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J Exp Med. May 11, 2009; 206(5): 981–989.
PMCID: PMC2715030

TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma

Abstract

Proliferation and survival of Hodgkin and Reed/Sternberg (HRS) cells, the malignant cells of classical Hodgkin lymphoma (cHL), are dependent on constitutive activation of nuclear factor κB (NF-κB). NF-κB activation through various stimuli is negatively regulated by the zinc finger protein A20. To determine whether A20 contributes to the pathogenesis of cHL, we sequenced TNFAIP3, encoding A20, in HL cell lines and laser-microdissected HRS cells from cHL biopsies. We detected somatic mutations in 16 out of 36 cHLs (44%), including missense mutations in 2 out of 16 Epstein-Barr virus–positive (EBV+) cHLs and a missense mutation, nonsense mutations, and frameshift-causing insertions or deletions in 14 out of 20 EBV cHLs. In most mutated cases, both TNFAIP3 alleles were inactivated, including frequent chromosomal deletions of TNFAIP3. Reconstitution of wild-type TNFAIP3 in A20-deficient cHL cell lines revealed a significant decrease in transcripts of selected NF-κB target genes and caused cytotoxicity. Extending the mutation analysis to primary mediastinal B cell lymphoma (PMBL), another lymphoma with constitutive NF-κB activity, revealed destructive mutations in 5 out of 14 PMBLs (36%). This report identifies TNFAIP3 (A20), a key regulator of NF-κB activity, as a novel tumor suppressor gene in cHL and PMBL. The significantly higher frequency of TNFAIP3 mutations in EBV than EBV+ cHL suggests complementing functions of TNFAIP3 inactivation and EBV infection in cHL pathogenesis.

Classical Hodgkin lymphoma (cHL) is one of the most common malignant lymphomas. It is characterized by the presence of rare Hodgkin and Reed/Sternberg (HRS) cells embedded in an extensive inflammatory infiltrate. Constitutive activation of NF-κB in HRS cells that transcriptionally regulates expression of multiple antiapoptotic factors and proinflammatory cytokines plays a central role in the pathogenesis of cHL (1, 2). In a nonstimulated condition, NF-κB proteins are rendered inactive by binding to inhibitors of NF-κB (IκBs), which sequester them in the cytoplasm. Stimulation of multiple receptors activates the IκB kinase (IKK) complex that phosphorylates IκB at two specific serine residues, followed by its ubiquitination and proteasomal degradation, thereby releasing NF-κB proteins and allowing their nuclear translocation (3). Recently, two studies provided further insights into the molecular mechanisms of IKK activation upon TNF stimulation (4, 5). Activation of the IKK complex and subsequent NF-κB activation requires Lys63 polyubiquitination of RIP1, a kinase that is recruited to the receptor upon TNF stimulation. IKK-γ (NF-κB essential modulator), the regulatory subunit of the IKK complex, specifically recognizes these Lys63-linked polyubiquitins attached to RIP1 and thereby activates IKK and NF-κB (4, 5).

A20 is a ubiquitin-modifying enzyme that inhibits NF-κB activation in succession of TNF receptor– and Toll-like receptor–induced signals (68). This enzyme removes Lys63-linked ubiquitin chains from RIP1 and adds Lys48 polyubiquitins to RIP1, thereby targeting this factor for proteasomal degradation, thus explaining the molecular mechanism of NF-κB inhibition by A20 (6). A20 also likely inhibits NF-κB activity by additional means, including interaction with TRAF1 and TRAF2 (9).

The TNFAIP3 gene, encoding A20, is located in chromosome band 6q23, a region that is frequently deleted in B cell lymphomas (10, 11). Recently, studies applying high-resolution, genome-wide cytogenetic techniques such as array-based comparative genomic hybridization (aCGH) or single nucleotide polymorphism (SNP) chip analysis on non-Hodgkin lymphoma and cHL reported a region of minimal common loss at 6q23, including TNFAIP3 (1215). However, mutations in this gene have not been reported in these studies (1215).

