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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Science. Author manuscript; available in PMC Feb 13, 2010.
Published in final edited form as:
PMCID: PMC2729540
NIHMSID: NIHMS127610

An ABC transporter controls export of a Drosophila germ cell attractant

Directed cell migration, which is critical for embryonic development, leukocyte trafficking and cell metastasis, depends on chemoattraction. HMG-CoA reductase regulates the production of an attractant for Drosophila germ cells that may itself be geranylated. Chemoattractants are commonly secreted through a classical, signal peptide-dependent pathway, but a geranyl-modified attractant would require an alternative pathway. In budding yeast, pheromones produced by a-cells are farnesylated and secreted in a signal peptide-independent manner, requiring the ABC transporter Ste6p. Here, we show that germ cell migration uses a similar pathway, demonstrating that germ cells, like yeast, are attracted to lipid-modified peptides. Components of this unconventional export pathway are highly conserved, suggesting that this pathway may control the production of similarly modified chemoattractants from yeast to man.

In most organisms germ cells migrate from their site of origin to the somatic part of the gonad where they develop into egg and sperm. In Drosophila, germ cells migrate as single cells in a stereotyped manner and are guided by repellent and attractive cues toward the somatic gonad in the mesoderm (1). HMG-CoAr (3-hydroxyl-3-methyl-glutaryl-CoA reductase or Hmgcr) activity controls germ cell attraction to the mesoderm and recruitment of germ cells to the somatic gonad. In hmgcr mutant embryos germ cells fail to reach the somatic gonad; moreover, ectopic Hmgcr expression is sufficient to attract germ cells to a new location (2). Embryos mutant for several enzymes in the hmgcr pathway, including the β subunit of type I geranygeranyl transferase, βGGTI, are similarly defective in germ cell migration, suggesting that geranylation is critical in attracting germ cells to the mesoderm (3).

Because hmgcr or βggtI mutant embryos show a rather specific germ cell migration defect, we favored the idea that the Hmgcr pathway was required to geranylate a critical germ cell attractant rather than regulating a pathway that controlled synthesis or secretion of the attractant (3). This idea posits secretion of a geranyl modified germ cell attractant, which would preclude secretion by a classic signal-peptide dependent secretory pathway and require an alternative export mechanism. Such a mechanism has been described in yeast, where ABC transporters export farnesylated pheromones required for cell mating (4, 5). We therefore asked whether a similar export mechanism exists in Drosophila and is required for germ cell attraction.

ABC transporters are conserved from bacteria to human and shuttle hydrophobic, lipophilic compounds in an ATP dependent manner (6, 7). In S. cerevisiae and S. pombe, the ABCB family members Ste6 and Mam1 play essential roles in the export of farnesyl-modified a-type and M-type mating factors, respectively. The human ABCB family member mdr1 (multi-drug-resistant) gene is amplified in multi-drug resistant cells and its homolog mdr3 is a functional homolog of STE6 (8, 9), suggesting a close relationship between the ability of this class of transporters to export drugs and lipid modified signaling molecules. To determine whether ABCB transporters have a role in exporting the putative Drosophila germ cell attractant, we analyzed expression patterns and germ cell migration in embryos mutant for ABCB transporters (7) (Table S1). Among these, only mdr49 showed an expression pattern and mutant phenotype consistent with a role in germ cell migration (Fig 1A, Fig S1, Table S2). We generated a strong loss of function allele, mdr49δ3.16, (Fig 1B, Fig S1A, Table S2), and embryos mutant for mdr49δ3.16 showed defects in germ cell migration, like mutants in the Hmgcr pathway: germ cells migrated through the posterior midgut but then failed to associate with the somatic gonad (Fig 1C), which was properly specified (Fig S1E). This migration phenotype was only observed in mdr49 mutants and not in mutants for other ABCB transporters, such as Mdr50, Mdr65 and CG7955 (Table S1). To determine whether mdr49 function is required in the mesoderm we restored mdr49 expression selectively in the mesoderm (Fig. S1F) and found that it fully rescued the mdr49 mutant phenotype (Fig. 1D). To test whether Mdr49 acts as an ABCB transporter we asked whether Ste6p rescued the germ cell migration phenotype observed in mdr49 embryos. Expressing STE6 in the mesoderm rescued the migration defect (Figs. 1D, S1F), suggesting that Mdr49 is functionally equivalent to Ste6p and acts in mesodermal cells to attract germ cells.

