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J Clin Microbiol. Nov 1991; 29(11): 2656–2660.
PMCID: PMC270399

Characterization of human papillomavirus type 66 from an invasive carcinoma of the uterine cervix.


Human papillomavirus (HPV) DNA sequences coexisting with HPV16 and HPV45 were cloned from an invasive cervical carcinoma. The cloned HPV was shown to be a novel type, named HPV66, and is related to HPV56 (an HPV detected in cervical cancer). After screening 160 anogenital biopsies, four specimens exhibited histological features of intraepithelial neoplasia and contained HPV66 sequences. Of these, three were found to be associated with another HPV type.

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Selected References

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  • Barrasso R, De Brux J, Croissant O, Orth G. High prevalence of papillomavirus-associated penile intraepithelial neoplasia in sexual partners of women with cervical intraepithelial neoplasia. N Engl J Med. 1987 Oct 8;317(15):916–923. [PubMed]
  • Beaudenon S, Kremsdorf D, Croissant O, Jablonska S, Wain-Hobson S, Orth G. A novel type of human papillomavirus associated with genital neoplasias. Nature. 1986 May 15;321(6067):246–249. [PubMed]
  • Beaudenon S, Kremsdorf D, Obalek S, Jablonska S, Pehau-Arnaudet G, Croissant O, Orth G. Plurality of genital human papillomaviruses: characterization of two new types with distinct biological properties. Virology. 1987 Dec;161(2):374–384. [PubMed]
  • Cole ST, Danos O. Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. J Mol Biol. 1987 Feb 20;193(4):599–608. [PubMed]
  • Cole ST, Streeck RE. Genome organization and nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer. J Virol. 1986 Jun;58(3):991–995. [PMC free article] [PubMed]
  • de Villiers EM. Heterogeneity of the human papillomavirus group. J Virol. 1989 Nov;63(11):4898–4903. [PMC free article] [PubMed]
  • Dürst M, Gissmann L, Ikenberg H, zur Hausen H. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812–3815. [PMC free article] [PubMed]
  • Dyson N, Howley PM, Münger K, Harlow E. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 1989 Feb 17;243(4893):934–937. [PubMed]
  • Favre M, Kremsdorf D, Jablonska S, Obalek S, Pehau-Arnaudet G, Croissant O, Orth G. Two new human papillomavirus types (HPV54 and 55) characterized from genital tumours illustrate the plurality of genital HPVs. Int J Cancer. 1990 Jan 15;45(1):40–46. [PubMed]
  • Gallahan D, Müller M, Schneider A, Delius H, Kahn T, de Villiers EM, Gissmann L. Human papillomavirus type 53. J Virol. 1989 Nov;63(11):4911–4912. [PMC free article] [PubMed]
  • Hawley-Nelson P, Vousden KH, Hubbert NL, Lowy DR, Schiller JT. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO J. 1989 Dec 1;8(12):3905–3910. [PMC free article] [PubMed]
  • Kahn T, Schwarz E, zur Hausen H. Molecular cloning and characterization of the DNA of a new human papillomavirus (HPV 30) from a laryngeal carcinoma. Int J Cancer. 1986 Jan 15;37(1):61–65. [PubMed]
  • Kremsdorf D, Jablonska S, Favre M, Orth G. Biochemical characterization of two types of human papillomaviruses associated with epidermodysplasia verruciformis. J Virol. 1982 Aug;43(2):436–447. [PMC free article] [PubMed]
  • Lörincz AT, Quinn AP, Goldsborough MD, McAllister P, Temple GF. Human papillomavirus type 56: a new virus detected in cervical cancers. J Gen Virol. 1989 Nov;70(Pt 11):3099–3104. [PubMed]
  • Lorincz AT, Temple GF, Kurman RJ, Jenson AB, Lancaster WD. Oncogenic association of specific human papillomavirus types with cervical neoplasia. J Natl Cancer Inst. 1987 Oct;79(4):671–677. [PubMed]
  • Münger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J. 1989 Dec 20;8(13):4099–4105. [PMC free article] [PubMed]
  • Naghashfar ZS, Rosenshein NB, Lorincz AT, Buscema J, Shah KV. Characterization of human papillomavirus type 45, a new type 18-related virus of the genital tract. J Gen Virol. 1987 Dec;68(Pt 12):3073–3079. [PubMed]
  • Riou G, Favre M, Jeannel D, Bourhis J, Le Doussal V, Orth G. Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA. Lancet. 1990 May 19;335(8699):1171–1174. [PubMed]
  • Schneider A, Meinhardt G, Kirchmayr R, Schneider V. Prevalence of human papillomavirus genomes in tissues from the lower genital tract as detected by molecular in situ hybridization. Int J Gynecol Pathol. 1991;10(1):1–14. [PubMed]
  • Schneider-Gädicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 1986 Sep;5(9):2285–2292. [PMC free article] [PubMed]
  • Seedorf K, Krämmer G, Dürst M, Suhai S, Röwekamp WG. Human papillomavirus type 16 DNA sequence. Virology. 1985 Aug;145(1):181–185. [PubMed]
  • Short JM, Fernandez JM, Sorge JA, Huse WD. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 1988 Aug 11;16(15):7583–7600. [PMC free article] [PubMed]
  • Smotkin D, Prokoph H, Wettstein FO. Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J Virol. 1989 Mar;63(3):1441–1447. [PMC free article] [PubMed]
  • Werness BA, Levine AJ, Howley PM. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science. 1990 Apr 6;248(4951):76–79. [PubMed]
  • Yajima H, Noda T, de Villiers EM, Yajima A, Yamamoto K, Noda K, Ito Y. Isolation of a new type of human papillomavirus (HPV52b) with a transforming activity from cervical cancer tissue. Cancer Res. 1988 Dec 15;48(24 Pt 1):7164–7172. [PubMed]
  • zur Hausen H. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Res. 1989 Sep 1;49(17):4677–4681. [PubMed]

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