To test whether mutational inactivation of A20 contributes to the pathogenesis of cHL and primary mediastinal B cell lymphoma (PMBL), another lymphoma with constitutive NF-κB activity (16), we sequenced TNFAIP3 in these lymphomas, and performed functional studies with cHL cell lines.

RESULTS AND DISCUSSION

Lack of A20 in consequence of mutations in TNFAIP3 in cHL cell lines

Because the underlying mechanisms of constitutive NF-κB activity in HL and PMBL are only partly understood (17), we analyzed the A20 protein by Western blotting in HL and PMBL cell lines (Fig. 1 A). Although TNFAIP3, a direct NF-κB target gene, was reported to be strongly expressed on a transcriptional level in virtually all HL cases (18), no A20 protein was detected in four out of seven HL cell lines analyzed, as well as the PMBL cell line Karpas-1106P. DNA sequence analysis of the entire coding region of TNFAIP3 revealed a nonsense mutation, a duplication, and deletions in the A20 protein–negative HL cell lines (Table I). Only the mutated alleles were identified, explaining the absence of detectable protein in the respective cell lines. In accordance with these findings, an SNP chip analysis in L-1236, HDLM-2, and U-HO1 showed loss of heterozygosity (LOH) in 6q23, including the TNFAIP3 locus (Fig. S1). A homozygous deletion in the coding sequence of TNFAIP3 in cell line KM-H2 was previously reported (19). Because the DEV cell line originates from nodular lymphocyte–predominant HL, it was excluded from further analysis.

Figure 1.
Inactivation of A20 in cHL and PMBL. (A) Mutations in TNFAIP3 correlate with the absence of detectable A20 protein (~70 kD) in lymphoma cell lines. Immunoblotting using anti-A20 antibody was performed with each 100 µg of whole-cell extracts ...
Table I.
Sequence and gene copy number analysis of TNFAIP3 from cHL cell lines and primary HRS cells

Inactivating mutation in TNFAIP3 in primary HRS cells of EBV cHL

Extending the study to primary biopsies of 30 cHLs, we individually laser-microdissected CD30+ HRS cells, pooled 10–20 cells, and sequenced DNA of the entire coding sequence of TNFAIP3 after two rounds of seminested amplification. In cases harboring mutations, single HRS and nonneoplastic cells were additionally analyzed to confirm clonality and somatic origin of the mutations identified. Chromosomal deletions of TNFAIP3 were investigated by interphase cytogenetics (i.e., fluorescence in situ hybridization [FISH] or the combined fluorescence immunophenotyping and interphase cytogenetics [FICTION] technique; Table I, Fig. 2, and Table S1). We ascertained somatic, clonal mutations in 12 out of 30 cHL cases analyzed. Additionally, 1 out of the 30 cases showed a sequence variation that was also present in the respective nontumor cells and, hence, presumably represents a polymorphism (Table I and Fig. S2). Including the cell lines, 11 out of the 16 mutated cases showed deletion of the other allele of TNFAIP3. Mutation frequency and mutation patterns differed significantly (P = 0.002) according to the EBV status of the cHL: among 16 EBV+ cHLs, only 2 cases (12.5%) each harbored a single somatic missense mutation in the last exon of TNFAIP3, whereas 14 out of 20 EBV cHLs showed clonal mutations (70%; Fig. 1 B). In 13 out of these 14 mutated EBV cHLs, we detected nonsense mutations and insertions or deletions causing frameshifts. In 9 out of the 14 cases carrying mutations generating premature stop codons, both gene copies of TNFAIP3 were genetically inactivated as the second alleles of the gene were deleted (Table I, Fig. 1 C, and Fig. S2). We analyzed 21 additional cHLs by interphase cytogenetics for losses in 6q23 and detected signal constellations indicating deletions of TNFAIP3 in 9 cases, confirming the high incidence of deletions of the chromosomal locus of TNFAIP3 in cHL (Table S1). This is also supported by a recent aCGH study of HRS cell–rich cHL cases revealing monoallelic losses of the TNFAIP3 locus in 6 out of 10 cases (15).

Figure 2.
Chromosomal deletion of TNFAIP3 in cHL detected by interphase cytogenetics. FICTION analyses of representative cHL cases combining CD30 expression (red) and FISH probes for TNFAIP3 and chromosome 6 centromere (blue [b]). In the double-color assays in ...

