leptokurtic (with a higher and sharper peak than a normal distribution; excess kurtosis (k) = 1.08, versus 0 in a normal distribution). As a control, seed matches for a “negative” group of miRNAs (the lowest ranks from the Ago-miRNA list) were uniformly distributed across these clusters (Fig. 2c; k = -1.35, versus -1.2 in uniform distribution). Taken together, these results indicate that the Ago-mRNA footprint is rich in and may predict miRNA binding sites with enhanced specificity over purely bioinformatic approaches (at least a 3-fold improvement in false-positives; Supplemental Methods).

Ago HITS-CLIP and miR-124

To further explore the relationship between the Ago-mRNA footprint and miRNA binding we focused on miR-124 sites. There was a marked enrichment of conserved miR-124 seed matches in Ago-mRNA clusters (BC ≥ 2; Fig. 2d, estimated false-positive rate of 13%; Supplementary Methods). 86% of the predicted miR-124 binding sites were present within the Ago footprint region, again in a tight peak region showing leptokurtic distribution (Fig. 2d; k = 11.63). While some predicted seeds outside of the Ago footprint might correspond to false positives, we noted small secondary peaks at ~ +/- 50 nt outside the Ago footprint (Fig. 2d), suggesting the possibility of cooperative secondary miR-124 binding sites in some transcripts, consistent with prior data34. Relative to more stringent analyses (Fig. 2C), our analysis at this threshold (BC ≥ 2) was more sensitive and sufficiently specific such that we used it for subsequent analyses (Supplementary Figure 7).

We searched published examples of miR-124 regulated transcripts for Ago-mRNA clusters harboring miR-124 seeds within the predicted 62 nt Ago footprint (referred to as Ago-miR-124 ternary clusters). We identified such ternary clusters in 5 of 5 transcripts in which miR-124 seed sites had been well defined by functional studies (including mutagenesis of seed sequences in full length 3′UTR; Fig. 4 and Supplementary Fig. 10). In each of these transcripts, there were many predicted miRNA target sites in the 3′ UTR relative to the small number of Ago-mRNA ternary clusters found, suggesting that there may be a significant number of false positive predictions from bioinformatic algorithms (Fig. 3 and Supplementary Fig. 10). For example the 3′ UTR of Itgb1 mRNA has ~50 predicted miRNA target sites, including two miR-124 sites, but only 5 Ago-mRNA ternary clusters (Fig. 3a). Using the Ago footprint to predict which miRNAs bound at these sites (Ago ternary map; Supplementary Methods) we identified three as miR-124 sites, one of which was not predicted computationally because the seed sequence is not conserved (Fig. 3a); similar observations were made in the Ctdsp1 3′ UTR (Supplementary Fig. 10). Previous luciferase assays demonstrated that miR-124 suppression of Itgb1 (to 35% control levels) was partially reversed (to 85% control levels) by mutating both of the two predicted seed sequences41; our observation of an Ago-miR-124 ternary cluster at this third non-conserved site may explain the partial rescue. Conversely, in the Ptbp1 3′ UTR, the absence of any Ago footprint at a predicted miR-9 seed site was consistent with prior studies which found this site to be non-functional42 (Fig. 3b). Additionally, Ptbp1 has seven predicted miR-124 seed sites, of which five were previously tested and only 2 found to be functional in luciferase assays42; only these 2 sites harbored Ago-miR-124 ternary clusters (Fig. 3b).

Fig. 3
Ago-miRNA ternary clusters in validated miR-124 mRNA targets
Fig. 4
Meta-analysis of Ago-mRNA clusters in large-scale screens of miR-124 regulated targets

To extend these observations, we compiled brain-expressed transcripts from a meta-analysis of five published microarray experiments which identified transcripts downregulated following miR-124 overexpression in HeLa and other cell lines (Supplementary Methods)7,11,21,42,43. Brain-expressed transcripts that had predicted 3′ UTR miR-124 seeds were also suppressed at the mRNA and protein level by miR-124 overexpression (Fig. 4), consistent with previous experiments11,18. However, transcripts with Ago-miR-124 ternary clusters had a significantly greater tendency to be downregulated at the mRNA and protein level (P<0.01, Kolmogorov-Smirnov test, Fig. 4). Ago-miR-124 ternary clusters had much greater predictive value (true positive rate = 73%) and specificity (92.5%) than analysis of conserved seed sequences alone (Supplementary Fig. 11). We validated these studies experimentally by examining Ago-mRNA clusters that appeared de novo in HeLa cells (which do not express endogenous miR-124) after miR-124 transfection. Applying this data to the meta-analysis, mRNAs whose 3′ UTRs harbored new AgomiR-124 ternary clusters after miR-124 transfection showed an even greater enrichment in miR-124-dependent changes in transcript (Fig. 4c) and protein (Fig. 4d) levels (P<0.01, Kolmogorov-Smirnov test).

