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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Immunity. Author manuscript; available in PMC Jul 21, 2009.
Published in final edited form as:
PMCID: PMC2713656

MicroRNA-155 Suppresses Activation-Induced Cytidine Deaminase-Mediated Myc-Igh Translocation


MicroRNAs (miRNAs) are small noncoding RNAs that regulate vast networks of genes that share miRNA target sequences. To examine the physiologic effects of an individual miRNA-mRNA interaction in vivo, we generated mice that carry a mutation in the putative microRNA-155 (miR-155) binding site in the 3′-untranslated region of activation-induced cytidine deaminase (AID), designated Aicda155 mice. AID is required for immunoglobulin gene diversification in B lymphocytes, but it also promotes chromosomal translocations. Aicda155 caused an increase in steady-state Aicda mRNA and protein amounts by increasing the half-life of the mRNA, resulting in a high degree of Myc-Igh translocations. A similar but more pronounced translocation phenotype was also found in miR-155-deficient mice. Our experiments indicate that miR-155 can act as a tumor suppressor by reducing potentially oncogenic translocations generated by AID.


Antibody genes are assembled in developing B cells by RAG1-RAG2 recombinase-mediated site-specific recombination of immunoglobulin (Ig) variable (V), diversity (D), and joining (J) gene segments. Although V(D)J recombination produces a diverse repertoire of IgM antibodies, high-affinity IgG antibody responses require further Ig diversification by somatic hypermutation (SHM) and class-switch recombination (CSR) in antigen-activated B cells (Klein and Dalla-Favera, 2008; Meffre et al., 2000; Rajewsky, 1996). These reactions are both initiated by activation-induced cytidine deaminase (AID), which deaminates cytosine residues and introduces U:G mismatches in DNA (Di Noia and Neuberger, 2007; Muramatsu et al., 2007). AID activity is primarily restricted to Ig genes but can also produce off-target lesions in non-Ig sites such as oncogenes (Liu et al., 2008), and the double-strand breaks it creates can be substrates for translocation (Dorsett et al., 2007; Ramiro et al., 2004). Therefore, the regulation of this enzyme is essential to maintain genomic integrity.

MicroRNAs (miRNAs) are a class of ~20–23 nt noncoding RNAs produced from genes encoding RNAs with a hairpin secondary structure (Bartel, 2004; Filipowicz et al., 2008; Meister and Tuschl, 2004). The hairpin RNAs are processed to produce mature single-stranded small RNAs (the microRNA) that are then incorporated into protein complexes termed, miRNPs, that contain one of several different Argonaute proteins (Filipowicz et al., 2008; Meister and Tuschl, 2004). miRNA binding to complementary sequences, usually located in the 3′-untranslated regions (3′ UTRs) of messenger RNAs (mRNAs), can regulate expression of large groups of genes by a variety of mechanisms including cleavage, degradation, cell-cycle control, translational inhibition, and mRNA transport (Bartel, 2004; Filipowicz et al., 2008; Leung and Sharp, 2007; Meister and Tuschl, 2004).

miR-155 is an oncogenic miRNA product of the MIRN155 gene in humans or Bic in mice and is deregulated in a number of different cancers, most of which are of B cell origin (Costinean et al., 2006; Eis et al., 2005; Fulci et al., 2007; Gironella et al., 2007; Iorio et al., 2005; Jay et al., 2007; Kluiver et al., 2005, 2006, 2007; Lagos-Quintana et al., 2002; Landgraf et al., 2007; Lawrie et al., 2007; Lee et al., 2007; Marton et al., 2008; Nikiforova et al., 2008; Roldo et al., 2006; Tam, 2001; Volinia et al., 2006). Overexpression of miR-155 in transgenic mice leads to preleukemic pre-B cell proliferation in bone marrow and spleen, followed by high-grade B cell neoplasms (Costinean et al., 2006). Conversely, the mature form of miR-155 is absent in primary cases of Burkitt’s lymphoma (Kluiver et al., 2006, 2007; Landgraf et al., 2007). Although the precise role of miR-155 in promoting lymphomagenesis has not been determined, this miRNA is an important immune modulator whose expression is induced along with AID in activated leukocytes and germinal center B cells (Haasch et al., 2002; Taganov et al., 2006; Tam, 2001; Thai et al., 2007; van den Berg et al., 2003). miR-155 deficiency in mice leads to the deregulated expression of hundreds of mRNAs, some of which are direct targets of miR-155, resulting in abnormalities in the germinal center reaction and antibody responses in vivo (Rodriguez et al., 2007; Thai et al., 2007; Vigorito et al., 2007).

