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J Clin Microbiol. Apr 1985; 21(4): 546–552.
PMCID: PMC271716

Immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals.

Abstract

The virus specificity of antibodies against bovine herpes virus type 1 was determined with a radioimmunoprecipitation assay and serum collected from natural and experimentally induced infections. By using sequentially collected sera, the development of antibodies to 4 to 5 viral glycoproteins and 11 to 12 nonglycosylated proteins was followed for the first 50 days after infection. The major and most consistent responses in experimentally and naturally infected animals were to four glycoproteins with molecular weights of 102,000, 96,000, 69,000, and 55,000, as well as to a major virion 115,000-molecular-weight nonglycosylated protein. The four glycoproteins were all coprecipitated by a neutralizing monoclonal antibody and were probably involved as target antigens in virus neutralization. Another antigenically unrelated glycoprotein with a molecular weight of 82,000 and a nonglycosylated protein with a molecular weight of 91,000 were also precipitated, but the immune response to these two proteins was transient. Reactivity to gp82 was only weakly detected in serum from naturally infected animals. Contact control animals which did not contract a bovine herpes virus type 1 infection but were exposed to infected animals with signs of severe illness had antibodies which recognized gp102, gp96, gp69 and gp55 as well as p115. These antibodies were present in low amounts and, in contrast to infected animals, did not increase between acute and convalescent sampling.

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Selected References

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  • Babiuk LA, Wardley RC, Rouse BT. Defense mechanisms against bovine herpesvirus: relationship of virus-host cell events to susceptibility to antibody-complement cell lysis. Infect Immun. 1975 Nov;12(5):958–963. [PMC free article] [PubMed]
  • Bolton DC, Zee YC, Ardans AA. Identification of envelope and nucleocapsid proteins of infectious bovine rhinotracheitis virus by SDS-polyacrylamide gel electrophoresis. Vet Microbiol. 1983 Feb;8(1):57–68. [PubMed]
  • Carter VC, Rice PL, Tevethia SS. Intratypic and intertypic specificity of lymphocytes involved in the recognition of herpes simplex virus glycoproteins. Infect Immun. 1982 Jul;37(1):116–126. [PMC free article] [PubMed]
  • Collins JK, Butcher AC, Riegel CA, McGrane V, Blair CD, Teramoto YA, Winston S. Neutralizing determinants defined by monoclonal antibodies on polypeptides specified by bovine herpesvirus 1. J Virol. 1984 Nov;52(2):403–409. [PMC free article] [PubMed]
  • Davies DH, Carmichael LE. Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. Infect Immun. 1973 Oct;8(4):510–518. [PMC free article] [PubMed]
  • Eberle R, Russell RG, Rouse BT. Cell-mediated immunity to herpes simplex virus: recognition of type-specific and type-common surface antigens by cytotoxic T cell populations. Infect Immun. 1981 Dec;34(3):795–803. [PMC free article] [PubMed]
  • Gerber JD, Marron AE, Kucera CJ. Local and systemic cellular and antibody immune responses of cattle to infectious bovine rhinotracheitis virus vaccines administered intranassally or intramuscularly. Am J Vet Res. 1978 May;39(5):753–760. [PubMed]
  • Goudswaard J, van der Donk JA, Noordzij A, van Dam RH, Vaerman JP. Protein A reactivity of various mammalian immunoglobulins. Scand J Immunol. 1978;8(1):21–28. [PubMed]
  • House JA, Baker JA. Bovine herpesvirus IBR-IPV. The antibody virus neutralization reaction. Cornell Vet. 1971 Apr;61(2):320–335. [PubMed]
  • Jericho KW, Babiuk LA. The effect of dose, route and virulence of bovine herpesvirus 1 vaccine on experimental respiratory disease in cattle. Can J Comp Med. 1983 Apr;47(2):133–139. [PMC free article] [PubMed]
  • Kahrs RF. Infectious bovine rhinotracheitis: a review and update. J Am Vet Med Assoc. 1977 Nov 15;171(10):1055–1064. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Long D, Madara TJ, Ponce de Leon M, Cohen GH, Montgomery PC, Eisenberg RJ. Glycoprotein D protects mice against lethal challenge with herpes simplex virus types 1 and 2. Infect Immun. 1984 Feb;43(2):761–764. [PMC free article] [PubMed]
  • Lupton HW, Reed DE. Evaluation of experimental subunit vaccines for infectious bovine rhinotracheitis. Am J Vet Res. 1980 Mar;41(3):383–390. [PubMed]
  • Misra V, Blumenthal RM, Babiuk LA. Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). J Virol. 1981 Nov;40(2):367–378. [PMC free article] [PubMed]
  • Misra V, Gilchrist JE, Weinmaster G, Qualtiere L, Van den Hurk S, Babiuk LA. Herpesvirus-induced "early" glycoprotein: characterization and possible role in immune cytolysis. J Virol. 1982 Sep;43(3):1046–1054. [PMC free article] [PubMed]
  • Norrild B. Immunochemistry of herpes simplex virus glycoproteins. Curr Top Microbiol Immunol. 1980;90:67–106. [PubMed]
  • Norrild B, Shore SL, Nahmias AJ. Herpes simplex virus glycoproteins: participation of individual herpes simplex virus type 1 glycoprotein antigens in immunocytolysis and their correlation with previously identified glycopolypeptides. J Virol. 1979 Dec;32(3):741–748. [PMC free article] [PubMed]
  • Rouse BT, Babiuk LA. The direct antiviral cytotoxicity by bovine lymphocytes is not restricted by genetic incompatibility of lymphocytes and target cells. J Immunol. 1977 Feb;118(2):618–624. [PubMed]
  • Rouse BT, Babiuk LA. Mechanisms of recovery from Herpesvirus infections -a review. Can J Comp Med. 1978 Oct;42(4):414–427. [PMC free article] [PubMed]
  • Savan M, Angulo AB, Derbyshire JB. Interferon, antibody responses and protection induced by an intranasal infectious bovine rhinotracheitis vaccine. Can Vet J. 1979 Aug;20(8):207–210. [PMC free article] [PubMed]
  • Wardley RC, Rouse BT, Babiuk LA. Antibody dependent cytotoxicity mediated by neutrophils: a possible mechanism of antiviral defense. J Reticuloendothel Soc. 1976 May;19(5):323–332. [PubMed]

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