We next examined Ago-miR-124 ternary clusters present in validated individual transcripts identified from among 168 candidate miR-124 regulated transcripts7. These targets were previously analyzed by Hannon and colleagues44 using a rigorous 3-part strategy to experimentally validate 22 of them (although miR-124 binding sites were not generally defined). 16 of 22 harbored Ago-miR-124 ternary clusters in the 3′ UTR (Table 1), and in 5 additional transcripts with low expression levels, Ago-mRNA CLIP-tags were identified at predicted miR-124 seed sites. For transcripts of even moderate abundance (with normalized probe intensities ≥700; average for P13 brain transcripts ~1,255; Supplementary Methods), we identified all 10/10 predicted targets (Table 1). These data indicate that identifying Ago-miR-124 ternary clusters markedly enhances the sensitivity and specificity of detecting bona fide miR-124 targets.

Table 1
Ago-miR-124 ternary maps in brain and transfected HeLa cells

We examined this set of 22 targets44 for Ago-mRNA clusters that appeared in the 3′ UTR after miR-124 transfection (Table 1). Remarkably, from among many potential miR-124 seed sites, 17 de novo Ago-miR-124 ternary clusters appeared after transfection and 14 of 17 were at precisely the positions predicted from brain Ago miR-124 maps. Genome-wide, a total of 526 of new Ago-miR-124 clusters were detected in transcripts expressed in HeLa cells and mouse brain. These results confirmed that the Ago ternary map identifies functional sites of miRNA regulation.

Predicting miRNA functional networks

Based on the robust correlation between previously validated miR-124 functional sites and Ago HITS-CLIP, we examined brain Ago-mRNA clusters to predict binding maps for the 20 most abundant Ago-miRNAs (Supplementary Method). These maps (Fig. 5a) show that Ago binds to target transcripts at very specific sites: on average there are only 2.6 Ago-mRNA clusters (BC ≥ 2) per Ago regulated transcript (2.12 per 3′ UTR) and each miRNA binds an average of only 655 targets (Supplementary Figs. 7 and 13). To explore the potential of Ago HITS-CLIP maps to define miRNA regulated transcripts, we examined the functions encoded by the predicted targets of these 20 miRNAs using gene ontology (GO) analysis; comparison of these results with predictions made using GO analysis of TargetScan predictions (Supplementary Fig. 14) demonstrates that the false discovery rate (FDR) and ‘quality’ of the protein network deteriorate substantially when the Ago-mRNA map is not used. Target predictions from the Ago HITS-CLIP map suggest that diverse neuronal functions are regulated by different sets of miRNAs (Fig. 5b). The largest set of miRNA associated functions, “neuronal differentiation” illustrates interwoven but distinct pathways predicted to be regulated by three miRNAs expressed in neurons (Fig. 5c). The Ago-RNA ternary map corresponds remarkably well with the current view of miR-124, miR-125 and miR-9 biology, including actions to promote neurite outgrowth and differentiation by inhibiting Ago-miR-124 targets (Fig. 5c; discussed in Supplemental Fig. 15).

Fig. 5
Ago-miRNA ternary maps

Discussion

Ago-miRNA-mRNA ternary maps identify functionally relevant miRNA binding sites in living tissues, and were developed in the context of several recent studies. Crystallographic structures of Ago miRNA-mRNA ternary complexes31 demonstrated close contacts between all three molecules, consistent with the ability of CLIP, which requires close protein-RNA contacts28,29, to detect both Ago miRNA and mRNA interactions. The development of HITS-CLIP set the stage for generating and analyzing genome-wide RNA-protein maps in the brain26 and cultured cells30. High throughput experiments and bioinformatic analysis together generated genome-wide predictions of miRNA seed sequences, particularly of miR-124. These studies demonstrated that miR-124 simultaneously represses hundreds of transcripts7,11,21,42-44, and provided a genome-wide “gold standard” with which to compare Ago HITS-CLIP data. This allowed estimates specificity, false positive and false negatives rates (~93%, ~13-27% and ~15-25%, respectively; Supplementary Methods) that, although limited to one Ago-miRNA-mRNA dataset, indicate that experimental Ago HITS-CLIP data outperformed bioinformatic predictions alone (Fig. 4; Supplementary Fig. 11).

