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J Clin Microbiol. Dec 1985; 22(6): 996–1006.
PMCID: PMC271866

Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.


The adherence of coagulase-negative staphylococci to smooth surfaces was assayed by measuring the optical densities of stained bacterial films adherent to the floors of plastic tissue culture plates. The optical densities correlated with the weight of the adherent bacterial film (r = 0.906; P less than 0.01). The measurements also agreed with visual assessments of bacterial adherence to culture tubes, microtiter plates, and tissue culture plates. Selected clinical strains were passed through a mouse model for foreign body infections and a rat model for catheter-induced endocarditis. The adherence measurements of animal passed strains remained the same as those of the laboratory-maintained parent strain. Spectrophotometric classification of coagulase-negative staphylococci into nonadherent and adherent categories according to these measurements had a sensitivity, specificity, and accuracy of 90.6, 80.8, and 88.4%, respectively. We examined a previously described collection of 127 strains of coagulase-negative staphylococci isolated from an outbreak of intravascular catheter-associated sepsis; strains associated with sepsis were more adherent than blood culture contaminants and cutaneous strains (P less than 0.001). We also examined a collection of 84 strains isolated from pediatric patients with cerebrospinal fluid (CSF) shunts; once again, pathogenic strains were more adherent than were CSF contaminants (P less than 0.01). Finally, we measured the adherence of seven endocarditis strains. As opposed to strains associated with intravascular catheters and CSF shunts, endocarditis strains were less adherent than were saprophytic strains of coagulase-negative staphylococci. The optical densities of bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.

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Selected References

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  • Baddour LM, Christensen GD, Hester MG, Bisno AL. Production of experimental endocarditis by coagulase-negative staphylococci: variability in species virulence. J Infect Dis. 1984 Nov;150(5):721–727. [PubMed]
  • Bayston R, Penny SR. Excessive production of mucoid substance in staphylococcus SIIA: a possible factor in colonisation of Holter shunts. Dev Med Child Neurol Suppl. 1972;27:25–28. [PubMed]
  • Christensen GD, Bisno AL, Parisi JT, McLaughlin B, Hester MG, Luther RW. Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis. Ann Intern Med. 1982 Jan;96(1):1–10. [PubMed]
  • Christensen GD, Parisi JT, Bisno AL, Simpson WA, Beachey EH. Characterization of clinically significant strains of coagulase-negative staphylococci. J Clin Microbiol. 1983 Aug;18(2):258–269. [PMC free article] [PubMed]
  • Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982 Jul;37(1):318–326. [PMC free article] [PubMed]
  • Christensen GD, Simpson WA, Bisno AL, Beachey EH. Experimental foreign body infections in mice challenged with slime-producing Staphylococcus epidermidis. Infect Immun. 1983 Apr;40(1):407–410. [PMC free article] [PubMed]
  • Fletcher M. The effects of proteins on bacterial attachment to polystyrene. J Gen Microbiol. 1976 Jun;94(2):400–404. [PubMed]
  • Franson TR, Sheth NK, Rose HD, Sohnle PG. Scanning electron microscopy of bacteria adherent to intravascular catheters. J Clin Microbiol. 1984 Sep;20(3):500–505. [PMC free article] [PubMed]
  • Hogt AH, Dankert J, de Vries JA, Feijen J. Adhesion of coagulase-negative staphylococci to biomaterials. J Gen Microbiol. 1983 Sep;129(9):2959–2968. [PubMed]
  • Kloos WE. Natural populations of the genus Staphylococcus. Annu Rev Microbiol. 1980;34:559–592. [PubMed]
  • Locci R, Peters G, Pulverer G. Microbial colonization of prosthetic devices. I. Microtopographical characteristics of intravenous catheters as detected by scanning electron microscopy. Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(5):285–292. [PubMed]
  • Locci R, Peters G, Pulverer G. Microbial colonization of prosthetic devices. III. Adhesion of staphylococci to lumina of intravenous catheters perfused with bacterial suspensions. Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(5):300–307. [PubMed]
  • Lowy FD, Hammer SM. Staphylococcus epidermidis infections. Ann Intern Med. 1983 Dec;99(6):834–839. [PubMed]
  • Ludwicka A, Jansen B, Wadström T, Pulverer G. Attachment of staphylococci to various synthetic polymers. Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Apr;256(4):479–489. [PubMed]
  • Maki DG, Weise CE, Sarafin HW. A semiquantitative culture method for identifying intravenous-catheter-related infection. N Engl J Med. 1977 Jun 9;296(23):1305–1309. [PubMed]
  • Marrie TJ, Costerton JW. Scanning and transmission electron microscopy of in situ bacterial colonization of intravenous and intraarterial catheters. J Clin Microbiol. 1984 May;19(5):687–693. [PMC free article] [PubMed]
  • Marrie TJ, Costerton JW. Morphology of bacterial attachment to cardiac pacemaker leads and power packs. J Clin Microbiol. 1984 Jun;19(6):911–914. [PMC free article] [PubMed]
  • Marrie TJ, Noble MA, Costerton JW. Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy. J Clin Microbiol. 1983 Dec;18(6):1388–1398. [PMC free article] [PubMed]
  • Needham CA, Stempsey W. Incidence, adherence, and antibiotic resistance of coagulase-negative Staphylococcus species causing human disease. Diagn Microbiol Infect Dis. 1984 Sep;2(4):293–299. [PubMed]
  • Peters G, Locci R, Pulverer G. Microbial colonization of prosthetic devices. II. Scanning electron microscopy of naturally infected intravenous catheters. Zentralbl Bakteriol Mikrobiol Hyg B. 1981;173(5):293–299. [PubMed]
  • Peters G, Locci R, Pulverer G. Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. J Infect Dis. 1982 Oct;146(4):479–482. [PubMed]
  • Peters G, Saborowski F, Locci R, Pulverer G. Investigations on staphylococcal infection of transvenous endocardial pacemaker electrodes. Am Heart J. 1984 Aug;108(2):359–365. [PubMed]
  • Sheth NK, Rose HD, Franson TR, Buckmire FL, Sohnle PG. In vitro quantitative adherence of bacteria to intravascular catheters. J Surg Res. 1983 Mar;34(3):213–218. [PubMed]
  • Staat RH, Langley SD, Doyle RJ. Streptococcus mutans adherence: presumptive evidence for protein-mediated attachment followed by glucan-dependent cellular accumulation. Infect Immun. 1980 Feb;27(2):675–681. [PMC free article] [PubMed]

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