• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Jun 1984; 19(6): 831–833.
PMCID: PMC271193

Unusual susceptibility of methicillin-resistant Staphylococcus aureus to erythromycin, clindamycin, gentamicin, and tetracycline at 30 degrees C but not at 35 degrees C.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen in hospital environments, and optimal detection of MRSA requires nonroutine methods in clinical microbiology laboratories. One such method is an incubation temperature of 30 degrees C in contrast to the more commonly used temperature of 35 degrees C. To determine the percentage of MRSA isolates that would be missed if only one temperature were used, we evaluated methicillin resistance and susceptibility of 2,397 S. aureus isolates by agar dilution at 30 and 35 degrees C. Of the clinical isolates, 93% showed matching MICs of methicillin at both temperatures. Another 6.8% (162) showed different MICs at 30 and 35 degrees C, with 60 of the 162 isolates (2.5% of all isolates studied) being resistant to methicillin at 30 degrees C but susceptible at 35 degrees C (temperature-discrepant MRSA). MICs of other antimicrobial agents, measured at 35 degrees C, revealed an unusual pattern of susceptibility of these temperature-discrepant MRSA isolates. In contrast to previously reported resistance of MRSA at 35 degrees C to erythromycin, clindamycin, gentamicin, and tetracycline, the temperature-discrepant MRSA isolates were susceptible to these agents. This resistance pattern may be of value in identifying questionable MRSA isolates when only one incubation temperature is used.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (494K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aldridge KE, Janney A, Sanders CV, Marier RL. Interlaboratory variation of antibiograms of methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains with conventional and commercial testing systems. J Clin Microbiol. 1983 Nov;18(5):1226–1236. [PMC free article] [PubMed]
  • Annear DI. The effect of temperature on resistance of Staphylococcus aureus to methicillin and some other antibioics. Med J Aust. 1968 Mar 16;1(11):444–446. [PubMed]
  • BARBER M. NATURALLY OCCURING METHICILLIN-RESISTANT STAPHYLOCOCCI. J Gen Microbiol. 1964 May;35:183–190. [PubMed]
  • Barrett FF, McGehee RF, Jr, Finland M. Methicillin-resistant Staphylococcus aureus at Boston City Hospital. Bacteriologic and epidemiologic observations. N Engl J Med. 1968 Aug 29;279(9):441–448. [PubMed]
  • Bulger RJ. A methicillin-resistant strain of Staphylococcus aureus. Clinical and laboratory experience. Ann Intern Med. 1967 Jul;67(1):81–89. [PubMed]
  • Canawati HN, Sapico FL, Montgomerie JZ, Zucchero J. Temperature effect on cephalothin sensitivity of methicillin-resistant Staphylococcus aureus. Am J Clin Pathol. 1981 Mar;75(3):391–394. [PubMed]
  • Canawati HN, Witte JL, Sapico FL. Temperature effect on the susceptibility of methicillin-resistant Staphylococcus aureus to four different cephalosporins. Antimicrob Agents Chemother. 1982 Jan;21(1):173–175. [PMC free article] [PubMed]
  • Cleary TJ, Maurer D. Methicillin-resistant Staphylococcus aureus susceptibility testing by an automated system, Autobac I. Antimicrob Agents Chemother. 1978 May;13(5):837–841. [PMC free article] [PubMed]
  • COLLEY EW, MCNICOL MW, BRACKEN PM. METHICILLIN-RESISTANT STAPHYLOCOCCI IN A GENERAL HOSPITAL. Lancet. 1965 Mar 13;1(7385):595–597. [PubMed]
  • Collins JK, Mader JT, Kelly MT. Resistance of methicillin-resistant staphylococcus aureus to third-generation cephalosporins. J Infect Dis. 1983 Mar;147(3):591–591. [PubMed]
  • Crossley K, Loesch D, Landesman B, Mead K, Chern M, Strate R. An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. I. Clinical studies. J Infect Dis. 1979 Mar;139(3):273–279. [PubMed]
  • Hewitt JH, Coe AW, Parker MT. The detection of methicillin resistance in Staphylococcus aureus. J Med Microbiol. 1969 Nov 4;2(4):443–456. [PubMed]
  • Klimek JJ, Marsik FJ, Bartlett RC, Weir B, Shea P, Quintiliani R. Clinical, epidemiologic and bacteriologic observations of an outbreak of methicillin-resistant Staphylococcus aureus at a large community hospital. Am J Med. 1976 Sep;61(3):340–345. [PubMed]
  • Markowitz N, Pohlod DJ, Saravolatz LD, Quinn EL. In vitro susceptibility patterns of methicillin-resistant and-susceptible Staphylococcus auerues strains in a population of parenteral drug abusers from 1972 to 1981. Antimicrob Agents Chemother. 1983 Mar;23(3):450–457. [PMC free article] [PubMed]
  • McHenry MC, Gavan TL, Farmer RG, Evarts CM. Infection due to methicillin-resistant Staphylococcus aureus. Report of an unusual case. Cleve Clin Q. 1969 Jan;36(1):9–16. [PubMed]
  • Michel MF, Priem CC. Control at hospital level of infections by methicillin-resistant staphylococci in children. J Hyg (Lond) 1971 Sep;69(3):453–460. [PMC free article] [PubMed]
  • O'Toole RD, Drew WL, Dahlgren BJ, Beaty HN. An outbreak of methicillin-resistant Staphylococcus aureus infection. Observations in hospital and nursing home. JAMA. 1970 Jul 13;213(2):257–263. [PubMed]
  • PARKER MT, JEVONS MP. A SURVEY OF METHICILLIN RESISTANCE IN STAPHYLOCOCCUS AUREUS. Postgrad Med J. 1964 Dec;40:SUPPL–SUPPL:178. [PMC free article] [PubMed]
  • Peacock JE, Jr, Marsik FJ, Wenzel RP. Methicillin-resistant Staphylococcus aureus: introduction and spread within a hospital. Ann Intern Med. 1980 Oct;93(4):526–532. [PubMed]
  • Thompson RL, Cabezudo I, Wenzel RP. Epidemiology of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. Ann Intern Med. 1982 Sep;97(3):309–317. [PubMed]
  • Thornsberry C, Caruthers JQ, Baker CN. Effect of temperature on the in vitro susceptibility of Staphylococcus aureus to penicillinase-resistant penicillins. Antimicrob Agents Chemother. 1973 Sep;4(3):263–269. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...