Although we used seed-driven approaches to validate targets, not all Ago binding need be constrained by these rules. 27% of Ago-mRNA clusters have no predicted seed matches among the top 20 Ago-miRNAs families. Such orphan clusters might bind other miRNAs, or miRNAs that follow other rules of binding, such as wobble or bulge nucleotides40,45,46 (Chi & Darnell, unpublished observations). Orphan clusters also provide another means of estimating the false negative rate (~15%; Supplementary Methods), which compares favorably with previous studies in which false negative rates were between 50% and 70%11,18,20.

Ago HITS-CLIP resolves some roadblocks that have arisen in efforts to understand miRNA action. It has been difficult to discriminate direct from indirect actions of miRNAs, and to extrapolate miRNA overexpression studies in tissue culture to organismal miRNA action. Target RNAs have previously been identified by immunoprecipitation, microarray analysis21,44, and reporter validation assays, with the concern that low stringency immunoprecipitation of non-crosslinked RNA-protein complexes47, including Ago-miRNAs48, may purify indirect targets. This has spurred interest in efforts to explore miRNA-target identification by covalently crosslinking, using formaldehyde or 4-thio-uridine modified RNA in culture to identify transcripts complexed with Ago, miRNAs and additional proteins48,49. HITS-CLIP offers a clear means of identifying direct Ago targets and identifying specific interaction sites, which in turn offers the possibility of specifically targeting miRNA activity.

Ago-HITS-CLIP compliments bioinformatic approaches to miRNA target identification by restricting the sequence space to be analyzed to the ~45-60 nt Ago footprint. For highly conserved 3′ UTRs, such as those of the RNABPs Ptbp2, Nova1, and Fmr1, many miRNA sites are predicted using algorithms that rely on sequence conservation, but each has only one Ago-mRNA CLIP cluster (Supplementary Fig. 10). In fact, miRNA selectivity is very high (Fig. 4) such that on average transcripts have between 1-3 major Ago binding sites in a single tissue (Fig. 3 and Supplementary Figs. 10, 13-14). Ago-mRNA binding sites themselves have no apparent sequence preference (data not shown), suggesting that accessibility may rely on additional RNABPs. Such a mechanism, which may be assessed by overlaying HITS-CLIP maps of different RNABPs26, could provide a means of dynamically regulating miRNA binding and regulation1.

By simultaneously generating binding maps for multiple miRNAs, Ago HITS-CLIP offers a new approach to understanding combinatorial control of target RNA expression. Both the FDR rate and evidence for such miRNA target protein networks deteriorate substantially when predicted maps are generated without experimental Ago HITS-CLIP data (Supplementary Fig. 15). At the same time, analysis of a single miRNA, miR-124, demonstrated that its expression not only induced Ago to bind miR-124 sites, but reduced or precluded Ago binding to sites occupied in untransfected cells (Table 1), perhaps reflecting competition between a limited capacity for miRNA binding on a given 3′ UTR. Such Ago occlusion has important mechanistic, experimental and clinical implications, where studies manipulating miRNA levels are envisioned.

Methods summary

Ago HITS-CLIP was performed in biologic replicate as described26,27 (using monoclonal antibody 2A8 or 7G1-1* as described in Supplementary Methods). High-throughput sequencing was performed with an Illumina Genome Analyzer.

Microarrays. Affymetrix exon arrays (MoEx 1.0 ST) were used to measure transcript abundance in P13 mouse brain and data was analyzed with Affymetrix Power Tools.

Bioinformatics analysis used the UCSC genome browser, miRBASE, BioPython, Scipy and GoMiner, as described in Supplementary Methods.

Supplementary Material

supp data set

supp text

Acknowledgments

The authors thank members of the Darnell laboratory for discussion, J. Fak for help with exon arrays, C. Zhang for bioinformatic discussions, and G. Dunn, D. Licatalosi, C. Marney, M. Frias, T. Eom, J. Darnell, M. Yano and C. Zhang for critical review of the manuscript. Thanks to Z. Mourelatos for generously supplying the 2A8 antibody and communicating unpublished results, to G. Hannon for helpful discussions, and to Scott Dewell for help with high throughput sequencing. This work was supported in part by grants from the NIH (R.B.D.), the Cornell/Rockefeller/Sloan-Kettering Tri-Institutional Program in Computational Biology and Medicine (S.W.C.) and MD-PhD Program (J.B.Z.). R.B.D. is an Investigator of the Howard Hughes Medical Institute.

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