Aicda encodes AID and contains a single miR-155 binding site in its 3′ UTR (John et al., 2004; Lewis et al., 2005). It is among the genes deregulated in miR-155-deficient B cells, where its expression is increased 1.6-fold as measured by gene array (Vigorito et al., 2007). However, this small change in mRNA expression was not confirmed by a direct measurement, and whether this is a direct or indirect effect of miR-155 deletion has not been determined. With a few exceptions, miRNA control in living organisms has been studied by overexpression and deletion experiments (Choi et al., 2007; Mayr et al., 2007). These approaches result in simultaneous effects on many genes that share miRNA target sequences, complicating interpretation because indirect regulators are themselves miRNA targets.

To determine whether miR-155 regulates AID expression directly, and to investigate the potential significance of that regulation in Burkitt’s lymphoma-associated Myc-Igh translocation, we produced a mouse strain that carries a mutation in the putative miR-155 target site in the 3′ UTR of the gene encoding AID (Aicda155 mice) and compared them to miR-155-deficient mice.


Aicda155 Mice

Like Aicda, miR-155 appears to have emerged in evolution in bony fish and the 3′ UTR of Aicda contains a candidate miR-155 binding site that is conserved between fish and humans, suggesting that the two may have coevolved (Figures S1A–S1C available online) (Barreto et al., 2005; Conticello et al., 2005, 2007; Lewis et al., 2003, 2005; Wakae et al., 2006). To examine the effects of miR-155 on AID expression directly, we replaced the conserved miR-155 target sequence (seed-match nucleotides 1–8) in the 3′ UTR of Aicda with a G- and C-rich nucleotide sequence that does not match the seed sequence of any known miRNAs (Figures S1A and S2). The mutation was confirmed by sequencing of the Aicda mRNA from the mutant mice (not shown). Aicda155/+ mice were bred to C57Bl/6 Aicda−/− mice to produce Aicda155/− mice that were born at normal frequencies (not shown). When compared to wild-type or Aicda+/− controls by flow cytometry, Aicda155/− showed normal B cell development in the bone marrow and normal numbers of peripheral B cells in spleen (Figure S3A). In addition, the amount of serum-immunoglobulin isotypes was normal as measured by enzyme-linked immunoassays (Figure S3B).

Aicda155 and miR-155-Deficient Mice Express Higher Amounts of AID Protein

To determine whether the Aicda155 mutation altered AID protein expression, we stimulated B cells with lipopolysacharide (LPS) and interleukin 4 (IL-4) in vitro to induce AID and miR-155, and we measured AID protein expression over 4 days (Figures 1A and 1B). In control B cells, AID protein expression was initially detected 2 days after stimulation and increased on days 3 and 4 in culture (Figures 1A and 1B, and data not shown). Aicda155/− showed a similar expression pattern, but in all cases the amounts were 2- to 3-fold higher than in Aicda+/− controls, as determined by immunoblot (Figures 1A and 1B). Similar effects were also found in miR-155-deficient B cells (Figures 1C and 1D). We conclude that miR-155 regulates the amount of AID protein in stimulated B cells.