Figure 1
The ABC transporter mdr49 is required for germ cell migration to the mesoderm. (A) in situ hybridization to wild-type embryos shows mdr49 mRNA expression (purple) specifically in mesodermal tissues (B) RT-PCR of mdr49δ3.16 allele (3), compared ...

The germ cell migration phenotypes caused by mutations in Mdr49 and in components of the Hmgcr pathway, such as Geranylgeranyl-Diphosphate Synthase and βGGTI, are strikingly similar (2, 3). Thus, to determine whether Mdr49 acts as a transporter for a germ cell attractant that is geranyl-geranyl modified by the Hmgcr pathway, we tested the genetic epistasis between hmgcr and mdr49. Previous experiments showed that over-expression of hmgcr in the central nervous system (CNS) is sufficient to attract germ cells to this tissue (2). We therefore reasoned that Mdr49 function should be necessary for the export of the ectopically produced attractant and that mutations in mdr49 should suppress the hmgcr misexpression phenotype. Indeed, germ cell migration to the CNS was suppressed by reducing mdr49 copy number using either the mdr49 deficiency or P-element mutation. (Fig 2 and Table S3). Mutations in other ABCB transporters, such as mdr50, mdr65 and CG7955 did not significantly suppress the hmgcr overexpression phenotype (Table S1), consistent with a specific role for Mdr49 as an exporter for an Hmgcr-dependent geranyl-modified germ cell attractant.

Figure 2
Hmgcr dependent germ cell migration to the CNS is suppressed by reducing mdr49 activity. All embryos are stage 13. (a) In more than 50% of embryos overexpressing hmgcr in the CNS >5 germ cells (brown) migrate to CNS (between lines). Ectopic migration ...

Prenylated proteins require additional modifications to be fully functional: cleavage of the AAX residues, prenyl proteolysis, and carboxymethylation (10) (Fig 3A). We therefore asked whether the enzymes that catalyze these reactions are found in Drosophila and are required for germ cell migration. Prenyl proteolysis is catalyzed by Ste24p (prenyl protease type I) and Rce1p (prenyl protease type II), with the former being essential for a-factor modification (11, 12). Carboxymethylation is achieved exclusively through the activity of Ste14p (13). Orthologues of these enzymes are also found in mammals where they have similar substrates (Table S4). A genomic search in Drosophila revealed predicted orthologs for: (1) prenyl protease type I as a cluster of genes CG9000/CG9001/CG9002, which we term Dmel\Ste24, (2) prenyl protease type II termed Sras, and (3) the isoprenylcysteine carboxylmethyltransferase as CG11268, termed Dmel\Ste14 (Table S4, Fig S2).

Figure 3
Prenyl proteases Type I and isoprenylcysteine carboxymethyltransferases are required for germ cell migration to the mesoderm.

We next assessed the role of each enzyme in germ cell migration. Since specific mutations were not available for these enzymes we analyzed deficiencies that deleted each gene (as well as other genes (14)). Embryos homozygous mutant for Df(2R)Dmel\ste24, which deletes the three Drosophila ste24 genes, showed a specific germ cell migration phenotype similar to that of embryos mutant for mdr49. On average, six germ cells did not associate with the somatic gonad (Fig 3B and Table S2). Next, we asked whether the single Drosophila isoprenylcysteine carboxylmethyltransferase, Dmel\ste14, is required for germ cell migration. We were unable to analyze the germ cell migration phenotype of embryos homozygous for the deletion Df(3L)ED4486 (Df(3L)Dmel\ste14) due to embryonic patterning defects, which resembled those observed in mutants for Drosophila Rho GTPases and gave additional evidence that Dmel\ste14 encodes a general post-prenylation processing enzyme that modifies all prenylated proteins (1517) (Fig S4). Instead of analyzing homozygous mutants we asked whether reducing Dmel\ste14 gene dosage was able to suppress germ cell mis-migration induced by ectopic expression of hmgcr in the CNS. As observed for the ABC transporter mdr49, reducing Dmel\ste14 gene dosage significantly suppressed Hmgcr-dependent ectopic germ cell migration, suggesting a requirement of this enzyme in attractant modification (Fig 3C, Table S5).