Inactivating mutation in TNFAIP3 in PMBL

Global gene expression profiling revealed remarkable similarities between the transcriptional profiles of PMBL and cHL (16). Furthermore, survival and proliferation of tumor cells of PMBL also depend on constitutive NF-κB activity (16, 20). Hence, we expanded the sequence analysis on primary biopsies of PMBL and the PMBL cell line Karpas-1106P, shown to be A20 protein negative (Fig. 1 A). TNFAIP3 was sequenced after one round of amplification from whole-tissue DNA. We identified mutations in 5 out of 14 cases (36%), with each mutated case carrying at least one mutation leading to A20 inactivation (Fig. 1 B). In three primary cases in which nontumor cells could be isolated by microdissection, the somatic origin of the mutations was confirmed. In cases with more than one mutation (PMBLs 2 and 3), TNFAIP3 amplicons were further cloned to identify allelic distribution of mutations, revealing bialleic distribution of the mutations in at least one of the two cases (Table II and Fig. S2). Remarkably, deletions in 6q23.3–6q24.1 have recently been identified by aCGH in ~30% of PMBLs (21, 22).

Table II.
Sequence analysis of TNFAIP3 from primary PMBLs and cell line Karpas-1106P

Collectively, we identified 16 out of 36 cHLs and 5 out of 14 PMBLs harboring somatic mutations in TNFAIP3. The majority of mutations represent nonsense mutations and deletions or insertions leading to frameshifts (Fig. 1 D). No mutation hotspot was identified (Tables I and andII;II; and Fig. 1 D). However, most missense mutations are located in the last exon of TNFAIP3 coding for most C-terminal A20 zinc fingers. The question of the oncogenic potential of these amino acid replacements remains open.

Loss of A20 contributes to constitutive NF-κB activity in cHL cell lines

Based on this genetic evidence, TNFAIP3 appears to be a tumor suppressor gene in cHL and PMBL. To functionally test this hypothesis, we expressed A20 by lentiviral gene transfer in cHL cell lines harboring inactivating TNFAIP3 mutations (L-1236 and KM-H2) and as a control in a cHL cell line expressing wild-type A20 (L-428; Fig. 1 A). To this end, we generated lentiviral expression constructs allowing coexpression of GFP and either A20, IκBαS32,36A superrepressor or luciferase by inserting the Thosea asigna insect virus T2A sequence between the coding sequences of the genes. Ribosome skipping occurs during translation of the T2A sequence, leading to generation of two separate proteins from one transcript (23). In the IκBαS32,36A superrepressor, which served in our experiments as a control for NF-κB repression, two serine residues of positions 32 and 36 are replaced by alanines, thus preventing the phosphorylation required for proteasomal degradation of this NF-κB inhibitor (24). To assess A20 expression levels mediated by lentiviruses and efficient ribosome skipping, we analyzed A20 and GFP protein expression of isolated GFP-expressing L-1236 cells by Western blotting. This revealed the efficiency of ribosome skipping mediated by the T2A sequence by the absence of detectable fusion protein (Fig. S3). The analysis also indicated that lentivirally transduced cells express an approximately fourfold level of A20 compared with L-428 cells, expressing endogenous wild-type A20. To investigate the consequences of reconstitution of A20 in cHL cell lines, we FACS sorted 2,500 cells transduced with the respective lentiviruses according to GFP expression, and determined by quantitative PCR (qPCR) mRNA levels of selected NF-κB target genes (BIRC3, ICAM1, and LTA), which are negatively regulated upon inhibition of NF-κB in cHL (2). qPCR values of NF-κB target genes were normalized to GAPDH, and the resulting data from luciferase-expressing cells, which served as negative control, were subtracted from A20- and IκBαS32,36A-transduced cells (ΔΔCt method). Upon reconstitution of A20 in cell lines carrying inactivated TNFAIP3 (KM-H2 and L-1236), we observed a significant decrease of mRNA levels of the selected NF-κB target genes, indicating a globally attenuated transcriptional activity of NF-κB (Fig. 3 A). Mean transcriptional reduction of these genes by A20 ranged between 4.1- and 5.5-fold in KM-H2 and 2.5- and 6.5-fold in L-1236 as compared with cells transduced with luciferase-expressing viruses, respectively. A similar decrease of transcription of NF-κB target genes was seen in KM-H2 and L-1236 cells expressing IκBαS32,36A (mean reduction values = 6.9–9.1 for KM-H2 and 3.1–6.4 for L-1236). Remarkably, besides inactive TNFAIP3, KM-H2 cells also harbor inactivating mutations in NFKBIA (IκBα) (25). In contrast, L-428 cells, strongly expressing endogenous wild-type A20, did not show down-regulation of NF-κB transcriptional activity by A20 gene transfer (mean = 1–1.1), indicating that this increased A20 expression does not impair NF-κB activity in these cells. Notably, L-428 cells carry inactivating mutations in the NF-κB inhibitors NFKBIA and NFKBIE (IκBϵ) (25, 26). In accordance with the known effect of A20 on RIP protein stability, we observed strongly reduced levels of RIP protein in A20-reconstituted L-1236 cells as compared with nonreconstituted L-1236 cells (Fig. S4).