Figure 1
Aicda155 Mice

miR-155 Destabilizes Aicda mRNA

Consistent with elevated amounts of AID protein, the corresponding mRNA was elevated in Aicda155/− when compared to Aicda+/− controls beginning 2 days after stimulation with LPS and IL-4 (Figure 1E). Similar results were found with miR-155-deficient B cells, which show a 2.5-fold increase in Aicda mRNA after 4 days (Figure 1F). Increased Aicda mRNA expression suggests that miR-155 regulates the expression of this gene by altering messenger stability. To determine whether miR-155 regulates Aicda mRNA stability, we stimulated B cells with LPS and IL-4, blocked transcription with Actinomycin-D, and measured the decay of Aicda mRNA (Figures 1G and 1H). We found that the half-life of Aicda transcripts was increased from 1.05 hr in control to 1.94 hr in the mutant as determined by linear regression analysis of two mice assayed in triplicate, indicating that miR-155 regulates the level of Aicda mRNA by increasing its turnover (Figures 1G and 1H).

Class Switching in Aicda155 and miR-155-Deficient Mice

To examine the effects of Aicda155 on class-switch recombination, we labeled B cells with 5-(6)-carboxyfluorescein diacetate succinimidyl diester (CFSE), a reporter dye for cell division, and stimulated them with LPS and IL-4. Cell-surface IgG1 expression was monitored by flow cytometry over a time course of 4 days in culture. Although cell division was normal, class switching was enhanced in Aicda155/− when compared to Aicda+/− B cells and was similar to Aicda+/+ controls (Figure 2A). A similar increase in switching was seen in Aicda155/155 when compared to Aicda+/+ (Figure 2B). This effect was most pronounced early in the culture period, when the number of IgG1-expressing Aicda+/+ and Aicda155/− cells was nearly double that of Aicda+/− controls (Figure 2A). In contrast, miR-155-deficient B cells showed a subnormal amount of class switching despite increased AID expression (Figures 1C, 1D, and and2C)2C) (Rodriguez et al., 2007; Thai et al., 2007; Vigorito et al., 2007)). We conclude that a 2- to 3-fold increase in AID protein expression leads to increased class switching in vitro in Aicda155/− but not miR-155-deficient B cells.

Figure 2
Class-Switch Recombination by Aicda155 and miR-155-Deficient B Cells

Somatic Hypermutation in Aicda155 Mice

Class switching is associated with AID-induced somatic mutations in the 5′ of the switch μ (Sμ) region (Petersen et al., 2001). To determine whether Aicda155 also alters the production of AID-mediated lesions in the Igh switch regions, we measured the mutations that occur 5′ of the switch μ region in LPS- and IL-4-stimulated B cells that had undergone five cell divisions (Petersen et al., 2001). The small increase in mutations in Aicda155/− B cells 5′ of the switch μ region was not statistically significant but corresponded to the increase in class switching at the same time point (Figure 2D).

AID expression is also necessary to induce somatic hypermutation of immunoglobulin genes (Muramatsu et al., 2000; Revy et al., 2000; Yoshikawa et al., 2002). To examine the effects of Aicda155 on somatic mutation in vivo, we immunized mice and purified germinal-center B cells, which actively mutate their Ig genes. Like LPS- and IL-4-activated B cells, Aicda155/− germinal-center B cells contained higher amounts of Aicda mRNA than controls (Figure 3A). Similar to 5′ of the switch μ region, we found a statistically insignificant effect on somatic hypermutation of the noncoding DNA region 3′ of IgJH4, which cannot be selected for or against during the germinal-center reaction (Jolly et al., 1997) (Figures 2D and and3B).3B). Although Bcl6 is also mutated during the germinal-center reaction (Liu et al., 2008), we found no increase in mutation at this locus in Aicda155/− germinal-center B cells (Figure S4). In conclusion, neither the miR-155 deficiency (Thai et al., 2007; Vigorito et al., 2007) nor Aicda155 mutation significantly increases somatic hypermutation despite elevated AID expression, and therefore this process is probably regulated by additional mechanisms.