Our genetic analyses strongly suggest that a geranylgeranylated germ cell attractant is produced and modified in the somatic gonad and exported via an ABCB transporter. In order to more directly test for the production of a molecule that can act as a diffusible attractant, we devised an in vitro germ cell migration assay. We used FACS-sorted germ cells from 2–10 hour old embryos that expressed Moesin-GFP specifically in germ cells (18). Germ cells were placed in the upper well of a transwell chemotaxis chamber and scored for their migration toward Kc cell-conditioned medium (19), in the lower well (Fig. 4A). Germ cells migrated to the bottom well containing conditioned medium from cells overexpressing hmgcr and mdr49 (Fig. 4B). Fewer cells moved toward unconditioned control medium or medium conditioned from parental Kc cells, which express low levels of hmgcr and mdr49 (19) (Fig S5A). To determine whether migration toward parental Kc cells is dependent upon hmgcr and mdr49 we used RNA interference (RNAi) to reduce the expression of Hmgcr pathway members in Kc cells. We found that reducing hmgcr, gerany-geranyl transferase 1 (βGGTI) and ABC transporter (mdr49) expression fully blocked germ cell migration toward Kc cell-conditioned medium (Fig 4C, Fig S5B). These results are consistent with our genetic data and support the notion that the prenylated Drosophila germ cell attractant is active in a secreted form.

Figure 4
Germ cells migrate towards a diffusible attractant. (A) Scheme of transwell migration assay procedure. (a) The box represents the germ cell population collected (b, c) FACS sorted germ cell population is homogenous and expresses germ cell markers: germ ...

To test whether the classic, signal-peptide dependent pathway is also required for secretion of the germ cell attractant, we used RNAi to reduce the levels of syntaxin 5, which encodes a SNARE essential for constitutive secretion (20). Germ cells were similarly attracted to conditioned medium from parental cells and syntaxin 5 deficient KC cells, indicating that production of the attractant does not depend upon constitutive secretion (Figs. 4C, S5A). Our results demonstrate that the Drosophila germ cell attractant is geranylgeranylated and secreted by mesodermal cells in a signal peptide-independent manner through an ABCB transporter. The modifications, processing and export pathway used to generate and secrete the germ cell attractant strikingly resemble that of a-factor in mating yeast.

ABC transporters have been studied mostly in the context of cancer drug resistance or their role in toxin protection. Our findings reveal a new function for this conserved, non-conventional secretory pathway in a multicellular organism and suggest that this pathway may be used to export signals required for cell-cell communication in organisms other than Drosophila and yeast (2123). Yeast lacks many of the secreted cell signaling molecules such as Hh, Wnt, and BMP used by multicellular organisms to communicate between cells. The use of a prenylated signal may thus be an ancient mechanism of cell communication. It is striking that this pathway is used in yeast and flies to facilitate the migration and adhesion of germ cells, the essential cells for reproduction.

Supplementary Material

Acknowledgments

We thank Peter Lopez and Gelo de la Cruz for FACS sorting, Susan Michaelis for reagents and discussions, Jessica Treisman, Blomington stock center and the DGRC for fly lines and reagents, the Lehmann Lab, Daria Siekhaus and Steve Burden for comments on the manuscript. This work was supported by NIH grant HD49100, S.R. is a HHMI research associate; R.L is an HHMI investigator and a member of the Kimmel Stem Cell Center.

Footnotes

Sentence summarizing most important point of paper: A HMGCoA reductase-based, secreted germ cell chemoattractant in Drosophila uses a non-conventional ABCB transporter system for export; conservation of the processing and export machinery from yeast to man suggests widespread use of such chemoattractants.

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