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J Clin Microbiol. May 1984; 19(5): 645–648.
PMCID: PMC271147

Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.


Immunological tolerance is a state of unresponsiveness to foreign substances (antigens) which can develop in human and animal species as the result of continued exposure to antigens early in life. We utilized this principle for the preparation of antibodies against Clostridium botulinum type A toxin. By selective suppression of the immunological response of rabbits to unwanted antigens and subsequent immunization with a toxoid, we were able to produce a specific type A antitoxin without the need to purify the toxin. Despite cross-reactivity with C. botulinum type B, our type A antitoxin was otherwise specific since it did not react with culture filtrates of nontoxigenic variants of type B, any other C. botulinum type (C, D, E, F, and G), nor with 18 other Clostridium species, including Clostridium sporogenes. Using this antitoxin, we developed a sensitive enzyme-linked immunosorbent assay for detection of C. botulinum type A toxin.

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Selected References

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  • Boroff DA, Chu-Chen G. Radioimmunoassay for type A toxin of Clostridium botulinum. Appl Microbiol. 1973 Apr;25(4):545–549. [PMC free article] [PubMed]
  • Dezfulian M, Dowell VR., Jr Cultural and physiological characteristics and antimicrobial susceptibility of Clostridium botulinum isolates from foodborne and infant botulism cases. J Clin Microbiol. 1980 Jun;11(6):604–609. [PMC free article] [PubMed]
  • GARB S, STEIN AA, SIMS G. The production of anti-human leukemic serum in rabbits. J Immunol. 1962 Feb;88:142–152. [PubMed]
  • Hatheway CL. Laboratory procedures for cases of suspected infant botulism. Rev Infect Dis. 1979 Jul-Aug;1(4):647–651. [PubMed]
  • Horwitz MA, Hatheway CL, Dowell VR. Laboratory diagnosis of botulism complicated by pyridostigmine treatment of the patient. A method for selectively removing interfering substances from clinical specimens. Am J Clin Pathol. 1976 Oct;66(4):737–742. [PubMed]
  • Kozaki S, Dufrenne J, Hagenaars AM, Notermans S. Enzyme linked immunosorbent assay (ELISA) for detection of Clostridium botulinum type B toxin. Jpn J Med Sci Biol. 1979 Aug;32(4):199–205. [PubMed]
  • Lee WH, Riemann H. Correlation of toxic and non-toxic strains of Clostridium botulinum by DNA composition and homology. J Gen Microbiol. 1970 Jan;60(1):117–123. [PubMed]
  • Lewis GE, Jr, Kulinski SS, Reichard DW, Metzger JF. Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay. Appl Environ Microbiol. 1981 Dec;42(6):1018–1022. [PMC free article] [PubMed]
  • LOWENTHAL JP, LAMANNA C. Characterization of botulinal hemagglutination. Am J Hyg. 1953 Jan;57(1):46–59. [PubMed]
  • Notermans S, Dufrenne J, Kozaki S. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin. Appl Environ Microbiol. 1979 Jun;37(6):1173–1175. [PMC free article] [PubMed]
  • Notermans S, Dufrenne J, Kozaki S. The relation between toxicity and toxin-related-antigen contents of Clostridium botulinum types C and D cultures as determined by mouse bioassay and ELISA. Jpn J Med Sci Biol. 1982 Oct-Dec;35(5-6):203–211. [PubMed]
  • Notermans S, Dufrenne J, Schothorst M. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A. Jpn J Med Sci Biol. 1978 Feb;31(1):81–85. [PubMed]
  • Notermans S, Hagenaars AM, Kozaki S. The enzyme-linked immunosorbent assay (ELISA) for the detection and determination of Clostridium botulinum toxins A, B, and E. Methods Enzymol. 1982;84:223–238. [PubMed]
  • Sakaguchi G, Sakaguchi S, Kozaki S, Sugii S, Oishi I. Cross reaction in reversed passive hemagglutination between Clostridium botulinum type A and B toxins and its avoidance by the sue of anti-toxic component immunoglobulin isolated by affinity chromatography. Jpn J Med Sci Biol. 1974 Jun;27(3):161–172. [PubMed]
  • Sugiyama H. Clostridium botulinum neurotoxin. Microbiol Rev. 1980 Sep;44(3):419–448. [PMC free article] [PubMed]

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