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J Clin Microbiol. Sep 1982; 16(3): 584–586.
PMCID: PMC272420

Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids.


Analysis of fatty acid methyl esters prepared from whole-cell bacteria by sodium hydroxide hydrolysis and boron trichloride-catalyzed methylation showed degradation of hydroxy acid peaks after several injections on a fused silica capillary column. A simple base wash of the extracts before injection prevented the tailing of the hydroxy acid peaks even after extended use. This eliminates the need to form trifluoroacetic anhydride derivatives of the hydroxy acids.

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Selected References

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  • Dees SB, Moss CW. Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens. J Clin Microbiol. 1975 May;1(5):414–419. [PMC free article] [PubMed]
  • Moss CW. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr. 1981 Jan 9;203:337–347. [PubMed]
  • Moss CW, Dees SB, Guerrant GO. Gas-liquid chromatography of bacterial fatty acids with a fused-silica capillary column. J Clin Microbiol. 1980 Jul;12(1):127–130. [PMC free article] [PubMed]
  • Moss CW, Nunez-Montiel OL. Analysis of short-chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column. J Clin Microbiol. 1982 Feb;15(2):308–311. [PMC free article] [PubMed]
  • Moss CW, Samuels SB, Liddle J, McKinney RM. Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia. J Bacteriol. 1973 Jun;114(3):1018–1024. [PMC free article] [PubMed]

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