Figure 3.
A20 reconstitution in cHL cell lines harboring inactivating TNFAIP3 mutations results in reduction of transcriptional NF-κB activity and reduction of cellular metabolism. cHL cell lines harboring inactive TNFAIP3 (L-1236 and KM-H2) or wild-type ...

A20 reconstitution confers cytotoxicity to A20-deficient cHL cell lines

To test the consequence of A20 reconstitution on proliferation and/or survival in A20-negative cell lines, we performed an MTS assay with sorted GFP+ cells expressing either A20, IκBαS32,36A, or luciferase. This revealed a strong cytotoxic effect of A20 reexpression in L-1236 cells and a more moderate one in KM-H2 cells compared with the luciferase-expressing negative control cells (Fig. 3 B). In contrast, overexpression of A20 in L-428 cells had little or no effect on these cells (also indicating that the experimental overexpression of A20 per se is not cytotoxic), whereas IκBαS32,36A expression showed strong cytotoxicity (Fig. 3 B and Fig. S5).

Concluding remarks

We identify TNFAIP3 as a novel tumor suppressor gene in cHL and PMBL by showing frequent somatic and clonal biallelic inactivation of the gene, and presenting evidence that loss of A20 function contributes to the constitutive activity of the transcription factor NF-κB and the survival and/or proliferation of the cells. The detection of destructive somatic mutations within the gene and frequent complete inactivation, together with the functional data, are indeed critical findings to verify this hypothesis. The chromosomal region containing TNFAIP3 is recurrently affected by monoallelic deletions in different lymphomas and the fact that, in follicular lymphoma for example, which is not characterized by strong NF-κB activity, monoallelic deletions including TNFAIP3 were found, but no somatic mutations within the gene in 50 cases analyzed (14), indicated that another gene is the target of the deletions in follicular lymphoma.

The striking clustering of unequivocally destructive mutations with EBV cases of cHL defines the first example of a genetic lesion that distinguishes EBV+ from EBV cHL cases. This clustering suggests complementing functions of A20 inactivation and EBV transformation, and thereby supports important pathogenetic roles of both events. On the other hand, there is also indication from the cHL cell lines that multiple transforming events in the NF-κB pathway can cooperate, such as concurrent TNFAIP3 and NFKBIA mutations in KM-H2. This represents one of the few examples in which multiple genetic lesions occur in the same pathway in one tumor clone. Whether concurrent mutations in TNFAIP3 and NFKBIA, which is mutated in ~10–20% of cHL cases (25, 27, 28), also occur in primary cases of cHL remains to be identified. In PMBL, NFKBIA mutations have not been found (29). The important role of A20 as a key regulator of NF-κB activity in multiple immune functions was recently impressively demonstrated (8, 30, 31), and its role as a tumor suppressor gene may well go beyond cHL and PMBL, as shown by the recent detection of TNFAIP3 mutations in marginal zone B cell lymphomas (32).