Figure 3
Aicda mRNA Expression and Somatic Hypermutation in Germinal-Center B Cells from Aicda155 Mice

Aicda155 mRNA and Protein Do Not Persist in Plasmablasts

To determine whether Aicda155 would result in persistence of Aicda mRNA in plasmablasts, where it is not normally transcribed, we cultured B cells under conditions where they undergo class switching and develop into CD138 plasmablasts (Horikawa and Takatsu, 2006) (Figure 2E). As in LPS and IL-4 cultures, Aicda155 enhanced switching to IgG1 but did not alter plasmablast development (Figure 2E). Furthermore, the amount of Aicda mRNA expressed in plasmablasts was two orders of magnitude less than in B cells stimulated with LPS and IL-4 (Figure 2F). Thus, Aicda155 increases AID expression in developing B cells, yet it does not extend AID expression into the plasmablast stage.

Myc-Igh Translocations in Aicda155 and miR-155-Deficient B Cells

In addition to class-switch recombination and somatic mutation, AID induces potentially oncogenic reciprocal chromosome translocation between Igh and Myc (Myc-Igh) (Dorsett et al., 2007; Ramiro et al., 2004, 2006). To examine the effect of Aicda155 on these translocations, we assayed stimulated B cells for aberrant juxtaposition of the chromosomes that carry Myc and Igh (Potter, 2003; Ramiro et al., 2004) (Figure 4A). In contrast to the modest effects on somatic mutation, Aicda155/− B cells showed a 3- to 6-fold increase in translocation frequency compared to Aicda+/− controls (Figures 4B and 4C, and data not shown). A similar increase in translocation was seen in Aicda155/155 compared to wild-type controls (Figure 4D). Therefore, the mechanisms that restrict somatic mutation in cells expressing elevated amounts of AID do not limit translocation.

Figure 4
Igh to Myc Translocations in Stimulated B Cells from Aicda155 Mice

In addition to Aicda, miR-155 is predicted to target numerous different genes that play a role in the maintenance of genomic integrity (John et al., 2004; Lewis et al., 2005). To determine whether deregulation of miR-155 might further increase the frequency of AID-dependent Myc-Igh translocations, we assayed miR-155-deficient B cells. We found a ~15-fold enhanced translocation frequency in the absence of miR-155 compared to matched controls (Figures 4F and 4G). Thus, miR-155 expression in activated B cells suppresses Myc-Igh translocations.


AID initiates Ig gene diversification in activated B cells by deaminating cytosine to produce U:G mismatches in DNA (Di Noia and Neuberger, 2007; Muramatsu et al., 2007). These lesions are processed by ubiquitous DNA-repair pathways and error-prone polymerases to produce somatic mutations or double-strand DNA breaks, which are obligate intermediates in immunoglobulin class-switch recombination (Di Noia and Neuberger, 2007; Muramatsu et al., 2007). However, in addition to diversifying immune responses, AID can damage the genome by mutating oncogenes and creating substrates for chromosomal translocations (Dorsett et al., 2007; Klein and Dalla-Favera, 2008; Perez-Duran et al., 2007; Ramiro et al., 2004). Indeed, AID is essential for translocation between Myc and Igh, and the frequency of these rare off-target events is directly related to the level of AID expressed (Dorsett et al., 2007; Ramiro et al., 2006; Ramiro et al., 2004). For example, Myc-Igh translocations are rare events in B cells expressing physiological levels of AID (1 in 3 × 107 cells) and increase in frequency to (1 in 2.5 × 104) in B cells overexpressing AID (Ramiro et al., 2006). It is therefore essential that AID expression is tightly regulated in vivo.

Under physiologic circumstances, AID expression is restricted to activated B cells (Gonda et al., 2003; Sayegh et al., 2003), and its concentration in the nucleus is limited by active export (McBride et al., 2004). Furthermore, posttranslational modification by phosphorylation at position S38 is required for interaction between AID and replication protein A for optimal AID activity on transcribed DNA (Basu et al., 2005; Chatterji et al., 2007; McBride et al., 2006; Pasqualucci et al., 2006). The results presented here and in an accompanying paper in this issue of Immunity (Teng et al., 2008) define an additional mechanism for regulating AID expression in vivo. Aicda mRNA half-life is decreased by a single miR-155 binding site within the 3′ UTR. Our results also highlight phenotypic differences between the miRNA binding-site mutation in Aicda and the deletion of miR-155. Both mutations result in 2- to 3-fold-higher levels of Aicda mRNA and protein in activated B cells. In contrast, Aicda155 is associated with increased class-switch recombination, whereas mice with a deficiency in miR-155 show an unexpected decrease in this reaction (Thai et al., 2007). Nevertheless, both mutations result in a substantial increase in Myc-Igh translocations. In the case of miR-155-deficient B cells, translocation frequency is similar to that found in ataxia-telangiectasia-mutated kinase (ATM)-deficient B cells, which also display reduced amounts of class-switch recombination (Ramiro et al., 2006; Reina-San-Martin et al., 2004). The disproportionate increase in Myc-Igh translocations underlines the importance of AID regulation and suggests that these events are not limited by the same factors that restrict the degree of SHM and CSR.

