• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Oct 1984; 20(4): 684–686.
PMCID: PMC271411

Enzymatic profiles of Enterobacter sakazakii and related species with special reference to the alpha-glucosidase reaction and reproducibility of the test system.

Abstract

The enzymatic profiles of Enterobacter sakazakii, Enterobacter cloacae, Enterobacter aerogenes, and Enterobacter agglomerans were determined with the API ZYM system (API System S.A., La Balme Les Grottes, France). Each assay was performed three times. A simple formula was derived and applied to assess the reproducibility of the API ZYM tests. In addition, a separate alpha-glucosidase test was performed. All E. sakazakii isolates produced alpha-glucosidase, in contrast to the other Enterobacter isolates. No phosphoamidase activity was detected in any of the E. sakazakii isolates, whereas it was present in 72% of E. cloacae, 89% of E. agglomerans, and 100% of E. aerogenes isolates. It was concluded that detection of alpha-glucosidase permits rapid and reliable differentiation between E. sakazakii and other Enterobacter species. The reproducibilities of alpha-glucosidase and phosphoamidase reactions were estimated to be 89 and 81%, respectively.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (626K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aldová E, Hausner O, Postupa R. Tween-esterase activity in Enterobacter sakazakii. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Nov;256(1):103–108. [PubMed]
  • Abt C, Dietrich M, Niethammer D, Arnold R, Pflieger H, Töllner U, Vanek E, Kubanek B, Kleihauer E, Heimpel H. Akute arzneimittelinduzierte Agranulozytose. Vergleich zwischen herkömmlicher Krankenhausbehandlung und antimikrobieller Dekontamination im Plastikisolierbettsystem. Eine retrospektive Studie über 30 beobachtete Fälle. Dtsch Med Wochenschr. 1978 Jan 20;103(3):108–114. [PubMed]
  • Humble MW, King A, Phillips I. API ZYM: a simple rapid system for the detection of bacterial enzymes. J Clin Pathol. 1977 Mar;30(3):275–277. [PMC free article] [PubMed]
  • Joker RN, Norholm T, Siboni KE. A case of neonatal meningitis caused by a yellow enterobacter. Dan Med Bull. 1965 Sep;12(5):128–130. [PubMed]
  • Kleiman MB, Allen SD, Neal P, Reynolds J. Meningoencephalitis and compartmentalization of the cerebral ventricles caused by Enterobacter sakazakii. J Clin Microbiol. 1981 Sep;14(3):352–354. [PMC free article] [PubMed]
  • Monroe PW, Tift WL. Bacteremia associated with Enterobacter sakazakii (yellow, pigmented Enterobacter cloacae). J Clin Microbiol. 1979 Dec;10(6):850–851. [PMC free article] [PubMed]
  • Muytjens HL, Kollée LA. Neonatal meningitis due to Enterobacter sakazakii. Tijdschr Kindergeneeskd. 1982 Aug;50(4):110–112. [PubMed]
  • Muytjens HL, Zanen HC, Sonderkamp HJ, Kollée LA, Wachsmuth IK, Farmer JJ., 3rd Analysis of eight cases of neonatal meningitis and sepsis due to Enterobacter sakazakii. J Clin Microbiol. 1983 Jul;18(1):115–120. [PMC free article] [PubMed]
  • URMENYI AM, FRANKLIN AW. Neonatal death from pigmented coliform infection. Lancet. 1961 Feb 11;1(7172):313–315. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...