MATERIALS AND METHODS

Patient samples and cell lines.

Lymph node samples from 54 patients with cHL were collected from the Department of Pathology, Hematopathology Section and Lymph Node Registry at the University of Kiel and the Senkenberg Institute of Pathology at the University of Frankfurt. According to morphological and immunohistochemical criteria, 26 cases were classified as nodular sclerosis cHL, 20 cases were classified as mixed cellularity, 1 case was classified as lymphocyte depleted, 1 case was classified as lymphocyte rich, and 6 cases were unclassified. In cases for sequence analysis, EBV status was determined by LMP1 immunohistochemical staining and/or EBV-encoded RNA in situ hybridization. PMBLs were obtained from the Institute of Pathology at the University of Heidelberg and the Department of Pathology at the University of Kiel. PMBL cell line Karpas-1106P and cHL cell lines L-428, L-591, L-1236, HDLM-2, KM-H2, and the recently established line U-HO1 (33) were analyzed. Approval by the Institutional Review Boards in Kiel and Frankfurt was obtained for these studies.

Laser microdissection and pressure catapulting of HRS and nontumor cells.

5-µm frozen lymph node sections of primary cHL biopsies were mounted on membrane-covered slides (PALM) and stained with anti-CD30 antibody (BerH2; Dako). Single CD30+ HRS cells were microdissected using laser microdissection and pressure catapulting (PALM) into PCR buffer, and pooled into groups of 10–20 cells. Nonneoplastic cells were microdissected in groups of 20–50 cells.

PCR amplification and sequencing of TNFAIP3.

All coding exons of TNFAIP3 were amplified using primer sequences displayed in Table S2. DNA of cell lines and of whole-tissue sections of PMBLs was amplified in one round of PCR using first-round primers and 35 cycles. Nested amplification of the individual exons was performed using identical conditions with internal primers and 40 cycles in the second round of PCR. Sequencing was performed on an ABI 3130 sequencing apparatus (Applied Biosystems).

Immunoblotting.

Western blot analysis was conducted using antibody clone 59A426 (NatuTec) for A20, antibody clone I-19 (Santa Cruz Biotechnology, Inc.) for actin, antibody AB513 for TurboGFP (Evrogen), and antibody clone 38/RIP for RIP (BD), applying standard techniques.

Interphase cytogenetics.

FISH and FICTION analyses were performed as previously described (34) using differently labeled bacterial artificial chromosome clones RP11-783B20 (spanning TNFAIP3 and extending in the centromeric direction, labeled with SpectrumGreen) and (in some cases) RP11-703G8 (spanning TNFAIP3 and extending in the telomeric direction, labeled with SpectrumOrange), as well as a probe for centromere 6 (CEP6, labeled with SpectrumAqua; all from Abbott/Vysis) as an internal control. For FICTION, immunofluorescence with monoclonal anti-CD30 antibody (Dako) detected with an Alexa Fluor 594–conjugated secondary antibody (Invitrogen) was applied in combination with the TNFAIP3-CEP6 probe. Slides were analyzed using a fluorescence microscope (Axio Imager.A1; Carl Zeiss, Inc.) equipped with the appropriate filter sets (AHF) and were documented using an Isis imaging system (MetaSystems). Nuclei from HRS cells were identified by virtue of their larger size, frequent hyperploid genomic status, and CD30 expression (the latter only by FICTION). Cases showing lower copy numbers for TNFAIP3 than for CEP6 were classified as deleted.

SNP microarray analysis.

The genome-wide human SNP Array 6.0 (Affymetrix) was used according to the protocol provided by the manufacturer. Microarrays were washed and stained with the Fluidics Station 450 (Affymetrix) and scanned with the GeneChip Scanner 3000 (Affymetrix) using the Genotyping Console software (version 3.0; Affymetrix). The Birdseed v2 algorithm was used to genotype tumor samples. Copy number analysis, LOH analysis, and segmentation were calculated using Genotyping Console software. Segments with aberrant copy number were considered as copy number aberration only if they consisted of at least 20 consecutive SNPs and comprised a minimal size of 100 kb. Custom 100K GeneChip Mapping SNP Array analyses were performed at Affymetrix. The complete GeneChip datasets have been deposited in the Gene Expression Omnibus under accession no. GSE15264.