Overexpression of Myc in B cells in transgenic mice results in B cell lymphomas (Adams et al., 1985; Schmidt et al., 1988). However, transgenic overexpression of AID does not (Muto et al., 2006), and it is therefore not surprising that we have not found lymphomas in Aicda155 or miR-155-deficient mice after 3 months and 1 yr of observation, respectively.

miRNAs regulate expression of large groups of genes by a variety of mechanisms including cleavage, stability, cell-cycle control, translation inhibition, and mRNA transport (Bartel, 2004; Filipowicz et al., 2008; Leung and Sharp, 2007; Meister and Tuschl, 2004). It is therefore not surprising that they have been implicated in malignant transformation (Esquela-Kerscher and Slack, 2006; Hammond, 2007; Thomson et al., 2006). By mutating the 3′ UTR in Aicda, we have isolated the effects of miR-155 on a single mRNA and determined that it destabilizes the message, which in turn reduces the amount of AID protein and the frequency of Myc-Igh translocations. Throughout evolution, miR-155 and its binding site in the 3′ UTR of Aicda have been conserved. Therefore, we speculate that the emergence of miR-155 and AID in vertebrates and the conservation of their interaction through evolution are related to minimizing chromosome translocations.

miR-155 mutant mice showed higher levels of translocation than Aicda155, indicating that miR-155 may target additional mRNAs that cooperate to prevent Myc-Igh translocation. These translocations are the key transforming events in Burkitt’s lymphoma (Klein and Dalla-Favera, 2008) and are known to be deficient in miR-155 as a result of defects in processing of the gene encoding miR-155 (Kluiver et al., 2006; Kluiver et al., 2007; Landgraf et al., 2007). Thus, our findings suggest a molecular rationale for the development of human Burkitt’s lymphoma, in that absence of miR-155 predisposes activated B cells to Myc-Igh translocations by increasing expression of AID and yet-to-be-identified proteins that contribute to maintaining genomic integrity (Di Noia and Neuberger, 2007; Kluiver et al., 2007; Kuppers and Dalla-Favera, 2001).



Aicda nucleotides AGCATTAA, located in the Aicda 3′ UTR, 468 bp downstream of the stop codon, were replaced with GCGCGCGC by gene targeting (Figure S1A). The long arm of the targeting vector was 6.9 kb long and introduced a LoxP site within the intron between Aicda exons 4 and 5 (Figure S2). The short arm was a 1.5 kb fragment extending downstream of the 3′ UTR. A LoxP-flanked neomycin-resistance gene was used for positive selection, and a diphtheria toxin gene was used for negative selection (Yagi et al., 1990). The targeting construct was linearized and transfected into C57Bl/6 embryonic stem cells (ESCs). ESC clones were screened and seven positive clones were injected into C57Bl/6 blastocysts, and one produced chimeric mice that transmitted the mutation. The genotype was confirmed by amplifying the mutation with a primer external to the targeting construct and proximal to the end of the short arm. The resulting amplification product was verified by sequencing and digesting with AsceI, resulting in a digested wild-type allele amplification product and nondigested Aicda155 product. The identity of the resulting Aicda transcript was confirmed by reverse transcription of total RNA from activated B cells followed by amplification of the Aicda transcript from the 5′ UTR to the 3′ UTR at the end of the short arm and then by sequencing. To produce Aicda155/− mice, we crossed heterozygous Aicda155/+ mice to Aicda−/− C57Bl/6 mice and used littermate Aicda+/− as controls. miR-155-deficient mice were previously described (Thai et al., 2007). All mice were maintained under specific pathogen-free conditions, and experiments were performed under Rockefeller University IACUC approved protocols.

