• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Jun 1982; 15(6): 1167–1168.
PMCID: PMC272275

Rapid detection in spinal fluid of beta-lactamase produced by ampicillin-resistant Haemophilus influenzae.

Abstract

The chromogenic cephalosporin nitrocefin was used to detect the presence of beta-lactamase in cerebrospinal fluid of patients suffering from ampicillin-resistant Haemophilus influenzae meningitis. Five samples of spinal fluid containing ampicillin-resistant isolates were studied, and all had beta-lactamase activity. When samples of spinal fluid containing 33 ampicillin-sensitive isolates and 234 sterile specimens were tested, no beta-lactamase activity was detected.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (263K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed]
  • Catlin BW. Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance. Antimicrob Agents Chemother. 1975 Mar;7(3):265–270. [PMC free article] [PubMed]
  • Jorgensen JH, Alexander GA. Comparative activities of selected beta-lactam antibiotics against Haemophilus influenzae. Antimicrob Agents Chemother. 1978 Feb;13(2):342–343. [PMC free article] [PubMed]
  • Lee WS, Komarmy L. Iodometric spot test for detection of beta-lactamase in Haemophilus influenzae. J Clin Microbiol. 1981 Jan;13(1):224–225. [PMC free article] [PubMed]
  • Markowitz SM. Isolation of an ampicillin-resistant, non-beta-lactamase-producing strain of Haemophilus influenzae. Antimicrob Agents Chemother. 1980 Jan;17(1):80–83. [PMC free article] [PubMed]
  • Montgomery K, Raymundo L, Jr, Drew WL. Chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria. J Clin Microbiol. 1979 Feb;9(2):205–207. [PMC free article] [PubMed]
  • O'Callaghan CH, Morris A, Kirby SM, Shingler AH. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. [PMC free article] [PubMed]
  • Yolken RH, Hughes WT. Rapid diagnosis of infections caused by beta-lactamase-producing bacteria by means of an enzyme radioisotopic assay. J Pediatr. 1980 Nov;97(5):715–720. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...