Construction of lentiviral vectors.

Lentiviral vectors were constructed by PCR and cloning based on pGIPZshRNAmir (Open Biosystems). A T2A sequence for ribosome skipping was cloned in frame between coding sequences of TNFAIP3, IκBαS32,36A (provided by J. Feuillard, Centre National de la Recherche Scientifique, CHU Dupuytren, Limoges, France) or luciferase (provided by B. Jungnickel, Helmholtz Center Munich, Munich, Germany) and GFP. Expression was driven by a CMV promoter. Virus production was performed by cotransfection of 293T cells using GeneJuice (EMD) with the plasmids pGIPZ-NheI (Fig. S3), psPAX2 (plasmid 12260; Addgene), and pMD2.G (plasmid 12259; Addgene; the latter two were constructed by D. Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland). 3 d after transfection, supernatants were collected and concentrated by ultracentrifugation for 1.5 h at 100,000 g (Beckman Coulter). Titration of lentiviruses was performed using 293T cells.

Transduction of cHL cell lines, FACS sorting, and qPCR of NF-κB target genes.

cHL cell line cells were infected using a multiplicity of infection of 10. GFP+ cells were FACS sorted 80 h after infection, excluding propidium iodide–positive cells. For qPCR analysis, 2,500 GFP-expressing cells were sorted into RLT lysis buffer (QIAGEN). RNA was extracted using the RNeasy Micro Kit (QIAGEN) according to the manufacturer’s instructions. qPCR analysis was conducted using gene expression assays (TaqMan; Applied Biosystems) and analyzed on an ABI Prism 7900HT Fast Real-Time PCR System (Applied Biosystems). For each cell line, transfections and sorting of cells were done at least in triplicates, of which mostly three or four independent qPCR measurements were performed.

MTS assay.

cHL cell line cells were FACS sorted 80 h after infection in aliquots of 10,000 GFP-expressing cells in PBS/0.5% BSA. After centrifugation, the aliquots were resuspended in 100 µl of conditioned culture medium each in a 96-well plate. After 48 h, CellTiter 96 AQueous One Solution reagent (Promega) was added to each well and analyzed at 490 nm. The conversion of MTS into a formazan product was accomplished by dehydrogenase enzymes found in metabolically active cells. The quantity of formazan product as measured by the amount of 490-nm absorbance is directly proportional to the number of living cells in culture.

Online supplemental material.

Genome-wide human SNP Array 6.0 analyses of cHL cell lines revealing deletions and LOH at the TNFAIP3 locus are shown in Fig. S1. Fig. S2 exemplifies TNFAIP3 sequences of primary HRS cells, PMBLs, and corresponding nontumor cells. Expression and function of the A20-T2A-GFP lentiviral construct is shown in Fig. S3. Fig. S4 shows reduction of RIP protein levels upon A20 reexpression in L-1236 cells. Additional data on A20 reconstitution in cHL cell lines harboring inactivating TNFAIP3 mutations are depicted in Fig. S5. Table S1 provides detailed results of the interphase cytogenetic analyses. Table S2 shows primer sequences used for seminested two-round genomic DNA amplification of TNAIP3. Online supplemental material is available at http://www.jem.org/cgi/content/full/jem.20090528/DC1.

Acknowledgments

We thank E. Tiacci, E. Maggio, B. Jungnickel, I. Pfeil, M. Grez, and I. Vogler for helpful discussions; J. Feuillard, B. Jungnickel, and D. Trono for reagent supply; and G. Lorenz, K. Lennartz, K. Waldhelm, R. Zühlke-Jenisch, and R. Lieberz for excellent technical assistance. We thank F. Chen for inspiring this work with his excellent review.

This work was supported by grants from the Wilhelm Sander-Stiftung (2005.168.2), the Deutsche Krebshilfe, the Mildred Scheel-Stiftung (107736 and 107748), the Deutsche Forschungsgemeinschaft, and the Kinder-Krebs-Initiative Buchholz/Holm Seppensen.

The authors have no conflicting financial interests.

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