Lymphocyte Cultures and Translocation Assays

Resting B lymphocytes were isolated from the spleen with CD43 microbeads (Miltenyi Biotech), cultured in RPMI supplemented with L-glutamine, sodium pyruvate, 50 μM 2-Mercaptoethanol, and 10% FBS (GIBCO-BRL), and where indicated cells were labeled with CFDA-SE (5 μM, Molecular Probes). The B cells were stimulated with LPS (25 μg/ml) and IL-4 (5 ng/ml, Sigma) alone or together with IL-5 (15 ng/ml, PharMingen) and BAFF (10 ng/ml, R&D systems) for production of plasmablasts (Horikawa and Takatsu, 2006). For the plasma cell experiment, CD138+ B cells were sorted, and FACS was done on day 5. Transloaction assays were exactly as previously described (Dorsett et al., 2007; Ramiro et al., 2006). In brief, PCR of wild-type, Aicda−/−, miR-155 deficient, Aicda155/−, or Aicda155/155 was performed with total DNA prepared from day 3 or 4 LPS + IL4 cultures. Data shown for Aicda155/− and Aicda155/155 were from day 4 cultures without dead cell removal prior to DNA preparation. Data for miR-155-deficient mice were from day 3 cultures with dead cell removal prior to DNA preparation. Approximate cell number for each sample was determined by DNA quantitation on an ethidium-bromide-stained agarose gel (500 ng DNA = ~100,000 cells). Subsequent amplification of Myc to Igh switch-region translocations was done as previously described. Amplification products were verified with Southern blots by probing for Myc and Igh as previously described (Ramiro et al., 2004). Bands that probe for both Myc and Igh represent Myc to Igh chromosomal translocations.

Flow Cytometry

Single-cell suspensions from bone marrow, spleen, or lymph node were incubated with biotin-conjugated monoclonal antibodies anti-CD43, anti-IgM, anti-B220, anti-CD95, anti-GL7, anti-CD19, anti-FAS, or anti-IgG1 and then stained with streptavidin conjugated to FITC, PE, or APC (BD Biosciences). Germinal-center cells were CD19+, Fas+, and GL-7+ cells sorted from lymph nodes 14 days after immunization. Data were collected with a FACSCalibur and analyzed with CellQuest and FloJo software. Cell sorting was on a FACSAria and FACSVantage.

Immunizations and ELISA

Age- and sex-matched 8- to 12-week-old mice were immunized by footpad injection with 50 μg of alum precipitated NP21-CGG (both from Biosearch Technologies). To measure serum-antibody amounts, we used goat anti-mouse Ig (H+L) for capture and horseradish peroxidase (HRP)-conjugated goat anti-mouse isotype-specific antibodies (Southern Biotechnology) for detection. Values were calculated by comparison with mouse immunoglobulin standards (Southern Biotechnology). Serial dilutions were performed for each sample, and readings were taken within the linear range for each sample and adjusted for dilution. Results reflect relative absorbance for each sample compared with the standard control. All plates were developed with a Peroxidase Substrate Kit (Bio-Rad), and absorbance was measured at 415 nm.


AID antibody was an affinity-purified polyclonal antibody against the carboxyl terminus (EVDDLRDAFRMLGF) of AID and was previously described (McBride et al., 2004). For immunoblot assays, cells were lysed in 20 mM Tris (pH 8.0), 200 mM NaCl, 1% NP-40, 0.5% Deoxycholate, 0.1% SDS, 1 mM DTT, 0.5 mM EDTA, 1 mM PMSF, Protease inhibitor cocktail (Sigma). Fifty micrograms of protein was immunoblotted with the AID antibody or tubulin antibody (abcam). Band densities were quantified with ImageJ software, and relative AID amount is a comparison of AID/tubulin ratios within each gel.

