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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Circ Heart Fail. Author manuscript; available in PMC Aug 27, 2009.
Published in final edited form as:
PMCID: PMC2733535
NIHMSID: NIHMS130355

MRI of a Novel Murine Working Heart Transplant Model

Jose-Luiz Figueiredo, MD,1,2,* Matthias Nahrendorf, MD, PhD,1,2,* David E. Sosnovik, MD,2,3 and Ralph Weissleder, MD, PhD1,2

Heart transplant recipients require frequent invasive endomyocardial biopsies, which carry a significant risk of complication and are prone to sampling errors. Although newer immunosuppressive therapies continue to improve survival rates, organ rejection still occurs and close surveillance of the graft is mandatory. There is, thus, a compelling need to develop (1) better immunosuppressive and tolerogenic strategies and (2) noninvasive, more predictive, and quantitative diagnostic tools to monitor individual patients and to test new treatment regimens in animal models and patient cohorts. Targeted molecular imaging approaches offer the potential for more sensitive early detection of transplant rejection.14 Such emerging strategies, however, have to be validated in murine models that resemble rejection of the working human heart.

The retrogradely perfused, beating but nonworking heterotopic heart transplant model in the mouse has been extensively used in more than 30 years of research to address basic immunologic questions of rejection and tolerance because the mouse is the preferred species for basic immunology research. To our knowledge, to date, there have been no reports on working heart mouse models. The nonworking murine model has several physiologic limitations, because there is no forward flow through the grafted heart resulting from anastomosis of the donor aorta to the recipient aorta and the donor superior vena cava to the recipient inferior vena cava (IVC). Imaging studies as well as research into new immunosuppressive strategies would greatly benefit from a more realistic murine model with blood flow and cardiac workload conditions closer to human orthotopic transplantation. Orthotopic transplantation is technically challenging in the mouse, mainly because miniaturized bypass machinery is currently unavailable. We have, therefore, developed a new surgical technique of heterotopic combined heart/lung transplantation that results in a working heart, which can be imaged to assess functional parameters, such as ejection fraction and wall motion (Figure).

Figure
A, the surgical approach and direction of blood flow (arrows) are shown. B, The explanted donor heart/lung package is stored in ice-cold cardioplegic solution before implantation into the recipient. C, Surgical field after transplantation (magnification ...

The institutional subcommittee on research animal care approved all animal studies. During deep isoflurane anesthesia, we explanted donor hearts together with the right lung after ligation of the IVC and the left pulmonary arteries and veins from C57B/6 mice (Figure). In the recipient C57B/6, a median abdominal incision exposed the abdominal aorta and IVC, which were side clamped. We then performed an end-to-side anastomosis of donor aorta to recipient aorta and an end-to-side anastomosis of the donor superior vena cava to the recipient IVC (Figure D and Movie 1). After unclamping of the recipient aorta and IVC, the donor heart spontaneously resumed beating at a heart rate slightly lower than the orthotopic heart (Movie 2). We have now reached a surgical success rate of ≈90% (n=8). This surgical approach is similar to the one described by Ho and coworkers, who performed block heart and lung transplants in rats.13 These surgeries are technically difficult even in the rat (10× the body weight of the mouse), and it was unknown whether they could be adapted to murine physiology. Furthermore, in their earlier work, the superior vena cava was not anastomosed1 as in the later publications3 or in the technique described here.

Compared with the nonworking model (Movie 3), the original direction of blood flow through the heart and the lung is preserved (Figure A), and the heart is pumping blood. This has 2 major advantages: (1) the overall situation of the heterotopic graft better approximates orthotopic transplantation, and therefore, data are more translatable, and (2) because the heart is working, one can measure regional and global function with tagged and cine MRI (Movies 4 and 5) and, in the future, investigate if targeted molecular imaging approaches can detect rejection before function declines. The movies also highlight the potential of noninvasive quantitative imaging of basic transplant rejection studies. Traditionally, the only noninvasive read out is abdominal palpation of the recipient mouse, and the quality and frequency of the heart motion are described using a 4-level beating score. This index may be observer dependent and has a limited dynamic range (score 1 to 4), whereas imaging can detect subtle changes in ejection fraction or even regional myocardial dysfunction in tagging MRI observer independently.

Supplementary Material

suppl movies

Acknowledgments

We acknowledge the help of Elisabeth Zhang, BS, and Cory Siegel, MD, in the CSB Mouse imaging program.

Sources of Funding

This study was supported in part by grant R24-CA92782 (to R.W.).

Footnotes

The online-only Data Supplement is available at http://circheartfailure.ahajournals.org/cgi/content/full/2/3/272/DC1.

Disclosures

None.

References

1. Kanno S, Wu YJ, Lee PC, Dodd SJ, Williams M, Griffith BP, Ho C. Macrophage accumulation associated with rat cardiac allograft rejection detected by magnetic resonance imaging with ultrasmall superparamagnetic iron oxide particles. Circulation. 2001;104:934–938. [PubMed]
2. Wu Y, Ye Q, Foley LM, Hitchens TK, Sato K, Williams JB, Ho C. In situ labeling of immune cells with iron oxide particles: an approach to detect organ rejection by cellular MRI. Proc Natl Acad Sci USA. 2006;103:1852–1857. [PMC free article] [PubMed]
3. Ye Q, Wu YL, Foley LM, Hitchens TK, Eytan DF, Shirwan H, Ho C. Longitudinal tracking of recipient macrophages in a rat chronic cardiac allograft rejection model with noninvasive magnetic resonance imaging using micrometer-sized paramagnetic iron oxide particles. Circulation. 2008;118:149–156. [PMC free article] [PubMed]
4. Christen T, Nahrendorf M, Wildgruber M, Swirski FK, Aikawa E, Waterman P, Shimizu K, Weissleder R, Libby P. Molecular imaging of innate immune cell function in transplant rejection. Circulation. 2009 Mar 30; doi: 10.1161/CIRCULATIONAHA.108.796888. Epub ahead of print. [PMC free article] [PubMed] [Cross Ref]
5. Zhou R, Pickup S, Glickson JD, Scott CH, Ferrari VA. Assessment of global and regional myocardial function in the mouse using cine and tagged MRI. Magn Reson Med. 2003;49:760–764. [PubMed]

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