Quantitative PCR

Total RNA was isolated from sorted or LPS- and IL4-activated cells with TRIzol reagent (Life Tecchnologies) according to the manufacturer’s instructions. The first-strand cDNA synthesis was performed with 200 ng of total RNA primed with random primers via the RT reaction protocol provided by the manufacturer (Invitrogen). qPCR was performed with Brilliant SYBR Green QPCR master mix (Stratagene) containing 500 nM primers via standard amplification procedure. All samples were analyzed in triplicate and normalized to GAPDH levels, and the result expressed as n-fold induction compared to wild-type day 4 control. Primers for Aicda were forward 5′-GAAAGTCACGCTGGAGACC G-3′ and reverse 5′-TCTCATGCCGTCGCTTGG-3′, and primers for GAPDH were forward 5′-TGAAGCAGGCATCTGAGGG-3′ and reverse 5′-CGAAGGTGGAAGAGTGGGAG-3′. To determine the half-life of Aicda155/− mRNA, we treated B cells cultured with LPS + IL-4 for 72 hr when Actinomycin D was added to the culture medium at 10 μg/ml final concentration for 0.5, 1, 1.5, 2, 3, or 4 hr. First-strand cDNA synthesis was performed with 200 ng of total RNA primed with random primers (Invitrogen). Triplicate Aicda q-PCR were preformed for each time point for each of two mice and normalized to GAPDH.

Calculation of Translocation p Values and Aicda RNA Half-Life

p value was calculated with a two-tailed Fisher’s exact test. Comparison between genotypes of the number of translocations per number of PCR reactions was used to do the calculations. To determine the Aicda RNA half-life, we used an exponential regression model of the data generated by Excel. That exponential decay models the data correctly is supported by high R2 values (0.93 for Aicda155/− and 0.94 for Aicda+/−) in the regression and the finding that the log plot of the normalized data is linear. The half-life was determined from the slope of the resulting lines.

Mutation Analysis

Genomic DNA from sorted CD19+Fas+GL7+ germinal-center cells of NP-KLH-immunized mice was PCR amplified in 50 μl with PfuTurbo (Stratagene) for 30 cycles from 10,000–100,000 sorted cell equivalents in four independent reactions that were pooled for cloning experiments. For 5′ Sμ, the primers and PCR conditions have been described (Jolly et al., 1997; Reina-San-Martin et al., 2003). The JH4 intron was amplified with 5′-GGAATTCGCCTGACATCTGAGGACTCTGC-3′ and 5′-CTGGACTTTCGGTTTGGTG-3′ for 14 cycles at 94 °C (30 s), 55°C (30 s), and 72°C (90 s) and then with 5′-GGTCAAGGAACCTCAGTCA-3′ and 5′-TCTCTAGACAGCAACTAC-3′ for 21 cycles at 94°C (30 s), 55°C (30 s), and 72°C (30 s). Statistical significance was determined by a two-tailed t test assuming unequal variance. Bcl6 primers were p369 5′-CTTTCTTGGTTGGAGTCGAGG-3′ and p370 5′-CGGGCTTGAGGTCATTTCTC-3′, as previously described in (Muto et al., 2006). PCR reactions were performed in triplicates, the products were pooled, and bands at the expected size were gel extracted and cloned with TOPO-TA (Invitrogen). Bacterial colonies were sequenced by Biotic Solutions and analyzed with CodonCode Aligner software (CodonCode Coporation). Only good-quality sequence was considered, as determined by inspection of the chromatograms.

Supplementary Material



We thank D. Dorsett, M. Dorsett, D. Scheinker for suggestions; K. Velinzon and T. Shengelia for cell sorting; and D. Bosque for animal husbandry. This work was supported by the Schering Foundation (T.A.S.), the Leukemia and Lymphoma Society (D.F.R.), and grants from National Institutes of Health AI064345 (to K.R.) and AI06231 (to M.C.N.). M.C.N is a Howard Hughes Medical Institute investigator.


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